Article
Plant Sciences
Vanessa Castro-Rodriguez, Thomas J. Kleist, Nicoline M. Gappel, Fatiha Atanjaoui, Sakiko Okumoto, Mackenzie Machado, Tom Denyer, Marja C. P. Timmermans, Wolf B. Frommer, Michael M. Wudick
Summary: Plants use electrical and chemical signals for systemic communication, with herbivory triggering glutamate accumulation and calcium waves to report damage and initiate defense. By expressing fluorescent glutamate sensors in Arabidopsis, researchers found that displaying the sensors externally impacted plant growth and development. The study also revealed that sequestering glutamate at the cell surface disrupts meristematic cell supply and localized signaling, affecting root growth.
Article
Chemistry, Multidisciplinary
Anna Milewska-Hendel, Weronika Gepfert, Maciej Zubko, Ewa Kurczynska
Summary: Gold nanoparticles had a negative impact on barley root development, causing morphological, histological, and ultrastructural changes.
APPLIED SCIENCES-BASEL
(2022)
Article
Plant Sciences
Drishti Mandal, Saptarshi Datta, Giridhar Raveendar, Pranab Kumar Mondal, Ronita Nag Chaudhuri
Summary: RAV1 gene plays an important role in regulating root growth. The expression of RAV1 gene is suppressed in mutant, resulting in impaired cytokinin signaling and altered auxin transport and distribution, ultimately affecting the meristem size of the root.
Article
Plant Sciences
Taras Pasternak, Ivan A. Paponov, Serhii Kondratenko
Summary: Procedures have been optimized for direct regeneration of entire plants from shoot and root protoplasts of Arabidopsis thaliana, including adjustments to culture media, detection protocols, and optimization of cell reprogramming. Cell division competence was detected through DNA replication events and high cell density, and further optimized by applying glutathione to increase sensitivity to cell cycle activation by auxin. The developed protocol allows investigation into the molecular mechanism of somatic plant cell de-differentiation.
Article
Plant Sciences
Brenda Anabel Lopez-Ruiz, Elsa H. Quezada-Rodriguez, Alma Pineyro-Nelson, Hugo Tovar, Berenice Garcia-Ponce, Maria de la Paz Sanchez, Elena R. Alvarez-Buylla, Adriana Garay-Arroyo
Summary: GWAS in 124 accessions identified 23 genes and five SNPs associated with primary root growth, shedding light on the genetic control mechanisms for root length in plants.
Article
Plant Sciences
Taras Pasternak, Stefan Kircher, Klaus Palme, Jose Manuel Perez-Perez
Summary: Root development is regulated by sucrose and light. Carbohydrate starvation during skotomorphogenesis leads to compaction of nuclei in the root apical meristem, hindering cell cycle progression and causing irreversible root differentiation. Adding carbohydrates promotes seedling growth. Transferring carbohydrate-grown seedlings from light to dark promotes cell elongation and reduces root meristem size. Carbohydrates are essential for cell cycle activity and auxin-regulated response in the root apical meristem.
Article
Multidisciplinary Sciences
Camila Goldy, Jose-Antonio Pedroza-Garcia, Natalie Breakfield, Toon Cools, Rodrigo Vena, Philip N. Benfey, Lieven De Veylder, Javier Palatnik, Ramiro E. Rodriguez
Summary: Gene expression is rapidly reconfigured during the cell cycle to carry out specific cellular functions for each phase. The use of fluorescence-activated cell sorting allowed for analysis of gene expression profile in G2/M cells in the growing root and identification of key regulatory factors. SCL28, a transcription factor, was found to play a crucial role in promoting progression through G2/M and modulating cell division plane selection.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Developmental Biology
Monica L. Garcia-Gomez, Blanca J. Reyes-Hernandez, Debee P. Sahoo, Selene Napsucialy-Mendivil, Aranza X. Quintana-Armas, Jose A. Pedroza-Garcia, Svetlana Shishkova, Hector H. Torres-Martinez, Mario A. Pacheco-Escobedo, Joseph G. Dubrovsky
Summary: A continuous transition from stem cells to transit-amplifying cells and then differentiated cells is crucial in understanding the development of multicellular organisms. In this study, the researchers focused on the root apical meristem (RAM) in Arabidopsis thaliana and found that the mutation mto2-2 affected threonine synthesis and resulted in the loss of the proliferation domain (PD) in the RAM. Mathematical modeling and molecular markers were used to demonstrate the uncoupling of PD and the transition domain (TD) in the mutant.
Article
Developmental Biology
Marta J. Laskowski, Helene C. Tiley, Yiling Fang, Anabel Epstein, Yuyang Fu, Roberto Ramos, Thomas J. Drummond, Renze Heidstra, Priyanka Bhakhri, Tobias I. Baskin, Ottoline Leyser
Summary: The developmental progression in the root is affected by the phase changes in the shoot, and PLETHORA 2 plays a significant role in this network.
Review
Plant Sciences
Jesper R. van Dijk, Mario Kranchev, Ronny Blust, Ann Cuypers, Kris Vissenberg
Summary: Human activities have led to soil pollution with metals, posing risks to human health and wildlife. Establishing science-based environmental criteria and risk management policies is crucial. Understanding how metals accumulate in and affect plants is necessary, yet research on this topic is still limited.
PLANT CELL AND ENVIRONMENT
(2022)
Article
Plant Sciences
Maike Breiden, Vilde Olsson, Patrick Bluemke, Jenia Schlegel, Karine Gustavo-Pinto, Petra Dietrich, Melinka A. Butenko, Ruediger Simon
Summary: Communication between plant cells and their biotic environment heavily relies on receptor-like kinases located on the plasma membrane. Different receptors in root meristems respond to the peptide CLE40 in various ways, influencing cell differentiation and growth inhibition. CLE40 signaling also involves changes in intracellular Ca2+ concentration, potentially playing a key role in coordinating root growth with stem cell fate decisions.
PLANT AND CELL PHYSIOLOGY
(2021)
Review
Plant Sciences
Antoine Nicolas, Patrick Laufs
Summary: Plant aerial development relies on meristem activity, with shoot apical meristem (SAM) formed in the embryo contributing to the main stem and axillary meristems (AMs) formed post-embryonically contributing to the branched structure. This review discusses recent findings on the de novo establishment of AMs and emphasizes the progressive process of stem cell formation, different from functional meristems.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Biochemistry & Molecular Biology
Naga Jyothi Pullagurla, Supritam Shome, Ranjana Yadav, Debabrata Laha
Summary: Jasmonic acid (JA) is a plant hormone that regulates various physiological processes. Inositol pyrophosphates (PP-InsPs) play a role in JA perception. The ITPK1 kinase is involved in JA-dependent root architecture development by controlling the expression of different JAZ repressor proteins.
Article
Plant Sciences
Ingrid Berenice Sanchez Carrillo, Patrick C. Hoffmann, Teura Barff, Martin Beck, Hugo Germain
Summary: This study describes a workflow for preparing cryo-ET of root protoplasts from Arabidopsis thaliana plants. The method provides a novel application for studying various characteristics of plant cells.
FRONTIERS IN PLANT SCIENCE
(2023)
Article
Cell Biology
Donato Chiatante, Antonio Montagnoli, Dalila Trupiano, Gabriella Sferra, John Bryant, Thomas L. Rost, Gabriella S. Scippa
Summary: Mechanical stress in tree roots induces the production of reaction wood and new branch roots, which help prevent anchorage failure and limb damage. The vascular cambium plays a crucial role in coordinating these responses, with intense crosstalk between meristematic cells to evaluate mechanical forces and coordinate suitable responses along the root axis. The concept of a meristematic connectome suggests that various meristems in the plant body function as a single interconnected structure to adjust the plant to its environment.
Article
Materials Science, Multidisciplinary
Tomasz Goryczka, Piotr Salwa, Maciej Zubko
Summary: The properties and shape memory effect of shape memory alloys are influenced by chemical composition and production methods. The use of high-energy ball milling combined with annealing results in the production of amorphous/nanocrystalline shape memory alloys with different crystallite sizes.
MICROSCOPY AND MICROANALYSIS
(2022)
Article
Thermodynamics
Bartlomiej Toron, Krystian Mistewicz, Marcin Jesionek, Mateusz Koziol, Maciej Zubko, Danuta Stroz
Summary: In this paper, a compressed antimony selenoiodide (SbSeI)-based hybrid piezo/triboelectric nano-generator for mechanical energy harvesting is presented for the first time. The study found that the device generated a significantly higher voltage per surface area and force unit in comparison with similar literature reported values for other piezo/triboelectric generators.
Article
Plant Sciences
Kamila Godel-Jedrychowska, Ewa Kurczynska
Summary: Plasmodesmata play a crucial role in symplasmic communication in plants, regulating cell differentiation. The study focused on describing the qualitative and quantitative analyses of PD, highlighting their importance in symplasmic communication during somatic embryogenesis. Differences in PD shape and distribution were observed between symplasmic domains within and between Col-0 and 35S:BBM somatic embryos.
FUNCTIONAL PLANT BIOLOGY
(2022)
Article
Chemistry, Physical
Karsten Glowka, Maciej Zubko, Pawel Swiec, Krystian Prusik, Magdalena Szklarska, Dariusz Chrobak, Janos L. Labar, Danuta Stroz
Summary: This study investigated the influence of the (hafnium and zirconium)/molybdenum ratio on the microstructure and properties of Ti20Ta20Nb20(ZrHf)(20)Mo--x(x) (where: x = 0, 5, 10, 15, 20 at.%) high entropy alloys in an as-cast state. The results showed that the lattice parameters of the alloys decreased with increasing molybdenum concentration up to 10%, and then increased. Scanning and transmission electron microscopy confirmed the presence of a two-phase matrix microstructure and hafnium and zirconium precipitates. Mechanical property measurements revealed a decrease in hardness and Young's modulus up to 10% of Mo content, and then an increase up to 20% of molybdenum addition. Corrosion resistance measurements confirmed the high biomedical ability of the alloys.
Article
Chemistry, Multidisciplinary
Anna Milewska-Hendel, Weronika Gepfert, Maciej Zubko, Ewa Kurczynska
Summary: Gold nanoparticles had a negative impact on barley root development, causing morphological, histological, and ultrastructural changes.
APPLIED SCIENCES-BASEL
(2022)
Article
Chemistry, Physical
Edyta Matyja, Krystian Prusik, Maciej Zubko, Pawel Swiec, Grzegorz Dercz, Jan Loskot
Summary: The crystallization kinetics and structure evolution during annealing of Ni45.5Co4.5Mn36.6In13.4 powders produced by mechanical alloying were investigated. After 70 h and 100 h of mechanical alloying, the powder consisted of a mixture of amorphous and nanocrystalline body-centered cubic (bcc) phases. Annealing above 440°C resulted in phase transformations and precipitation of different phases.
Article
Materials Science, Characterization & Testing
Jan Loskot, Daniel Jezbera, Roman Loskot, Damian Busovsky, Adrian Barylski, Karsten Glowka, Piotr Duda, Krzysztof Aniolek, Katerina Voglova, Maciej Zubko
Summary: The presented research thoroughly investigated the effects of print speed on glycol-modified polyethylene terephthalate (PETG) printed using fused filament fabrication (FFF) technology. The results showed that higher print speeds led to the formation of voids and other defects on the printed product's surface and inside. The surface profile and presence of pores were also significantly influenced by the print speed. In addition, the study found a non-monotonic relationship between polymer chain orientation and print speed using polarized Raman spectroscopy, while micromechanical properties slightly depended on the print speed.
Article
Chemistry, Physical
Karsten Glowka, Maciej Zubko, Sandra Gebura, Pawel Swiec, Krystian Prusik, Magdalena Szklarska, Danuta Stroz
Summary: The influence of hafnium/(zirconium and molybdenum) ratio on the microstructure, microhardness, and corrosion resistance of Ti20Ta20Nb20(ZrMo)(20-x)Hf-x high entropy alloys in an as-cast state was investigated. The alloys exhibited multi-phase, body-centered cubic structure as predicted by thermodynamic calculations. The increase in hafnium content was found to decrease the microhardness of the alloys. The studied alloys showed high corrosion resistance in a simulated body fluid environment.
Article
Chemistry, Physical
Mieszko Kolodziej, Jean-Marc Greneche, Sandy Auguste, Bogdan Idzikowski, Maciej Zubko, Lotfi Bessais, Zbigniew Sniadecki
Summary: Iron-based compounds with a ThMn12-type structure have the potential to bridge the gap between ferrites and high performance Nd2Fe14B magnets. The advantage lies in their composition, which contains less rare earth elements. However, the main issue is the structural stability of Fe-rich alloys with a ThMn12-type structure. Various synthesis methods and stabilizing elements have been proposed to address this issue.
Article
Materials Science, Multidisciplinary
Magdalena Laskowska, Alain Walcarius, Andrii Fedorchuk, Maciej Zubko, Lukasz Laskowski
Summary: This work presents a synthesis procedure for preparing vertically aligned mesoporous silica thin films with long nanometer-scale silver nanowires inside pores. The nanowires have a fine crystalline structure and a diameter of no more than 2 nm. The synthesis involves the use of silica nanoreactors and consists of two steps: fabricating an organically-functionalized silica thin film with regularly distributed silver ions inside vertically aligned pores, and thermally decomposing the film to form metallic crystalline nanowires. The resulting nanocomposite and initial material were characterized using electron microscopy and electrochemical techniques. The confinement provided by silica nanochannels enables the stabilization of small and ultra-thin nanowires and the formation of a hexagonal silver crystalline structure.
JOURNAL OF MATERIALS RESEARCH AND TECHNOLOGY-JMR&T
(2023)
Article
Materials Science, Multidisciplinary
Bozena Losiewicz, Agnieszka Stroz, Julian Kubisztal, Patrycja Osak, Maciej Zubko
Summary: This study focuses on finding new methods for modifying the surface of the biomedical Ti-13Zr-13Nb alloy for applications in regenerative medicine and personalized medicine. Using anodizing in 0.5% HF electrolyte at 20 V for 120 min, oxide nanotube (ONTs) layers of first-generation (1G) were successfully produced on the Ti-13Zr-13Nb alloy. The physico-chemical characterization of the obtained 1G ONTs was conducted using TEM and ATR-FTIR methods. In vitro corrosion resistance of the 1G ONTs and the comparative Ti-13Zr-13Nb substrate was analyzed through various methods such as open-circuit potential, Tafel curves, anodic polarization curves, and EIS methods.
Review
Plant Sciences
Ewa Kurczynska, Kamila Godel-Jedrychowska
Summary: Somatic embryogenesis is a valuable process in both basic research and practical applications. It allows scientists to study the mechanisms regulating the totipotency of plant cells and is an important method of plant propagation.
Article
Engineering, Civil
Beata Dubiel, Kewin Gola, Sylwia Staron, Hubert Pasiowiec, Paulina Indyka, Marta Gajewska, Maciej Zubko, Izabela Kalemba-Rec, Tomasz Moskalewicz, Slawomir Kac
Summary: This study investigates the effects of prolonged high-temperature annealing on the microstructure and hardness evolution of additively manufactured Inconel 625 components. The research identifies various strengthening and softening mechanisms and correlates them with the microstructural changes.
ARCHIVES OF CIVIL AND MECHANICAL ENGINEERING
(2023)
Article
Materials Science, Ceramics
Anna Zofia Szeremeta, Jan Macutkevic, Maciej Zubko, Seweryn Miga, Sarunas Svirskas, Irena Gruszka, Janusz Koperski, Juras Banys, Andrzej Molak
Summary: We report on the influence of Sr, Cr, Mn, and Mg doping ions on the structural and dielectric properties of barium calcium titanate (BCT) ceramics over a wide temperature and frequency range. Our findings reveal phase transitions from paraelectric to ferroelectric phases, distinguish diffused phase transition features induced by Cr and Mn ions, and highlight relaxor-like features caused by Mg ion doping. Additionally, the doping of Cr, Mn, and Mg ions affects the relaxation times of the B-sublattice ions jumping. Low-temperature ferroelectric-ferroelectric phase transitions were confirmed through dielectric and pyroelectric measurements, demonstrating the induction of non-zero net polarization by an applied electric field.
JOURNAL OF THE EUROPEAN CERAMIC SOCIETY
(2023)
Article
Chemistry, Multidisciplinary
Nikola Bednarska-Adam, Joanna Pisarska, Marta Kuwik, Tomasz Goryczka, Maciej Zubko, Wojciech A. Pisarski
Summary: In this study, germanate phosphors Li2MgGeO4:Ln(3+) (Ln = Pr, Tm) were synthesized and their luminescence properties were investigated using spectroscopy. X-ray diffraction analysis showed that ceramic compounds Li2MgGeO4 with Pr3+ and Tm3+ ions crystallized in a monoclinic crystal lattice. The luminescence properties of Pr3+ and Tm3+ ions were examined under different excitation wavelengths. The most intense blue emission band of Tm3+ overlapped well with a broad band near 500 nm, while this effect was not evident for Pr3+ ions. Based on excitation/emission spectra and decay measurements, the germanate ceramics Li2MgGeO4 doped with trivalent rare earth ions can be used as inorganic phosphors emitting orange (Pr3+) or blue (Tm3+) light.