期刊
BMC CANCER
卷 19, 期 -, 页码 -出版社
BMC
DOI: 10.1186/s12885-019-5565-9
关键词
Biomarker discovery; Chemoradiation therapy; Extracellular vesicles; Head and neck squamous cell carcinoma; HNSCC; Treatment response
类别
资金
- National Cancer Centre of Singapore Research Foundation [NMRC/CSA-INV/011/2016]
- Fundacao de Amparo a Pesquisa do Estado de Sao Paulo -FAPESP (FAPESP) [2015/21420-3]
- Exploit Technologies Pte and Biomedical Research Council (A*STAR) [ETPL/12-R15GAP-0010]
BackgroundThere is a paucity of plasma-based biomarkers that prospectively segregate the outcome of patients with head and neck squamous-cell carcinoma (HNSCC) treated with chemoradiation therapy (CRT). Plasma extracellular vesicles (EVs) might be an alternative source for discovery of new specific markers present in patients with HNSCC, which could help to re-direct patients to appropriate curative therapies without delay.MethodsIn order to identify new markers in plasma compartments, Cholerae toxin B chain (CTB) and Annexin V (AV) were used to isolate EVs from pooled plasma samples from patients with locally advanced HNSCC who responded (CR, n=6) or presented incomplete response (NR, n=6) to CRT. The crude plasma and EVs cargo were screened by antibody array.ResultsOf the 370 polypeptides detected, 119 proteins were specific to NR patients while 38 were exclusive of the CR subjects. The Gene Set Enrichment Analysis (GSEA) and Search Tool for the Retrieval of Interacting Genes (STRING) database analysis indicated that the content of circulating plasma EVs might have a relevant function for the tumor intercellular communication in the HNSCC patients.ConclusionThis study provides a list of potential markers present in plasma compartments that might contribute to the development of tools for prediction and assessment of CRT response and potentially guide therapeutic decisions in this context.
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