期刊
MOLECULES
卷 24, 期 8, 页码 -出版社
MDPI
DOI: 10.3390/molecules24081633
关键词
microfluidics; microfibers; cell-laden; tissue engineering
资金
- National Key Research and Development Program of China [2018YFB1105700]
- National Natural Science Foundation of China [31530024, 81672213, 31872748]
- Jiangsu Provincial Special Program of Medical Science [BL2012004]
- China Postdoctoral Science Foundation [2016M590500, 2017T100398]
- Priority Academic Program Development (PAPD) of Jiangsu Higher Education Institutions
Fibrous hydrogel scaffolds have recently attracted increasing attention for tissue engineering applications. While a number of approaches have been proposed for fabricating microfibers, it remains difficult for current methods to produce materials that meet the essential requirements of being simple, flexible and bio-friendly. It is especially challenging to prepare cell-laden microfibers which have different structures to meet the needs of various applications using a simple device. In this study, we developed a facile two-flow microfluidic system, through which cell-laden hydrogel microfibers with various structures could be easily prepared in one step. Aiming to meet different tissue engineering needs, several types of microfibers with different structures, including single-layer, double-layer and hollow microfibers, have been prepared using an alginate-methacrylated gelatin composite hydrogel by merely changing the inner and outer fluids. Cell-laden single-layer microfibers were obtained by subsequently seeding mouse embryonic osteoblast precursor cells (MC3T3-E1) cells on the surface of the as-prepared microfibers. Cell-laden double-layer and hollow microfibers were prepared by directly encapsulating MC3T3-E1 cells or human umbilical vein endothelial cells (HUVECs) in the cores of microfibers upon their fabrication. Prominent proliferation of cells happened in all cell-laden single-layer, double-layer and hollow microfibers, implying potential applications for them in tissue engineering.
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