Review
Plant Sciences
Anqi Zhou, Kang Zhou, Yanran Li
Summary: This review highlights the potential of microbial bioproduction as an alternative sourcing to plant natural products, focusing on strategies to enhance the activity of plant CYPs in Escherichia coli and Saccharomyces cerevisiae. In-depth investigations on the native microenvironment of plant CYPs are essential for further improving the efficiency of functional reconstitution of plant CYPs in microbial systems.
CURRENT OPINION IN PLANT BIOLOGY
(2021)
Review
Plant Sciences
Xu Lu, Lijin Huang, Henrik Scheller, Jay D. Keasling
Summary: Terpenoid glycosides derived from medicinal plants have shown significant curative effects in the treatment of various diseases. UDP-dependent glycosyltransferases in plants play a crucial role in the biosynthesis of bioactive glycosides. In this review, the characteristics, evolution, and recent advances in terpenoid UDP-glycosyltransferases (UGTs) in medicinal plants over the past 20 years are summarized, providing a reference for the study of glycosylation and biosynthetic pathways of medicinal terpenoids in plants.
JOURNAL OF EXPERIMENTAL BOTANY
(2023)
Review
Biotechnology & Applied Microbiology
Anup Adhikari, Sajan Shakya, Shreesti Shrestha, Dipa Aryal, Kavi Prasad Timalsina, Dipesh Dhakal, Yogan Khatri, Niranjan Parajuli
Summary: Cytochrome P450s are heme-binding monooxygenases that catalyze functionalization of various bonds, including heteroatom oxidation and C-C bond cleavage. Despite their biotechnological potential, P450s require redox partners for chemical transformations. This review focuses on the role of P450s in antibiotic biosynthesis, specifically macrolides, aminocoumarin antibiotics, NRPSs antibiotics, and RiPPs antibiotics.
BIOTECHNOLOGY AND BIOENGINEERING
(2023)
Review
Pharmacology & Pharmacy
Shanmin Zheng, Jiawei Guo, Fangyuan Cheng, Zhengquan Gao, Lei Du, Chunxiao Meng, Shengying Li, Xingwang Zhang
Summary: This article comprehensively reviews the progress of P450 studies in algae from 2000 to 2021. It highlights the importance of algae as photosynthetic organisms and the role of P450 enzymes in natural product biosynthesis in algae. The article also emphasizes the advancing functional studies of algal P450 enzymes with the development of algae cultivation and genetic manipulation techniques, and the potential use of photoautotrophic algae as photo-bioreactors for high-value added pharmaceutical and chemical production.
ACTA PHARMACEUTICA SINICA B
(2022)
Article
Chemistry, Multidisciplinary
Ying Huang, Sandra Hoefgen, Vito Valiante
Summary: This study elucidated the biosynthetic pathway of drimane-type sesquiterpene esters in fungi and identified key enzymes involved in the process. The findings deepen our understanding of how fungi synthesize drimane-type sesquiterpenes and corresponding esters.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2021)
Review
Chemistry, Medicinal
Erdem Carsanba, Manuela Pintado, Carla Oliveira
Summary: Terpenoids, a class of plant natural products with industrial and pharmaceutical importance, can be sustainably produced through microbial production, particularly using engineered yeast like Saccharomyces cerevisiae. Fermentation strategies play a crucial role in increasing yield and productivity of these compounds.
Review
Biochemistry & Molecular Biology
Avantika Pandey, Madhoolika Agrawal, Shashi Bhushan Agrawal
Summary: Despite a rich history and economic importance, the potential of medicinal plants has not been fully explored under different abiotic stress conditions. The penetration of UV-B radiation and contamination of heavy metals have a significant impact on the defense-related and pharmaceutically important secondary metabolites of medicinal plants, which can affect both the quality and quantity of these metabolites. Understanding the effects of UV-B and heavy metal contamination on the biosynthesis and accumulation of secondary metabolites is important for the comprehensive study of medicinal plants.
Article
Chemistry, Multidisciplinary
Qibin Chen, Guanyin Yuan, Tao Yuan, Huiting Zeng, Zheng-Rong Zou, Zong-cai Tu, Jie Gao, Yi Zou
Summary: Cytochrome P450s are versatile oxidases that catalyze important chemical transformations in the biosynthesis of natural products. We discovered a group of P450s that can reshape the cycloaraneosene framework to form a sordarinane architecture. One of these enzymes, SdnB, cleaves the C-C bond of the epoxy residue to yield formyl groups, while SdnF selectively oxidizes one of the formyl groups to a carboxyl group and accelerates the final cyclization reaction.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2022)
Review
Biotechnology & Applied Microbiology
Junping He, Lu Yao, Lorenzo Pecoraro, Changxiao Liu, Juan Wang, Luqi Huang, Wenyuan Gao
Summary: This review summarizes the mechanisms by which cold stress promotes the accumulation of flavonoids and terpenoids in plants, including the regulation of plant hormones, transcription factors, and key enzyme genes. This provides valuable insights for future research in plant genetic engineering breeding, metabolism regulation, and synthetic biology.
CRITICAL REVIEWS IN BIOTECHNOLOGY
(2023)
Review
Biotechnology & Applied Microbiology
Shuaiqi Meng, Yu Ji, LeiLei Zhu, Gaurao Dhoke, Mehdi D. Davari, Ulrich Schwaneberg
Summary: Cytochrome P450s are important enzymes widely used in pharmaceuticals and biosynthesis. Engineering them can improve coupling efficiency and reduce undesirable reactions.
BIOTECHNOLOGY ADVANCES
(2022)
Article
Chemistry, Multidisciplinary
Yan-Long Yang, Man Zhou, Lin Yang, Markus Gressler, Johannes Rassbach, Jacob M. Wurlitzer, Ying Zeng, Kun Gao, Dirk Hoffmeister
Summary: In this study, the genetic locus responsible for the biosynthesis of panepoxydone in the basidiomycete mushroom Panus rudis was identified. The cytochrome P450 enzyme PanH was characterized as the catalyst for epoxide formation in this pathway. The study also demonstrated the ability to synthesize bioactive epoxycyclohexenone compounds using a PanH-producing yeast as a biocatalyst.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2023)
Review
Pharmacology & Pharmacy
Soledad Ravetti, Ariel G. Garro, Agustina Gaitan, Mariano Murature, Mariela Galiano, Sofia G. Brignone, Santiago D. Palma
Summary: Polyphenols, including naringin, have various biological effects and potential therapeutic applications. However, naringin faces limitations such as oxidation susceptibility, poor water solubility, and enzymatic metabolism. The development of naringin nanoformulations has overcome these limitations, making it more suitable for clinical application and improving its bioactivity.
Review
Pharmacology & Pharmacy
Mohsen Akbarian, Shu-Hui Chen
Summary: Maintaining the stability of protein and peptide drugs is crucial, and various factors such as environmental conditions and storage affect their stability. Genetic engineering and solvent engineering are effective solutions to overcome these instability issues. This article discusses the types of stress that lead to instabilities in different proteins used in pharmaceuticals and reviews the methods to combat these stresses. Additionally, it provides a brief summary of analytical methods used to detect instabilities in their primary and higher order structures.
Review
Biotechnology & Applied Microbiology
Zihan Zhang, Qing-Yang Wu, Yue Ge, Zheng-Yu Huang, Ran Hong, Aitao Li, Jian-He Xu, Hui-Lei Yu
Summary: This review article highlights the research progress in the identification, molecular modification, and functional expression of hydroxylases in the past decade, which are beneficial for the discovery, engineering, and application of more hydroxylases involved in plant secondary metabolism.
BIORESOURCES AND BIOPROCESSING
(2023)
Article
Chemistry, Physical
T. Rehka, Deepti Sharma, Fu Lin, Yeu Khai Choong, Chinchin Lim, Chacko Jobichen, Congqiang Zhang
Summary: The crystal structure of Agrocybe pediades linalool synthase (Ap.LS) was determined, revealing that its specificity is determined by the region of 60-69aa and Tyr299. Mutants of Ap.LS Y299 produced long-chain products. It was also found that microbial terpene synthases with asparagine at a specific position mainly produce cyclized products, while those with a bulky tyrosine produce linear products.
Article
Plant Sciences
Minghui Chen, Tingxiang Yan, Qian Shen, Xu Lu, Qifang Pan, Youran Huang, Yueli Tang, Xueqing Fu, Meng Liu, Weimin Jiang, Zongyou Lv, Pu Shi, Ya-nan Ma, Xiaolong Hao, Lida Zhang, Ling Li, Kexuan Tang
Article
Chemistry, Multidisciplinary
Xin-Guang Liu, Xu Lu, Ji-Xin Wang, Bin Wu, Lin Lin, Hui-Ying Wang, Ru-Zhou Guo, Ping Li, Hua Yang
Article
Agricultural Engineering
Xu Lu, Qian Shen, Ling Zhang, Fangyuan Zhang, Weimin Jiang, Zongyou Lv, Tingxiang Yan, Xueqing Fu, Guofeng Wang, Kexuan Tang
INDUSTRIAL CROPS AND PRODUCTS
(2013)
Article
Biotechnology & Applied Microbiology
Tingwen Lin, Jinfa Du, Xiaoyan Zheng, Ping Zhou, Ping Li, Xu Lu
Article
Plant Sciences
Tong-Tong Jin, Feng-Jie Liu, Yan Jiang, Long Wang, Xu Lu, Ping Li, Hui-Jun Li
Summary: The new strategy of molecular-network-guided discovery can efficiently differentiate the chemical differences in medicinal herbs with close genetic relationship and identify species-specific markers. These markers can be used for quality and safety control in medicinal herb industry.
Article
Biochemical Research Methods
Meng-Ning Li, Bing-Qing Shen, Xu Lu, Wen Gao, Shan-Shan Wen, Xuan Zhang, Hua Yang, Ping Li
Summary: The study developed an integrated two-step filtering strategy using LC-IM-QTOF MS for efficient identification of analogues in herbal medicines. By utilizing power regression and mass defect filtering, the method successfully identified multiple isoquinoline alkaloids in different herbal materials, showing higher efficiencies in excluding background interference and reducing false-positive rates compared to previous approaches.
JOURNAL OF CHROMATOGRAPHY A
(2021)
Review
Biochemistry & Molecular Biology
Xin-Guang Liu, Xu Lu, Wen Gao, Ping Li, Hua Yang
Summary: Ginkgo biloba is a unique plant with compounds that broaden the chemical diversity of herbal medicine, used for cardiovascular disease prevention and treatment. However, traditional extraction methods yield low purity and content of active compounds, highlighting the need for new strategies to meet international market demand.
NATURAL PRODUCT REPORTS
(2022)
Article
Biotechnology & Applied Microbiology
Ziting Huo, Wenbo Xu, Huijun Guo, Peng Yang, Qianwen Zhang, Xu Lu, Long Wang
Summary: In this study, the complete chloroplast genome of Persicaria perfoliata was sequenced and compared with other related species, revealing its unique genomic structure and providing potential molecular markers for its accurate identification. Phylogenetic analysis showed a close relationship between P. perfoliata and Persicaria chinensis.
Review
Plant Sciences
Xu Lu, Lijin Huang, Henrik Scheller, Jay D. Keasling
Summary: Terpenoid glycosides derived from medicinal plants have shown significant curative effects in the treatment of various diseases. UDP-dependent glycosyltransferases in plants play a crucial role in the biosynthesis of bioactive glycosides. In this review, the characteristics, evolution, and recent advances in terpenoid UDP-glycosyltransferases (UGTs) in medicinal plants over the past 20 years are summarized, providing a reference for the study of glycosylation and biosynthetic pathways of medicinal terpenoids in plants.
JOURNAL OF EXPERIMENTAL BOTANY
(2023)
Article
Forestry
Jin-fa Du, Zhen Zhao, Wen-bo Xu, Qiao-lei Wang, Ping Li, Xu Lu
Summary: This study investigated the regulatory roles of the JAZ gene family and GbCOI1/GbJAZs/GbMYC2 module in ginkgolide biosynthesis in Ginkgo biloba. The results showed that these regulatory factors play an important role in the biosynthesis of ginkgolides.
Article
Plant Sciences
Fu-Gui Li, Xin-Yue Shi, Liu Yang, Xu Lu, Yan Qi, Ping Li, Hua Yang, Wen Gao
Summary: This study aims to provide a basic proteome profile and help further discover active proteins and quality control for medicinal leeches, which would also provide insight into the research of animal medicines.
JOURNAL OF ETHNOPHARMACOLOGY
(2024)
Article
Biochemistry & Molecular Biology
Long Wang, Gengyu Lu, Hao Liu, Lijin Huang, Weimin Jiang, Ping Li, Xu Lu
GENETICS AND MOLECULAR BIOLOGY
(2020)
Article
Biochemistry & Molecular Biology
Ning Wang, Long Wang, Lianwen Qi, Xu Lu
BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY
(2018)
