Article
Biochemistry & Molecular Biology
Je Lin Sieow, Hweixian Leong Penny, Sin Yee Gun, Ling Qiao Tan, Kaibo Duan, Joe Poh Sheng Yeong, Angela Pang, Diana Lim, Han Chong Toh, Tony Kiat Hon Lim, Edgar Engleman, Olaf Rotzschke, Lai Guan Ng, Jinmao Chen, Suet Mien Tan, Siew Cheng Wong
Summary: A large number of neutrophils infiltrate tumors and contribute to the inflammatory tumor microenvironment. The metabolic status of these neutrophils and their role in tumor progression were investigated in a mouse model of pancreatic ductal adenocarcinoma (PDAC). Tumor-infiltrating neutrophils showed enhanced glycolytic activity compared to neutrophils from the bone marrow and blood of the same mouse. Deletion of hypoxia-inducible factor 1-alpha (HIF-1 alpha) in neutrophils reduced tumor burden and improved survival in the mouse model, indicating a potential target for metabolic modulation in cancer.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Oncology
Nancy T. Chee, Candace H. Carriere, Zachary Miller, Scott Welford, Shaun P. Brothers
Summary: Pancreatic ductal adenocarcinoma (PDAC) is a highly aggressive and difficult to treat cancer characterized by high levels of chronic hypoxia. The study reveals a previously unknown function of ATF4, a master regulator of the unfolded protein response pathway, in chronic hypoxia. ATF4 decreases HIF2 alpha mRNA and increases HIF1 alpha mRNA, leading to decreased cell migration but increased colony formation and cell viability in both chronic and acute hypoxia.
Article
Biotechnology & Applied Microbiology
Wenpeng Cao, Shan Lei, Zhirui Zeng, Chaolun Xiao, Baofei Sun, Peng Xie, Yumei Li, Daopeng Luo, Wenfeng Yu
Summary: This study identified TRAA2A as an important hypoxia-associated gene in pancreatic cancer, which is associated with tumor progression and metastasis. TRAA2A is regulated by hypoxia-inducible factor HIF1 alpha and promotes the proliferation and motility of pancreatic cancer cells via activation of the AKT pathway. Therefore, TRAA2A may serve as a novel molecular target for investigating the hypoxic response of pancreatic cancer cells.
Article
Chemistry, Multidisciplinary
Yue Yin, Xiaoyang Li, Haixia Ma, Jie Zhang, Di Yu, Ruifang Zhao, Shengji Yu, Guangjun Nie, Hai Wang
Summary: The research team developed a transformable hydrogel that can generate nanovaccines for up to 30 days after injection, showing promising potential for effective cancer immunotherapy.
Article
Fisheries
Zhihai Huang, Weiliang Guan, Xiaobo Wei, Renchi Chen, Xiamin Lyu, Gaohai Zheng, Linchun Mao
Summary: The study found that HIF-1 alpha can protect whiteleg shrimp hemocytes from apoptosis caused by air exposure through upregulating the expression of HSP70 and decreasing intracellular ROS production.
Article
Oncology
Xuyang Chen, Ling Zhang, Xiaoqin Wang, Libo Xu, Jicheng Sun, Yiran Liu, Xiaorui Liu, Dhan Kalvakolanu, Baofeng Guo
Summary: In this study, a graphene oxide (GO)-based nanocarrier, GO-polyethyleneimine (PEI)-polyethylene glycol (PEG)-folic acid (FA), was developed as a tool for targeting Stat3-specific shRNA to mouse hepatoma cells in vitro and in vivo. Significant tumor growth inhibition was observed after treatment with GO-PEI-PEG-FA-sh-Stat3 combined with infrared photothermal therapy. Therefore, GO-PEI-PEG-FA may serve as a novel nano-transformer for future clinical applications.
Review
Biochemistry & Molecular Biology
Thuy-Hang Nguyen, Stephanie Conotte, Alexandra Belayew, Anne-Emilie Decleves, Alexandre Legrand, Alexandra Tassin
Summary: Muscular dystrophies are genetic degenerative muscle disorders characterized by muscle wasting, often accompanied by respiratory impairments and hypoxemia. Hypoxic stress activates compensatory mechanisms, with HIF-1α playing a key role. Alterations in HIF-1α in muscle may impact the pathophysiology of muscular dystrophies.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Pharmacology & Pharmacy
Andrew Gdowski, Hamed Hayatshahi, Rafal Fudala, Rohan Joshi, Jin Liu, Jamboor K. Vishwanatha, Rohan Jeyarajah, Paul Guzik, Amalendu P. Ranjan
Summary: Pancreatic ductal adenocarcinoma (PDAC) is an aggressive malignancy with poor clinical outcomes. The dense stroma surrounding the tumor poses a challenge for drug delivery. A novel approach discussed in this study is the use of a self-polymerizing complex of gemcitabine (GEM) and hemoglobin S (HbS) to disrupt the tumor stroma and improve treatment efficacy. In an in vivo mouse model, the self-polymerization of HbS + GEM was more effective than individual agents at reducing tumor size. These findings suggest a potential clinical benefit of delivering the GEM and HbS complex via direct injection for patients with locally advanced disease.
Article
Oncology
Xiao-Lin Hu, Xia-Tong Huang, Jia-Ni Zhang, Jie Liu, Li-Jun Wen, Xin Xu, Jue-Yu Zhou
Summary: This study identified an excessively expressed lncRNA MIR210HG in cervical squamous cell carcinoma (CSCC), which is regulated by human papillomavirus (HPV) type 16 E6 and E7 via hypoxia-inducible factor 1 alpha (HIF-1 alpha). MIR210HG promotes proliferation, migration, and invasion of CSCC cells, and forms a feedback loop with HIF-1 alpha.
AMERICAN JOURNAL OF CANCER RESEARCH
(2022)
Article
Chemistry, Physical
Anja Aarva, Sami Sainio, Volker L. Deringer, Miguel A. Caro, Tomi Laurila
Summary: This study demonstrates how to identify and characterize the atomic structure of graphene materials using a combination of computational simulation of X-ray spectra and computer-aided interpretation of experimental spectra. By systematically comparing simulation results with experimental data, the study validates the closeness of simulated models to real samples, providing a new platform for insights into the structure and chemical composition of graphene-based materials.
JOURNAL OF PHYSICAL CHEMISTRY C
(2021)
Review
Pharmacology & Pharmacy
Min Ju Kim, Hyeyoun Chang, Gihoon Nam, Youngji Ko, Sun Hwa Kim, Thomas M. Roberts, Ju Hee Ryu
Summary: Pancreatic cancer, predicted to be the second leading cause of cancer-associated death by 2025, remains a lethal form of cancer with low patient survival rates. RNA interference (RNAi) has emerged as a promising approach for pancreatic cancer treatment, with the use of nanocarriers to overcome delivery challenges. Studies have shown that targeting either the tumor or tumor microenvironment using siRNA and miRNA can induce a strong anticancer effect in pancreatic cancer models.
Article
Cell Biology
Jinsheng Zhu, Li Zhao, Yadan Hu, Guoqi Cui, Ang Luo, Changlei Bao, Ying Han, Tong Zhou, Wenju Lu, Jian Wang, Stephen M. Black, Haiyang Tang
Summary: This study elucidates the importance of HIF2 alpha in the pathogenesis of pulmonary arterial hypertension, demonstrating the potential of HIF2 alpha-mediated gene sets as a diagnostic tool for distinguishing PAH patients. The study also found significant differences in the expression profiles of HIF2 alpha-mediated gene sets in lung tissues and peripheral blood mononuclear cells between control subjects and PAH patients.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Laura Bonfili, Chunmei Gong, Francesca Lombardi, Maria Grazia Cifone, Anna Maria Eleuteri
Summary: Dysbiosis contributes to Alzheimer's disease (AD) by affecting gut microbiota, and oral bacteriotherapy with SLAB51 shows potential benefits in reducing AD progression and neuroinflammation. Chronic supplementation with SLAB51 enhances cerebral expression of HIF-1α and reduces the levels of PHD2, effectively counteracting the increase of iNOS brain expression and nitric oxide plasma levels. The results demonstrate an additional mechanism through which SLAB51 exerts neuroprotective and anti-inflammatory effects in AD.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Engineering, Chemical
Farooque Ahmed Janjhi, Imamdin Chandio, Ayaz Ali Memon, Zubair Ahmed, Khalid Hussain Thebo, Azhar Ali Ayaz Pirzado, Ayaz Ali Hakro, Muzaffar Iqbal
Summary: This study prepared GO-based lamellar membranes from GO and ginger plant extractive (GE) with various thicknesses, showing excellent separation efficiency and ultrafast permeability for organic dyes, as well as high water permeance. The membranes also exhibited good separation efficiency in organic solvent, highlighting the great potential of GO/GE lamellar membranes in water purification, desalination, and other nanofiltration applications.
SEPARATION AND PURIFICATION TECHNOLOGY
(2021)
Article
Medicine, Research & Experimental
Tripti Singh, Pallabi Banerjee, Uditi, Sarita Kumari, Anita Chopra, Nagendra Singh, Imteyaz Qamar
Summary: Regucalcin regulates intracellular Ca2+ homeostasis and intracellular signaling pathways. Its expression in hypoxia is upregulated by HIF-1α through direct binding to the HRE binding motifs within the Regucalcin promoter.