期刊
MARINE POLLUTION BULLETIN
卷 142, 期 -, 页码 384-393出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.marpolbul.2019.03.003
关键词
Microplastics; Digestion; Improved filterability; Raman spectroscopy; Small particles; Bivalves
资金
- EPSRC
- Leverhulme Trust
- Blue Marine Foundation, London, UK
- Engineering and Physical Sciences Research Council [1790027] Funding Source: researchfish
Methods standardisation in microplastics research is needed. Apart from reagent-dependent effects on micro plastics, varying target particle sizes can hinder result comparison between studies. Human health concerns warrant recovery of small microplastics. We compared existing techniques using hydrogen peroxide, Proteinase-K, Trypsin and potassium hydroxide to digest bivalve tissue. Filterability, digestion efficacy, recoverability of microplastics and subsequent polymer identification using Raman spectroscopy and a matching software were assessed. Only KOH allowed filtration at <= 25 mu m. When adding a neutralisation step prior to filtration, KOH digestates were filterable using 1.2-pm filters. Digestion efficacies were >95.0% for oysters, but lower for clams. KOH destroyed rayon at 60 degrees C but not at 40 degrees C. Acrylic fibre identification was affected due to changes in Raman spectra peaks. Despite those effects, we recommend KOH as the most viable extraction method for exposure risk studies, due to microplastics recovery from bivalve tissues of single-digit micrometre size.
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