期刊
NUCLEIC ACIDS RESEARCH
卷 47, 期 10, 页码 5170-5180出版社
OXFORD UNIV PRESS
DOI: 10.1093/nar/gkz225
关键词
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资金
- ANR-FIRE [ANR-17-CE12-0015]
- LabEx Saclay Plant Sciences-SPS [ANR-10-LABX-0040-SPS]
- Agence Nationale de la Recherche (ANR) [ANR-17-CE12-0015] Funding Source: Agence Nationale de la Recherche (ANR)
Homologous recombination (HR) maintains genome stability by promoting accurate DNA repair. Two recombinases, RAD51 and DMC1, are central to HR repair and form dynamic nucleoprotein filaments in vivo under tight regulation. However, the interplay between positive and negative regulators to control the dynamic assembly/disassembly of RAD51/DMC1 filaments in multicellular eukaryotes remains poorly characterized. Here, we report an antagonism between BRCA2, a well-studied positive mediator of RAD51/DMC1, and FIDGETIN-LIKE-1 (FIGL1), which we previously proposed as a negative regulator of RAD51/DMC1. Through forward genetic screen, we identified a mutation in one of the two Arabidopsis BRCA2 paralogs that suppresses the meiotic phenotypes of figl1. Consistent with the antagonistic roles of BRCA2 and FIGL1, the figl1 mutation in the brca2 background restores RAD51/DMC1 focus formation and homologous chromosome interaction at meiosis, and RAD51 focus formation in somatic cells. This study shows that BRCA2 and FIGL1 have antagonistic effects on the dynamics of RAD51/DMC1-dependent DNA transactions to promote accurate HR repair.
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