Article
Cell Biology
Shuangyue Liu, Liping Chu, Mingzhu Xie, Lisha Ma, Hongmei An, Wen Zhang, Jihong Deng
Summary: miR-92a-3p is upregulated in cervical cancer tissues and promotes proliferation, invasion and cell cycle transition. LATS1 acts as a tumor suppressor by inhibiting TAZ, E-cadherin, vimentin and cyclin E, and can partly reverse the effects of miR-92a-3p on cancer cell behaviors.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Zhangjie Wang, Na Shen, Ziao Wang, Lei Yu, Song Yang, Yang Wang, Yu Liu, Gaohua Han, Qi Zhang
Summary: This study reveals that TRIM3 functions as a tumor suppressor in non-small cell lung cancer (NSCLC) by promoting cell death and inhibiting tumorigenesis. TRIM3 directly interacts with SLC7A11/xCT, leading to its degradation and inhibition of NSCLC development. Low TRIM3 expression is associated with a worse prognosis in NSCLC.
CELL DEATH AND DIFFERENTIATION
(2023)
Article
Cell Biology
Kailing Zhou, Yu Sun, Dan Dong, Chenghai Zhao, Wei Wang
Summary: EMP3 acts as a potential tumor suppressor in breast cancer by inhibiting cell cycle progression, DNA repair, and stem-like properties, leading to enhanced sensitivity of breast cancer cells to DNA-damaging agents.
CELL DEATH & DISEASE
(2021)
Review
Biochemistry & Molecular Biology
Da Young Lee, Jung Nyeo Chun, Minsoo Cho, Insuk So, Ju-Hong Jeon
Summary: E2F8 is a multifaceted transcription factor that is involved in various aspects of cancer development and progression. It has the ability to promote cancer cell proliferation and survival while also exhibiting tumor-suppressing activity. This review highlights the recent progress in understanding the role of E2F8 in human cancers and its implications for developing therapeutic strategies.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE
(2023)
Article
Pharmacology & Pharmacy
Mahek Sharan, Meenakshi Jha, Rishima Chandel, Saima Syeda, Runjhun Mathur, Niraj Kumar Jha, Saurabh Kumar Jha, Harsh Goel, Anju Shrivastava, Sushma Chauhan, Sudheer Pamidimarri, Abhimanyu Kumar Jha
Summary: Cervical cancer is a leading cause of mortality in women in developing countries, with hyper-methylation of tumor suppressor genes through HPV infection being a major cause. In this study, capsaicin was found to have an inhibitory effect on DNMT1 and could reverse the aberrant methylation of CADM1 and SOCS1 genes.
NAUNYN-SCHMIEDEBERGS ARCHIVES OF PHARMACOLOGY
(2023)
Review
Biochemistry & Molecular Biology
Manlio Tolomeo, Antonio Cascio
Summary: STAT3 is a complex regulator of transcription, with constitutive activation reported in many tumors but less frequent somatic mutations in cancer. Oncogenic targets of STAT3 have been identified, and inhibitors have been developed for cancer prevention and treatment. However, data suggest that STAT3 functions are multifaceted and its cellular role is determined by various signals and factors, making targeted therapies tailored to specific tumor types important.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Oncology
Rakhi Yadav, Priyanku Pradip Das, Sunil Sharma, Sounok Sengupta, Deepak Kumar, Ram Sagar
Summary: Nanomedicine offers new possibilities for the treatment of cervical cancer, with tailored delivery systems, precise targeting, combination therapies, real-time monitoring, and diverse nanocarriers. These systems allow for targeted delivery of therapeutic drugs to cancer cells, minimizing negative effects on healthy tissues. Surface modification of nanocarriers and co-delivery of multiple drugs also enhance treatment options. Combination therapies involving chemotherapy, gene therapy, immunotherapy, and photodynamic therapy have shown promising results in preclinical and clinical studies.
Review
Cell Biology
Hua-Chuan Zheng, Hang Xue, Cong-Yu Zhang
Summary: The REG4 gene plays a role in infection and inflammation by promoting macrophage polarization and killing inflammatory Escherichia coli. It is closely linked to tumorigenesis and is overexpressed in various cancers, contributing to aggressive behaviors and poor prognosis. REG4 can aggravate cellular phenotypes involved in tumorigenesis, migration, invasion, and resistance to chemotherapy and radiation. It also promotes the formation of colon stem cells by inhibiting Notch and activating Wnt. REG4 could serve as a biomarker and therapeutic target for cancer.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Biology
Li Chen, Fangfang Li, Shouyan Cao, Xia Li, Chao Zhou, Sai Han, Youzhong Zhang
Summary: This study aimed to investigate the role of RASAL2 in cervical cancer (CC). The expression of RASAL2 mRNA and protein was analyzed in CC tissues, cell lines, and normal cervical cells. The study found that down-regulation of RASAL2 was associated with advanced stage and metastasis of CC, while its over-expression inhibited proliferation and metastasis of CC cells. Silencing of RASAL2 expression increased the proliferation, invasion, and migration of CC cells.
Article
Biology
Li Chen, Fangfang Li, Shouyan Cao, Xia Li, Chao Zhou, Sai Han, Youzhong Zhang
Summary: This study investigated the roles of RASAL2 in cervical cancer (CC). The expression of RASAL2 mRNA and protein in CC tissues, CC cell lines, and normal cervical cells was examined. It was found that RASAL2 expression was significantly down-regulated in CC tissues and cell lines, and low expression was associated with advanced stage and metastasis of CC. Over-expression of RASAL2 inhibited proliferation and metastasis of CC cells, while silencing of RASAL2 expression increased the proliferation, invasion, and migration of CC cells. In conclusion, RASAL2 functions as a tumor suppressor in CC and is down-regulated in CC tissue samples and cell lines.
Article
Biochemistry & Molecular Biology
Yalan Luo, Yuyuan Li, Peng Ge, Kaina Zhang, Huanhuan Liu, Nan Jiang
Summary: QKI is found to be reduced in human cervical cancer, and its overexpression inhibits cell proliferation and promotes apoptosis. Through RNA-seq and qRT-PCR, hundreds of QKI-regulated alternative splicing events and differentially expressed genes were identified, closely related to cancer, apoptosis, and transcriptional regulation functions. In conclusion, QKI may impact the occurrence and development of cervical cancer by regulating gene expression through alternative splicing.
DNA AND CELL BIOLOGY
(2021)
Review
Cell Biology
Natalia Lourenco de Freitas, Maria Gabriela Deberaldini, Diana Gomes, Aline Renata Pavan, Angela Sousa, Jean Leandro Dos Santos, Christiane P. Soares
Summary: Epigenetic modifications, particularly histone acetylation, play a crucial role in cancer development by regulating gene expression. HDAC inhibitors show promise as a potential therapy for HPV-related cervical cancer treatment.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Review
Pathology
Ahmed S. Doghish, Mohamed A. Ali, Salah S. Elyan, Mahmoud A. Elrebehy, Hend H. Mohamed, Reda M. Mansour, Ayatallah Elgohary, Aml Ghanem, Ahmed H. I. Faraag, Nourhan M. Abdelmaksoud, Hebatallah Ahmed Mohamed Moustafa
Summary: Cervical cancer is a leading cause of cancer deaths in underdeveloped countries, with persistent infection of high-risk HPV playing a significant role in its development. MicroRNAs are small chain nucleic acids that can regulate various cellular events, including invasion, pathophysiology, angiogenesis, apoptosis, proliferation, and cell cycle phases in cervical cancer. Further research is needed to fully understand the potential of miRNAs in diagnosing and treating cervical cancer.
PATHOLOGY RESEARCH AND PRACTICE
(2023)
Article
Biochemistry & Molecular Biology
Cong-Hui Yao, Joon Seok Park, Kiran Kurmi, Song-Hua Hu, Giulia Notarangelo, Joseph Crowley, Heidi Jacobson, Sheng Hui, Arlene H. Sharpe, Marcia C. Haigis
Summary: The glycerol-3-phosphate shuttle is uncoupled in kidney cancer cells, with the cytosolic reaction being much faster than the mitochondrial reaction. Knocking down mitochondrial GPD has no effect on mitochondrial respiration, but leads to upregulation of cytosolic GPD and increased glycerol-3-phosphate supply, promoting cancer cell proliferation. Inhibition of lipid synthesis can abolish the proliferative advantage of GPD2 knockdown tumor.
Article
Oncology
Yo Han Hong, Nur Aziz, Jae Gwang Park, Dagyeong Lee, Jin Kyeong Kim, Seung A. Kim, Wooram Choi, Chae Young Lee, Hwa Pyoung Lee, Ha Thi Huyen Trang, Han Gyung Kim, Young-Jun Jeon, Bokyoung Kim, Younghoon Kim, Kyung-Hee Kim, Byong Chul Yoo, Jeung-Whan Han, Narayana Parameswaran, Ji Hye Kim, Hoon Hur, Jae Youl Cho
Summary: The study explored the pathological role of a nonhistone methyltransferase in gastric cancer (GC), identified EEF1AKMT3 as a novel tumor suppressive methyltransferase, and revealed its mechanism of action through regulating the methylation of MAP2K7 and the degradation of TP53. The findings suggest potential targeted therapy in GC by manipulating the dysregulated EEF1AKMT3/MAP2K7/TP53 signaling axis.
Article
Emergency Medicine
Aiham Qdaisat, Weixin Wu, Jun-zhong Lin, Rawan Al Soud, Zhi Yang, Zhihuang Hu, Shujun Gao, Carol C. Wu, Xiangdong Liu, Julio Silvestre, A. Guido Hita, Jayne Viets-Upchurch, Saif Al Adwan, Nafi' Al Haj Qasem, Maria T. Cruz Carreras, Kalen L. Jacobson, Patrick S. Chaftari, Hikmat Abdel-Razeq, Cielito C. Reyes-Gibby, Sai-Ching Jim Yeung
JOURNAL OF EMERGENCY MEDICINE
(2020)
Article
Obstetrics & Gynecology
Fengyi Xiao, Shujun Gao, Xindi Tang, Yisong Chen, Fang Fang
Summary: Greater awareness of OCSCUS is crucial for early detection, to alleviate patient suffering, and to avoid potential severe complications. Hysteroscopic dissection of the uterine part of the septum is a minimally invasive treatment with low surgical risk.
JOURNAL OF PEDIATRIC AND ADOLESCENT GYNECOLOGY
(2021)
Article
Oncology
Zhengfang Xu, Chengjiang Wu, Yanqiu Liu, Nian Wang, Shujun Gao, Shali Qiu, Zhutao Wang, Jing Ding, Lubin Zhang, Hui Wang, Weijiang Wu, Bing Wan, Jun Yu, Jie Fang, Peifang Yang, Qixiang Shao
Article
Biochemical Research Methods
Xiao Han, Siyi Zhong, Pengnan Zhang, Yanmei Liu, Sangsang Shi, Congquan Wu, Shujun Gao
Summary: Using iTRAQ-based mass spectrometry analysis, HMGB2 was found to be significantly up-regulated in cervical cancer tissues compared to high-grade squamous intraepithelial lesion tissues and normal cervical tissues. High HMGB2 expression was associated with primary tumor size, invasion, and tumor stage, suggesting HMGB2 as a potential prognostic biomarker and therapeutic target for cervical cancer.
CLINICAL PROTEOMICS
(2021)
Review
Cell Biology
Shujun Gao, Xiuting Liang, Hui Wang, Boyang Bao, Keyu Zhang, Yanling Zhu, Qixiang Shao
Summary: T-SCM cells have shown significant roles in cancer therapy, but excessive T-SCM cells may also contribute to autoimmune diseases and chronic HIV infection. Signaling pathways such as Wnt, AMPK/mTOR, and NF-kappa B play crucial roles in the generation, maintenance, and proinflammatory effects of T-M cells.
CELLULAR IMMUNOLOGY
(2021)
Review
Immunology
Yuefang Liu, Shujun Gao, Yangjing Zhao, Hui Wang, Qiong Pan, Qixiang Shao
Summary: This review discusses the important roles of decidual natural killer (dNK) cells in pregnancy, highlighting their generation, subsets, surface or soluble molecules, and functions crucial for maintaining pregnancy. It also explores the new functions of dNK cells, such as controlled cytotoxicity, immunosurveillance, and immunotrophism, and the molecular mechanisms involved in these functions, emphasizing their potential clinical applications.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Obstetrics & Gynecology
Yanyun Li, Ying-Xin Gong, Qing Wang, Shujun Gao, Hongwei Zhang, Feng Xie, Qing Cong, Limei Chen, Qi Zhou, Zubei Hong, Lihua Qiu, Fang Li, Yu Xie, Long Sui
Summary: This study aimed to explore the optimal method for detecting occult cervical cancers neglected by CDB&ECC, colposcopy and cytology, and found that conization may be a more effective diagnostic approach.
INTERNATIONAL JOURNAL OF WOMENS HEALTH
(2021)
Article
Oncology
Xin Wu, Hao Feng, Shujun Gao, Hua Feng, Wenjing Diao, Hongwei Zhang, Ming Du, Weihua Lou, Xipeng Wang, Tao Zhu, Yuyang Zhang, Weiguo Hu, Xiaohong Xue, Zhiling Zhu, Libing Xiang, Jiarui Li, Xuhong Fang, Yongrui Bai, Yanli Hou, Weili Yan, Ling Qiu, Hailin Yu, Shurong Zhu, Yan Du, Hua Jiang
Summary: This study aims to reevaluate the difference in survival rates between laparoscopic and abdominal radical hysterectomy in patients with early stage cervical cancer. The study will enroll 690 patients for a randomized controlled trial and follow them for at least five years. The results of this trial will provide valuable guidance for choosing appropriate treatment strategies.
Article
Medicine, Research & Experimental
Xin Wu, Ling Qiu, Weihua Lou, Xipeng Wang, Tao Zhu, Yuyang Zhang, Weiguo Hu, Xiaohong Xue, Zhiling Zhu, Libing Xiang, Jiarui Li, Xuhong Fang, Shujun Gao, Hua Feng, Wenjing Diao, Hongwei Zhang, Ming Du, Yongrui Bai, Yanli Hou, Weili Yan, Hao Feng, Hailing Yu, Shurong Zhu, Yan Du, Hua Jiang
Summary: This study aims to compare laparoscopic radical hysterectomy and abdominal radical hysterectomy in terms of survival rates among patients with early-stage cervical cancer. The results of this study are of great significance in guiding clinical practice.
Article
Microbiology
Yingying Ma, Yanpeng Li, Yanmei Liu, Le Cao, Xiao Han, Shujun Gao, Chiyu Zhang
Summary: The composition of the vaginal microbiome is associated with cervical disease, particularly cervical cancer. This study found that higher microbiome diversity and depletion of Lactobacillus species, especially L. crispatus, were associated with the severity of cervical disease. High-risk HPV16 infection also correlated with higher microbiome diversity and depletion of Lactobacillus in high-grade cervical diseases. The presence of specific bacteria in the vaginal microbiome, such as Fannyhessea vaginae, Prevotella, Bacteroides, Finegoldia, Vibrio, Veillonella, Peptostreptococcus, and Dialister, characterized the CC group.
JOURNAL OF MICROBIOLOGY
(2023)
Article
Obstetrics & Gynecology
Jialing Chen, Lin Sun, Huijun Qian, Congquan Wu, Jiqin Jiang, Xiaolan Guo, Shujun Gao
Summary: This study aimed to develop and evaluate a novel surgical procedure involving hysteroscopic fenestration with precise incision of the complete uterine septum and double cervix preservation after magnetic resonance imaging (MRI) evaluation in patients. The surgery was found to be effective in improving symptoms and achieving good reproductive outcomes.
JOURNAL OF MINIMALLY INVASIVE GYNECOLOGY
(2023)
Article
Medicine, Research & Experimental
Xiao Han, Shujuan Du, Xiaoting Chen, Xuehua Min, Zhongwei Dong, Yuyan Wang, Caixia Zhu, Fang Wei, Shujun Gao, Qiliang Cai
Summary: Lactate is associated with poor prognosis in many malignancies. This study reveals that lactate promotes cytoskeleton remodeling in cervical cancer cells, leading to increased cell adhesion and migration. The study also suggests that lactate antagonist could be a potential clinical intervention for cancer development.
Article
Virology
Yanpeng Li, Le Cao, Xiao Han, Yingying Ma, Yanmei Liu, Shujun Gao, Chiyu Zhang
Summary: In this pilot exploratory study, the vaginal eukaryotic virome in women with different levels of cervical lesions was investigated using viral metagenomics. An altered eukaryotic virome was observed in women with different levels of lesions and Lacto-bacillus profiles. Anelloviruses and papillomaviruses were the most commonly detected eukaryotic viruses of the vaginal virome, and their abundance and diversity were associated with low-grade squamous intraepithelial lesion (LSIL) and cervical cancer (CC).
Article
Nanoscience & Nanotechnology
Ashlynn L. Z. Lee, Chuan Yang, Shujun Gao, Yanming Wang, James L. Hedrick, Yi Yan Yang
ACS APPLIED MATERIALS & INTERFACES
(2020)
Article
Oncology
Pengnan Zhang, Jie Zhu, Ya Zheng, Haiyan Zhang, Hong Sun, Shujun Gao
AMERICAN JOURNAL OF TRANSLATIONAL RESEARCH
(2019)
Article
Genetics & Heredity
Xiangge Meng, Chengping Li, Yu Hei, Xiang Zhou, Guoli Zhou
Summary: The study used IVT-SAPAS sequencing to analyze the dynamic changes of APA sites during adipogenesis in bovine subcutaneous preadipocytes and intramuscular preadipocytes. The results showed that APA plays an important role in adipocyte differentiation, and UTR-APA switching genes have different trends, with intramuscular preadipocytes tending to use shorter 3'UTR for differentiation. TRIB3, WWTR1, and INSIG1 play important roles in intramuscular preadipocyte differentiation.
Article
Genetics & Heredity
S. Alizadeh, S. Khamse, N. Tajeddin, H. R. Khorram Khorshid, A. Delbari, M. Ohadi
Summary: This study identifies a specific genotype at a CG-rich trinucleotide short tandem repeat (STR) locus that is associated with late-onset neurocognitive disorders such as Alzheimer's disease (AD).
Article
Genetics & Heredity
Shougang Liu, Zhe Zhuang, Fanghua Liu, Xiuqing Yuan, Zeqiao Zhang, Xiaoqian Liang, Xinhui Li, Yongfeng Chen
Summary: This study investigated the effect and mechanism of RPL9 and TIFA in scalp psoriasis, and identified RPL9 as a potential therapeutic target for scalp psoriasis.
Article
Genetics & Heredity
Shiting Wang, Jiaqi Chen, Zhichao Jin, Ying Xing, Ruiping Wang
Summary: This study suggests a causal association between hair color and skin cancers, with light hair colors (red, blonde, and light brown) being associated with an increased risk and dark brown hair being associated with a decreased risk.
Article
Genetics & Heredity
Joseph Hawadak, Loick Pradel Kojom Foko, Rodrigue Roman Dongang Nana, Karmveer Yadav, Veena Pande, Aparup Das, Vineeta Singh
Summary: This study investigates the genetic diversity and natural selection of the Pfama-1 gene in Plasmodium falciparum isolates from Cameroon. The study finds a considerable nucleotide and haplotype diversity, as well as specific mutations in Cameroonian isolates. Positive diversifying selection and the identification of selected codon sites suggest the potential implication of these genetic variations in host immune pressure and parasite-binding complex modulation. The findings provide valuable baseline data for malaria vaccine design.
Article
Genetics & Heredity
Emily Biernat, Mansi Verma, Chhabi K. Govind
Summary: RSC is an essential ATP-dependent chromatin remodeling complex in Saccharomyces cerevisiae. It regulates nucleosome-depleted regions (NDRs) by sliding flanking nucleosomes away from NDRs. Depletion of RSC leads to nucleosome encroachment in NDRs and transcription initiation defects. The study compared the effects of catalytic-dead Sth1 and rapid depletion of Sth1 on transcription. Rapid depletion of Sth1 reduces recruitment of TBP and Pol II, while the catalytic-dead mutant exhibits a severe reduction in TBP binding but accumulates Pol II in coding regions. The results suggest a role for RSC in transcription elongation and termination processes.
Article
Genetics & Heredity
Yunxiao Wei, Yuhan Song, Muhammad Aamir Khan, Chengzhen Liang, Zhigang Meng, Yuan Wang, Sandui Guo, Rui Zhang
Summary: This study analyzed the GhTPP protein family in upland cotton for the first time and identified the important role of GhTPPA_2 in regulating sugar metabolism, improving soluble sugar accumulation, and drought stress tolerance.
Article
Genetics & Heredity
Yannan Geng, Rui Shao, Tiantong Xu, Lilong Zhang
Summary: A novel risk model based on SCUBE3, TNNC1, SPON1, SEPT12 and ULBP1 genes was developed for predicting PMOP risk, with higher risk score indicating higher risk of suffering from PMOP. Significant differences in signaling pathway activities were observed between the high-risk score group and the low-risk score group.
Article
Genetics & Heredity
Randy P. Williams, Corina Lesseur, Haoxiang Cheng, Qian Li, Maya Deyssenroth, Christopher D. Molteno, Ernesta M. Meintjes, Sandra W. Jacobson, Joseph L. Jacobson, Helen Wainwright, Ke Hao, Jia Chen, R. Colin Carter
Summary: The study suggests that heavy alcohol exposure during pregnancy may impact the proportion of fetal placental villi macrophages and increase the expression of inflammatory genes. Further research is needed to explore these effects and evaluate the potential functional roles of placental inflammation in FASD.
Article
Genetics & Heredity
Lvjing Luo, Lishuang Sun, Shu Li, Huiting Liu, Zhengyu Chen, Shi Huang, Yinyin Mo, Genliang Li
Summary: This study analyzed the expression of Ptpn1 and miR-124-3p in testicular tissues of mice and investigated their regulatory relationship. The results showed that Ptpn1 expression was up-regulated in adult mouse testis compared to juvenile mouse testis, while miR-124-3p expression showed an opposite pattern. Further analysis suggested that the down-regulation of miR-124-3p may contribute to the high expression of Ptpn1 in adult mouse testis.
Article
Genetics & Heredity
Gairui Li, Dan Zhao, Xiaolin Peng, Yashuang Zhao
Summary: MiRNA-22 shows potential as a candidate for early diagnosis of colorectal cancer (CRC). The study found higher miR-22 expression levels in the CRC and CRA groups, suggesting its potential as a biomarker for CRC early screening.
Article
Genetics & Heredity
Zhongqiu Zhu, Qianting Yang, Xiaoying Tian, Da Man, Jian Wang, Junfang Zhang, Bingshe Han
Summary: This study constructed a ceRNA network mediated by lncRNAs in cold-acclimated zebrafish ZF4 cells and revealed that upregulation of MSTRG3207 promotes apoptosis by sponging dre-miR-736 during cold acclimation.
Article
Genetics & Heredity
Weitao Chen, Denggao Xiang, Shang Gao, Shuli Zhu, Zhi Wu, Yuefei Li, Jie Li
Summary: Dam construction has negatively impacted the genetic diversity and structure of fish populations. This case study on the endangered Hemibagrus guttatus found low genetic diversity, high levels of inbreeding, and decreasing population size in fragmented populations. Genetic structure and differentiation were also observed, indicating the influence of dams on these fish populations.
Article
Genetics & Heredity
Pooja Singh, Debleena Guin, Bijay Pattnaik, Ritushree Kukreti
Summary: Through systematic literature review and meta-analysis, it was found that idiopathic pulmonary fibrosis is significantly associated with 222 polymorphisms in 118 genes. Four polymorphisms - rs35705950/MUC5B, rs2736100/TERT, rs2076295/DSP, and rs111521887/TOLLIP, exhibited substantial epidemiological evidence supporting their association with IPF risk.
Article
Genetics & Heredity
Jianping Zhang, Zhijun Cai, Fanzhe Feng, Yufeng Peng, Yi Cui, Yongiqing Xu
Summary: This study found that exosomes secreted by young BMSCs can promote the healing of tendon-bone interface after rotator cuff tears. These exosomes can improve extracellular matrix remodeling, osteogenic differentiation, angiogenesis, and stemness of tendon-derived stem cells (TDSCs). The exosomes from young BMSCs have better effects compared to those from aged BMSCs.