期刊
CELLULAR REPROGRAMMING
卷 21, 期 3, 页码 141-151出版社
MARY ANN LIEBERT, INC
DOI: 10.1089/cell.2018.0054
关键词
cryptochrome 1; bone remolding; osteoblast differentiation; PI3K; AKT; MAPK; ERK
资金
- Key Department of Minhang District [2017MWTZ02]
- Key Department of the Fifth People's Hospital of Shanghai [2017WYZDZK02]
- Natural Science Foundation of Minhang District of Shanghai [2017MHZ15]
- Fifth People's Hospital of Shanghai, Fudan University [2018WYZT01]
- Minhang District Leading Talent Development Funds
The many circadian clock genes build up a network structure that controls physiological processes, such as the sleep cycle, metabolism, and hormone secretion. Cryptochrome 1 (CRY1), as one of the critical circadian proteins, is closely related to bone formation. However, the regulatory function of CRY1 in osteogenic differentiation remains unclear. In this study, we investigated the role of CRY1 in regulating proliferation and osteoblast differentiation in C3H10 and C2C12 cells after silencing Cry1 using short hairpin RNA interference. In vitro experiments confirmed that the expression level of CRY1 gradually increased during the osteogenic differentiation process, and Cry1 knockdown inhibited the proliferation and differentiation of osteoblastic cells. In addition, Cry1 knockdown inhibited the phosphorylation of AKT kinase (AKT) and extracellular signal-regulated kinase (ERK), which suppressed the phosphatidylinositol-4,5-bisphosphate 3-kinase (PI3K)-AKT and mitogen-activated protein kinase (MAPK)-ERK signaling pathways. Taken together, these findings show that CRY1 regulates the proliferation and differentiation of osteoblastic cells in an AKT and ERK-dependent manner.
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