Article
Chemistry, Analytical
Xuan Wang, Chenfei Xu, Yuxian Wang, Wei Li, Zhengbo Chen
Summary: The research team developed an electrochemical sensor based on T-Hg2+-T coordination, which can accurately and sensitively detect Hg2+. The sensor has a wide linear range and low detection limit, and successfully detected Hg2+ in river water samples, demonstrating its potential for environmental monitoring.
SENSORS AND ACTUATORS B-CHEMICAL
(2021)
Article
Chemistry, Analytical
Narjiss Seddaoui, Aziz Amine
Summary: A colorimetric smartphone-based immunoassay was developed for on-site detection of pork adulteration in meat, providing rapid, sensitive, and portable analytical method. The method demonstrated excellent precision and accuracy in detecting as low as 0.01% of pork in meat mixtures, with high stability and selectivity towards pork.
Article
Chemistry, Analytical
Miao Zhong, Shengyuan Yang, Lei Chen, Chen Liu, Jiao Shi, Hao Liang, Xilin Xiao, Le Li, Jinquan Liu
Summary: A novel fluorescent sensing method for highly sensitive detection of Hg2+ was developed using a triple-helix molecular switch (THMS) and exonuclease III (Exo III)-assisted signal amplification. The method showed a linear detection range of 0.01-50 nM and could detect Hg2+ at concentrations as low as 1.04 pM. It demonstrated potential applications in environmental and food monitoring.
ANALYTICA CHIMICA ACTA
(2022)
Article
Chemistry, Analytical
Yue Wang, Hongguo Zhai, Jiaqi Yin, Qi Guo, Yuhao Zhang, Qingqing Yang, Falan Li, Xia Sun, Yemin Guo, Yanyan Zhang
Summary: In this study, a dual-target electrochemical biosensor was designed based on silver nanoparticles and exonuclease I-assisted cycle signal amplification strategy to detect Pb2+ and Hg2+ in water. The biosensor exhibited high sensitivity, low detection limits, good reproducibility, and recovery rate. The cycle amplification strategy supported by this enzyme has promising applications in metal ion detection.
Article
Chemistry, Analytical
Xiaotao Zhang, Yi Wei, Huihuang Wu, Hongli Yan, Yiming Liu, Zeljka Lucev Vasic, Haibo Pan, Mario Cifrek, Min Du, Yueming Gao
Summary: On-site quantitative detection devices play a crucial role in healthcare, clinical diagnosis, environmental monitoring, and food analysis. A smartphone-based electrochemical detection device shows excellent performance with wider voltage and current range, as well as higher detection resolution.
Article
Chemistry, Analytical
Xiaofang Zhao, Yipian Liu, Bao Shu, Zongning Guo, Chao Song, Xuelin Huang, Zhe Jiao
Summary: In this paper, a smartphone-based portable device was designed for on-site and real-time detection of Hg2+ concentration levels in water samples. The device uses a specific fluorescent probe and analyzes the data through a smartphone application. The method is simple, cost-effective, portable, and enables real-time data sharing.
INTERNATIONAL JOURNAL OF ENVIRONMENTAL ANALYTICAL CHEMISTRY
(2022)
Article
Electrochemistry
Qingxia Ran, Hui Feng, Guorui Chang, Meng Luo, Shuxia Xu
Summary: In this study, a thymine-mediated electrochemical aptasensor was developed for the simultaneous detection of Hg2+ and CH3Hg+ in fish muscle. The sensor utilized ferrocene and methylene blue as electrochemical tags. The sensing strategy allowed for sensitive and selective detection of Hg2+ and CH3Hg+ without the need for extra separation procedures, achieving detection limits of 0.06 pM and 0.13 pM, respectively.
ELECTROCHIMICA ACTA
(2023)
Article
Biophysics
Yuan Cheng, Hongliang Wang, Yuxin Zhuo, Dan Song, Chunsheng Li, Anna Zhu, Feng Long
Summary: This paper presents an innovative reusable smartphone-facilitated mobile fluorescence biosensor that combines optical system, microfluidic system, and smartphone to achieve rapid detection and quantitative analysis of toxic pollutants.
BIOSENSORS & BIOELECTRONICS
(2022)
Article
Multidisciplinary Sciences
Kateryna Trofymchuk, Viktorija Glembockyte, Lennart Grabenhorst, Florian Steiner, Carolin Vietz, Cindy Close, Martina Pfeiffer, Lars Richter, Max L. Schuette, Florian Selbach, Renukka Yaadav, Jonas Zaehringer, Qingshan Wei, Aydogan Ozcan, Birka Lalkens, Guillermo P. Acuna, Philip Tinnefeld
Summary: The development of highly sensitive photodetectors and photostabilization strategies has made single-molecule fluorescence detection a routine task in labs worldwide. Simplifying single-molecule detection could enable exciting applications in various settings, such as diagnostic points. A new type of addressable NanoAntennas with Cleared HOtSpots (NACHOS) was introduced, allowing for detection using a standard smartphone camera and inexpensive objective lens.
NATURE COMMUNICATIONS
(2021)
Article
Chemistry, Analytical
Kai Shi, Jiaxuan Chen, Yuhao Li, Qiong Li, Jiuhua Song, Zhigang Yi, Daxiu Li, Jiaheng Zhang
Summary: In this study, a portable on-site biosensor was constructed using CRISPR-Cas12a for quantitative, sensitive, and selective detection of Hg2+. The biosensor utilized a target-triggered cascade toehold-mediated strand displacement reaction to activate the cleavage activity of Cas12a, resulting in the detection of Hg2+. The biosensor exhibited a wide dynamic range and low detection limit.
ANALYTICA CHIMICA ACTA
(2023)
Article
Chemistry, Analytical
Tingting Liu, Yuhao Li, Jingjing Gu, Lei Zhang, Feiyue Qian, Binrong Li, Xuedong Wang
Summary: Modulating the catalytic activity of nanozymes is essential for their practical applications in target analyte detection. In this study, a nanocomposite (NSC/Co6Ni3S8) consisting of N, S co-doped carbon and Co6Ni3S8 was fabricated using a simple solgel approach. Compared to NSC/Ni9S8, NSC/Co6Ni3S8 with bimetallic active sites exhibited superior enzyme-mimetic activity. The nanocomposite could catalyze O-2 to generate center dot O-2(-) and oxidize colorless 3, 3', 5, 5'-tetramethylbenzidine (TMB) into blue oxTMB. Hg2+ could interact with S-2 to facilitate O-2 adsorption, leading to enhanced color development. A colorimetric sensing platform for Hg2+ detection was established based on the oxidase-like activity of NSC/Co6Ni3S8 and Hg2+-triggered promoting effect, exhibiting a linear range of 10-200 μg/L and a detection limit of 3 μg/L. By integration with a smartphone-based APP Thing Identify software, a visual colorimetric assay for Hg2+ was developed with a lower detection limit of 5 μg/L compared to the mercury vapor meter. The NSC/Co6Ni3S8-based colorimetric assay is convenient, rapid, and visual, making it suitable for routine monitoring of Hg2+ in real-world waters under outdoor conditions.
ANALYTICA CHIMICA ACTA
(2023)
Review
Multidisciplinary Sciences
Bangfeng Wang, Yiwei Li, Mengfan Zhou, Yulong Han, Mingyu Zhang, Zhaolong Gao, Zetai Liu, Peng Chen, Wei Du, Xingcai Zhang, Xiaojun Feng, Bi-Feng Liu
Summary: The frequent outbreak of global infectious diseases has led to the development of rapid and effective diagnostic tools for early screening of potential patients. Researchers have shown significant interest in smartphone-based mobile health platforms that integrate microfluidic optical detection with artificial intelligence analysis. This article summarizes recent progress in these platforms, including microfluidic chips, imaging modalities, supporting components, and software algorithms. The application of these platforms in detecting various objects, such as molecules, viruses, cells, and parasites, is also discussed. Future directions for the development of mobile health platforms are addressed.
NATURE COMMUNICATIONS
(2023)
Article
Biochemistry & Molecular Biology
Keyan Chen, Shiqi Fu, Chenyu Jin, Fan Guo, Yu He, Qi Ren, Xuesheng Wang
Summary: A smartphone-assisted method for the on-site detection of Hg2+ and Cl- using fluorescence and colorimetry was developed based on the oxidase-like activity of Au-Hg alloy on Au/Cu/Ti3C2 nanosheets. The method involved the in-situ growth of Au nanoparticles on Cu/Ti3C2 nanosheets and subsequent characterization. Addition of Hg2+ promoted the oxidation of o-phenylenediamine (OPD) to generate a fluorescence emission peak, enabling the detection of Hg2+. The fluorescence intensity of 2,3-diaminopenazine (ADP) was quenched by the addition of Cl-, allowing for the detection of Cl-. The method exhibited good linear ranges and detection limits for Hg2+ and Cl-. Additionally, a colorimetric method utilizing digital images and smartphones was developed. This Au/Cu/Ti3C2 nanosheets-based method provides a novel and comprehensive application for environmental monitoring.
Article
Engineering, Environmental
Yidan Ma, Yujie Yu, Xinyi Mu, Chao Yu, Yuan Zhou, Jun Chen, Shuting Zheng, Junlin He
Summary: In this study, a novel dual-analytical platform was developed for visual, sensitive, and reliable analysis of mercury ions in environmental water samples. The method utilized thymine-rich DNA probes and luminescent metalpolydopamine frameworks to achieve accurate detection of Hg2+, with potential applicability for monitoring environmental water samples. Additionally, a multicolorimetric system based on a smartphone was established for on-site determination.
JOURNAL OF HAZARDOUS MATERIALS
(2021)
Article
Chemistry, Applied
Jie Fang, Xiang Li, Dandan Pi, Chao Gao, Sihao Gao, Wei Li, Wu Yan, Hantao Zou
Summary: A novel conjugated polymer-based ratiometric fluorescent probe PFTQ has been synthesized for the quantitative detection of bisulfite in food samples. The probe can change emission color upon reaction with bisulfite and convert the signal into digital values for accurate measurement. This sensing platform enables fast and accurate detection of bisulfite in various food samples.
Article
Biochemistry & Molecular Biology
Guolei Tan, Zhongjie Fei, Rongbin Wei, Xuping Wu, Pengfeng Xiao
Summary: The study developed a new bioluminescent pyrophosphate detection method to increase the sensitivity of hepatitis B virus detection, surpassing traditional methods and providing rapid analysis. By using 2-deoxyadenosine-5-(alpha-thio)-triphosphate instead of dATP, the detection method was improved and the results were stable and reliable.
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
(2022)
Article
Optics
Xinyi Xing, Lin Zhu, Chao Chen, Nan Sun, Chuankai Yang, Keding Yan, Liang Xue, Shouyu Wang
Summary: This study proposes a new method for evaluating the quality of transformer oil based on quantitative phase microscopy. By using a phase real-time microscopic camera, under- and over-focus images of gas bubbles in transformer oil can be simultaneously captured, and the oil-to-gas-volume ratio can be computed to evaluate the quality of transformer oil. Compared with traditional methods, this approach has the advantages of simple operation and low cost.
Article
Computer Science, Interdisciplinary Applications
Keding Yan, Shuwei Yang, Yingran Zhao, Chao Ma, Ying Jin, Shouyu Wang
Summary: The paper introduces a technique to obtain stable light scattering fields directly through deep learning methods without the need for statistical processes, and demonstrates a case of reconstructing light scattering fields from 1-D randomly rough surfaces. Compared to traditional high-accuracy but low-efficiency methods, this deep learning method provides accurate scattering results and significantly speeds up the processing.
COMPUTER PHYSICS COMMUNICATIONS
(2022)
Article
Optics
Qiang Lu, Fake Lu, Xiaoliang He, Zhilong Jiang, Hongyan Lu, Fan Zhu, Cheng Liu, Shouyu Wang, Yan Kong
Summary: A numerical model for multipulse laser drilling is constructed, and it is found that temperature accumulation, thermal stress, and crater morphology change induced by previous laser pulses promote subsequent pulse laser drilling.
Article
Biophysics
Qin Zhang, Jiahao Li, Yue Li, Guolei Tan, Mei Sun, Yanke Shan, Yue Zhang, Xin Wang, Keyu Song, Rui Shi, Ling Huang, Fei Liu, Yongxiang Yi, Xuping Wu
Summary: The development of reliable and fast diagnostic devices for COVID-19 is crucial. In this study, a new analysis principle combining reverse transcription and isothermal amplification with CRISPR/Cas13a reaction was proposed. The method showed good sensitivity and specificity in detecting the SARS-CoV-2 gene fragment, and results could be obtained within 1 hour using a portable device. This approach has the potential for point-of-care screening in remote areas with limited resources.
BIOSENSORS & BIOELECTRONICS
(2022)
Article
Optics
Yuanjie Gu, Zhibo Xiao, Wei Hou, Cheng Liu, Ying Jin, Shouyu Wang
Summary: This study proposes the first deep low-excitation fluorescence imaging enhancement solution to reconstruct optimized-excitation fluorescence images, aiming at reducing photobleaching and phototoxicity. By using the new framework Kindred-Nets and a mixed fine-tuning tactic, high-contrast optimized-excitation fluorescence images can be obtained effectively, avoiding the dimming effect caused by ineffective features.
Review
Virology
Wei Hou, Sibei Wang, Heqiong Wu, Linli Xue, Bin Wang, Shouyu Wang, Haidong Wang
Summary: This article discusses the role of small GTPases in the coronavirus life cycle and suggests the potential of targeting small GTPases as targets for adjuvant development.
Article
Optics
Shuaichao Wang, Xiali Gao, Pengfei Zhu, Xiaoliang He, Zhilong Jiang, Yan Kong, Shouyu Wang, Cheng Liu
Summary: A three-wavelength photoelasticity method is proposed to simplify the optical setup and speed up data acquisition. By recording six intensity images with circularly polarized illuminations of three close wavelengths, accurate computation of phase retardation and inner stress can be achieved. The method avoids the mechanical rotations required by classic photoelasticity techniques, enabling speedy data acquisition and measurement of dynamic samples using a simple and compact optical setup. The feasibility of this suggested three-wavelength digital photoelasticity method is confirmed through theoretical analyses, numerical simulations, and experimental evidence.
Article
Optics
Lin Zhu, Zhibo Xiao, Chao Chen, Aihui Sun, Xiaoliang He, Zhilong Jiang, Yan Kong, Liang Xue, Cheng Liu, Shouyu Wang
Summary: sPhaseStation is a label-free whole slide quantitative phase imaging system based on transport of intensity phase microscopy, which can capture high-resolution digital images of microscope slides and provide accurate phase retrieval.
Article
Optics
Zhibo Xiao, Yuanjie Gu, Lin Zhu, Cheng Liu, Shouyu Wang
Summary: To obtain an image with high spatial resolution and a large field of view, the Deep SBP+ framework is designed. It combines a low-resolution image with a large field of view and several high-resolution images in smaller fields of view to reconstruct an image with both high resolution and a large field of view. The Deep SBP+ breaks through the trade-off between spatial resolution and field of view, providing a simpler and faster method for reconstructing high-resolution and large field of view images compared to conventional methods relying on spatial and spectral scanning.
JOURNAL OF THE OPTICAL SOCIETY OF AMERICA A-OPTICS IMAGE SCIENCE AND VISION
(2023)
Article
Optics
Zhibo Xiao, Yinghan Guan, Ying Jin, Wei Hou, Yuanjie Gu, Shouyu Wang
Summary: Lowering excitation to reduce phototoxicity and photobleaching while enhancing fluorescence signal is useful for long-term observation in fluorescence microscopy. However, invalid features such as dark backgrounds negatively impact neural networks, making it challenging to extend deep learning-based image enhancement methods to fluorescence imaging. To address this, we propose Deep-Gamma, a deep low-excitation fluorescence imaging global enhancement framework with a self-attention module called GammaAtt that avoids negative optimization. Deep-Gamma produces higher-quality fluorescence-enhanced images compared to other state-of-the-art methods and has significant potential in medical imaging applications.
Article
Infectious Diseases
Ruixian Zhang, Sha Luo, Nan Wang, Hongying Zhang, Xuping Wu
Summary: The study evaluated the minimal inhibitory concentration (MIC) of bedaquiline, clofazimine, linezolid, delamanid, and pretomanid for the treatment of Mycobacterium abscessus and Mycobacterium intracellulare in vitro. The results showed that bedaquiline, clofazimine, and linezolid were more effective in inhibiting the growth of these bacteria, while delamanid and pretomanid had limited efficacy. The mutation in the MAB_0540 gene may be associated with clofazimine resistance in M. abscessus.
INFECTION AND DRUG RESISTANCE
(2023)
Article
Optics
Aihui Sun, Feng Gao, Pengfei Zhu, Xiaoliang He, Zhilong Jiang, Yan Kong, Shouyu Wang, Cheng Liu
Summary: To improve the performance of binary diffuser-based coherent modulation imaging (CMI), a double-channel optical alignment was proposed, which captured two diffraction patterns formed by the reflection and transmission of a binary diffuser and adopted them for iterative reconstruction. Besides, the reflected light information, neglected in traditional single-channel optical alignment, was also reconstructed in this dual-channel DB-CMI. Both numerical simulations and proof-of-principle experiments confirmed the improved reconstruction quality and speed. Therefore, DB-CMI enhances traditional CMI and serves as a powerful tool for quantitative phase imaging.
Article
Immunology
Nan Wang, Xia Xiong, Zhiqi Liu, Ruixian Zhang, Sha Luo, Hongying Zhang, Xuping Wu
Summary: This study investigated the prevalence of integrase inhibitor resistance-related mutations and other drug resistance-related mutations among newly diagnosed ART-naive HIV-positive patients in Nanjing, China. It was found that a few patients had major integrase resistance-related mutations and others had accessory resistance-related mutations. These results highlight the importance of further molecular surveillance-based monitoring of the HIV epidemic in Nanjing.
MICROBIAL PATHOGENESIS
(2023)
Article
Chemistry, Analytical
Lin Zhu, Shuwei Yang, Zhibo Xiao, Huachuan Huang, Keding Yan, Shouyu Wang
Summary: We developed a portable spectrometer based on Raspberry Pi, incorporating a white LED as the light source, a reflection grating for dispersion, and a CMOS imaging chip for spectral recording. All optical elements and Raspberry Pi were integrated using 3-D printed structures, and a custom software was designed for recording, calibration, analysis, and display. With a built-in battery, the portable spectrometer enables on-site applications. Tested and verified, it provides a spectral resolution of 0.065 nm per pixel in the visible band, making it suitable for on-site spectral testing in various fields.
ANALYTICAL METHODS
(2023)
Article
Biophysics
Reza Khodadadi, Manouchehr Eghbal, Hamideh Ofoghi, Alireza Balaei, Ali Tamayol, Karen Abrinia, Amir Sanati-Nezhad, Mohamadmahdi Samandari
Summary: This paper introduces an integrated portable centrifugal microfluidic system that automates cell and fluid manipulation for complete blood counting (CBC) analysis at the point-of-care (POC). The system utilizes a specially designed microfluidic disc for cell separation, solution metering and mixing, and cell counting, and is equipped with a custom script for automated quantification of cells. The proposed method shows a strong correlation with the gold standard hematology analyzer for various blood parameters. The portable system offers simplicity, affordability, and low power consumption, making it a potential solution for improving healthcare delivery in resource-limited settings and remote areas.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Nabil H. Bhuiyan, Joon S. Shim
Summary: Developing herd immunity is crucial for changing the course of the COVID-19 pandemic. An AI-driven point-of-care testing platform has been proposed for analyzing the body's response to SARS-CoV-2, and it has been successfully used for quantifying viral proteins and antibodies in blood samples. A data-receptive web-based dashboard system has also been developed for real-time analysis of herd immunity progress.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Alexiane Godain, Timothy M. Vogel, Pascal Fongarland, Naoufel Haddour
Summary: This study provides comprehensive insights into the intricate relationship between shear stress and electroactive biofilms in microbial fuel cells, highlighting the pivotal role of shear stress in influencing the growth kinetics, electrical performance, and physical structure of the biofilms. The study also emphasizes the complexities of extracellular electron transfer mechanisms and the need for complementary metaproteomic analyses.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Linjie Wang, Yixin Chen, Yang Ji, Shujun Zheng, Fei Wang, Caolong Li
Summary: A paper-based biosensor incorporating surface-enhanced Raman spectroscopy (SERS) and colorimetric detection has been developed for efficient quantification of intracellular glutathione (GSH). The biosensor exhibits excellent selectivity, stability, and precision, with low detection limits in both SERS and colorimetric modes. It has been successfully utilized for intracellular GSH detection and validated against a commercial GSH assay kit.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Quan Zhou, Jing Wang, Zhen Zhang, Alain Wuethrich, Richard J. Lobb, Matt Trau
Summary: This study presents a biosensor for sensitive detection of EMT-associated biomarkers on extracellular vesicles (EVs) surfaces during targeted therapy. Through longitudinal monitoring of patients, the biosensor shows its ability to identify the EMT-like phenotype switching on circulating EVs during the development of resistance.
BIOSENSORS & BIOELECTRONICS
(2024)
Review
Biophysics
Pratika Rai, Sabrina N. Hoba, Celine Buchmann, Robert J. Subirana-Slotos, Christian Kersten, Tanja Schirmeister, Kristina Endres, Bernd Bufe, Alexey Tarasov
Summary: Proteases have been proposed as potential biomarkers for various pathological conditions. The development of protease biosensors offers a more efficient way to investigate dysregulated proteases. This review article presents different optical and electrochemical detection methods for designing biosensors for all major protease families.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Chengxin Liu, Xu Shen, Li Yan, Runlian Qu, Yue Wang, Yaqin He, Zixuan Zhan, Piaopiao Chen, Feng Lin
Summary: In this study, a homogeneous rapid electrochemical aptasensor was developed to quantitatively detect CTCs in lung cancer patients. The aptasensor utilized a DNA nanosphere structure and a complementary aptamer to specifically detect mucin 1 as a marker for CTCs. The assay exhibited high specificity and sensitivity, and the results were consistent with other detection methods.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Danni Xue, Xing Dai, Jialong Zhao, Jiayao Zhang, Huan Liu, Kui Liu, Tao Xu, Chenjie Gu, Xingfei Zhou, Tao Jiang
Summary: In this study, a dual-enhancement SERS substrate based on Ti3C2TX and Ag nanocubes was fabricated for precise quantification of ritonavir and ibrutinib in serum. The formation of numerous electromagnetic hotspots between Ag nanocubes facilitated effective photo-induced charge transfer. The composite substrate showed excellent sensitivity, achieving low detection limits and high recoveries, making it promising for monitoring and identification of clinical blood drug concentration.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Tenghua Zhang, Zihui Xie, Xiaohe Zheng, Yuxin Liang, Yao Lu, Hankang Zhong, Feiyang Qian, Yuqing Zhu, Ruiting Sun, Yan Sheng, Jiaming Hu
Summary: This study reports a technology based on cationic lipid-polymer hybrid nanoparticles for efficient extracellular vesicle (EV) enrichment and in-situ detection of internal microRNAs. The technology demonstrates high EV enrichment efficiency and sensitive internal RNA detection, making it potentially useful for early pancreatic cancer diagnosis.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Wenjie Dai, Gaoxu Chen, Xiaoyan Wang, Shujun Zhen, Chengzhi Huang, Lei Zhan, Yuanfang Li
Summary: In this study, a novel metal organic gel (MOG) with dual electrochemiluminescence (ECL) properties was prepared by simple mixing. The MOG exhibited strong and stable anodic and cathodic ECL signals. By utilizing this MOG, an ECL resonance energy transfer (ECL-RET) biosensor was constructed for ultrasensitive detection of a specific gene. The study developed a straightforward technique for obtaining a single luminescent material with dual signals and expanded the analytical application of MOGs in the realm of ECL.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Lais Canniatti Brazaca, Amanda Hikari Imamura, Rodrigo Vieira Blasques, Jessica Rocha Camargo, Bruno Campos Janegitz, Emanuel Carrilho
Summary: The use of microfluidic paper-based analytical devices (muPADs) for medical diagnosis is a growing trend due to their low cost, easy use, simple manufacturing, and potential for application in low-resource settings. This review focuses on the advances in muPADs for medical diagnostics, discussing their use in detecting various biomarkers in common human biofluids. The challenges of biomarker detection in each sample are examined, along with innovative techniques to overcome these limitations. The commercialization difficulties of muPADs are also considered, along with future trends such as wearable devices and integrated platforms.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Minghong Jian, Xudong Sun, Hua Zhang, Xiaotong Li, Shasha Li, Zhenxin Wang
Summary: Matrix metalloproteinases (MMPs) are attractive biomarkers for cancer diagnosis and treatment, but their low abundance in biological samples, especially in the early stages of tumors, makes it challenging to precisely analyze MMP activities. In this study, a peptide microarray-based metal-enhanced fluorescence assay (PMMEFA) is proposed as a sensitive and specific method to simultaneously detect MMP-1, -2, -3, -7, -9, and -13 activities. The PMMEFA showed excellent sensitivity and was capable of detecting MMP activities in various matrices.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Kyung Ho Kim, Eunsu Ryu, Zinah Hilal Khaleel, Sung Eun Seo, Lina Kim, Yong Ho Kim, Hyun Gyu Park, Oh Seok Kwon
Summary: We have developed a novel strategy for discriminative detection of SARS-CoV-2 variants using the plasmonic photothermal effect of gold nanofilms and digital polymerase chain reaction (dPCR) technology. With this method, we were able to detect the delta and omicron variants with high sensitivity within 25 minutes from COVID-19 patients' clinical samples, making it a rapid and accurate point-of-care testing tool.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Zepeng Kang, Yuanming Wang, Haiyan Song, Xueli Wang, Job Zhang, Zhiguang Zhu
Summary: By designing a wearable and flexible lactic-acid/O2 EBFC with an air-breathing biocathode, the limitations of biocathode are effectively solved. The optimal performance conditions are determined through experiments, and the EBFC is successfully applied to power a low-power device.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Huayun Chen, Zhiheng You, Yuhui Hong, Xiao Wang, Mingming Zhao, Yushi Luan, Yibin Ying, Yixian Wang
Summary: This study developed a colorimetric sensor array using gas-responsive two-dimensional metal-organic framework (MOF) composites for highly sensitive detection of volatile organic compounds (VOCs). The dye/Zn-2(bim)(4) composites-based sensor arrays showed enhanced sensitivity and anti-interference properties. The findings demonstrate the potential use of dye/Zn-2(bim)(4) sensor arrays for early perception of plant diseases.
BIOSENSORS & BIOELECTRONICS
(2024)
