期刊
JOURNAL OF NEUROSCIENCE
卷 39, 期 29, 页码 5794-5815出版社
SOC NEUROSCIENCE
DOI: 10.1523/JNEUROSCI.2724-18.2019
关键词
dentate granule cells (DGCs); DREADD; environmental enrichment; frontotemporal dementia (FTD); rabies virus; RGB retrovirus
资金
- Spanish Ministry of Economy and Competitiveness [SAF-2017-82185-R, RYC-2015-171899, SAF-2014-53040-P]
- Alzheimer's Association [2015-NIRG-340709, AARG-17-528125]
- Association for Frontotemporal Degeneration (2016 Basic Science Pilot Grant Award)
- Comunidad de Madrid [PEJD-2017-PRE/BMD-3439]
- Center for Networked Biomedical Research on Neurodegenerative Diseases (CIBERNED)
- Argentine Agency for the Promotion of Science and Technology [PICT2015-3814, PICT2013-1685]
- Howard Hughes Medical Institute (SIRS) [55007652]
- National Institute on Aging (Intramural Research Program)
- Fundacion Ramon Areces
- Banco de Santander
- Universidad Autonoma de Madrid [FPI-UAM-2017]
- [PRE2018-085233]
Frontotemporal dementia (FTD) is characterized by neuronal loss in the frontal and temporal lobes of the brain. Here, we provide the first evidence of striking morphological alterations in dentate granule cells (DGCs) of FTD patients and in a mouse model of the disease, Tau(VLW) mice. Taking advantage of the fact that the hippocampal dentate gyrus (DG) gives rise to newborn DGCs throughout the lifetime in rodents, we used RGB retroviruses to study the temporary course of these alterations in newborn DGCs of female Tau(VLW) mice. In addition, retroviruses that encode either PSD95:GFP or Syn:GFP revealed striking alterations in the afferent and efferent connectivity of newborn Tau(VLW) DGCs, and monosynaptic retrograde rabies virus tracing showed that these cells are disconnected from distal brain regions and local sources of excitatory innervation. However, the same cells exhibited a predominance of local inhibitory innervation. Accordingly, the expression of presynaptic and postsynaptic markers of inhibitory synapses was markedly increased in the DG of Tau(VLW) mice and FTD patients. Moreover, an increased number of neuropeptide Y-positive interneurons in the DG correlated with a reduced number of activated egr-1(+) DGCs in Tau(VLW) mice. Finally, we tested the therapeutic potential of environmental enrichment and chemoactivation to reverse these alterations in mice. Both strategies reversed the morphological alterations of newborn DGCs and partially restored their connectivity in a mouse model of the disease. Moreover, our data point to remarkable morphological similarities between the DGCs of Tau(VLW) mice and FTD patients.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据