Article
Biochemistry & Molecular Biology
Jiaoqi Gao, Wei Yu, Yunxia Li, Mingjie Jin, Lun Yao, Yongjin J. Zhou
Summary: Engineered yeast Ogataea polymorpha has been used to co-utilize glucose and xylose for the production of free fatty acids (FFAs) and 3-hydroxypropionic acid (3-HP) from lignocellulose.
NATURE CHEMICAL BIOLOGY
(2023)
Article
Microbiology
Tobias Busche, Hana Dostalova, Lenka Rucka, Jiri Holatko, Ivan Barvik, Vaclav Stepanek, Miroslav Patek, Joern Kalinowski
Summary: The sigma H and sigma E subunits of Corynebacterium glutamicum RNA polymerase belong to Group 4 of sigma factors, also called extracytoplasmic function (ECF) sigma factors. While the genes of the C. glutamicum sigma(Eta) regulon involved in heat and oxidative stress response have been studied, the genes of the sigma(E) regulon, involved in cell surface stress response, have not been explored until now. Differential gene expression analysis revealed a significant overlap between the sigma(Eta) and sigma(E) regulons, and identified genes involved in protein quality control, regulation of Clp proteases, and membrane integrity maintenance.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Takahisa Kogure, Masako Suda, Kazumi Hiraga, Masayuki Inui
Summary: Protocatechuic acid (PCA) is a natural bioactive phenolic acid that can be produced by Corynebacterium glutamicum through the DHS and 4-HBA pathways. Simultaneous utilization of both pathways is an efficient method for PCA production, with the highest reported productivity reaching 82.7 g/L.
METABOLIC ENGINEERING
(2021)
Article
Agricultural Engineering
Qi Sheng, Xiaoyu Wu, Yan Jiang, Zhimin Li, Fei Wang, Bin Zhang
Summary: This study demonstrated the biobased production of L-ornithine from mannitol using engineered Corynebacterium glutamicum, providing a reference for the production of additional chemicals from mannitol.
BIORESOURCE TECHNOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Soong-bin Kang, Jong-il Choi
Summary: The introduction of the response regulator DR1558 successfully enhanced the production of cadaverine in C. glutamicum under acidic conditions, indicating its impact on gene expression and stress defense mechanisms.
PROCESS BIOCHEMISTRY
(2021)
Article
Biotechnology & Applied Microbiology
Sheng-Ling Chen, Ting-Shan Liu, Wei-Guo Zhang, Jian-Zhong Xu
Summary: In this study, an NADPH-auxotrophic C. glutamicum strain was constructed by inactivating NADPH-regeneration enzymes. By replacing enzymes and optimizing metabolic pathway, a high-yielding strain capable of producing L-leucine with higher efficiency was obtained.
INTERNATIONAL MICROBIOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Dominik Weixler, Max Berghoff, Kirill Ovchinnikov, Sebastian Reich, Oliver Goldbeck, Gerd M. Seibold, Christoph Wittmann, Nadav S. Bar, Bernhard J. Eikmanns, Dzung B. Diep, Christian U. Riedel
Summary: We have successfully established a two-step process for recombinant production of active nisin with C. glutamicum. This allows for the production of a bacteriocin with complex posttranslational modifications using C. glutamicum, expanding the range of bioactive compounds that can be produced. Our results provide a foundation for further research to optimize product yields, transfer production to sustainable substrates, and purify pharmaceutical grade nisin.
MICROBIAL CELL FACTORIES
(2022)
Article
Engineering, Chemical
Zaiwei Man, Huihui Cui, Jin Li, Zhiqiang Cai, Jing Guo
Summary: In this study, the overexpression of homologous TreS significantly improved trehalose production by simplifying the process. Using permeabilized cells as biocatalysts, trehalose synthesis could be achieved efficiently in a short period of time.
Article
Microbiology
Nadja A. Henke, Irene Krahn, Volker F. Wendisch
Summary: This study successfully achieved strong and overexpression of target genes by designing new expression vectors and combining two strategies, providing new possibilities for the development of biotechnology applications.
Article
Biochemistry & Molecular Biology
Qiaoyun Zhu, Qi Liu, Chaoying Yao, Yuanxing Zhang, Menghao Cai
Summary: In this study, yeast transcriptional device libraries were developed for precise synthesis of value-added chemicals from methanol. The transcriptional devices showed good activity and expression strength in a methylotrophic yeast. By selecting and optimizing the devices, the production of chemicals from methanol can be significantly increased.
NUCLEIC ACIDS RESEARCH
(2022)
Article
Biochemical Research Methods
Yanting Duan, Weiji Zhai, Weijia Liu, Xiaomei Zhang, Jin-Song Shi, Xiaojuan Zhang, Zhenghong Xu
Summary: In this research, 17 core promoters and 29 RBSs in Corynebacterium glutamicum were characterized, showing significant differences in transcriptional and translational activity. The study revealed that RBSs contribute more to gene expression than promoters, but their efficacy is influenced by gene context, indicating poor orthogonality.
ACS SYNTHETIC BIOLOGY
(2021)
Article
Agricultural Engineering
Xin-Yue Chen, Hui-Zhong Sun, Bin Qiao, Chang-Hao Miao, Zheng-Jie Hou, Shu-Jing Xu, Qiu-Man Xu, Jing-Sheng Cheng
Summary: The study found that adding exogenous L-proline, establishing a consortium with recombinant bacteria, and utilizing kitchen waste for hydrolysis can significantly increase the production of lipopeptides by Bacillus amyloliquefaciens HM618. These strategies offer new approaches to improve the production of lipopeptides in an efficient and cost-effective manner.
BIORESOURCE TECHNOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Hideo Kawaguchi, Tomohisa Hasunuma, Yasuo Ohnishi, Takashi Sazuka, Akihiko Kondo, Chiaki Ogino
Summary: The study demonstrated the importance of oxygen concentration on amino acid production, showing that reducing dissolved oxygen levels can increase the specific productivity of 3,4-AHBA. Metabolome analysis revealed significant metabolic pathway changes under oxygen limitation, while an Idh deletion mutant exhibited higher 3,4-AHBA production in glucose fed-batch culture.
MICROBIAL CELL FACTORIES
(2021)
Article
Biotechnology & Applied Microbiology
Yang Liu, Wenzhi Yang, Tao Su, Chengchuan Che, Guizhi Li, Can Chen, Meiru Si
Summary: This study revealed that CssR in C. glutamicum mediates stress response through ligand-induced conformational changes of the protein, rather than cysteine oxidation-based thiol modification. CssR negatively controls the expression of the ncgl1576-ncgl1577 operon, its structural gene, and/or redox homeostasis-related genes, showing its crucial role in stress response.
MICROBIAL CELL FACTORIES
(2021)
Review
Biochemistry & Molecular Biology
Gi Yeon Kim, Jinyoung Kim, Geunyung Park, Hyeon Jin Kim, Jina Yang, Sang Woo Seo
Summary: Corynebacterium glutamicum is a promising microorganism for industrial production of amino acids, fuels, and value-added chemicals. It has been widely used in industrial microbiology and biotechnology due to its genome sequence and manipulation techniques. This review provides an overview of genomic manipulation technologies and gene expression tools for transcription and translation control in C. glutamicum, and discusses the challenges and applicable tools for future advancements.
COMPUTATIONAL AND STRUCTURAL BIOTECHNOLOGY JOURNAL
(2023)
Article
Biotechnology & Applied Microbiology
Shuhei Hashiro, Koyu Fujiuchi, Daisuke Sugimori, Hisashi Yasueda
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2018)
Article
Biochemistry & Molecular Biology
Naoko Arashida, Kazutaka Shimbo, Takeshi Terada, Takuya Okimi, Yo Kikuchi, Shuhei Hashiro, So Umekage, Hisashi Yasueda
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
(2018)
Article
Biochemistry & Molecular Biology
Shuhei Hashiro, Hisashi Yasueda
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
(2018)
Article
Biochemistry & Molecular Biology
Takashi Kawakami, Huan Cheng, Shuhei Hashiro, Yasushi Nomura, Shinya Tsukiji, Toshiaki Furuta, Teruyuki Nagamune
Meeting Abstract
Biotechnology & Applied Microbiology
Teruyuki Nagamune, Takashi Kawakami, Shuhei Hashiro, Toshiaki Furuta, Shinya Tsukiji
JOURNAL OF BIOSCIENCE AND BIOENGINEERING
(2009)
Article
Chemistry, Multidisciplinary
Shuhei Hashiro, Shinya Tsukiji, Teruyuki Nagamune
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2009)
Article
Biotechnology & Applied Microbiology
Shuhei Hashiro, Mayu Mitsuhashi, Hisashi Yasueda
JOURNAL OF BIOSCIENCE AND BIOENGINEERING
(2019)
Article
Biotechnology & Applied Microbiology
Shuhei Hashiro, Mayu Mitsuhashi, Yasuhiko Chikami, Haruka Kawaguchi, Teruyuki Niimi, Hisashi Yasueda
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2019)
Article
Biotechnology & Applied Microbiology
Shuhei Hashiro, Yasuhiko Chikami, Haruka Kawaguchi, Alexander A. Krylov, Teruyuki Niimi, Hisashi Yasueda
Summary: RNA-based pesticides utilize RNA interference to suppress the expression of essential genes in target pests. Through the development of a cost-competitive dsRNA overproduction system using Corynebacterium glutamicum as a host bacterium, long-chain dsRNAs were efficiently produced and shown to significantly suppress larval growth of pests like the ladybird beetle. This technology has the potential to reduce production costs and find applications in various industries, including agricultural pest control.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2021)
Article
Chemistry, Multidisciplinary
Shuhei Hashiro, Hisashi Yasueda
Summary: RNA interference (RNAi)-based pesticides are a promising alternative to conventional chemical pesticides, as they are environmentally friendly and control pests through RNAi mechanisms. The effective substance, dsRNA, inhibits vital biochemical/biological processes in pests. These products can also be used for viral disease control in aquaculture.
APPLIED SCIENCES-BASEL
(2022)