Review
Biochemistry & Molecular Biology
Gehad G. Ibrahim, Jinyong Yan, Li Xu, Min Yang, Yunjun Yan
Summary: Resveratrol, a plant secondary metabolite, has various therapeutic applications and is in high demand. Recent efforts have focused on using yeast as a host for resveratrol production through metabolic engineering, showing promising results in increasing yields.
Review
Biotechnology & Applied Microbiology
Yanyan Wang, Linxia Liu, Zhaoxia Jin, Dawei Zhang
Summary: Vitamins are essential for maintaining normal metabolism and health, with wide applications in various industries. Using microbial cell factories for vitamin production is both environmentally friendly and economically sustainable, covering water-soluble and fat-soluble vitamins.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Article
Green & Sustainable Science & Technology
Zihe Liu, Hamideh Moradi, Shuobo Shi, Farshad Darvishi
Summary: Sustainable production of biofuels has become an attractive alternative to fossil fuels, with increasing interest in metabolic engineering of yeasts for biofuel production. Recent advancements in metabolic engineering have enabled the commercial production of new energy-dense biofuels using yeasts and synthetic biology tools.
RENEWABLE & SUSTAINABLE ENERGY REVIEWS
(2021)
Review
Biotechnology & Applied Microbiology
Shumin Xu, Song Gao, Yingfeng An
Summary: Pigments, such as carotenoids and melanins, are used in various aspects of daily life. The use of synthetic biology and metabolic engineering to construct microbial cell factories has advantages, such as a well-defined genetic background and rapid cell culture. However, the technical means for engineering microbial cell factories for pigment production and metabolic regulation processes have not been systematically analyzed. This review summarizes recent studies on the construction, modification, and regulation of synthetic pathways for microbial synthesis of pigments, providing new ideas for improving microbial production of natural pigments in the future.
BIOTECHNOLOGY ADVANCES
(2023)
Article
Green & Sustainable Science & Technology
Timothy D. H. Bugg, James J. Williamson, Fabrizio Alberti
Summary: This review discusses the use of engineered microbes for bioconversion of lignin from plant biomass to produce renewable chemicals, including existing bacterial hosts, case studies of engineered hosts, other potential hosts, and recent advances in genetic modification of fungi. It also explores prospects and challenges in the field, such as the choice of chassis organism, choice of lignin feedstock, complexity of polymeric lignin breakdown, and considerations for scale-up and choice of bioproduct.
RENEWABLE & SUSTAINABLE ENERGY REVIEWS
(2021)
Article
Cell & Tissue Engineering
Yoann Torres, Maude Gluais, Nicolas Da Silva, Sylvie Rey, Agathe Gremare, Laure Magnan, Fabien Kawecki, Nicolas L'Heureux
Summary: This study compares the ability of canine, porcine, ovine, and human skin fibroblasts to form cell-assembled extracellular matrix (CAM) sheets, highlighting significant species-dependent differences in CAM production and strength. Despite variations in serum and nutrient supplementation, ovine cells produced the most homogenous and strongest animal CAM sheets but were still about three times weaker than human sheets. This demonstrates critical species-to-species differences in fibroblast behavior and presents challenges in using animal cells for long-term tissue engineering constructs.
JOURNAL OF TISSUE ENGINEERING
(2021)
Review
Biotechnology & Applied Microbiology
Luan Luong Chu
Summary: Terpenoids, a group of secondary metabolites with diverse structures and functions, have various applications in human life. Engineering microbial cells using the CRISPR-Cas system offers a sustainable approach to produce terpenoids with high yields. This review summarizes the application of CRISPR-Cas system in terpenoid production and discusses its prospects.
CRITICAL REVIEWS IN BIOTECHNOLOGY
(2022)
Editorial Material
Multidisciplinary Sciences
Xiang Shi, Peining Chen, Huisheng Peng
Summary: Large-area display textiles can be produced by weaving transparent conductive weft and luminescent warp fibers using an industrial rapier loom. Integrating interactive functionalities, such as a keyboard and power supply, with the display textile forms an electronic textile system. Weaving textiles with integrated interactive functionalities allows them to serve as a communication tool.
Article
Biotechnology & Applied Microbiology
Jiao Meng, Shufan Liu, Le Gao, Kai Hong, Shuguang Liu, Xin Wu
Summary: This study elucidated a unique mechanism of P. pastoris for efficient methanol utilization, higher temperature resistance, and high protein synthesis, providing a P. pastoris cell factory for SCP production with environmental, economic, and nutritional benefits. Through adaptive laboratory evolution and metabolic engineering, the engineered strain produced high levels of SCP from methanol in a pilot-scale fed-batch culture, highlighting its potential in sustainable biomanufacturing.
MICROBIAL CELL FACTORIES
(2023)
Review
Agricultural Engineering
Jina Son, Kei-Anne Baritugo, Seo Hyun Lim, Hye Jin Lim, Seona Jeong, Ji Yeon Lee, Jong-il Choi, Jeong Chan Joo, Jeong-Geol Na, Si Jae Park
Summary: Currently, linear petroleum-based industries are causing depletion of finite natural resources and accumulation of non-biodegradable and hazardous wastes, highlighting the need for ecofriendly and sustainable solutions. Utilizing agro-industrial wastes as substrates can lead to the production of target value-added chemicals like organic acids, with advancements in metabolic engineering strategies and techniques enhancing their production. Further development and future perspectives in utilizing waste-based biorefineries for organic acid production from various industries have been discussed to showcase their importance.
BIORESOURCE TECHNOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Liang Guo, Mengya Qi, Cong Gao, Chao Ye, Guipeng Hu, Wei Song, Jing Wu, Liming Liu, Xiulai Chen
Summary: Engineering microbial cell viability (MCV) by regulating lifespan-specific genes to reduce reactive oxygen species (ROS) accumulation is a potential strategy to enhance chemical production in microbial cell factories.
METABOLIC ENGINEERING
(2022)
Article
Engineering, Environmental
Chong Li, Khai Lun Ong, Zhiyong Cui, Zhenyu Sang, Xiaotong Li, Raffel Dharma Patria, Qi Qingsheng, Patrick Fickers, Jianbin Yan, Carol Sze Ki Lin
Summary: This review summarizes recent advances in the use of solid organic wastes for efficient, green and sustainable succinic acid (SA) production. It covers the application, market and key global players of bio-based SA, achievements in SA production by promising yeasts, and costs comparison between oil-based substrates and raw materials.
JOURNAL OF HAZARDOUS MATERIALS
(2021)
Article
Biotechnology & Applied Microbiology
Mariam Dianat, Ute Muenchberg, Lars M. Blank, Erik Freier, Birgitta E. Ebert
Summary: In this study, we demonstrate the detection and monitoring of triterpenoids in living yeast cells using coherent anti-Stokes Raman scattering (CARS) and second-harmonic-generation (SHG) microscopy techniques. This method provides new insights into the dynamics of triterpenoid production and storage inside cells, which is crucial for rationalizing microbial engineering.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2023)
Editorial Material
Biochemistry & Molecular Biology
Natalja Kulagina, Nicolas Papon, Vincent Courdavault
Summary: Plants have long been a valuable source of natural products, but low accumulation of compounds in plants has been a challenge for production costs and supply. Recent advancements in metabolic engineering of microorganisms offer hope for the efficient production of these molecules at an industrial scale.
Article
Biochemistry & Molecular Biology
Abdelrhman Mohamed, Hannah M. Zmuda, Phuc T. Ha, Erik R. Coats, Haluk Beyenal
Summary: The combinatio of polarized electrodes with microbial fuel cells has led to the development of a switchable dual-mode bioelectrochemical wastewater treatment system, showing improved COD removal efficiency in both laboratory and pilot scale systems.
BIOELECTROCHEMISTRY
(2021)
Article
Toxicology
Kirt R. Phipps, Nigel J. Baldwin, Barry Lynch, Diane R. Stannard, Alica Soltesova, Ben Gilby, Marta H. Miks, Christoph H. Rohrig
JOURNAL OF APPLIED TOXICOLOGY
(2019)
Article
Toxicology
Kirt R. Phipps, Nigel J. Baldwin, Barry Lynch, Diane R. Stannard, Alica Soltesova, Ben Gilby, Marta H. Miks, Christoph H. Rohrig
JOURNAL OF APPLIED TOXICOLOGY
(2019)
Article
Gastroenterology & Hepatology
Cristina Iribarren, Hans Tornblom, Imran Aziz, Maria K. Magnusson, Johanna Sundin, Louise K. Vigsnaes, Ingvild Dybdrodt Amundsen, Bruce McConnell, Dorthe Seitzberg, Lena Ohman, Magnus Simren
NEUROGASTROENTEROLOGY AND MOTILITY
(2020)
Article
Toxicology
Kirt R. Phipps, Barry Lynch, Diane R. Stannard, Ben Gilby, Nigel Baldwin, Marta Hanna Miks, Annette Lau, Christoph H. Rohrig
Summary: Human milk oligosaccharides (HMOs) are bioactive molecules found in human breast milk, with potential applications in infant formula. The safety assessment of LNFP-I/2'-FL, a human-identical milk oligosaccharide, showed it to be non-genotoxic and safe for use in food products.
JOURNAL OF APPLIED TOXICOLOGY
(2021)
Article
Nutrition & Dietetics
Tanja Suligoj, Louise Kristine Vigsnaes, Pieter Van den Abbeele, Athanasia Apostolou, Katia Karalis, George M. Savva, Bruce McConnell, Nathalie Juge
Article
Gastroenterology & Hepatology
Cilius Esmann Fonvig, Ingvild Dybdrodt Amundsen, Louise Kristine Vigsnaes, Nikolaj Sorensen, Christine Frithioff-Bojsoe, Michael Christiansen, Paula Louise Hedley, Louise Aas Holm, Bruce McConnell, Jens-Christian Holm
Summary: This study found that both 2'FL and Mix are beneficial in increasing bifidobacteria in children's intestinal microbiota. Furthermore, supplementation with these substances is safe and well tolerated in children.
JOURNAL OF PEDIATRIC GASTROENTEROLOGY AND NUTRITION
(2021)
Review
Nutrition & Dietetics
Buket Soyyilmaz, Marta Hanna Miks, Christoph Hermann Rohrig, Martin Matwiejuk, Agnes Meszaros-Matwiejuk, Louise Kristine Vigsnaes
Summary: Human milk oligosaccharides (HMOs) are complex carbohydrates abundant in human milk, with over 200 different structures identified. The concentrations of HMOs in breast milk vary according to lactation period and mother's genetic status. Studies have shown significant variations in HMO concentrations for different types across lactation stages. In mature pooled human milk, the top 15 individual HMOs make up the majority of total HMO concentration.
Article
Microbiology
Louise Kristine Vigsnaes, Jonas Ghyselinck, Pieter van den Abbeele, Bruce McConnell, Frederic Moens, Massimo Marzorati, Danica Bajic
Summary: The study demonstrates the potential of human milk oligosaccharides (HMOs) in combating Clostridioides difficile infection (CDI) by reducing pathogen levels and enhancing beneficial microbial growth, aiding in the recovery of intestinal dysbiosis and prevention of CDI recurrence after antibiotic therapy.
Article
Nutrition & Dietetics
Cristina Iribarren, Maria K. Magnusson, Louise K. Vigsnaes, Imran Aziz, Ingvild Dybdrodt Amundsen, Tanja Suligoj, Nathalie Juge, Piyush Patel, Maria Sapnara, Lea Johnsen, Nikolaj Sorensen, Johanna Sundin, Hans Toernblom, Magnus Simren, Lena Oehman
Summary: This study showed that supplementation with 2’FL/LNnT can alter the gut microbiota, fecal and plasma metabolite profiles in IBS patients. The increase in bifidobacteria abundance was associated with these changes, while there was no effect on the host mucosal response.
Article
Toxicology
Kirt R. Phipps, Dayna Lozon, Diane R. Stannard, Ben Gilby, Nigel Baldwin, Marta Hanna Miks, Annette Lau, Christoph H. Rohrig
Summary: The study demonstrates the safety of biotechnologically produced 3-FL for use in infant formula, showing no adverse effects and supporting its potential benefits for infant growth and development.
JOURNAL OF APPLIED TOXICOLOGY
(2022)
Article
Microbiology
Greta Gecse, Andre Vente, Mogens Kilstrup, Peter Becker, Ted Johanson
Summary: This study investigates the impact of industrial CO2 levels on an Escherichia coli-based fermentation process. The results demonstrate that elevated CO2 levels can significantly affect the performance of microbial cultures, particularly in carbon/nitrogen-limited processes.
Article
Immunology
Maria Wiese, Frank H. J. Schuren, Wiep Klaas Smits, Ed J. J. Kuijper, Anita Ouwens, Margreet Heerikhuisen, Louise Vigsnaes, Tim J. J. van den Broek, Paulo de Boer, Roy C. C. Montijn, Jos M. B. M. van der Vossen
Summary: This study developed a 96-well CDi-screen platform using an adapted simulated ileal effluent medium, which is suitable for studying the proliferation of C. difficile in a complex gut ecosystem and screening for interventions that inhibit its growth.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Food Science & Technology
Alia H. Al-Khafaji, Stine Dam Jepsen, Kristine Rothaus Christensen, Louise Kristine Vigsnaes
JOURNAL OF FUNCTIONAL FOODS
(2020)
Review
Microbiology
Sylvester Holt, Marta H. Miks, Bruna Trindade de Carvalho, Maria R. Foulquie-Moreno, Johan M. Thevelein
FEMS MICROBIOLOGY REVIEWS
(2019)
Review
Biochemical Research Methods
Pei-Ru Chen, Peng-Fei Xia
Summary: Carbon dioxide plays a crucial role in mitigating global climate change and supporting life on Earth. Synthetic biology and metabolic engineering offer a promising approach to enhance CO2 fixation by combining the advantages of autotrophic and heterotrophic organisms. This article reviews the current progress in constructing synthetic CO2 fixation pathways, discusses the underlying design principles and challenges, and provides insights into the future of carbon recycling through engineered synthetic carbon fixation pathways.
CURRENT OPINION IN BIOTECHNOLOGY
(2024)