Article
Biotechnology & Applied Microbiology
Sabrina Amalfi, Maria del Pilar Plastine, Maria Gabriela Lopez, Maria Jose Gravisaco, Oscar Taboga, Victoria Alfonso
Summary: In this study, researchers demonstrated that the poxin homologous P26 from AcMNPV can degrade 2'3'-cGAMP, thus inhibiting the antiviral response and improving gene delivery efficiency. Incorporating P26 into baculoviral vectors significantly increased the efficiency of transgene expression.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2023)
Editorial Material
Microbiology
Tom Gallagher
Summary: A recent study has identified protein complexes, including VPS29, that play a crucial role in creating favorable environments for virus entry into endocytic vesicles. Without VPS29, endosomes lack the necessary protease activities to support the entry of certain viruses. These findings provide valuable insights into the virus-host interactions and may have implications for developing targeted therapies against coronaviruses.
Article
Biochemistry & Molecular Biology
Sajedah M. Hindi, Michael J. Petrany, Elena Greenfeld, Leah C. Focke, Alyssa A. W. Cramer, Michael A. Whitt, Ramzi J. Khairallah, Christopher W. Ward, Jeffrey S. Chamberlain, Benjamin Podbilewicz, Vikram Prasad, Douglas P. Millay
Summary: The muscle-specific fusogens Myomaker and Myo-merger can replace viral fusogens to mediate membrane fusion and deliver therapeutic genes to skeletal muscle. By engineering these muscle fusogens onto enveloped viruses, targeted transduction of skeletal muscle is achieved, providing a potential treatment for Duchenne muscular dystrophy.
Review
Virology
Stefan Windhaber, Qilin Xin, Pierre-Yves Lozach
Summary: Orthobunyavirus, the largest genus in the Peribunyaviridae family with over 80 members worldwide, is a structurally simple arthropod-borne virus with a trisegmented, negative-sense RNA genome and only four structural proteins. As potential agents of emerging and re-emerging diseases, OBVs pose a global threat to both public and veterinary health. This review focuses on the initial stages of OBV infection in mammalian hosts, covering virus binding, penetration, and release of the viral genome into the cytosol, and discusses the current understanding of OBV receptors, endocytosis, and fusion.
Article
Cell Biology
Sonja Fernbach, Eva E. Spieler, Idoia Busnadiego, Umut Karakus, Anouk Lkharrazi, Silke Stertz, Benjamin G. Hale
Summary: Using RNAi screening, researchers identified RABGAP1L as a key factor in IFN-mediated immune defense, which restricts viral infection by disrupting the cell entry process.
Review
Virology
Jana Koch, Qilin Xin, Nicole D. Tischler, Pierre-Yves Lozach
Summary: Phenuiviridae is a large family of arthropod-borne viruses, some of which may become potential factors for emerging diseases. This family of viruses early infection in mammalian host cells includes the process from virus binding to penetration into the cytosol.
Article
Multidisciplinary Sciences
Jason Allen, Jean-Philippe Pellois
Summary: To deliver useful biological payloads into cells, cell-penetrating peptides (CPPs) need to cross biological membranes. This study found that incorporating groups with higher hydrophobicity can enhance the delivery activity of CPP conjugates, leading to intracellular delivery. The chemical structures of the hydrophobic groups do not affect cell penetration activity, which is consistent with endosomal escape and membrane permeation.
SCIENTIFIC REPORTS
(2022)
Article
Biochemistry & Molecular Biology
Wenjun Guo, Mengmeng Wang, Lei Chen
Summary: The BacMam system, utilizing baculovirus as a delivery tool, is widely used for recombinant production of eukaryotic proteins in mammalian cells. In this study, a BacMam vector (pBMCL1) was developed, which allows easy tracking of virus production, enhances infection efficiency in mammalian cells, reduces unwanted transcription of toxic genes in insect cells, and enables the co-expression of multiple proteins using a single virus. The successful application of the pBMCL1 vector in expressing homo-tetrameric human TRPC3 channel and hetero-octameric KATP channel is demonstrated.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2022)
Review
Pharmacology & Pharmacy
Hanan Bloomer, Jennifer Khirallah, Yamin Li, Qiaobing Xu
Summary: The CRISPR/Cas system has revolutionized the ability to edit the mammalian genome, providing a platform for correcting pathogenic mutations and studying gene function. Cas9 RNPs, as a delivery method, offer advantages in editing efficiency, off-target editing activity, and cellular toxicity reduction.
ADVANCED DRUG DELIVERY REVIEWS
(2022)
Article
Multidisciplinary Sciences
Ross D. Jones, Yili Qian, Katherine Ilia, Benjamin Wang, Michael T. Laub, Domitilla Del Vecchio, Ron Weiss
Summary: Phosphorylation networks play a crucial role in complex cellular decision making. This study engineers synthetic phosphorylation devices with feedback regulation in mammalian cells and demonstrates their tunable and robust control over cell behaviors. By using bacterial two-component signaling proteins, the authors develop synthetic phosphoregulation devices that can precisely regulate target gene expression and withstand perturbations in mammalian cells. The work lays the foundation for establishing tunable, precise, and robust control over cell behavior with synthetic signaling networks.
NATURE COMMUNICATIONS
(2022)
Article
Multidisciplinary Sciences
Hui-Chung Lin, Der-Jiang Chiao, Chang-Chi Lin, Szu-Cheng Kuo
Summary: The study demonstrates efficient delivery of CHIKV replicons into cells using a baculovirus, verifying its efficacy through experiments, offering insights into virus replication mechanisms and potential for antiviral development.
SCIENTIFIC REPORTS
(2021)
Article
Virology
Bifang Hao, Chenya Li, Charles Amanze, Jinshan Huang
Summary: This study suggests that ERAPs may play an important role in the membrane fusion process during Bombyx mori nucleopolyhedrovirus (BmNPV) infection.
ARCHIVES OF VIROLOGY
(2022)
Article
Multidisciplinary Sciences
M. Maschietto, M. Dal Maschio, S. Girardi, S. Vassanelli
Summary: Electroporation is a widely utilized technique for non-viral delivery of molecules into cells, and recent advancements have miniaturized the machinery for in-situ genetic manipulation with high spatial selectivity and cell viability, even with differentiated neurons. SiO2 thin film-insulated microelectrodes support reliable and spatially selective electroporation and enable double and serial transfection of targeted cells.
SCIENTIFIC REPORTS
(2021)
Article
Multidisciplinary Sciences
Ronghui Zhu, Jesus M. del Rio-Salgado, Jordi Garcia-Ojalvo, Michael B. Elowitz
Summary: This study explores synthetic circuits that generate multiple stable states, providing insights into natural cell fate control circuit structures and enabling engineering of multicellular programs requiring interactions among distinct cell types.
Article
Virology
Sandra R. Abbo, Wilson Nguyen, Marleen H. C. Abma-Henkens, Denise van de Kamer, Niek H. A. Savelkoul, Corinne Geertsema, Thuy T. T. Le, Bing Tang, Kexin Yan, Troy Dumenil, Monique M. van Oers, Andreas Suhrbier, Gorben P. Pijlman
Summary: Mayaro virus is associated with acute rheumatic disease and has the potential to emerge as a tropical public health threat. Researchers developed a virus-like particle vaccine against Mayaro virus, which induced neutralizing antibodies and protected mice against infection and disease.
JOURNAL OF VIROLOGY
(2023)