期刊
BMC MICROBIOLOGY
卷 19, 期 -, 页码 -出版社
BMC
DOI: 10.1186/s12866-019-1454-2
关键词
bla(NDM-5); Carbapenemase; Escherichia coli; Multidrug resistance; Poultry farm
类别
资金
- Public Welfare Technology Applied Research Projects in Zhejiang Province Project [2017C32021]
- Natural Science Foundation for the Youth [31700007]
- Youth Talent Training and International Cooperation program of Zhejiang Academy of Agricultural Sciences [2017R19R08E01]
- Key research and development plan of Zhejiang Province [2015C02041]
- State Key Laboratory Breeding Base for Zhejiang Sustainable Pest and Disease Control [2011DS700124-ZZ1703]
BackgroundThe emergence of carbapenem-resistant Enterobacteriaceae strains has posed a severe threat to public health in recent years. The mobile elements carrying the New Delhi metallo--lactqtamase (NDM) gene have been regarded as the major mechanism leading to the rapid increase of carbapenem-resistant Enterobacteriaceae strains isolated from clinics and animals.ResultsWe describe an NDM-5-producing Escherichia coli strain, ECCRA-119 (sequence type 156 [ST156]), isolated from a poultry farm in Zhejiang, China. ECCRA-119 is a multidrug-resistant (MDR) isolate that exhibited resistance to 27 antimicrobial compounds, including imipenem and meropenem, as detected by antimicrobial susceptibility testing (AST). The complete genome sequence of the ECCRA-119 isolate was also obtained using the PacBio RS II platform. Eleven acquired resistance genes were identified in the chromosome; four were detected in plasmid pTB201, while six were detected in plasmid pTB202. Importantly, the carbapenem-resistant gene bla(NDM-5) was detected in the IncX3 plasmid pTB203. In addition, seven virulence genes and one metal-resistance gene were also detected. The results of conjugation experiments and the transfer regions identification indicated that the bla(NDM-5)-harboring plasmid pTB203 could be transferred between E. coli strains.ConclusionsThe results reflected the severe bacterial resistance in a poultry farm in Zhejiang province and increased our understanding of the presence and transmission of the bla(NDM-5) gene.
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