期刊
CURRENT MOLECULAR MEDICINE
卷 19, 期 7, 页码 506-524出版社
BENTHAM SCIENCE PUBL LTD
DOI: 10.2174/1566524019666190520094644
关键词
Candida albicans; homologous model; fluvastatin; docking study; in vitro study; ex vivo study
Background: The incidence of fungal infections has increased significantly. Specifically the cases of candida albicans infection are increasing day by day and their resistance to clinically approved drugs is a major concern for humans. Various classes of antifungal drugs are available in the market for the treatment of these infections but unfortunately, none of them is able to treat the infection. Objectives: Thus, in the present investigation, we have repurposed the well-known drug (Fluvastatin) in the treatment of Candida albicans infections by using in silico, in vitro and ex vivo techniques. Material and Methods: Computational and in vitro techniques. Results: Firstly, we developed and validated a simple model of CYP45014 alpha-lanosterol demethylase of Candida albicans by using crystal structure of Mycobacterium tuberculosis (1EA1). Further, fluvastatin was docked with a validated model of CYP45014 alpha-lanosterol demethylase and revealed good binding affinity as that of fluconazole. In vitro results (Percentage growth retardation, Fungal growth kinetics, Biofilm test and Post antifungal test) have shown good antifungal activity of fluvastatin. Finally, the results of MTT assay have shown non-cytotoxic effect of fluvastatin in murine splenocytes and thymocytes. Conclusion: However, further in vivo studies are required to confirm the complete role of fluvastatin as an antifungal agent.
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