Unmodified household coffee maker assisted extraction and purification of anticancer agents from Dillenia indica fruits

Bioassay targeted, 80% aqueous ethanol crude extract of the fruits of Dillenia indica Linn, using the unmodified household coffee maker, afforded five compounds, namely betulinic acid (1), rhamnazin (2), dillenetin (3), luteolin-7-O-beta-D-glucoside (4) and hypolaetin-8-O-beta-D-glucoside (5). The crude extract, fractions and purified compounds were tested against MDA MB-231, A549 and HeLa cancer cell lines by MTT assay, using betulinic acid 1, as a positive control. Compound 3 showed the best activity against A549 (IC50 = 26.60 +/- 2.5 mu M) and HeLa cancer cell lines (IC50 =19.35 +/- 0.9 mu M), whereas compound 5 was found to show the best activity against MDA MB-231 (IC50 = 34.62 +/- 5.2 mu M) cancer cell line. These highly potent anticancer compounds obtained from the fruits of D. indica may be suitable for herbal drug development and formulations. [GRAPHICS] .

Automated summary

The bioassay of the 80% aqueous ethanol crude extract of Dillenia indica Linn fruits resulted in the identification of five compounds, with compound 3 showing the highest activity against A549 and HeLa cancer cell lines, while compound 5 exhibited the best activity against the MDA MB-231 cancer cell line. These potent anticancer compounds may be suitable for herbal drug development and formulations.