Article
Biochemistry & Molecular Biology
Satoshi Katsube, Keiichiro Sakai, Tasuke Ando, Ryuta Tobe, Hiroshi Yoneyama
Summary: D-amino acids are found at high levels in the extracellular environment and are thought to have physiological functions, but how they are secreted remains unknown. This study discovered several potential d-alanine exporters in Escherichia coli and developed a screening system to evaluate their efficacy. The results suggest that AlaE and YciC are involved in the export of d-alanine, and overexpression of AlaE can alleviate growth constraints caused by high levels of d-alanine. This study also identified YciC as a novel d-alanine exporter.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Microbiology
Sara B. Hernandez, Felipe Cava
Summary: Peptidoglycan has been a key target for antibiotics in the battle against pathogenic bacteria, with recent advancements in techniques providing valuable resources for experts and newcomers in the field. This essential bacterial structure continues to be a focus of research, with improved understanding of its composition, architecture, and dynamics.
CURRENT OPINION IN MICROBIOLOGY
(2021)
Article
Chemistry, Multidisciplinary
Mohamed Labib, Laura Grabowski, Christian Bruesseler, Nicolai Kallscheuer, Luisa Wachtendonk, Thomas Fuchs, Andreas Jupke, Wolfgang Wiechert, Jan Marienhagen, Doerte Rother, Stephan Noack
Summary: This study achieved the microbial production of pyruvate and L-alanine in a one-pot fermentation process, and then transformed them into Metaraminol with high yields and stereoselectivity through a series of reactions.
ACS SUSTAINABLE CHEMISTRY & ENGINEERING
(2022)
Article
Plant Sciences
Giovanna Marta Fusco, Petronia Carillo, Rosalinda Nicastro, Giuseppe Carlo Modarelli, Carmen Arena, Stefania De Pascale, Roberta Paradiso
Summary: The impact of cold treatments on plant growth and flowering in Ranunculus asiaticus L. was investigated. The two hybrids, MBO and MDR, showed different responses to cold treatments, with MBO being less efficient in promoting flowering.
Article
Biochemistry & Molecular Biology
Yuka Muranaka, Miki Matsue, Asuka Mizutani, Masato Kobayashi, Kakeru Sato, Ami Kondo, Yuri Nishiyama, Shusei Ohata, Kodai Nishi, Kana Yamazaki, Ryuichi Nishii, Naoto Shikano, Shigefumi Okamoto, Keiichi Kawai
Summary: The World Health Organization has warned that antimicrobial resistance (AMR) is projected to cause 10 million deaths per year by 2050. In order to improve the diagnosis and treatment of infectious diseases, this study investigated the potential of amino acids as indicators of bacterial growth activity. It was found that H-3-L-Ala accurately reflects the proliferative activity of Escherichia coli K-12 and pathogenic EC-14, and detection methods using nuclear imaging may lead to expedited diagnostic treatments for infectious diseases.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Biotechnology & Applied Microbiology
Peng Yang, Wenjing Liu, Yanan Chen, An-Dong Gong
Summary: With growing concerns about environmental issues and sustainable economy, bioproduction of chemicals utilizing microbial cell factories provides an eco-friendly alternative to current petro-based processes. Creating high-performance strains (with high titer, yield, and productivity) through metabolic engineering strategies is critical for cost-competitive production. This review summarizes recent achievements in microbial production of chemicals through tuning of the glyoxylate cycle, with a focus on studies implemented in model microorganisms.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Review
Physiology
Henry J. Leese, Daniel R. Brison, Roger G. Sturmey
Summary: This article revisits the hypothesis proposed in 2002 that the successful development of oocytes and preimplantation mammalian embryos is associated with quiet metabolism. It introduces the concept of a Goldilocks zone, an optimal range of metabolic activity within which embryos with maximum developmental potential are located.
FRONTIERS IN PHYSIOLOGY
(2022)
Article
Chemistry, Organic
Souad Diana Tork, Emma Zsofia Aletta Nagy, Raluca Bianca Tomoiaga, Laszlo Csaba Bencze
Summary: An efficient preparative-scale synthetic procedure of L-phenylalanine derivatives has been developed using mutant variants of rigorous reaction engineering. The AtPAL-catalyzed hydroamination reaction of cinnamic acids provided several unnatural amino acids of high synthetic value, significantly surpassing the catalytic efficiency of previously reported PcPAL-based biotransformations. The AtPAL variants tolerated high substrate and product concentrations, representing an important extension of the PAL-toolbox, while the engineered biocatalytic procedures of improved E-factor and spacetime yields fulfill the requirements of sustainable and green chemistry, providing facile access to valuable amino acid building blocks.
JOURNAL OF ORGANIC CHEMISTRY
(2023)
Article
Biotechnology & Applied Microbiology
Huri Dedeakayogullari, Jarkko Valjakka, Ossi Turunen, Berin Yilmazer, Garip Demir, Janne Janis, Baris Binay
Summary: Unnatural amino acids, with amino and carboxylic acid functional groups and variable side chains, are important building blocks in modern medicinal chemistry. They can be synthesized from natural amino acids or through the use of enzymes, leading to novel molecules used in pharmaceutical production. This study focused on the reductive amination activity of the Thermomicrobium roseum alanine dehydrogenase enzyme, examining its potential with different substrates and the effects of metal ions on enzymatic activity. The enzyme showed promising reductive activity and may have applications in the synthesis of pharmaceutically relevant amino acids.
ENZYME AND MICROBIAL TECHNOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Anastasiia M. Zub, Roman D. Ostapiv, Bohdan O. Manko, Volodymyr V. Manko
Summary: This study investigated the effect of sodium pyruvate on pancreatic morphology and plasma amino acid levels in rats with acute pancreatitis. The results showed that sodium pyruvate improved plasma amino acid levels and partially restored the levels of certain amino acids when used in combination with sodium pyruvate. Therefore, sodium pyruvate may be considered as a maintenance therapy in acute pancreatitis.
Article
Ecology
Yang Yu, Peng Wang, Hai-Yan Cao, Zhao-Jie Teng, Yanping Zhu, Min Wang, Andrew McMinn, Yin Chen, Hua Xiang, Yu-Zhong Zhang, Xiu-Lan Chen, Yu-Qiang Zhang
Summary: A novel catabolic pathway for D-Glu was identified in marine bacteria and halophilic archaea, which helps to better understand the catabolism and recycling of D-Glu in these ecosystems. This study reveals the existence of two novel enzymes and a transcriptional regulator that play crucial roles in D-Glu catabolism. The findings highlight the importance of D-Glu as an overlooked pool of organic matter in global oceans.
Article
Public, Environmental & Occupational Health
Shaoxue Yang, Jie Song, Hong Yang, Wei Liu, Yuqing Jiang, Xiaohui Sun, Ding Ye, Yingying Mao, Songxiao Xu
Summary: The genetically predicted circulating concentrations of alanine and ALT were found to be associated with an altered risk of prostate cancer, indicating their potential roles in the development of the disease.
CLINICAL EPIDEMIOLOGY
(2022)
Article
Food Science & Technology
Meng Sun, Bintao Zhao, Zhixiang Cai, Juan Yan, Ruijuan Ma, Mingliang Yu
Summary: This study investigated and compared the amino acid profiles in peaches, revealing differences in amino acid concentrations and compositions among different varieties. Yellow and white flesh varieties had higher total amino acid concentrations, while some red flesh varieties had lower total amino acid concentrations. Several varieties with satisfactory amino acid concentrations and compositions were identified, which is important for producing high-quality peach wine with specific characteristics.
Article
Endocrinology & Metabolism
Daniel Ferguson, Sophie J. Eichler, Nicole K. H. Yiew, Jerry R. Colca, Kevin Cho, Gary J. Patti, Trevor M. Shew, Andrew J. Lutkewitte, Sandip Mukherjee, Kyle S. McCommis, Natalie M. Niemi, Brian N. Finck
Summary: The study evaluated the effects of MPC inhibitors (MPCi) on branched chain amino acid (BCAA) metabolism and found that MPCi can lower plasma BCAA concentrations and activate AMPK and mTOR signaling pathways. However, the effects on glucose homeostasis may be separate from BCAA concentrations.
MOLECULAR METABOLISM
(2023)
Article
Agriculture, Multidisciplinary
Xiumin Fu, Yinyin Liao, Sihua Cheng, Rufang Deng, Ziyin Yang
Summary: In tea plants, L-alanine is mainly located in vacuoles and mitochondria of root cells, while ethylamine is mainly found in the cortex with epidermis. Research discovered that in tea plants, L-alanine is converted to L-theanine via L-glutamate instead of ethylamine.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2021)
Article
Biotechnology & Applied Microbiology
Venkata Krishna Bayineni, Krishna Venkatesh, Chandan Kumar Sahu, Ravi-Kumar Kadeppagari
ENZYME AND MICROBIAL TECHNOLOGY
(2016)
Article
Food Science & Technology
Chandan Kumar Sahu, Ravi-Kumar Kadeppagari
INTERNATIONAL JOURNAL OF FOOD PROPERTIES
(2017)
Article
Chemistry, Analytical
Ushmaben Chandrakantbhai Dave, Deepak V. Ingale, Krishna Venkatesh, Venkata Krishna Bayineni, Ravi-Kumar Kadeppagari
JOURNAL OF ELECTROANALYTICAL CHEMISTRY
(2017)
Article
Biotechnology & Applied Microbiology
Venkata Krishna Bayineni, Sukrutha Suresh, Ashwani Sharma, Ravi-Kumar Kadeppagari
JOURNAL OF GENERAL AND APPLIED MICROBIOLOGY
(2018)
Article
Biochemistry & Molecular Biology
Ushmaben Chandrakantbhai Dave, Ravi-Kumar Kadeppagari
PROCESS BIOCHEMISTRY
(2018)
Article
Biotechnology & Applied Microbiology
Nallasamy Kumaresan, Konasur R. Sanjay, Kundumani S. Venkatesh, Ravi-Kumar Kadeppagari, Govindaswamy Vijayalakshmi, Sukumaran Umesh-Kumar
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2008)
Article
Biochemistry & Molecular Biology
Venkata Krishna Bayineni, Sukrutha Suresh, Gurmeet Singh, Ravi-Kumar Kadeppagari
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2014)
Article
Biochemistry & Molecular Biology
Natesan Sankar, Ravi-Kumar Kadeppagari, Bayar Thimmapaya
JOURNAL OF BIOLOGICAL CHEMISTRY
(2009)
Article
Virology
Ravi-Kumar Kadeppagari, Natesan Sankar, Bayar Thimmapaya
JOURNAL OF VIROLOGY
(2009)
Article
Biochemistry & Molecular Biology
N. Sankar, S. Baluchamy, R-K Kadeppagari, G. Singhal, S. Weitzman, B. Thimmapaya
Article
Food Science & Technology
Rajalakshmi Herale, Umesh-Kumar Sukumaran, Ravi-Kumar Kadeppagari
Review
Food Science & Technology
Sujatha Jayaraj, Sukrutha Suresh, Ravi-Kumar Kadeppagari
Article
Virology
Ravi-Kumar Kadeppagari, Rebecca L. Sanchez, Timothy P. Foster
Article
Biochemistry & Molecular Biology
Venkata Krishna Bayineni, Ravi-Kumar Kadeppagari
Summary: Plants are a rich source of lipase inhibitors, and bioautography is an effective method to detect these inhibitors from unexplored plants. Different parts of plants may exhibit varying levels of lipase inhibitory activities. The study demonstrates that bioautography can help to avoid false negatives and false positives, making it a reliable technique for screening lipase inhibitors in plants.
JOURNAL OF PLANT BIOCHEMISTRY AND BIOTECHNOLOGY
(2022)