Article
Virology
Rongqun Wang, Chenchen Liu, Xiliang Jiang, Zhaoyan Tan, Hongrui Li, Shujin Xu, Shuaihu Zhang, Qiaoxia Shang, Holger B. Deising, Sven-Erik Behrens, Beilei Wu
Summary: A new partitivirus, Trichoderma harzianum partitivirus 2 (ThPV2), was discovered in a Trichoderma harzianum strain collected in China. The virus infection enhances the growth and development of the fungus and shows similar effects in inhibiting several important fungal and oomycete pathogens. Additionally, the ThPV2-infected strain shows moderately but significantly improved biocontrol activity against a specific pathogen compared to a cured variant strain.
Article
Plant Sciences
Dong-ru Kang, Yi Zhu, Shuai-lei Li, Peng-hui Ai, Muhammad Ayoub Khan, Hong-xu Ding, Ying Wang, Zi-cheng Wang
Summary: This study investigates the effect of MET1 gene silencing on gene expression profile in Chrysanthemum x morifolium 'Zijingling', revealing significant changes in differentially expressed genes related to essential plant biological processes. While a sufficient number of candidate genes associated with flowering were identified, the overall number of genes with significant differences between transgenic and control lines was relatively low. This study sheds light on the transcriptional level interference of CmMET1 on chrysanthemum growth and development, providing a basis for further research on epigenetic regulation mechanisms in flower induction and development.
PHYSIOLOGY AND MOLECULAR BIOLOGY OF PLANTS
(2021)
Article
Biochemistry & Molecular Biology
Byeong Hee Kang, Woon Ji Kim, Sreeparna Chowdhury, Chang Yeok Moon, Sehee Kang, Seong-Hoon Kim, Sung-Hwan Jo, Tae-Hwan Jun, Kyung Do Kim, Bo-Keun Ha
Summary: This study identified genes related to salt stress in cowpea by comparing germplasms with contrasting salt tolerance. The results provide useful information for the development of molecular markers in cowpea breeding programs.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Pharmacology & Pharmacy
Xiaoxiao Xue, Jiayu Yu, Cheng Li, Fang Wang, Yatao Guo, Yongwen Li, Huijuan Shi
Summary: This study aimed to explore the genes and pathways involved in the therapeutic effects of oxymatrine on psoriasis. The results showed that oxymatrine can alter the abnormal expression of certain genes and pathways in psoriasis patients, and multi-pathway and multi-target therapy can effectively treat psoriasis.
FRONTIERS IN PHARMACOLOGY
(2022)
Article
Plant Sciences
Shiqi Liu, Xuqian Peng, Xiaojing Wang, Weibing Zhuang
Summary: In this study, the effects of heavy metals cadmium and arsenic on tea plants were investigated. Transcriptomic analysis of tea roots revealed candidate genes involved in tolerance and accumulation of cadmium and arsenic. The results identified potential genes that may play important roles in enhancing tolerance to cadmium and arsenic stresses, providing potential targets for genetic engineering to improve multi-metal tolerance.
Article
Microbiology
Adel K. Madbouly, Younes M. Rashad, Mohamed I. M. Ibrahim, Nahla T. Elazab
Summary: This study investigated the application of an endophytic fungus for biodegradation and biosuppression of Aflatoxin B1 (AFB1) production by Aspergillus flavus. Among the screened endophytic fungi isolated from healthy maize plants, Trichoderma sp. showed the highest degradation potential (76.8%). It was identified as Trichoderma harzianum AYM3 and exhibited a 65% inhibition in the growth of A. flavus AYM2 in vitro. Co-culturing T. harzianum AYM3 and A. flavus AYM2 on maize grains significantly suppressed (67%) AFB1 production. The study also identified two AFB1-suppressing compounds, acetic acid and n-propyl acetate, and revealed the downregulating effects of T. harzianum AYM3 metabolites on the expression of aflP and aflS genes. The cytotoxicity assay using HepaRG cell line indicated the safety of T. harzianum AYM3 metabolites. These findings suggest that T. harzianum AYM3 can be used to suppress AFB1 production in maize grains.
Article
Plant Sciences
Liang Hu, Dabing Yang, Hongbo Wang, Xueshu Du, Yanming Zhang, Liping Niu, Bingliang Wan, Mingyuan Xia, Huaxiong Qi, Tongmin Mou, Aiqing You, Jinbo Li
Summary: Although rice is susceptible to various pests, the brown planthopper (BPH) is particularly destructive. The combination of BPH14 and BPH15 genes has been effective in improving rice defense against BPH. However, the molecular mechanisms of this resistance remain unclear. In this study, we analyzed the gene expression profiles of resistant rice lines containing BPH14, BPH15, or both, compared to the parent variety 'Wushansimiao'. We identified differentially expressed genes (DEGs) associated with defense response, phosphorylation, salt stress, oxidative stress, karrikin response, and chloroplast organization. Several candidate genes, including OsPOX8.1, were found to potentially contribute to BPH resistance. Our findings provide valuable insights for understanding the defense mechanisms of gene-pyramided insect-resistant rice lines and developing effective strategies for pest control.
FRONTIERS IN PLANT SCIENCE
(2023)
Article
Microbiology
Huifang Zhang, Ning Kong, Bin Liu, Yongfeng Yang, Caihua Li, Jinyu Qi, Yue Ma, Shida Ji, Zhihua Liu
Summary: This study investigated the biocontrol ability of Trichoderma harzianum CGMCC20739 (Tha739) against apple bitter rot caused by Colletotrichum gloeosporioides. The results showed that Tha739 effectively inhibited the growth of C. gloeosporioides and reduced its conidia production. In vivo tests demonstrated that Tha739 treatment could control apple bitter rot and maintain the nutritional quality of the fruit. These findings highlight the potential of T. harzianum Tha739 as a biocontrol agent for harvested apples.
MICROBIOLOGICAL RESEARCH
(2022)
Article
Biotechnology & Applied Microbiology
Zachary Gorman, Jianchi Chen, Adalberto A. Perez de Leon, Christopher Michael Wallis
Summary: This study analyzed the mitochondrial genome of a Trichoderma strain and compared four different assembly methods. The hybrid assembler, MaSuRCA, was found to be the most effective. By comparing the genes of the PAR3 strain, unique genes were identified, which could have important implications for the applications of Trichoderma.
Article
Veterinary Sciences
Chunhai Li, Yan Yan, Cheng Pan, Michael Adjei, Khuram Shahzad, Peng Wang, Meilan Pan, Kerui Li, Ye Wang, Wangsheng Zhao
Summary: In this study, differentially expressed circRNAs in the epididymis of yak and cattleyak were identified, with a total of 1,298 circRNAs discovered and 137 of them showing differential expression. The functions of some circRNAs were elucidated and verified using qPCR. GO enrichment analysis revealed that DE circRNAs were mainly associated with metabolic process, development process, immune system process, reproductive process, reproduction, biological adhesion and growth. COG classification analysis showed that DE circRNAs derived genes were primarily involved in replication, recombination and repair. KEGG pathway analysis suggested that DE circRNAs were mainly involved in RNA degradation. Additionally, a circRNA binding miRNA, Bta-mir-103, related to sperm activity, was also screened.
FRONTIERS IN VETERINARY SCIENCE
(2023)
Article
Microbiology
Xinhong Peng, Beilei Wu, Shuaihu Zhang, Mei Li, Xiliang Jiang
Summary: Trichoderma species are widely used biocontrol agents with antagonistic properties against various plant pathogens. Chlamydospores, produced by many fungi, have thick walls and high resistance to adverse conditions. Chlamydospores of Trichoderma spp. have better application potential and longer shelf life, but large-scale production remains difficult. By conducting a comprehensive analysis of transcriptome dynamics during chlamydospore formation, important pathways and genes involved in each stage were identified.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Camilla Martins de Oliveira, Leticia Harumi Oshiquiri, Nayane Oliveira Almeida, Andrei Stecca Steindorf, Mara Rubia da Rocha, Raphaela Castro Georg, Cirano Jose Ulhoa
Summary: This study investigated the impact of the nematode P. brachyurus on the gene expression of T. harzianum through RNA-Seq analysis. Among the predicted genes in the T. harzianum genome, 2,922 (21%) showed differential expression in the presence of P. brachyurus compared to its absence. The differentially expressed genes included those encoding Carbohydrate Active EnZymes (CAZy), MEROPS peptidases, and proteins related to secondary metabolite synthesis. Several metabolic pathways, such as aminobenzoate degradation and xenobiotic metabolism by cytochrome P450, were identified as being influenced by the presence of P. brachyurus. These findings enhance our understanding of the biocontrol potential of T. harzianum against P. bracyurus.
BIOLOGICAL CONTROL
(2023)
Review
Microbiology
Ziyang Xiao, Qinqin Zhao, Wei Li, Liwei Gao, Guodong Liu
Summary: In the control of plant diseases, biocontrol using Trichoderma harzianum and related species has shown efficient and safe results. To improve its biocontrol abilities, production of antimicrobial compounds and substances that trigger plant immunity should be increased, as well as resistance to different environmental stressors. Genetic engineering of the gene regulatory system can play a role in modulating various biological processes related to biocontrol. With advancements in fungal genetic engineering, it is expected that T. harzianum strains with enhanced biocontrol activities will be developed to support sustainable agriculture.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Multidisciplinary Sciences
Eleonora Porcu, Marie C. Sadler, Kaido Lepik, Chiara Auwerx, Andrew R. Wood, Antoine Weihs, Maroun S. Bou Sleiman, Diogo M. Ribeiro, Stefania Bandinelli, Toshiko Tanaka, Matthias Nauck, Uwe Volker, Olivier Delaneau, Andres Metspalu, Alexander Teumer, Timothy Frayling, Federico A. Santoni, Alexandre Reymond, Zoltan Kutalik
Summary: Identification of gene expression changes between healthy and diseased individuals can reveal mechanistic insights and biomarkers. The bi-directional transcriptome-wide Mendelian Randomization approach was proposed to assess causal effects between gene expression and complex traits. Comparing transcript levels between healthy and diseased individuals allows for the identification of differentially expressed genes that may be causes, consequences or mere correlates of the disease under scrutiny.
NATURE COMMUNICATIONS
(2021)
Article
Biotechnology & Applied Microbiology
Shida Ji, YiBo An, Huifang Zhang, Yucheng Wang, Zhihua Liu
Summary: Trichoderma can improve plant resistance to fungal pathogens through various means, with the mixture of TroT7 and ThaT2 significantly inhibiting the growth of several common fungal pathogens. This mixed biofertilizer also promotes lateral root growth of apple trees and soil metabolic processes, increasing the availability of nutrients in soil.
BIOLOGICAL CONTROL
(2021)
Review
Surgery
Wei-Ying Chien, Ya-Li Huang, Wen-Kuan Chiu, Yi-No Kang, Chiehfeng Chen
Summary: This study suggests that the use of tissue sealants can effectively reduce drainage volume and hematoma incidents in face-lift procedures.
FACIAL PLASTIC SURGERY & AESTHETIC MEDICINE
(2023)
Review
Surgery
Ting-Jung Lin, Ya-Li Huang, Yi-No Kang, Chiehfeng Chen
Summary: This study investigated the efficacy of stem cell conditioned medium (CM) combined with resurfacing in comparison with resurfacing alone. The results showed that stem cell CM significantly reduced wrinkles, pigmentation, and pores, and improved overall skin condition. Therefore, stem cell CM is a safe treatment that can enhance the efficacy of facial skin nonsurgical resurfacing modalities.
AESTHETIC PLASTIC SURGERY
(2023)
Article
Green & Sustainable Science & Technology
Xiaojun Deng, Yali Huang, Jing Zou, Zhangqi Zhong
Summary: Based on China's multiregional input-output (MRIO) tables from 2002 to 2015, this study quantitatively analyzes the spatial-temporal patterns and driving forces of virtual water trade (VWT) in Zhejiang province. The results show that Zhejiang transitions from a VWT supplier to a consumer in short-distance trade, with imports mainly coming from Jiangsu, Anhui, and Jiangxi, and exports focused on Shanghai. Short-distance VW exports are dominated by the secondary industry, while long-distance VW imports are dominated by the primary industry. The study also finds that GDP per capita, urbanization level, and urban population have a positive impact on VWT, while external dependence of water resources, water use efficiency, and industrial structure have a negative impact.
ENVIRONMENT DEVELOPMENT AND SUSTAINABILITY
(2023)
Article
Chemistry, Applied
Fang Luo, Lingyi Shen, Jinjuan Zhang, Yali Huang, Tengxiang Chen, Hong Xu, Qilong Zhang, Carl Redshaw, Xing Feng
Summary: A new aggregation-induced emission (AIE) probe, TPE-COOH, has been identified in this study to bind to a cyclic heptapeptide molecule, forming a new TPE-Peptide probe for fluorescence imaging of normal, cancer and cancer microsphere cells. The cell-specific targeting of this probe was verified with a novel analogue of TPAPy-1. Fluorescence imaging of cancer microspheres was achieved for the first time, allowing visualization and identification of these microspheres through changes in viscosity.
Article
Biochemistry & Molecular Biology
Hsi-Hsien Chen, Ya-Li Huang, Mei-Chieh Chen, Chih-Yin Wu, Ying-Chin Lin, Horng-Sheng Shiue, Sheng-Lun Hsu, Yu-Mei Hsueh
Summary: This study investigated the association between ADIPOQ polymorphism and CKD, and found that ADIPOQ rs182052 and rs1501299 risk genotypes may significantly modify the association between diabetes and CKD.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Engineering, Electrical & Electronic
Xucheng Zhang, Zhixu Jia, Yali Huang, Jiliang Wu, Ting Wang, Yuxian Chen, Yongsen Yu, Yuede Yang, Jinlong Xiao, Weiping Qin, Yongzhen Huang, Guanshi Qin
Summary: In this paper, intra-cavity dispersion engineering is proposed to reshape the bright dissipative Kerr solitons in a Brillouin laser cavity with anomalous GVD for generating flat-top OFCs with a high conversion efficiency. By introducing a piece of optical fiber with normal GVD into the above Brillouin fiber laser cavity for making a hybrid fiber cavity with near zero net cavity GVD, the intra-cavity waveform is broadened in time-domain due to the existence of normal GVD, which conquers the pulse compression caused by four-wave mixing and anomalous GVD, and leads to the formation of bright dissipative Kerr solitons and OFCs with a flat-top profile and a high conversion efficiency, via interlocking of up-switching waves. Furthermore, flat-top OFCs with step tunable mode spacing from GHz to THz are achieved via intra-cavity dispersion engineering.
JOURNAL OF LIGHTWAVE TECHNOLOGY
(2023)
Article
Public, Environmental & Occupational Health
Yu-Mei Hsueh, Wei-Jen Chen, Hui-Ling Lee, Ya-Li Huang, Horng-Sheng Shiue, Sheng-Lun Hsu, Hsi-Hsien Chen, Ying-Chin Lin
Summary: This study aimed to investigate the association between chronic kidney disease (CKD) and global DNA methylation marker, 5-methyl-2-deoxycytidine (5mdC, %), as well as the interaction between metal exposures and 5mdC (%) on CKD. The results suggest that 5mdC (%) may interact with plasma selenium and blood cadmium to influence the risk of CKD, and 5mdC (%) potentially mediates the associations between exposure to metals and renal function.
FRONTIERS IN PUBLIC HEALTH
(2023)
Article
Nutrition & Dietetics
Yu Wei, Na Huang, Xinyu Ye, Meng Liu, Meilian Wei, Yali Huang
Summary: This study aimed to investigate the effects and mechanisms of postbiotic of hawthorn-probiotic on loperamide modeled old KM mice. The results showed that postbiotic of hawthorn-probiotic improved intestinal movement and pathomorphology, elevated AQP3, Enac-?, and mucin-2 expression, accompanied by decreased serum TNF-a and cell apoptosis, but increased proliferation. Furthermore, it modified the gut microbiota of constipated mice, featured by upregulation of Lactobacillaceae.
FRONTIERS IN NUTRITION
(2023)
Article
Surgery
Man-Yun Li, Ya-Li Huang, Jin-Hua Chen, Yi-No Kang, Chiehfeng Chen
Summary: This study aimed to compare the effectiveness of energy-based devices in the treatment of acne scars using quartile grading scale and visual analog scale. The results showed that laser treatment was more effective in treating acne scars but was also associated with more pain.
FACIAL PLASTIC SURGERY & AESTHETIC MEDICINE
(2023)
Article
Engineering, Chemical
Yuan Liu, Zigang Liu, Jian Wang, Yang Liu, Yali Huang, Xuran Zhu, Ming Cheng, Jiayong Cao, Guangyang Liu, Yuping Wan, Jiying Wang, Xiaosheng Wu
Summary: Electrolyzed water (EW) and ultrasound (US) treatment are effective methods for reducing pesticides in fresh-cut vegetables, but their synergistic efficacy in fresh-cut potatoes has not been explored. This study investigated the effects of the combination of EW and US treatment on pesticide removal in different forms of fresh-cut potatoes and found the optimal process parameters. The research provides a simple and novel method for pesticide removal on fresh-cut potatoes and has the potential for application in vegetable processing plants.
JOURNAL OF FOOD PROCESS ENGINEERING
(2023)
Article
Chemistry, Inorganic & Nuclear
Rongjian Sa, Yanjie Zhang, Yali Huang, Yuansong Ye, Xiaodan Huang, Huihui Zeng, Diwen Liu
Summary: In this study, a comprehensive theoretical analysis is conducted on the structural parameters, dynamic stability, elastic properties, and optoelectronic characteristics of YbZn2X2 (X = N, P, As, Sb). The results show that YbZn2N2 is dynamically stable and the elastic constants meet the mechanical stability criteria. The analysis of electronic properties reveals that YbZn2X2 (X = N, Sb) and YbZn2X2 (X = P, As) are direct and indirect band gap semiconductors, respectively. YbZn2As2 exhibits an optimal band gap of approximately 1.2 eV and a high optical absorption coefficient, making it a promising candidate for optoelectronic devices.
JOURNAL OF SOLID STATE CHEMISTRY
(2023)
Article
Chemistry, Multidisciplinary
Ze-Ming Xu, Zhuoyi Hu, Yali Huang, Shu-Jin Bao, Zheng Niu, Jian-Ping Lang, Abdullah M. Al-Enizi, Ayman Nafady, Shengqian Ma
Summary: Hydrogenated nitrogen heterocyclic compounds are critical in various industries. Previous studies on the partial hydrogenation of these compounds have focused on costly and toxic precious metal catalysts. This research introduces a novel P/B type MOF-FLP catalyst achieved through a solvent-assisted linker incorporation approach, which significantly enhances catalytic hydrogenation reactions.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2023)
Article
Crystallography
Zhi-Yong Wang, Yang Liu, Jian-Ping Yuan, Qi-Long Zhang, Hong Xu, Ya-Li Huang
Summary: This compound possesses a triclinic crystal structure with lattice parameters of a = 5.0060(12) Å, b = 9.135(2) Å, and c = 11.650(3) Å, yielding a volume of 518.6(2) Å^3. It belongs to space group P (1) over bar (no. 2), and has an R-gt(F) value of 0.0474 and wR(ref)(F-2) value of 0.1270 at a temperature of 273(2) K. The disorder parameter is determined to be 78.492(7).
ZEITSCHRIFT FUR KRISTALLOGRAPHIE-NEW CRYSTAL STRUCTURES
(2023)
Article
Crystallography
Zhi-Yong Wang, Peng-Xin Qian, Xian-Jiong Yang, Qi-Long Zhang, Hong Xu, Ya-Li Huang
Summary: This compound is C8H8N6 center dot C5H9O2, monoclinic I2/alpha (no. 15) with lattice parameters a = 13.2666(17) angstrom, b = 11.0435(15) angstrom, c = 19.778(3) angstrom, beta = 92.707(10)degrees. The unit cell volume is V = 2894.4(7) angstrom(3), and it contains 8 molecules. The R-factor and weighted R-factor are R-gt(F) = 0.0528 and wR(ref)(F-2) = 0.1511, respectively.
ZEITSCHRIFT FUR KRISTALLOGRAPHIE-NEW CRYSTAL STRUCTURES
(2023)
Article
Chemistry, Multidisciplinary
Wenzhi Lv, Shuibin Shao, Junyue Zhang, Jianying Li, Yali Huang, Chun Mao
Summary: The study explores the preparation of magnetic mesoporous silica tubular (MMST) micromotors and their application in blood lead detection. The micromotor shows a dual response of fluorescence and speed to trace lead ions in blood. The dual-detection platform provides a new method for blood lead detection.
