期刊
BIOTECHNIQUES
卷 66, 期 6, 页码 285-289出版社
FUTURE SCI LTD
DOI: 10.2144/btn-2019-0016
关键词
bacterial load; DNA extraction; high throughput; low bacterial load; metagenomics; microbiome
资金
- ALSAC
A major challenge for microbiome studies is maintaining an even and accurate DNA extraction in the presence of samples with a wide range of bacterial content. Here we compare five DNA extraction methods using replicate stool samples that were diluted to create high and low biomass samples. Our results indicate greater variation in microbiome composition between high and low biomass samples than variation between methods. Many of the extraction methods had reduced yield from low biomass samples; however, our adapted plate column-based extraction method was evenly efficient and captured the largest number of high-quality reads. Based on these results, we have identified a DNA extraction method that ensures adequate yield in metagenomic microbiome studies that have samples with a broad range of bacterial content. METHOD SUMMARY A high-throughput DNA extraction method for studies that have samples with a broad range of bacterial content for 16S rRNA gene amplicon and shotgun metagenomic sequencing analyses. This consists of enzyme pretreatment, followed by adapting a plate column-based protocol to increase yield from low biomass samples.
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