Article
Engineering, Biomedical
Stephen W. Sawyer, Kazuyo Takeda, Alaadin Alayoubi, Eman Mirdamadi, Ahmed Zidan, Steven R. Bauer, Heba Degheidy
Summary: 3D bioprinting technology has great potential in the fields of regenerative medicine and tissue engineering. This study optimized the bioprinting protocol to successfully print constructs containing hMSCs, demonstrating their stability and bioactivity.
BIOMEDICAL MATERIALS
(2023)
Article
Engineering, Biomedical
Gyeong Min Lee, Se-jeong Kim, Eun Mi Kim, Eunhyung Kim, Sangmin Lee, Eunjin Lee, Hee Ho Park, Heungsoo Shin
Summary: A composite hydrogel incorporating epigallocatechin-gallate (EGCG)-coated nanofiber particles (EF) was developed. Compared to traditional GelMA hydrogel, this composite hydrogel showed significant improvements in cell viability, intracellular oxidative stress, and cell spreading and migration ability.
ACTA BIOMATERIALIA
(2022)
Article
Biochemistry & Molecular Biology
Fangfang Ni, Xin Luo, Zhu Zhao, Jingwang Yuan, Yuling Song, Chengzhi Liu, Min Huang, Lijuan Dong, Hujun Xie, Lei Cai, Gerui Ren, Qing Gu
Summary: In order to enhance the survival of Lactobacillus plantarum (P) during digestion and storage, probiotics were encapsulated in alginate (ALG) and alginate-gelatin (ALG-GE) hydrogel beads. The physicochemical properties of ALG-P-GE were superior to that of ALG-P. The inclusion of bacterial cells in the beads was confirmed by scanning electron microscopy (SEM). ALG-P-GE exhibited a high encapsulation efficiency of 97.7% and improved storage and thermal stability of the probiotic compared to ALG-P. The ALG-P-GE beads protected the probiotics from inactivation in simulated gastric fluid and released them in simulated intestinal fluid. The protective mechanism of ALG-GE for probiotics was further studied using fourier transform infrared spectroscopy (FTIR) and X-ray diffraction (XRD), which revealed gel formation through hydrogen bonding and electrostatic interactions between ALG and GE. In mimic beverage systems, ALG-P-GE beads effectively protected the encapsulated probiotics and improved their viability. The encapsulated probiotic showed significantly increased storage, thermal, and digestive stability, indicating the great potential of ALG-P-GE beads for the protection and delivery of probiotics in food systems.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Engineering, Biomedical
Onur Hasturk, Jordan A. Smiley, Miles Arnett, Jugal Kishore Sahoo, Cristian Staii, David L. Kaplan
Summary: The susceptibility of mammalian cells to harsh processing conditions limits their use in cell transplantation and tissue engineering. In this study, a method for fabricating Alg templated silk and silk/gelatin composite hydrogel microspheres using centrifugation-based droplet processing is demonstrated. The composite microspheres show improved structural stability and mechanical properties compared to ionically crosslinked Alg microspheres. Human mesenchymal stem and neural progenitor cells can be successfully encapsulated in these composite microspheres and protected from environmental factors.
ADVANCED HEALTHCARE MATERIALS
(2022)
Article
Chemistry, Applied
Joachim S. Kjesbu, Daria Zaytseva-Zotova, Sanna Samfors, Paul Gatenholm, Christofer Troedsson, Eric M. Thompson, Berit Lokensgard Strand
Summary: Alginate and cellulose nanofibrils (CNF) microbeads show promising applications in cell encapsulation, thanks to their high stability and biocompatibility. Crosslinking CNF with alginate improves the performance and cell viability of microbeads.
CARBOHYDRATE POLYMERS
(2022)
Review
Chemistry, Applied
Mengjie Xu, Miao Qin, Yizhu Cheng, Xiaolian Niu, Jinlong Kong, Xiumei Zhang, Di Huang, Huanan Wang
Summary: Alginate microgels can enhance cell survival, overcome immune clearance, act as three-dimensional extracellular matrix for cell growth, and serve as delivery vehicles for sustained release of therapeutic proteins and growth factors. Additionally, they have potential applications in regenerative medicine as injectable engineered scaffolds for tissue regrowth.
CARBOHYDRATE POLYMERS
(2021)
Article
Pharmacology & Pharmacy
E. C. Morais, H. T. Schroeder, C. H. Muller, S. R. Rodrigues, C. S. Souza, I. Homen de Bittencourt, J. H. Z. dos Santos
Summary: In this study, the effects of different encapsulating systems on cell viability and physiology were investigated using MIN-6 cells. The alginate/gelatin/PEG system and the alginate/chitosan matrix were found to be the most effective encapsulation systems in terms of cell viability and insulin secretion capacity.
JOURNAL OF DRUG DELIVERY SCIENCE AND TECHNOLOGY
(2021)
Article
Biophysics
Md Danish Eqbal, Farha Naaz, Kajal Sharma, Venkat Gundabala
Summary: The development of a 3D glass-PDMS hybrid device allows for complete on-chip generation of cell-laden alginate beads in the presence of electric fields, with active control of droplet size. This approach successfully encapsulated E. coli cells into alginate microbeads and demonstrated promising technology for cell encapsulation with applications in various fields.
COLLOIDS AND SURFACES B-BIOINTERFACES
(2021)
Article
Materials Science, Biomaterials
Thuy P. T. Nguyen, Fanyi Li, Brendan Hung, Vinh Xuan Truong, Helmut Thissen, John S. Forsythe, Jessica E. Frith
Summary: Microgels, microscale particles of hydrogel, are attracting increasing attention for tissue regeneration applications due to their ability to support cell-ECM and cell-cell interactions, as well as their high surface area-to-volume ratio facilitating nutrient and metabolite exchange. This article presents methods for fabricating and modifying microfluidic devices using common laboratory consumables, allowing for the production of microgels. The introduced pipet-based chip offers simplicity, tunability, and versatility, making it easy to assemble in most laboratories for the effective production of cell-laden microgels for tissue engineering applications.
ACS BIOMATERIALS SCIENCE & ENGINEERING
(2023)
Article
Biochemistry & Molecular Biology
Ilekuttige Priyan Shanura Fernando, Kirinde Gedara Isuru Sandanuwan Kirindage, Hyeong Nam Jeon, Eui Jeong Han, Arachchige Maheshika Kumari Jayasinghe, Ginnae Ahn
Summary: This study describes the purification of alginate from Sargassum horneri and the optimization of a method for formulating drug-loaded microparticles. The optimized F4 method produced hollow porous microparticles, which exhibited sustained drug release and protective effects on cells.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2022)
Article
Chemistry, Applied
Valerie Ow, Jun Jie Chang, Wai Hon Chooi, Yi Jian Boo, Rebekah P. T. Tan, Joey H. M. Wong, Bhav Harshad Parikh, Xinyi Su, Shi Yan Ng, Xian Jun Loh, Kun Xue
Summary: Hydrogels with multiple modes of crosslinking, such as the EPC-Alg hydrogel discussed in this study, offer improved control over properties like mechanical strength through the use of multiple stimuli, in this case temperature and Ca2+. These orthogonally crosslinked hydrogels are non-toxic to cells and have fully reversible crosslinks, allowing for modulation of gel strength by selectively removing one or both stimuli. The EPC-Alg hydrogel showed tunable modulation of thermal stability, stiffness, and gelation temperatures, and demonstrated feasibility in cell-based biomedical applications including cell encapsulation and cell therapy.
CARBOHYDRATE POLYMERS
(2023)
Article
Polymer Science
Sheril Amira Othman, Chin Fhong Soon, Nyuk Ling Ma, Kian Sek Tee, Gim Pao Lim, Marlia Morsin, Mohd Khairul Ahmad, Alyaa Idrees Abdulmaged, Sok Ching Cheong
Summary: This study demonstrated the successful bioprinting of a multilayer hexagon-shaped tissue model, which could be potentially useful for developing an in-vitro cervical cancer model.
Article
Biochemistry & Molecular Biology
Ezzatollah Fathi, Raheleh Farahzadi, Behnaz Valipour
Summary: The study investigated the effect of alginate-gelatin encapsulation on the differentiation potential of NK cells, revealing that encapsulation increased cell apoptosis and telomere length, as well as promoted NK cell differentiation through the secretion of cytokines.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Article
Materials Science, Multidisciplinary
Wisawat Keaswejjareansuk, Somrudee Keawmaloon, Nuttawat Sawangrat, Satit Puttipipatkhachorn, Teerapong Yata, Phornphimon Maitarad, Liyi Shi, Mattaka Khongkow, Katawut Namdee
Summary: Cell encapsulation in hydrogels, particularly alginate hydrogels, is a promising strategy for tissue engineering and cell therapy. However, the gradual degradation of alginate hydrogels in the physiological environment can negatively affect cell-cell interaction and tissue formation. This study successfully produced cell-encapsulated hydrogel microfibers with accelerated degradation to promote cell proliferation, showing potential for novel therapies in tissue regeneration.
MATERIALS TODAY COMMUNICATIONS
(2021)
Article
Materials Science, Biomaterials
Sohrab Samadian, Atiyeh Karbalaei, Mehrab Pourmadadi, Fatemeh Yazdian, Hamid Rashedi, Meisam Omidi, Samira Malmir
Summary: This study effectively differentiated mesenchymal stem cells into bone tissue using a Carbon Quantum Dots-Alginate-Gelatin nanocomposite as microcapsules and a scaffold. The unique structure of the Alginate-Gelatin nanocomposite played a key role in promoting osteogenesis of MSCs, demonstrating higher effectiveness compared to Alginate-Gelatin composites without CQD.
INTERNATIONAL JOURNAL OF POLYMERIC MATERIALS AND POLYMERIC BIOMATERIALS
(2022)
Article
Biochemistry & Molecular Biology
Wei-Han Hui, Yen-Lin Chen, Shu-Wei Chang
Summary: Crosslinking affects the mechanical behavior and tissue metabolism of collagen-based tissues. Aging and diabetes alter the crosslinking type and density, thus changing tissue properties. High-connectivity enzymatic crosslinking and advanced-glycation end-products result in uniform deformation under daily activity, while low-connectivity enzymatic crosslinking does not. High-connectivity model displays more sliding, while AGEs induce instability in structures near the binding sites.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Article
Biochemistry & Molecular Biology
Yao-Bin Ma, Xiao-Qiu Zhou, Wei-Dan Jiang, Pei Wu, Yang Liu, Shu-Wei Li, Ling Tang, Lu Zhang, Hai-Feng Mi, Lin Feng
Summary: Tea polyphenols (TPs) are an inexpensive feed additive that can protect the gill function of fish against Flavobacterium columnare (F. columnare) through various mechanisms, including suppression of oxidative stress, apoptosis, and inflammation.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Article
Biochemistry & Molecular Biology
Yan-Jia Guo, Jing-Ru Pang, Yu Zhang, Zhong-Rui Li, Xiao-Lin Zi, Hong-Min Liu, Ning Wang, Li-Juan Zhao, Ya Gao, Bo Wang, Piet Herdewijn, Cheng-Yun Jin, Ying Liu, Yi-Chao Zheng
Summary: This study identifies the conjugation of NEDD8 to LSD1 and its impact on GC cells, revealing the significance of LSD1 neddylation in GC.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Review
Biochemistry & Molecular Biology
Geum-Jae Jeong, Fazlurrahman Khan, Nazia Tabassum, Young-Mog Kim
Summary: Vibrio species are motile gram-negative bacteria commonly found in aquatic environments. They can adapt to environmental fluctuations and develop protective mechanisms against host immune systems. Different types of sigma and anti-sigma factors play important roles in regulating gene expression and virulence in Vibrio species.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Article
Biochemistry & Molecular Biology
Zahra Mohammadbagheri, Behrooz Movahedi, Shaghayegh Saeedi, Abbas Rahmati
Summary: In this study, a reusable biobased composite hydrogel was successfully synthesized and used for the removal of Tl(I) from aqueous solutions. The hydrogel exhibited high adsorption capacity and thermal stability, and the adsorption process was found to be thermodynamically spontaneous. The results demonstrated that the hydrogel can be reused multiple times without significant loss of its adsorption capacity.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Article
Biochemistry & Molecular Biology
Iqra Batool, Muhammad Imran, Ayesha Anwar, Farhan Ahmed Khan, Afrah E. Mohammed, Ashwag Shami, Hafiz Iqbal
Summary: Enzyme immobilization on ZnO/SnO2 and ZnO/SnO2/SA nanocomposites was studied, and the catalytic and biochemical characteristics of the immobilized enzyme were investigated. The results showed that ZnO/SnO2 and ZnO/SnO2/SA nanostructures are suitable carriers for enzyme immobilization.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Article
Biochemistry & Molecular Biology
Elias Mosaffa, Dhruvi Patel, Nasim Amiri Ramsheh, Rishikumar Indravadan Patel, Atanu Banerjee, Hossein Ghafuri
Summary: In this study, a unique 3D bead structure of adsorbent, composed of bacterial cellulose microfilaments reinforced chitosan adorned with melamine 2D plates, was synthesized for anionic dye removal. The adsorbent showed high specific surface area and abundant functional groups, resulting in exceptional adsorption capacity. The characteristics and optimal conditions of the adsorbent were assessed and analyzed.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Article
Biochemistry & Molecular Biology
Carolina Gismene, Jorge Enrique Hernandez Gonzalez, Marilia de Freitas Calmon, Andrey Fabricio Ziem Nascimento, Angela Rocio Nino Santisteban, Felipe Antunes Calil, Alana Della Torre da Silva, Paula Rahal, Rejane Maira Goes, Raghuvir Krishnaswamy Arni, Ricardo Barros Mariutti
Summary: Mammaliicoccus sciuri, a bacterium of clinical and veterinary relevance, expresses the virulence factor Exfoliative Toxin C (ExhC), which binds specifically to desmoglein-1 (Dsg1) and plays a role in tissue integrity. This study investigates the necrotic activity of ExhC and identifies a variant lacking necrotic potential but retaining enzymatic activity through mutagenesis experiments and crystal structure determination.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Article
Biochemistry & Molecular Biology
Bibhas K. Bhunia, Ashutosh Bandyopadhyay, Souradeep Dey, Biman B. Mandal
Summary: Intervertebral disc degeneration is a major cause of chronic low back pain and disability. Researchers have developed a bioactive silk-based hybrid hydrogel system functionalized with decellularized human Wharton's jelly extracellular matrix as bioactive cues. The hydrogels mimic the rheological and mechanical properties of the native nucleus pulposus tissue and support cell viability, proliferation, and tissue maturation.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Article
Biochemistry & Molecular Biology
Qun Zhang, Yonggan Yan, Zhao Li, Jing Du, Kai Zhang, Liguo Zhang, Ting Wang, Alberto Bianco, Shaohua Ge, Baojin Ma
Summary: A new crosslinking strategy has been devised to construct injectable sodium alginate hydrogels, which can be regulated for injectability and have great potential in biomedical applications.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Article
Biochemistry & Molecular Biology
Seong Eun Hong, Ji-Soo Lee, Hyeon Gyu Lee
Summary: The potential of multi-layer nanoemulsions for improving the cellular uptake, antioxidant activity, and in vitro bioaccessibility of alpha-tocopherol was examined. The results showed that the multi-layer nanoemulsions significantly enhanced the cellular uptake and antioxidant activity of alpha-tocopherol compared to free coumarin 6. Furthermore, the use of chitosan and dextran sulfate as wall materials in the multi-layer nanoemulsions led to even higher cellular uptake, in vitro bioaccessibility, and antioxidant activity of alpha-tocopherol.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Article
Biochemistry & Molecular Biology
Jingjing Yu, Yanhui Zhang, Ruoning Zhang, Yanxiang Gao, Like Mao
Summary: High internal phase emulsions (HIPEs) based on beeswax (BW) oleogels and octenyl succinic acid starch (OSA starch) were prepared by a simple one-step method. The results showed that oleogelation improved the stability and encapsulation capability of the HIPEs, which is significant for the development of novel healthy food.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Review
Biochemistry & Molecular Biology
Bi-Feng Yao, Xiu-Ju Luo, Jun Peng
Summary: The balanced and continuous fission and fusion of mitochondria, regulated by OPA1, are important for maintaining mitochondrial dynamics in cardiovascular disorders. Understanding the role of OPA1 in mitochondrial fusion can provide insights into the pathogenesis of cardiovascular diseases.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Review
Biochemistry & Molecular Biology
Gokul Paramasivam, Anandhavelu Sanmugam, Vishnu Vardhan Palem, Murugan Sevanan, Ananda Babu Sairam, Nachiappan Nachiappan, Buhyun Youn, Jung Sub Lee, Muthuchamy Nallal, Kang Hyun Park
Summary: Nanomaterials are being used to deliver therapeutic agents in living systems. Different nanotechnological applications such as immunosensors, immune assays, and drug delivery are being implemented using nanotechnology. Genomics plays a crucial role in selecting appropriate drugs for personalized cancer therapy. It helps in analyzing gene and protein expression, disease classification, and designing disease models. Delivering drugs to specific areas using nanoparticles is a challenging issue.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Article
Biochemistry & Molecular Biology
Jing Li, Wei Wang, Han Wu, Feng Peng, Hui Gao, Ying Guan
Summary: In this study, CMH-based CPF films were prepared and their thermal and barrier properties were successfully regulated. The tensile strength and water vapor barrier property of CPF films were significantly improved, making them promising for food packaging applications.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)