期刊
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
卷 67, 期 23, 页码 6476-6486出版社
AMER CHEMICAL SOC
DOI: 10.1021/acs.jafc.9b02075
关键词
(-)-epigallocatechin gallate; glucosamine; caseins; nanoparticle; arthritis
资金
- Key R&D Program Project of Zhejiang Province, China [2019C02072]
- Fund for Distinguished Scholars of Zhejiang Agricultural and Forestry University [2014FR064]
- Key R&D Program Project of China [2017YFD0400803]
- Zhejiang Innovation discipline titled with Laboratory Animal Genetic Engineering [201604]
This work aims to improve the antiarthritic activity of (-)-epigallocatechin gallate (EGCG) and glucosamine (GA) through fabrication and optimization of casein protein nanoparticles (EGC-NPs). Optimized EGC-NPs were obtained with a EGCG/GA/casein ratio of 1:2:8 (w/w/w). The EGC-NPs gave a mean size of 186 +/- 3.5 nm and an entrapment efficiency of 86.8 +/- 2.7%, and they exhibited a greater inhibitory activity against human fibroblast-like synoviocytesosteoarthritis cells and human fibroblast-like synoviocytes-rheumatoid arthritis cells compared with that of the EGCG-GA mixture by 33.5% and 20.8%, respectively. Freeze-dried EGC-NPs stored at 25 degrees C during 12 months showed high dispersion stability. Moreover, the redispersion of the freeze-dried EGC-NPs produced almost no significant changes in their physicochemical properties and bioactivity. Rat experiments demonstrated that the antiarthritis effect of the EGC-NPs was significantly higher than that of EGCG-GA mixture, as assessed through an analysis of anti-inflammatory efficacy, radiographic images and histopathological assessments of paw joints, and immunohistochemical changes in serum cytokines. The enchanted antiarthritic activity in vivo was consistent with that in vitro. The EGC-NPs demonstrate potential as a food supplement for the treatment of arthritis.
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