期刊
FOOD CONTROL
卷 98, 期 -, 页码 297-302出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.foodcont.2018.11.040
关键词
Isothermal nucleic acid amplification; SEA; Meat authentication; Pork; On-site detection
资金
- National Natural Science Foundation of China [31670868, 21675094, 31800414]
- Shandong Province Natural Science Fund Major Basic Research Project [ZR2017ZC0123]
- Research Initial Funding Project for Doctors in Qingdao University of Science and Technology [010022908]
As meat adulteration issue is becoming increasingly prominent worldwide, it is crucial to realize on-site detection with simple equipment. Conventional nucleic acid amplification methods are reliable but the requirement of complex equipment, skilled technicians and long operation time limit their on-site use. Here, a simple denaturation bubble-mediated strand exchange amplification method (SEA) requiring only a pair of primers and one polymerase was first reported for identifying adulteration of pork source by targeting the specie-specific mitochondrial DNA sequence. The SEA method displayed good specificity for pork and could detect as low as 30 pg/mu L pork DNA. In binary mixtures, the SEA method could detect 1% pork meat total DNA by both colorimetric and fluorescence determination, fulfilling the requirement of artificial meat adulteration. Excitedly, the whole detection process could be finished within 1 h by coupling with fast tissue DNA extraction method, only requiring a simple heating block. Therefore, with simplicity and rapidity, SEA method will be suitable for on-site meat species identification.
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