Review
Agriculture, Multidisciplinary
Shilei Sun, Jiangsheng Zhou, Jihong Jiang, Yijun Dai, Miaomiao Sheng
Summary: The article discusses the environmental issues caused by the widespread use of NEOs in agriculture and the research progress on microbial remediation as a solution. The focus is on exploring the role and application of NHase in NEO degradation.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2021)
Article
Biotechnology & Applied Microbiology
Leilei Guo, Wenlong Yang, Xi Cheng, Zhixia Fan, Ximeng Chen, Feng Ge, Yijun Dai
Summary: The newly discovered bacterium Pseudaminobacter salicylatoxidans can effectively degrade acetamiprid to IM-1-2, with the rapid degradation achieved through overexpression of nitrile hydrases AnhA and AnhB. This discovery is significant for developing bioremediation agents to address acetamiprid pollution.
INTERNATIONAL BIODETERIORATION & BIODEGRADATION
(2021)
Article
Engineering, Environmental
Huoyong Jiang, Huaxiao Li, Yuhe Wang, Xuexiu Yu, Xiaoyue Chen, Yijun Dai
Summary: This study compares the NHase activities of two different E. coli strains and finds that the addition of a new gene enhances the activity of NHase. The immobilization of this engineered E. coli strain allows for the degradation of nitrile-containing insecticides commonly used in agriculture.
JOURNAL OF ENVIRONMENTAL CHEMICAL ENGINEERING
(2023)
Article
Biochemistry & Molecular Biology
Junling Guo, Julia Berdychowska, Qianpeng Lai, Yiwei Meng, Zhongyi Cheng, Lukasz Peplowski, Zhemin Zhou
Summary: A highly thermostable nitrile hydratase with improved catalytic performance was successfully constructed by modifying the substrate access tunnel. Various mutants of the enzyme were established, showing enhanced catalytic activity towards a range of nitrile substrates, indicating great potential for efficient synthesis of high-value amides.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2022)
Article
Biotechnology & Applied Microbiology
Huo-Yong Jiang, Hong-Kai Wu, Pan-Pan Yuan, Jing-Jing Guo, Li Wang, Yi-Jun Dai
Summary: A novel isolated bacterium, Pseudomonas stutzeri CGMCC 22915, was found to rapidly degrade Sulfoxaflor (SUL), a sulfoximine insecticide, into SUL-amide. This bacterium effectively facilitated SUL degradation in soil and surface water. Additionally, a nitrile hydratase with a different gene orientation, PsNHase, was discovered to transform SUL and other nitrile-containing insecticides into their respective amides. This research provides potential solutions for the bioremediation of SUL in contaminated soil and surface water.
INTERNATIONAL BIODETERIORATION & BIODEGRADATION
(2022)
Article
Biochemistry & Molecular Biology
Leyi Zhang, Shiyue Zhao, Cheng Chang, Jianan Wang, Chen Yang, Zhongyi Cheng
Summary: By redesigning the structure of a thermophilic nitrile hydratase, its catalytic activity and thermostability were improved under high acrylamide concentrations. Through structural analysis and simulations, specific amino acid residues were identified to be responsible for enhancing resistance to high acrylamide concentrations. The modified enzyme showed increased activity, stability, and acrylamide concentration compared to the wild-type enzyme.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Biochemistry & Molecular Biology
Junling Guo, Zhongyi Cheng, Julia Berdychowska, Xiaonan Zhu, Lingling Wang, Lukasz Peplowski, Zhemin Zhou
Summary: Protein fusion using a linker is crucial for protein evolution, but designing suitable linkers remains challenging. Longer linkers lead to higher stability for NHase, but excessively long linkers can impact activity and expression; alpha-helical linkers appear more suitable for NHase compared to flexible or rigid linkers.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Article
Multidisciplinary Sciences
Luis Bering, Elliott J. Craven, Stanley A. Sowerby Thomas, Sarah A. Shepherd, Jason Micklefield
Summary: Amides are essential chemical bonds in nature and commonly found in proteins, metabolites, and synthetic products. This study presents an integrated multi-catalytic system that combines nitrile hydratase enzymes with a Cu-catalysed N-arylation reaction for the synthesis of amide bonds. The method offers high substrate loading, broad applicability, and excellent functional group tolerance, enabling the efficient synthesis of natural product derivatives, drug molecules, and challenging chiral amides under environmentally friendly conditions.
NATURE COMMUNICATIONS
(2022)
Article
Chemistry, Applied
Weiguo Zuo, Guanqun Wang, Shunbin Yang, E. Dongxiao, Lin Liu, Hengyu Li, Song You, Xian Jia
Summary: The combination of aMOFs and biocatalysis allows for the efficient synthesis of (E)-2-cyanoacrylamides through a chemical-biological relay catalytic method.
ADVANCED SYNTHESIS & CATALYSIS
(2023)
Article
Engineering, Chemical
Senwen Deng, Shujing Zhu, Xinyi Zhang, Xi Sun, Xiaoqiang Ma, Erzheng Su
Summary: In this study, the recombinant production of nitrile hydratase (NHase) in Escherichia colt for 2-Amino-2,3-dimethylbutyramide (ADBA) synthesis was explored. NHase from Pseudonocardia thermophila JCM3095 was selected and its enzymatic properties were studied. After systematic optimization of fermentation conditions, an efficient recombinant whole-cell biocatalyst for ADBA synthesis was obtained.
Article
Biochemistry & Molecular Biology
Qin Qin, Chengye Tang, Jing Wu, Sheng Chen, Zhengfei Yan
Summary: In this study, an aminopeptidase derived from Streptomyces canus T20 was successfully expressed and characterized, showing high specific activity, thermal stability, and alkaline pH stability. The enzyme exhibited high specificity towards rice protein, resulting in a high yield of small rice peptides with significant debittering effect.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Article
Agricultural Engineering
Zhongshi Huang, Xinglong Wang, Ning Li, Fuqiang Song, Jingwen Zhou
Summary: In this study, a whole-cell catalysis method for efficient synthesis of NR was established using E. coli. The researchers confirmed the high catalytic activity of UshA in synthesizing NR and identified the genes involved in NR degradation. They also demonstrated the important role of UshA's signal peptide in NR transport. By introducing nitrile hydratase, they achieved the conversion of 3-cyanopyridine to NR. The efficient biosynthesis of NR in E. coli was achieved with high titers using nicotinamide and 3-cyanopyridine as substrates in a 5-L bioreactor.
BIORESOURCE TECHNOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Anja Kostelac, Leander Sutzl, Jolanta Puc, Valentina Furlanetto, Christina Divne, Dietmar Haltrich
Summary: This study reports the biochemical characterization of a pyranose oxidase (ScPOx) from Streptomyces canus. The results showed that ScPOx oxidizes monosaccharides and various C- and O-glycosides, and belongs to the same sequence space as the recently discovered FAD-dependent C-glycoside 3-oxidase.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Narges Malmir, Mohammadreza Zamani, Mostafa Motallebi, Najaf Allahyari Fard, Lukhanyo Mekuto
Summary: In this study, 24 fungal strains were tested for their ability to resist and degrade cyanide, and Trichoderma harzianum was found to have the highest efficiency in degrading cyanide at a concentration of 15 mM. Molecular analysis confirmed the involvement of several enzymes in the cyanide degradation process.
Article
Biochemistry & Molecular Biology
Zifang Zhou, Dong Ma, Zhongyi Cheng
Summary: The study successfully increased the activity and stability of NHase enzyme through sequence analysis and mutagenesis, developing a powerful process for nicotinamide production. The mutant demonstrated significantly enhanced activity, maintained stability, and showed promising potential for industrial production.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Article
Agriculture, Multidisciplinary
Tian-Qi Lu, Shi-Yun Mao, Shi-Lei Sun, Wen-Long Yang, Feng Ge, Yi-Jun Dai
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2016)
Article
Chemistry, Multidisciplinary
Shi-Lei Sun, Tian-Qi Lu, Wen-Long Yang, Jing-Jing Guo, Xue Rui, Shi-Yun Mao, Ling-Yan Zhou, Yi-Jun Dai
Article
Biotechnology & Applied Microbiology
Zhonghua Liu, Yijun Dai, Yu Huan, Zhenxing Liu, Lei Sun, Qianwen Zhou, Wenjian Zhang, Qi Sang, Hua Wei, Sheng Yuan
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2013)
Article
Biotechnology & Applied Microbiology
Guiyou Liu, Lei Sun, Siyuan Wang, Chen Chen, Ting Guo, Yin Ji, Xuesong Li, Guodong Huang, Hua Wei, Yijun Dai, Sheng Yuan
FOLIA MICROBIOLOGICA
(2011)
Article
Entomology
Zhonghua Liu, Yumin Cao, Yijun Dai, Jueping Ni, Hua Wei, Sheng Yuan
JOURNAL OF PESTICIDE SCIENCE
(2013)
Article
Agronomy
Zhonghua Liu, Yijun Dai, Guodong Huang, Yuyu Gu, Jueping Ni, Hua Wei, Sheng Yuan
PEST MANAGEMENT SCIENCE
(2011)
Article
Agriculture, Multidisciplinary
Shilei Sun, Zhixia Fan, Yunxiu Zhao, Leilei Guo, Yijun Dai
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2019)
Article
Agriculture, Multidisciplinary
Wen-Long Yang, Lei-Lei Guo, Zhi-Ling Dai, Ruo-Chen Qin, Yun-Xiu Zhao, Yi-Jun Dai
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2019)
Article
Biotechnology & Applied Microbiology
Leilei Guo, Zhiling Dai, Jingjing Guo, Wenlong Yang, Feng Ge, Yijun Dai
Article
Agriculture, Multidisciplinary
Wen-Long Yang, Zhi-Ling Dai, Xi Cheng, Ling Guo, Zhi-Xia Fan, Feng Ge, Yi-Jun Dai
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2020)
Article
Agriculture, Multidisciplinary
Yun-Xiu Zhao, Wen-Long Yang, Ling Guo, Huo-Yong Jiang, Xi Cheng, Yi-Jun Dai
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2020)
Article
Biotechnology & Applied Microbiology
Yun-Xiu Zhao, Li Wang, Ke-Xin Chen, Neng-Dang Jiang, Shi-Lei Sun, Feng Ge, Yi-Jun Dai
Summary: The novel pyridinamide insecticide Flonicamid poses potential risks to aquatic environments due to its wide application in agriculture and high solubility in water. Ensifer adhaerens CGMCC 6315 was found to efficiently transform Flonicamid into N-(4-trifluoromethylnicotinoyl) glycinamide using nitrile hydratases PnhA and CnhA, making it a promising candidate for bioremediation of Flonicamid contamination in surface water. Structural homology modeling suggested that residue beta-Glu56 may play a key role in the differential enzyme activity between PnhA and CnhA.
MICROBIAL CELL FACTORIES
(2021)
Article
Biotechnology & Applied Microbiology
Huo-Yong Jiang, Hong-Kai Wu, Pan-Pan Yuan, Jing-Jing Guo, Li Wang, Yi-Jun Dai
Summary: A novel isolated bacterium, Pseudomonas stutzeri CGMCC 22915, was found to rapidly degrade Sulfoxaflor (SUL), a sulfoximine insecticide, into SUL-amide. This bacterium effectively facilitated SUL degradation in soil and surface water. Additionally, a nitrile hydratase with a different gene orientation, PsNHase, was discovered to transform SUL and other nitrile-containing insecticides into their respective amides. This research provides potential solutions for the bioremediation of SUL in contaminated soil and surface water.
INTERNATIONAL BIODETERIORATION & BIODEGRADATION
(2022)
Article
Biotechnology & Applied Microbiology
Yun-Xiu Zhao, Ke-Xin Chen, Li Wang, Pan-Pan Yuan, Yi-Jun Dai
Summary: In this study, it was found that Pseudaminobacter salicylatoxidans CGMCC 1.17248 rapidly converted SUL into X11719474 via hydration pathway mediated by two nitrile hydratases. P. salicylatoxidans CGMCC 1.17248 showed high efficiency in degrading SUL, and cell immobilization further improved the degradation efficiency.