Article
Optics
N. R. Subedi, S. Yaraghi, P. S. Jung, G. Kukal, A. G. McDonald, D. N. Christodoulides, A. E. Vasdekis
Summary: Research shows that digitally scanned Airy beams can improve the throughput rates in Raman imaging by an order of magnitude compared to traditional diffraction-limited beams, achieving high contrast and 1 micron axial resolution for three-dimensional imaging of microparticles. This method also achieves orders of magnitude lower irradiation density than coherent Raman imaging schemes, with faster speed and lower phototoxicity.
Article
Biochemical Research Methods
Petra Paie, Gianmaria Calisesi, Alessia Candeo, Andrea Comi, Federico Sala, Francesco Ceccarelli, Ada De Luigi, Pietro Veglianese, Korbinian Muhlberger, Michael Fokine, Gianluca Valentini, Roberto Osellame, Mark Neil, Andrea Bassi, Francesca Bragheri
Summary: Heterogeneity investigation at the single-cell level is crucial in clinical research. The combination of light sheet fluorescence microscopy and structured illumination microscopy in an optofluidic platform enables high throughput super-resolution imaging, allowing comprehensive evaluation of cellular heterogeneity at high resolution.
Article
Biochemical Research Methods
Kevin M. Dean, Tonmoy Chakraborty, Stephan Daetwyler, Jinlong Lin, Gerard Garrelts, Ons M'Saad, Hannahmariam T. Mekbib, Fabian F. Voigt, Martina Schaettin, Esther T. Stoeckli, Fritjof Helmchen, Joerg Bewersdorf, Reto Fiolka
Summary: The protocol provides detailed instructions for assembling and operating a versatile variant of light-sheet fluorescence microscopy called axially swept light-sheet microscopy (ASLM), which offers an unparalleled combination of field of view, optical resolution, and optical sectioning. It includes information on the working principle, applications, practical tips, part lists, schematics, and software for instrument control of ASLM.
Review
Optics
Xi Chen, Mikhail E. Kandel, Gabriel Popescu
Summary: This paper introduces the principles, imaging mechanisms, operational requirements, and applications of Spatial Light Interference Microscopy (SLIM) in basic science and clinical research. SLIM is a highly sensitive quantitative phase imaging method that can assist in studying cell dynamics, cancer research, and more.
ADVANCES IN OPTICS AND PHOTONICS
(2021)
Article
Optics
S. Crombez, P. Leclerc, C. Ray, N. Ducros
Summary: We present a computational light-sheet microscope that enables hyperspectral acquisition at high spectral resolution. By focusing the emitted fluorescence light onto the entrance slit of an imaging spectrometer using a cylindrical lens, and illuminating the specimen with a sequence of structured light patterns to capture the spatial dimension orthogonal to the slit, we demonstrate the feasibility of this method and report the initial results in vivo using hydra specimens labeled with two fluorophores.
Article
Nanoscience & Nanotechnology
Yuan Luo, Ming Lun Tseng, Sunil Vyas, Ting-Yu Hsieh, Jui-Ching Wu, Shang-Yang Chen, Hsiao-Fang Peng, Vin-Cent Su, Tzu-Ting Huang, Hsin Yu Kuo, Cheng Hung Chu, Mu Ku Chen, Jia-Wern Chen, Yu-Chun Chen, Kuang-Yuh Huang, Chieh-Hsiung Kuan, Xu Shi, Hiroaki Misawa, Din Ping Tsai
Summary: Light-sheet fluorescent microscopy (LSFM) is the leading technique for in vivo imaging in the fields of disease, medicine, and cell biology research. This study demonstrates the successful integration of a nanophotonic meta-lens as the illumination component for LSFM, addressing the challenges of high image resolution and optical sectioning. With the meta-lens, the LSFM system complexity is significantly reduced, enabling multicolor fluorescent imaging of live biological specimens with cellular resolution.
Article
Biology
Max Hahn, Christoffer Nord, Maria Eriksson, Federico Morini, Tomas Alanentalo, Olle Korsgren, Ulf Ahlgren
Summary: This study presents a simple approach to reconstruct antibody labeled cells within entire human organs with preserved organ context, demonstrating previously unrecognized features of human islet mass distribution and pathology in the pancreas. The method may be translated to essentially any antibody marker or organ system, providing valuable insights into pancreatic anatomy/pathophysiology and potentially revealing new information on diseases like type 2 diabetes.
COMMUNICATIONS BIOLOGY
(2021)
Article
Plant Sciences
Helena Vernet, Aida Magdalena Fullana, Francisco Javier Sorribas, Emilio J. Gualda
Summary: Plant-parasitic nematodes are a significant threat to crop yield and food security. Imaging techniques that visualize the galls induced by nematodes can provide valuable insights into their control. This study aims to identify the most appropriate clearing protocol and microscopy system for obtaining 3D images of tomato and eggplant samples infected with Meloidogyne incognita, and also compares alternative imaging techniques with confocal microscopy.
Article
Biochemical Research Methods
Biagio Mandracchia, Jeonghwan Son, Shu Jia
Summary: The study introduced an optofluidic scanning microscopy (OSM) system for super-resolution, live-cell imaging, utilizing multi-focal excitation through the innate fluidic motion of specimens to achieve effective resolution doubling, optical sectioning, and contrast enhancement. The system features minimal instrumental complexity and full compatibility with various microfluidic configurations, offering a promising super-resolution optofluidic paradigm for miniaturization and different levels of integration at the chip scale.
Article
Optics
Reddikumar Maddipatla, Patrice Tankam
Summary: Combining optical coherence microscopy with fluorescence microscopy allows for the alignment of structural and functional information, enabling new investigations into biological processes in animal models.
OPTICS AND LASERS IN ENGINEERING
(2022)
Article
Optics
Anne Stockhausen, Juan Eduardo Rodriguez-Gatica, Jens Schweihoff, Martin Karl Schwarz, Ulrich Kubitscheck
Summary: Common light sheet microscopy has limitations in balancing light sheet width and usable field of view, which can be overcome by using low-diverging Airy beams. However, Airy beams have side lobes that degrade image contrast. In this study, we constructed an Airy beam light sheet microscope and developed a deep learning image deconvolution method to remove side lobe effects. The combination of Airy beam light sheet microscopy and deep learning deconvolution allows for rapid and high-quality imaging of large volumes.
Article
Developmental Biology
Mostafa Aakhte, Hans-Arno J. Mueller
Summary: Light-sheet or selective plane illumination microscopy (SPIM) is suitable for in toto imaging of living specimens at high temporal-spatial resolution, but faces limitations due to light scattering in opaque specimens. The Multiview tiling SPIM (MT-SPIM) combines the strengths of Multi-view SPIM (M-SPIM) and a confined, multi-tiled light sheet, providing high-resolution, robust, and rotation-free imaging of living specimens. It improves axial resolution by a factor of two, enhancing automated segmentation of cellular structures.
Article
Multidisciplinary Sciences
Marco Leonetti, Lorenzo Pattelli, Simone De Panfilis, Diederik S. Wiersma, Giancarlo Ruocco
Summary: Through directly measuring spatially resolved intensity correlations of light inside a disordered medium using DNA strings decorated with emitters, researchers uncovered deviations in the size and polarization degrees of freedom of bulk speckles from theoretical predictions. These deviations are explained by correlations among polarization components and non-universal near-field contributions at the nanoscale.
NATURE COMMUNICATIONS
(2021)
Article
Biochemical Research Methods
Nava R. R. Subedi, Sergey Stolyar, Sabrina J. J. Tuson, Christopher J. J. Marx, Andreas E. E. Vasdekis
Summary: Scattering-based light-sheet microscopy has become an important technique in label-free tissue imaging and cellular morphometry, but achieving subcellular resolution remains a challenge. In this study, we addressed this by using time-averaged pseudo-thermalized light-sheet illumination, which allowed us to achieve subcellular resolving power after image deconvolution. We successfully validated this approach by imaging cytosolic carbon depots in yeast and bacteria with increased specificity, no staining, and ultralow irradiance levels. This scattering-based light-sheet microscopy approach has the potential to advance single, live cell imaging by reducing phototoxicity.
JOURNAL OF BIOPHOTONICS
(2023)
Review
Endocrinology & Metabolism
Tomas Alanentalo, Max Hahn, Stefanie M. A. Willekens, Ulf Ahlgren
Summary: The exocrine-endocrine multipart organization of the pancreas presents challenges in quantitative and spatial analysis. High-resolution optical imaging techniques show potential in providing detailed 3D spatial, quantitative, and molecular information for better understanding the pancreatic anatomy.
FRONTIERS IN ENDOCRINOLOGY
(2021)