期刊
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
卷 67, 期 22, 页码 6248-6256出版社
AMER CHEMICAL SOC
DOI: 10.1021/acs.jafc.9b01208
关键词
corncob residue; high-solid fermentation; lignin amphoteric surfactant; cell membrane damage; adsorption
资金
- National Natural Science Foundation of China [21676109, 21878112]
- Science and Technology Program of Guangzhou [201707020025]
- Guangdong Special Support Plan [2016TX03Z298]
- Science and Technology Program of Guangdong [2017B090903003]
A lignin amphoteric surfactant and betaine could enhance the enzymatic hydrolysis of lignocellulose and recover cellulase. The effects of lignosulfonate quaternary ammonium salt (SLQA) and dodecyl dimethyl betaine (BS12) on enzymatic hydrolysis digestibility, ethanol yield, yeast cell viability, and other properties of high-solid enzymatic hydrolysis and fermentation of a corncob residue were studied in this research. The results suggested that SLQA and 1 g/L BS12 effectively improved the ethanol yield through enhancing enzymatic hydrolysis. SLQA had no significant effect on the yeast cell membrane and glucose fermentation. However, S g/L BS12 reduced the ethanol yield as a result of the fact that 5 g/L BS12 damaged the yeast cell membrane and inhibited the conversion of glucose to ethanol. Our research also suggested that 1 g/L BS12 enhanced the ethanol yield of corncob residue fermentation, which was attributed to the fact that lignin in the corncob adsorbed BS12 and decreased its concentration in solution to a safe level for the yeast.
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