Article
Microbiology
Forrest K. Jones, Taufiqur R. Bhuiyan, Rachel E. Muise, Ashraful Khan, Damien M. Slater, Kian Robert Hutt Vater, Fahima Chowdhury, Meagan Kelly, Peng Xu, Pavol Kovac, Rajib Biswas, Mohammad Kamruzzaman, Edward T. Ryan, Stephen B. Calderwood, Regina C. LaRocque, Justin Lessler, Richelle C. Charles, Daniel T. Leung, Firdausi Qadri, Jason B. Harris, Andrew S. Azman
Summary: Cross-sectional serologic profiles measured with a high-throughput multiplex bead assay can accurately identify individuals infected with pandemic Vibrio cholerae O1, allowing for estimates of seroincidence. This scalable cholera serosurveillance tool provides a new avenue for understanding the epidemiology of cholera, identifying priority areas for prevention/control efforts, and tracking progress in the fight against this ancient disease.
Article
Microbiology
Daisy R. Roy, Troy J. Kemp, Katarzyna Haynesworth, Sarah A. Loftus, Ligia A. Pinto
Summary: The SARS-CoV-2 pandemic has led to the development and validation of multiple serology tests with varying performance. We have developed and validated a Luminex bead-based multiplex serology assay that can simultaneously detect antibodies against multiple SARS-CoV-2 variants and other coronaviruses, with high sensitivity and specificity. This serology assay is important for assessing SARS-CoV-2 infection and vaccine immune responses.
MICROBIOLOGY SPECTRUM
(2023)
Article
Immunology
Vishal Rathod, Laxmikant Kadam, Manish Gautam, Prabhu Dasu Gumma, Kevin Marke, Cathy Asokanathan, Alex Douglas-Bardsley, Laura Hassell, Sachin Bhandare, Sumit Gupta, Sameer Parekh, Pramod Pujari, Harish Rao, Hitt Sharma, Umesh Shaligram, Sunil Gairola
Summary: This study developed and validated a multiplex immunoassay (MIA) for the simultaneous estimation of IgG antibodies against pertussis toxin, filamentous hemagglutinin, pertactin, diphtheria toxoid, and tetanus toxoid. The MIA was calibrated using WHO reference standards and complied with US FDA, EMA, and ICH M10 guidelines. It showed good agreement with commercial ELISA assays and a positive correlation with toxin neutralization assays for pertussis and diphtheria.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Libo Zhang, Hai Yu, Yuanyuan Bai, Bijoyananda Mishra, Xiaoxiao Yang, Jing Wang, Evan B. Yu, Riyao Li, Xi Chen
Summary: Carbohydrate-protein conjugates have diverse applications, from vaccines to high-throughput assays. A process combining chemoenzymatic synthesis, squarate linker conjugation, and neoglycoprotein immobilization on fluorescent magnetic beads was developed for a suspension multiplex array platform. This platform allowed for analysis of glycan and GBP interactions, providing useful insights into human galectins' multivalent glycan-binding properties.
Article
Biochemical Research Methods
Evgeniia N. Bykonia, Denis A. Kleymenov, Elena P. Mazunina, Liubov I. Popova, Victor A. Manuylov, Vladimir A. Gushchin, Artem P. Tkachuk, Alexander L. Gintsburg
Summary: Serosurveillance and seroprevalence studies are important for monitoring vaccine-preventable diseases, and multiplex immunoassay systems offer advantages over conventional methods. A developed multiplex immunoassay for seven vaccine-preventable infections showed good correlation with ELISAs and high sensitivity and specificity for determining serostatus. This in-house MIA is a feasible alternative to ELISAs and suitable for large-scale serosurveillance/seroprevalence studies.
JOURNAL OF IMMUNOLOGICAL METHODS
(2023)
Article
Microbiology
Yong Wang, TaLesa Aderohunmu, Henry Bishop, Isabel McAuliffe, Hilda N. Rivera, Darlyne Smith, Patricia P. Wilkins, Katherine E. Bowden, Matthew S. Reed, Pavel Svoboda, Olga Stuchlik, Jan Pohl, Ryan E. Wiegand, Sukwan Handali
Summary: Babesia duncani is the causative agent of babesiosis in the western United States. The diagnostic test of choice for detection of B. duncani-specific antibodies is the indirect fluorescent antibody (IFA) assay, which has limitations in interpretation. A proteomics approach was employed to identify candidate immunodiagnostic antigens, resulting in two antigens with high sensitivities and specificities for detecting B. duncani-specific antibodies.
JOURNAL OF CLINICAL MICROBIOLOGY
(2021)
Article
Veterinary Sciences
Sanne Schou Berger, Ulla Riber, Vibeke Frokjaer Jensen, Klara Tolboll Lauritsen, Lars Ole Andresen
Summary: A bead-based MFIA was developed for the detection of Salmonella antibodies in pig serum, which has important implications for the Danish Salmonella surveillance program.
BMC VETERINARY RESEARCH
(2022)
Article
Veterinary Sciences
Fimme J. van der Wal, Rene P. Achterberg, Jeanet A. van der Goot, Annemieke Dinkla, Ruth Bossers-de Vries, Conny van Solt-Smits, Alex Bossers, Marloes Heijne
Summary: The study focused on developing a differentiating serological test for antibodies against Chlamydia species in chicken sera. A bead-based Luminex suspension array was constructed using peptides as antigens, which correctly identified antibodies and differentiated between different Chlamydia species in experimentally infected mice and chickens. Although interpretation of signals in field sera was difficult due to limited samples, results were supported by published data.
VETERINARY RESEARCH
(2023)
Article
Infectious Diseases
Heather M. Scobie, Nino Khetsuriani, Androulla Efstratiou, Jeffrey W. Priest
Summary: The study validated a multiplex bead assay for diphtheria toxoid IgG antibodies against the Vero cell toxin neutralization test, showing a high correlation. Sensitivity was 95% and specificity was 83% at a cutoff of >0.01 IU/mL for minimal seroprotection, with a high agreement across three categories of IU/mL values.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2021)
Article
Immunology
Garima Shrinet, Rajesh Chhabra, Archana Sharma, Kanisht Batra, Saurabh Jyoti Talukdar, Sushila Maan
Summary: In this study, a Luminex beads-based multiplex assay was developed for the precise identification of the six major bacterial pathogens causing bovine mastitis. The assay demonstrated 100% analytical specificity for simultaneous detection of these bacteria, and the sensitivity was comparable to other real-time PCR multiplex assays. This assay provides a concept proof for a rapid, cost-effective, and high-throughput diagnostic tool for identifying major bacteria causing mastitis.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2023)
Article
Public, Environmental & Occupational Health
Jeffrey W. Priest, Sukwan Handali
Summary: Recent large-scale serologic surveys in countries where human African trypanosomiasis (HAT) remains endemic could help better map disease foci and identify asymptomatic individuals infected with the chronic form of Trypanosoma brucei gambiense (gHAT). Incorporating two antigens into a multiplex bead assay has shown high sensitivity and specificity for detecting gHAT infection, with potential for improving sensitivity for the acute form caused by T.b. rhodesiense (rHAT) by adding more antigens in the future.
AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE
(2021)
Article
Microbiology
Antonia Touloudi, George Valiakos, Shaun Cawthraw, Polychronis Kostoulas, Christian Gortazar, Mariana Boadella, Alexios Giannakopoulos, Periklis Birtsas, Marina Sofia, Labrini V. Athanasiou, Maria Satra, Zoi Athanasakopoulou, Maria Kantere, Vassiliki Spyrou, Liljana Petrovska, Charalambos Billinis
Summary: The multiplex bead assay showed high sensitivity and specificity for the detection of antibodies against Mycobacterium bovis, Brucella suis, and Trichinella spiralis in Eurasian wild boar. The assay has great diagnostic potential for simultaneous detection of these antibodies.
Article
Cell Biology
Gonca E. Karahan, Geert W. Haasnoot, Kim Voogt-Bakker, Frans H. J. Claas, Dave Roelen, Sebastiaan Heidt
Summary: This study proposes a non-linear modeling approach to accurately convert MFI values between two vendors and establish user-independent MFI cutoffs. The method was validated using 47 EDTA-treated serum samples, showing a 94% accuracy in MFI values between the two vendors.
Article
Infectious Diseases
Kimberly R. R. Celona, Austin B. B. Shannon, Derek Sonderegger, Jinhee Yi, Fernando P. P. Monroy, Christopher Allender, Heidie Hornstra, Mary B. B. Barnes, Elizabeth S. S. Didier, Rudolf P. P. Bohm, Kathrine Phillippi-Falkenstein, Daniel Sanford, Paul Keim, Erik W. W. Settles
Summary: This study evaluated the diagnostic potential of the BurkPx assay, a multiple antigen serological test, for detecting Burkholderia pseudomallei exposure in non-human primates (NHP). The results showed early IgM antibody response to carbohydrate antigens followed by IgG antibody recognition to multiple B. pseudomallei protein antigens during the second week of infection. The use of a multiple antigen model resulted in 100% specificity and 63.48% sensitivity, which increased to 95% sensitivity after excluding the week 1 serum samples challenged with B. pseudomallei.
PLOS NEGLECTED TROPICAL DISEASES
(2023)
Article
Microbiology
Kaitlin F. Mitchell, Christina M. Carlson, Douglas Nace, Brian S. Wakeman, Jan Drobeniuc, Glenn P. Niemeyer, Bonnie Werner, Alex R. Hoffmaster, Panayampalli S. Satheshkumar, Amy J. Schuh, Venkatachalam Udhayakumar, Eric Rogier
Summary: Reporting of SARS-CoV-2 infections through nucleic acid or antigen based diagnostic tests severely underestimates the true burden of exposure in a population. Serological data assaying for antibodies against SARS-CoV-2 antigens offers an alternative source of data to estimate population exposure. This study evaluated a multiplex assay that detects antibodies to three SARS-CoV-2 targets and found that it had high diagnostic sensitivity and specificity.
MICROBIOLOGY SPECTRUM
(2022)
Meeting Abstract
Biophysics
Meagan C. Small, Deborah A. Sarkes, Hong Dong, Dimitra N. Stratis-Cullum, Margaret M. Hurley
BIOPHYSICAL JOURNAL
(2019)
Article
Engineering, Biomedical
Jose A. Wippold, Can Huang, Dimitra Stratis-Cullum, Arum Han
BIOMEDICAL MICRODEVICES
(2020)
Article
Chemistry, Multidisciplinary
Maiko Furubayashi, Andrea K. Wallace, Lina M. Gonzalez, Justin P. Jahnke, Brendan M. Hanrahan, Alexis L. Payne, Dimitra N. Stratis-Cullum, Matthew T. Gray, Han Liu, Melissa K. Rhoads, Christopher A. Voigt
Summary: This study explores the genetic control of iron oxide nanoparticles (IONPs) properties, such as size, morphology, chain length, and surface coating, through genetic engineering, introducing designed control systems and inducible pathways.
ADVANCED FUNCTIONAL MATERIALS
(2021)
Article
Engineering, Biomedical
Can Huang, Jose A. Wippold, Dimitra Stratis-Cullum, Arum Han
BIOMEDICAL MICRODEVICES
(2020)
Article
Nanoscience & Nanotechnology
Jessica L. Terrell, Tanya Tschirhart, Justin P. Jahnke, Kristina Stephens, Yi Liu, Hong Dong, Margaret M. Hurley, Maria Pozo, Ryan McKay, Chen Yu Tsao, Hsuan-Chen Wu, Gary Vora, Gregory F. Payne, Dimitra N. Stratis-Cullum, William E. Bentley
Summary: Living electrodes facilitate signaling within microbial communities, coordinating behavior. This study developed a bioelectronic communication system that allows exchange of information between living cells and an engineered microbial network, enabling real-time control and feedback. The system demonstrates how molecular communication can be propagated to drive biological signal propagation and programmed tasks concurrently.
NATURE NANOTECHNOLOGY
(2021)
Article
Biophysics
Hong Dong, Deborah A. Sarkes, Dimitra N. Stratis-Cullum, Margaret M. Hurley
Summary: In this study, a method for direct conjugation of carboxyl quantum dots with E. coli outer membrane using binding peptides was reported. This method allows for high loading densities of QDs on the cell surface, enabling the cells to fluoresce with different colors. The approach is simple, direct, and efficient, while maintaining the viability of the bacteria.
COLLOIDS AND SURFACES B-BIOINTERFACES
(2021)
Article
Energy & Fuels
Justin P. Jahnke, Deborah A. Sarkes, Jessica L. Liba, James J. Sumner, Dimitra N. Stratis-Cullum
Summary: Microorganism surface affinity can be controlled by introducing material binding motifs into proteins, enhancing electrical power production in a microbial fuel cell. Strains with higher affinity for gold surfaces generate higher peak voltages and power densities in MFCs, showing a clear correlation between surface affinity and power generation.
Article
Materials Science, Multidisciplinary
Song- Han, Deborah A. Sarkes, Justin P. Jahnke, Margaret M. Hurley, Victor M. Ugaz, Rebecca L. Renberg, James J. Sumner, Dimitra N. Stratis-Cullum, Arum Han
Summary: This study presents a centrifugal force-based microfluidic biopanning platform for screening peptides with specific affinity towards target materials, allowing for precise control of wash steps by adjusting rotation speed. Through iterative sorting, strong affinity isolates towards gold, ITO or both are successfully obtained. Amino acid analysis provides insights into the distinctive attributes of these peptides and their material-binding capabilities. The platform is user-friendly and versatile, enabling rapid discovery of peptide-driven biomaterials and hybrid organic-inorganic materials.
ADVANCED MATERIALS TECHNOLOGIES
(2021)
Article
Nanoscience & Nanotechnology
Song- Han, Deborah A. Sarkes, Margaret M. Hurley, Rebecca Renberg, Can Huang, Yuwen Li, Justin P. Jahnke, James J. Sumner, Dimitra N. Stratis-Cullum, Arum Han
Summary: Discovery of microorganisms and their relevant surface peptides that specifically bind to target materials can be achieved through iterative biopanning-based screening. Recently, microfluidics-based biopanning methods have been developed to overcome the limitations of conventional methods. In this work, a magnetophoretic microfluidic biopanning platform was developed to isolate microorganisms that bind to gold, and successful screening of bacterial peptide display library and soil microbes was demonstrated.
ACS APPLIED MATERIALS & INTERFACES
(2023)
Article
Chemistry, Analytical
Rabeb Layouni, Tengfei Cao, Matthew B. Coppock, Paul E. Laibinis, Sharon M. Weiss
Summary: Research shows that peptides as bioreceptors offer higher stability and reliability in harsh environments compared to monoclonal antibodies. The study demonstrates the functionalization of peptides by capturing the Chikungunya virus E2 protein on a biosensor even under high temperature conditions.
Meeting Abstract
Biophysics
Meagan C. Small, Deborah A. Sarkes, Hong Dong, Jessica L. Terrell, Justin P. Jahnke, Theresah Zu, Dimitra N. Stratis-Cullum, Margaret M. Hurley
BIOPHYSICAL JOURNAL
(2021)
Proceedings Paper
Engineering, Biomedical
Tengfei Cao, Rabeb Layouni, Matthew B. Coppock, Paul E. Laibinis, Sharon M. Weiss
FRONTIERS IN BIOLOGICAL DETECTION: FROM NANOSENSORS TO SYSTEMS XII
(2020)
Meeting Abstract
Chemistry, Multidisciplinary
Hong Dong, Jessica Terrell, Justin Jahnke, Margaret Hurley, Dimitra Stratis-Cullum, Theresah Zu
ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY
(2019)
Proceedings Paper
Chemistry, Applied
Matthew B. Coppock, Dimitra N. Stratis-Cullum
SMART BIOMEDICAL AND PHYSIOLOGICAL SENSOR TECHNOLOGY XV
(2019)
Article
Biochemical Research Methods
K. Ramki, G. Thiruppathi, Selva Kumar Ramasamy, P. Sundararaj, P. Sakthivel
Summary: A chromone-based ratiometric fluorescent probe L2 was developed for the selective detection of Hg(II) in a semiaqueous solution. The probe exhibited enhanced fluorescence in its aggregated state and even higher fluorescence when chelated with Hg(II). The probe demonstrated high sensitivity and specificity for Hg(II) detection and was successfully applied for imaging Hg(II) in a living model.
Article
Biochemical Research Methods
Qun Zhang, Rui Yang, Gang Liu, Shiyan Jiang, Jiarui Wang, Juqiang Lin, Tingyin Wang, Jing Wang, Zufang Huang
Summary: This research aims to develop a cost-effective and portable method for measuring creatinine levels using the enhanced Tyndall effect phenomenon. The method offers a promising solution for monitoring renal healthcare in resource-limited settings.
