期刊
CARBOHYDRATE POLYMERS
卷 209, 期 -, 页码 181-189出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.carbpol.2018.12.104
关键词
Hyaluronan; Doxorubicin; Polymeric micelles; Biodistribution; Pharmacokinetics
资金
- MEYS of the Czech Republic project INBIO [CZ.02.1.01/0.0/0.0/16_026/0008451]
A better understanding of in vivo behavior of nanocarriers is necessary for further improvement in their development. Here we present a novel approach, where both the matrix and the drug can be analyzed by LC-MS/MS after one sample handling. The developed method was applied for the comparison of pharmacokinetic profile of free and encapsulated doxorubicin (DOX) in oleyl hyaluronan (HA-C18:1) polymeric micelles. The results indicated that nanocarriers were rapidly dissociated upon in vivo administration. Despite this fact, the administration of encapsulated DOX led to its longer circulation time and enhanced tumor targeting. This effect was not observed injecting blank HA-C18:1 micelles followed by unencapsulated DOX. Biodistribution studies and molecular weight estimation of the carrier matrix indicated relatively high stability of HA-C18:1 ester bond in bloodstream and complete elimination of the derivative within 72 h. The proposed methodology provides a novel strategy to elucidate the pharmacokinetic behavior of polysaccharide-based drug delivery systems.
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