Article
Biotechnology & Applied Microbiology
Laura Rabbachin, Guadalupe Pinar, Irit Nir, Ariel Kushmaro, Elisabeth Eitenberger, Monika Waldherr, Alexandra Graf, Katja Sterflinger
Summary: The petroglyphs in the alpine region of Dachstein in Austria, carved in the local limestone, are important but heavily covered by biofilms. Through microscopy and metagenomic analysis, it was found that the biofilms, especially those with filamentous cyanobacteria, have a high ability to bore into the stone, causing severe disruption. Interestingly, analysis of stone without visible biofilms showed a microbiome dominated by ammonia-oxidizing archaea, indicating previous biofilm degradation and current deterioration.
INTERNATIONAL BIODETERIORATION & BIODEGRADATION
(2023)
Article
Microbiology
Joao Trovao, Fabiana Soares, Diana Sofia Paiva, Igor Tiago, Antonio Portugal
Summary: This study describes the discovery of two unknown fungi in the underground Roman crypts in Coimbra, Portugal, and proposes a new genus and species called Circumfusicillium cavernae.
Article
Microbiology
Diana S. Paiva, Luis Fernandes, Emilia Pereira, Joao Trovao, Nuno Mesquita, Igor Tiago, Antonio Portugal
Summary: In this study, the biodiversity and abundance of culturable fungi in samples from the Lemos Pantheon in Portugal were explored. The results showed a slight decrease in culturable diversity compared to previous fresh samples, with over 70% of the obtained isolates not present in the fresh samples. Potential new species were identified, and the use of a wide variety of selective culture media positively influenced the diversity of the cultivable fungi obtained. These findings highlight the importance of developing new protocols to accurately characterize the culturable fraction in a given sample and its contribution to biodeterioration.
Article
Archaeology
Joao Trovao, Fabiana Soares, Igor Tiago, Lidia Catarino, Antonio Portugal, Francisco Gil
Summary: This study evaluated the growth of different fungi on stone replicas and found that different fungi performed differently under different nutrient environments. In addition, aesthetic alterations caused by fungal growth, as well as possible erosion and mineralization phenomena of calcite layers by fungi, were observed.
JOURNAL OF CULTURAL HERITAGE
(2021)
Article
Microbiology
Joao Trovao, Fabiana Soares, Igor Tiago, Antonio Portugal
Summary: The newly proposed species Talaromyces saxoxalicus is one of the main fungi contributing to the biodeterioration of the limestone walls of the Old Cathedral of Coimbra, exhibiting an in vitro biodeteriorative ability.
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY
(2021)
Article
Microbiology
Jin Chen, Qing Zhao, Fangbing Li, Xiangwei Zhao, Yang Wang, Limin Zhang, Jinan Liu, Lingbin Yan, Lifei Yu
Summary: Microorganisms play a crucial role in rock deterioration, accelerating soil formation and ecosystem restoration. This study found that factors such as organic acid, inorganic acid, nutrient availability, and water availability affect microbial colonization of sterile limestone. The research also showed that limestone is highly receptive to microbial colonization, with rapid colonization occurring within 60 days.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Chemistry, Multidisciplinary
Diana S. Paiva, Luis Fernandes, Joao Trovao, Nuno Mesquita, Igor Tiago, Antonio Portugal
Summary: This research expands the current knowledge of the role of fungal agents in the biodeterioration of limestone rocks and provides better strategies for the conservation of cultural heritage. By analyzing the fungal community composition on a limestone-built artwork in Portugal, the study reveals the diversity of fungal communities and their relationship with different types of biodeterioration.
APPLIED SCIENCES-BASEL
(2022)
Article
Construction & Building Technology
Robert Lepage, Samuel Glass, Paul de la Bastide, Phalguni Mukhopadhyaya
Summary: This paper presents a research initiative that shifts the focus from predicting mould growth levels to probabilistic predictions, and introduces a serviceability limit state mould growth model for wood. The model's outputs were verified through an experimental roof study.
JOURNAL OF BUILDING ENGINEERING
(2022)
Article
Chemistry, Multidisciplinary
Fabiana Soares, Joao Trovao, Francisco Gil, Lidia Catarino, Igor Tiago, Antonio Portugal, Susana M. Cardoso
Summary: The study examined the effectiveness of kappa/iota carrageenans from red seaweeds against cyanobacteria proliferation on stones, demonstrating their potential to reduce colonization significantly. Marine-based products, particularly those with antimicrobial properties derived from seaweeds, were highlighted as alternative methods for biocleaning cultural heritage assets.
APPLIED SCIENCES-BASEL
(2021)
Article
Microbiology
Diana S. Paiva, Joao Trovao, Luis Fernandes, Nuno Mesquita, Igor Tiago, Antonio Portugal
Summary: Black fungi, known for their ability to survive in harsh environments, have been identified as a major contributor to the biodeterioration of stone cultural heritage materials. This study discovered a new genus and species of black fungus and evaluated its potential for deteriorating stone monuments.
Article
Biology
Amr Fouda, Mahmoud Abdel-Nasser, Ahmed M. Eid, Saad El-Din Hassan, Aya Abdel-Nasser, Nada K. Alharbi, Ahlam H. AlRokban, Gomaa Abdel-Maksoud
Summary: This study investigates the use of green synthesized titanium oxide nanoparticles to inhibit the growth of fungal strains isolated from deteriorated historical manuscripts. The nanoparticles were successfully formed using a probiotic bacterial extract and showed low toxicity against normal cells. When applied as a coating agent for leather, they effectively inhibited fungal growth and prevented further deterioration of the manuscripts.
Article
Chemistry, Multidisciplinary
Valme Jurado, Jose Luis Gonzalez-Pimentel, Bernardo Hermosin, Cesareo Saiz-Jimenez
Summary: The Salon de Reinos, a remnant of the 17th century Palacio del Buen Retiro, was built as a recreational residence under the reign of Felipe IV between 1632 and 1640 and was the main room for the monarch's receptions. This Salon owes its name to the fact that the coats of arms (shields) of the 24 kingdoms that formed Spain in Felipe IV's time were painted on the vault, above the windows. The painted ceiling and window vaults showed deterioration evidenced by fissures, water filtration, detachments of the paint layer, and black stains denoting fungal colonization related to humidity.
APPLIED SCIENCES-BASEL
(2021)
Article
Biochemistry & Molecular Biology
Viorica Maria Corbu, Irina Gheorghe, Ioana Cristina Marinas, Elisabeta Irina Geana, Maria Iasmina Moza, Ortansa Csutak, Mariana Carmen Chifiriuc
Summary: This study demonstrates the efficiency of garlic extract against over 200 biodeteriogenic microbial strains isolated from cultural heritage objects and buildings, with a particular emphasis on fungal strains. The major compounds in garlic extract include thiosulfinate, flavonoids, and polyphenols, showing inhibitory effects on planktonic growth, biofilm formation, and microbial enzyme production. This eco-friendly solution has promising potential for the conservation of tangible cultural heritage, effectively combating biodeteriogenic microorganisms.
Article
Materials Science, Multidisciplinary
Chaolong Ma, Zilong Fang, Xinjian Li, Xiaobo Liu
Summary: Bacterial communities involved in the formation of bioweathering crusts on the limestone sculptures in the Longmen Grottoes were identified, with Cyanobacteria potentially playing a major role in crust formation. The presence of various types of biogenic organic acids in the crusts suggests organic acid corrosion. Scanning electron microscopy analysis confirmed microbial penetration into the stone monuments through biomechanical attack.
Article
Biotechnology & Applied Microbiology
Yavuz Gunasdi, Ozkan Aksakal, Lokman Kemaloglu
Summary: This study aims to identify deteriorating vascular plants on historical cupolas in Erzurum and investigate their impact on the stonework. 47 plant species belonging to 19 families were identified, and it was found that Acer negundo, Scrophularia libanotica, Artemisia armeniaca, Medicago sativa, and Melilotus officinalis were the most damaging plants. Various methods to eradicate these plants were discussed. The results emphasize the importance of management strategies to remove vascular plants and the influence of building material and plant life form on degradation.
INTERNATIONAL BIODETERIORATION & BIODEGRADATION
(2023)
Article
Microbiology
A. I. Zueva, A. V. Kachalkin, I. A. Maksimova, Nguyen Van Thinh, A. V. Tiunov
Summary: Yeast populations associated with termitaria of five termite species in Cat Tien National Park in south Vietnam were investigated. The yeasts isolated from termite guts and body surface exhibited low species diversity but a relatively broad spectrum of morphological and physiological properties. The most common properties included mycelial or pseudomycelial growth, fermenting glucose, and using plant glycosides and wood degradation products as growth substrates.
Article
Ecology
D. A. Abdullabekova, E. S. Magomedova, G. G. Magomedov, A. V. Kachalkin
Summary: The study of light chestnut soil in a semi-desert-steppe area revealed it to be nonsaline, rich in bases, but lacking in certain plant nutrients. Analysis of the yeast taxonomy showed the soil's ability to store and distribute various physiological groups of yeasts in the natural cycle, expanding the potential for fungal participation in soil processes.
Article
Agriculture, Multidisciplinary
Tatiana A. Kuznetsova, Maksim V. Vecherskii, David R. Khayrullin, Aleksandr A. Stepankov, Irina A. Maximova, Aleksey Kachalkin, Nina A. Ushakova
Summary: Black soldier fly larvae (BSFL) show promise in producing cheap and high-quality dietary protein. This study investigated the fungal community of compost produced by BSFL larvae reared on a food waste substrate. The final mycobiome of the compost consisted of only two yeast species, with a total abundance of 1.2 x 10(7) CFU g(-1), after the incubation of larvae.
JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
(2022)
Article
Mycology
P. W. Crous, E. R. Osieck, Z. Jurjevi, J. Boers, A. L. Van Iperen, M. Starink-Willemse, B. Dima, S. Balashov, T. S. Bulgakov, P. R. Johnston, O. Morozova, U. Pinruan, S. Sommai, P. Alvarado, C. A. Decock, T. Lebel, S. McMullan-Fisher, G. Moreno, R. G. Shivas, L. Zhao, J. Abdollahzadeh, M. Abrinbana, D. Ageev, G. Akhmetova, A. Alexandrova, A. Altes, A. G. G. Amaral, C. Angelini, V Antonin, F. Arenas, P. Asselman, F. Badali, A. Baghela, A. Banares, R. W. Barreto, I. G. Baseia, J-M Bellanger, A. Berraf-Tebbal, A. Yu Biketova, N. Bukharova, T. Burgess, J. Cabero, M. P. S. Camara, J. F. Cano-Lira, P. Ceryngier, R. Chavez, D. A. Cowan, A. F. de Lima, R. L. Oliveira, S. Denman, Q. N. Dang, F. Dovana, I. G. Duarte, A. Eichmeier, A. Erhard, F. Esteve-Raventos, A. Fellin, G. Ferisin, R. J. Ferreira, A. Ferrer, P. Finy, E. Gaya, A. D. W. Geering, C. Gil-Duran, K. Glaessnerova, A. M. Glushakova, D. Gramaje, F. E. Guard, A. L. Guarnizo, D. Haelewaters, R. E. Halling, R. Hill, Y. Hirooka, V Hubka, V. A. Iliushin, D. D. Ivanova, N. E. Ivanushkina, P. Jangsantear, A. Justo, A. Kachalkin, S. Kato, P. Khamsuntorn, I. Y. Kirtsideli, D. G. Knapp, G. A. Kochkina, O. Koukol, G. M. Kovacs, J. Kruse, T. K. A. Kumar, I Kusan, T. Laessoe, E. Larsson, R. Lebeuf, G. Levican, M. Loizides, P. Marinho, J. J. Luangsa-ard, E. G. Lukina, V Magana-Duenas, G. Maggs-Koelling, E. F. Malysheva, V. F. Malysheva, B. Martin, M. P. Martin, N. Matocec, A. R. McTaggart, M. Mehrabi-Koushki, A. Mesic, A. N. Miller, P. Mironova, P-A Moreau, A. Morte, K. Mueller, L. G. Nagy, S. Nanu, A. Navarro-Rodenas, W. J. Nel, T. H. Nguyen, T. F. Nobrega, M. E. Noordeloos, I Olariaga, B. E. Overton, S. M. Ozerskaya, P. Palani, F. Pancorbo, V Papp, J. Pawlowska, T. Q. Pham, C. Phosri, E. S. Popov, A. Portugal, A. Posta, K. Reschke, M. Reul, G. M. Ricci, A. Rodriguez, J. Romanowski, N. Ruchikachorn, I Saar, A. Safi, B. Sakolrak, F. Salzmann, M. Sandoval-Denis, E. Sangwichein, L. Sanhueza, T. Sato, A. Sastoque, B. Senn-Irlet, A. Shibata, K. Siepe, S. Somrithipol, M. Spetik, P. Sridhar, A. M. Stchigel, K. Stuskova, N. Suwannasai, Y. P. Tan, R. Thangavel, I Tiago, S. Tiwari, Z. Tkalcec, M. A. Tomashevskaya, C. Tonegawa, H. X. Tran, N. T. Tran, J. Trovao, V. E. Trubitsyn, J. Van Wyk, W. A. S. Vieira, J. Vila, C. M. Visagie, A. Vizzini, S. V. Volobuev, D. T. Vu, N. Wangsawat, T. Yaguchi, E. Ercole, B. W. Ferreira, A. P. de Souza, B. S. Vieira, J. Z. Groenewald
Summary: This study describes novel species of fungi from various countries, found in different ecological habitats such as soil, wood, dead branches, coastal sea sand, and plant remains. The morphological and cultural characteristics of these fungi are supported by DNA barcodes.
Article
Materials Science, Biomaterials
Elena A. Deshevaya, Aleksey Kachalkin, Irina A. Maksimova, Svetlana Fialkina, Elena Shubralova, Oleg S. Tsygankov, Vyacheslav K. Ilyin, Oleg Orlov, Sergey P. Morzunov, Irina Nikolaeva
Summary: Microbiological investigation of the cloth exposed on the International Space Station (ISS) for 8 years revealed viable Rhodotorula mucilaginosa yeasts. These yeasts showed similarities to strains isolated from the ISS interior, and physiological testing indicated some differences between the cloth strain and the cultured yeast strain previously detected in the ISS interior. The investigation also demonstrated the changing sensitivity of fungal strains to antifungal drugs depending on the number of passages.
Article
Microbiology
A. V. Kachalkin
Summary: The type strain Rhodotorula pinalis VKM Y-2963 was genetically and phylogenetically characterized, and it was found to be conspecific with strains from Sphagnum mosses and dead conifer needles. Phylogenetic analysis suggests that the species Rh. pinalis should be reassigned to the genus Fellozyma.
Article
Ecology
Anna M. Glushakova, Aleksey V. Kachalkin, Aminat B. Umarova, Anna E. Ivanova, Tatiana V. Prokof'eva
Summary: A study conducted in southern cities of Russia and the Republic of Crimea in 2020 examined the presence of yeasts in soil samples from streets, urban parks, and suburban areas. The study compared the results with a previous study conducted in 2019 and found that the yeast abundance was slightly higher in 2020 after the reduction in household waste deposition. The composition of yeast communities also changed, with an increase in autochthonous basidiomycetous yeast species and a decrease in clinically significant yeasts.
Article
Microbiology
A. V. Kachalkin, A. M. Glushakova, M. A. Tomashevskaya
Summary: Leucosporidium egoroviorum f.a., sp. nov., a new anamorphic species of the basidiomycetous yeasts, was isolated as an endophyte from the fruits of Cucurbita pepo subsp. pepo (zucchini). The new species showed significant differences in genetic, physiological, and morphological characteristics compared to closely related species L. fellii, L. intermedium, and L. krtinense. The holotype KBP Y-6804T is preserved in a metabolically inactive state, and the isotype strains are VKM Y-3065, DSM 113574, and CBS 17590. The MycoBank number is MB 842805.
Article
Biochemistry & Molecular Biology
Neza Cadez, Kyria Boundy-Mills, Alfred Botha, Aleksey Kachalkin, Denes Dlauchy, Gabor Peter
Summary: During independent studies, seven yeast strains were isolated from various sources and identified as two new closely related species belonging to a new genus. Genomic sequencing, phylogenetic analysis, and physiological characterization were conducted to support their taxonomic placement and delimitation.
Article
Biotechnology & Applied Microbiology
Anna Glushakova, Aleksey Kachalkin
Summary: The study investigated yeast complexes in urban areas by studying mines on tree leaves and undamaged leaves. It was found that the abundance of yeasts increased significantly during the development of mines, and no significant differences were observed between mines formed by different insects on different trees. Twelve yeast species were observed, with ascomycetous yeasts Hanseniaspora uvarum and H. occidentalis dominating the mines.
INTERNATIONAL MICROBIOLOGY
(2023)
Article
Biology
Aleksey V. Kachalkin, Anna A. Lepeshko, Rostislav A. Streletskii, Anna M. Glushakova
Summary: This study evaluated the phytohormonal activity of Metschnikowia pulcherrima yeast strains isolated from the internal tissues of fruits. The production of IAA, zeatin, and GA3 was determined using chromatographic methods. The results showed that the strains had the highest activity in IAA synthesis, while the levels of zeatin and GA3 were much lower. These findings suggest that M. pulcherrima yeast strains may have a role in promoting plant development and growth.
Article
Biotechnology & Applied Microbiology
Anna Glushakova, Aleksey Kachalkin
Summary: Yeast complexes in fecal samples of wild and partially synanthropic birds were studied. A total of 18 yeast species were identified, with Candida species exhibiting resistance to antifungal drugs in partially synanthropic birds.
INTERNATIONAL MICROBIOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Aleksey Kachalkin, Anna Glushakova, Rostislav Streletskii
Summary: This study reports the diversity of cultivable endophytic yeasts from agricultural fruits that respond positively to the plant-promoting property of indole-3-acetic acid (IAA) production. The most active endophytic yeast strains belonged to several species. The results suggest that the internal tissues of fruits may be a promising source for the isolation of plant-beneficial yeasts that can be used to promote plant growth.
Article
Mycology
P. W. Crous, D. A. Cowan, G. Maggs-Kolling, N. Yilmaz, R. Thangavel, M. J. Wingfield, M. E. Noordeloos, B. Dima, T. E. Brandrud, G. M. Jansen, O. Morozova, J. Vila, R. G. Shivas, Y. P. Tan, S. Bishop-Hurley, E. Lacey, T. S. Marney, E. Larsson, G. Le Floch, L. Lombard, P. Nodet, V Hubka, P. Alvarado, A. Berraf-Tebbal, J. D. Reyes, G. Delgado, A. Eichmeier, J. B. Jordal, A. Kachalkin, A. Kubatova, J. G. Macia-Vicente, E. F. Malysheva, V Papp, K. C. Rajeshkumar, A. Sharma, A. Sharma, M. Spetik, D. Szaboova, M. A. Tomashevskaya, J. A. Abad, Z. G. Abad, A. Alexandrova, G. Anand, F. Arenas, N. Ashtekar, S. Balashov, A. Banares, R. Baroncelli, I Bera, A. Yu Biketova, C. L. Blomquist, T. Boekhout, D. Boertmann, T. M. Bulyonkova, T. Burgess, A. J. Carnegie, J. F. Cobo-Diaz, G. Corriol, J. H. Cunnington, M. O. da Cruz, U. Damm, N. Davoodian, A. L. C. M. de A. Santiago, J. Dearnaley, L. W. S. de Freitas, K. Dhileepan, R. Dimitrov, S. Di Piazza, S. Fatima, F. Fuljer, H. Galera, A. Ghosh, A. Giraldo, A. M. Glushakova, M. Gorczak, D. E. Gouliamova, D. Gramaje, M. Groenewald, C. K. Gunsch, A. Gutierrez, D. Holdom, J. Houbraken, A. B. Ismailov, L. Istel, T. Iturriaga, M. Jeppson, Z. Jurjevic, L. B. Kalinina, V. Kapitonov, I Kautmanova, A. N. Khalid, M. Kiran, L. Kiss, A. Kovacs, D. Kurose, I Kusan, S. Lad, T. Laessoe, H. B. Lee, J. J. Luangsa-ard, M. Lynch, A. E. Mahamedi, V. F. Malysheva, A. Mateos, N. Matocec, A. Mesic, A. N. Miller, S. Mongkolsamrit, G. Moreno, A. Morte, R. Mostowfizadeh-Ghalamfarsa, A. Naseer, A. Navarro-Rodenas, T. T. T. Nguyen, W. Noisripoom, J. E. Ntandu, J. Nuytinck, V Ostry, T. A. Pankratov, J. Pecenka, T. H. G. Pham, A. Polhorsky, A. Posta, D. B. Raudabaugh, K. Reschke, A. Rodriguez, M. Romero, S. Rooney-Latham, J. Roux, M. Sandoval-Denis, M. Th Smith, T. Steinrucken, T. Y. Svetasheva, E. J. van der Linde, M. V. D. Vegte, J. Vauras, A. Verbeken, C. M. Visagie, J. S. Vitelli, S. V. Volobuev, A. Weill, M. Wrzosek, I. Zmitrovich, E. A. Zvyagina, J. Z. Groenewald
Summary: This study describes novel species of fungi from various countries, including those living in different habitats such as grapevine vines, fruits, leaves, and roots. The results are supported by morphology and DNA barcodes.
Article
Mycology
Andrey Yurkov, Artur Alves, Feng-Yan Bai, Kyria Boundy-Mills, Pietro Buzzini, Neza Cadez, Gianluigi Cardinali, Serge Casaregola, Vishnu Chaturvedi, Valerie Collin, Jack W. Fell, Victoria Girard, Marizeth Groenewald, Ferry Hagen, Chris Todd Hittinger, Aleksey Kachalkin, Markus Kostrzewa, Vassili Kouvelis, Diego Libkind, Xinzhan Liu, Thomas Maier, Wieland Meyer, Gabor Peter, Marcin Piatek, Vincent Robert, Carlos A. Rosa, Jose Paulo Sampaio, Matthias Sipiczki, Marc Stadler, Takashi Sugita, Junta Sugiyama, Hiroshi Takagi, Masako Takashima, Benedetta Turchetti, Qi-Ming Wang, Teun Boekhout
Summary: The accurate application of fungal names is crucial for scientific communication, clinical diagnostics, and regulatory compliance. Maintaining the stability of the taxonomic system and ensuring the traceability of nomenclatural changes are key for a broad range of users and taxonomists. Proper preservation of nomenclatural history and physical organisms representing names is essential to assure the unambiguous application of names.
