Article
Medicine, Research & Experimental
Shan Liu, Dong-sheng Xu, Min Li, Yang Zhang, Qi Li, Teng-teng Li, Li-qun Ren
Summary: This study revealed that icariin inhibits oxidized low-density lipoprotein-induced endothelial-mesenchymal transition by upregulating H19, thereby exerting a protective effect in atherosclerosis.
MOLECULAR THERAPY-NUCLEIC ACIDS
(2021)
Article
Immunology
Misagh Rajabinejad, Gelayol Asadi, Sedigheh Ranjbar, Fatemeh Rezaei Varmaziar, Mojgan Karimi, Farhad Salari, Ali Gorgin Karaji, Alireza Rezaiemanesh, Leila Afshar Hezarkhani
Summary: The MALAT1-H19/miR-19b-3p axis may be involved in the development of DN, and these molecules could be useful biomarkers for DN. Dysregulated expression of SEMA4C, PLXNB2, and ATG16L1, targeted by miR-19b-3p and miR-125a-5p, showed that they probably play a role in the development of DN.
IMMUNOLOGY LETTERS
(2022)
Article
Gastroenterology & Hepatology
Guillermo N. Lopez-Sanchez, Eduardo Montalvo-Jave, Mayra Dominguez-Perez, Barbara Antuna-Puente, Fredy O. Beltran-Anaya, Alfredo Hidalgo-Miranda, Norberto C. Chavez-Tapia, Misael Uribe, Natalia Nuno-Lambarri
Summary: The study investigated the correlation between liver hsa-miR-140-5p, hsa-miR-148-5p, and hsa-miR-122-3p and serum biomarkers CK-18, APOB, IL-6, IL-32, and TNF-a in MAFLD patients. The results showed significant differences in circulating levels of CK-18 in MAFLD patients, and a strong correlation was found between hsa-miR-122-3p, hsa-miR-140-5p, and hsa-miR-148b-5p versus the CAP value.
ANNALS OF HEPATOLOGY
(2022)
Article
Oncology
Wenkun Chen, Xuena Li, Bulin Du, Yan Cui, Yu Ma, Yaming Li
Summary: This study found that HOXA11-AS enhances proliferation and glycolysis in lung adenocarcinoma cells through the miR-148b-3p/PKM2 axis. These findings expand our understanding of the molecular mechanisms of lung adenocarcinoma tumorigenesis and glycolysis, and suggest that HOXA11-AS could serve as a diagnostic and prognostic marker for lung adenocarcinoma.
Article
Immunology
Sonia Kiran, Mousumi Mandal, Ahmed Rakib, Amandeep Bajwa, Udai P. Singh
Summary: This study investigates the role and mechanism of miR-10a-3p in adipose inflammation and adipogenesis using ex vivo and in vitro approaches. The findings suggest that miR-10a-3p mimic reduces the expression of inflammatory cells and cytokines, and induces the expression of FoxP3 in adipose tissue. In vitro experiments also show that miR-10a-3p mimic reduces the expression of inflammatory genes and lipid accumulation. This study provides a new opportunity for the development of miR-10a-3p as a novel therapeutic for adipose inflammation and its associated metabolic disorders.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Oncology
Hao Jin, Huan Wang, Xin Jin, Wenbo Wang
Summary: Osteosarcoma, a prevalent type of bone tumor, is prone to pulmonary metastasis, leading to high relapse risk for patients. The overexpression of H19 is significantly associated with the progression of osteosarcoma, influencing tumor cell phenotype by regulating microRNA-29a-3p and LIM and SH3 domain protein 1. This study may contribute to a better understanding of osteosarcoma pathogenesis.
Article
Cell Biology
Tao Zhou, Suining Li, Liehong Yang, Daokang Xiang
Summary: The study demonstrates that miR-363-3p negatively regulates NOX4 in coronary arterial endothelial cells (CAECs) through blocking the p38 MAPK signaling pathway, leading to reduced inflammation, oxidative stress, and apoptosis while improving cell viability. This protective mechanism of miR-363-3p against coronary heart disease (CHD) suggests it as a potential therapeutic target for CHD.
Article
Genetics & Heredity
Xiao-Yu Zhang, Ling Mao
Summary: This study identified the tumor-suppressive role of circ_0000442 in breast cancer for the first time and found PTEN as a novel direct target of miR-148b-3p. The regulatory role of circ_0000442/miR-148b-3p/PTEN/PI3K/Akt axis was preliminarily confirmed in breast cancer cells and mouse models. These findings provide important progress in understanding breast cancer and lay the foundation for further research on the function, diagnosis, therapy, and prognosis of circular RNAs in breast cancer.
Article
Cell Biology
Banyar Than Naing, Takami Takizawa, Takanobu Sakurai, Chaw Kyi-Tha-Thu, Toshihiro Takizawa
Summary: This study investigated the expression and distribution of lncRNA H19 and its derivative miRNA miR-675-3p in the mouse placenta during mid-to-late gestation using qPCR and ISH analyses. The results showed a trophoblast cell type-specific expression of lncRNA H19 and miR-675-3p during later stages of gestation. Furthermore, miR-675-3p was also found in H19-negative trophoblast cells, suggesting possible transfer between neighboring cells.
HISTOCHEMISTRY AND CELL BIOLOGY
(2023)
Article
Medicine, Research & Experimental
Jyotirmaya Behera, Anil Kumar, Michael J. Voor, Neetu Tyagi
Summary: The study found that BMMSC-Exo promotes osteogenesis and angiogenesis by transporting lnc-H19, which regulates the process through miR-106a and Angpt1. Results showed that BMMSC-Exo significantly enhanced endothelial angiogenesis and bone formation in vitro, and restored bone formation and mechanical quality in vivo.
Article
Medicine, General & Internal
Xinling Cao, Jingjing Zhang, Shadike Apaer, Gang Yao, Tao Li
Summary: This study investigates the combined effects and mechanisms of miR-19a-3p and miR-376c-3p in hepatocellular carcinoma (HCC) cells, demonstrating their activation of the Wnt/beta-catenin pathway via targeting SOX6 to promote biological functions of HCC cells.
INTERNATIONAL JOURNAL OF GENERAL MEDICINE
(2021)
Article
Oncology
Guang Shan, Xike Zhou, Juan Gu, Daoping Zhou, Wei Cheng, Huaiguo Wu, Yueping Wang, Tian Tang, Xuedong Wang
Summary: Exosomes derived from cancer-associated fibroblasts (CAFs) play a crucial role in promoting the metastasis, EMT, and drug resistance of bladder cancer cells through delivering miR-148b-3p. PTEN has been identified as a direct target of miR-148b-3p, and its overexpression can inhibit EMT, metastasis, and chemoresistance in bladder cancer cells. Downregulation of miR-148b-3p in CAF-derived exosomes may offer potential therapeutic opportunities for bladder cancer treatment by inhibiting the Wnt/beta-catenin pathway and promoting PTEN expression.
Article
Biochemistry & Molecular Biology
Jianwei Cao, Daibin Kuang, Ming Luo, Shanzhong Wang, Chunlai Fu
Summary: Acute lung injury (ALI) is a life-threatening disease caused by the severe response of the lungs to various insults. This study identifies the crucial role of circNCLN in lipopolysaccharide-induced ALI, as it acts as a molecular sponge to maintain the expression of TSLP in alveolar epithelial cells. Inhibition of circNCLN may serve as a potential treatment for ALI.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2022)
Article
Oncology
Xueshan Zhao, Xiaodong Jin, Qiuning Zhang, Ruifeng Liu, Hongtao Luo, Zhen Yang, Yichao Geng, Shuangwu Feng, Chengcheng Li, Lina Wang, Xiaohu Wang, Qiang Li
Summary: The lncRNA H19 plays a role in regulating radiosensitivity in NSCLC cells and may serve as a potential therapeutic target and impact the effectiveness of anti-tumor radiotherapy in NSCLC.
CANCER CELL INTERNATIONAL
(2021)
Article
Immunology
Changmin Wang, Ye Li, Yuhang Yi, Guiyu Liu, Ruojing Guo, Liyan Wang, Tian Lan, Wenjing Wang, Xiao Chen, Shihong Chen, Shu Yan Yu
Summary: Neuronal injury plays a critical role in the development of neurological and neuropsychiatric diseases, but the molecular mechanisms and potential therapeutic targets for preventing neuronal injury associated with brain function are not well characterized. This study demonstrates that deficiency of miR-26a-3p leads to microglial activation, increased levels of pro-inflammatory cytokines, and behavioral disorders in rats. These effects are mediated by the p38 MAPK-NF-kappa B signaling pathway. Inhibition of the p38 pathway ameliorates the abnormal behaviors and neuronal injury caused by miR-26a-3p knock-down. These findings suggest that miR-26a-3p acts as a critical factor in regulating neural activity and maintaining neuroplasticity, and targeting the p38 pathway may be a potential therapeutic strategy for neurological disorders.
JOURNAL OF NEUROINFLAMMATION
(2022)
Article
Biochemistry & Molecular Biology
Siva Santhiya Arul, Brinda Balakrishnan, Savithri S. Handanahal, Sangita Venkataraman
Summary: Viral nanoparticles are self-assembling delivery systems used for vaccines and therapeutic agents. They have improved biocompatibility and are widely used in various biomedical applications. However, large-scale production of VNPs still faces challenges.
Article
Biochemistry & Molecular Biology
Wisely Chua, Carl O. Marsh, Si En Poh, Winston LC. Koh, Melody Li Ying Lee, Li Fang Koh, Xin-Zi Emily Tang, Peter See, Zheng Ser, Shi Mei Wang, Radoslaw M. Sobota, Thomas L. Dawson Jr, Yik Weng Yew, Steven Thng, Anthony J. O'Donoghue, Hazel H. Oon, John E. Common, Hao Li
Summary: In this study, the gene expression of enzymes secreted by Malassezia globosa in the skin was investigated using targeted RNA sequencing. The study found that the expression of these enzymes is regulated by the fungus's environment and differs significantly from healthy skin sites. Additionally, a pseudoprotease MGL_3331 was identified to elicit an immune reaction in patients with atopic dermatitis. This highlights the importance of studying fungal proteins in physiologically relevant environments and their role in host immunity.
Article
Biochemistry & Molecular Biology
Belisa Parmeggiani, Marian Flores Signori, Cristiane Cecatto, Marina Rocha Frusciante, Manuela Bianchin Marcuzzo, Debora Guerini Souza, Rafael Teixeira Ribeiro, Bianca Seminotti, Diogo Onofre Gomes de Souza, Cesar Augusto Joa o Ribeiro, Moacir Wajner, Guilhian Leipnitz
Summary: Non ketotic hyperglycinemia (NKH) is a disorder caused by mutations in the genes encoding glycine cleavage system proteins, leading to severe neurodegeneration and white matter alterations. The study found that glycine affects myelin structure, glutamatergic system, and induces oxidative stress in neonatal rats and MO3.13 oligodendroglial cells.
Review
Biochemistry & Molecular Biology
Marie Rose Ndella Thiaw, Pascal Gantet
Summary: Mini zinc fingers play important roles in plant development, including control of cell division and expansion, meristem state transition, and growth and organ development. They have diverse modes of action, such as direct gene activation, interaction with specific transcription factors, and aggregation of protein complexes for chromatin remodeling. Further research is needed to explore their wider range of biological functions and their involvement in biotic and abiotic stress responses.
Article
Biochemistry & Molecular Biology
Shao-Jui Lai, Takahiro Kameda, Maasa Morita, Yuka Yamagata, Kaoruko Nishizaka, Yuna Horiuchi, Yukihiro Kobayashi, Yoko Usami, Jun-Jen Liu, Takeshi Kasama, Minoru Tozuka, Ryunosuke Ohkawa
Summary: In this study, the co-modification and impact of chymase and MPO on HDL were investigated. Sequential treatment with MPO and chymase generated two novel apoA-I fragments from HDL, one of which has a specific immunological property and can be used to predict patients with normal HDL-C levels and cardiovascular diseases.
Article
Biochemistry & Molecular Biology
Xiao-Mi Sun, Asami Yoshida, Takuya Ishii, Yan-Rong Jiang, Yi-Li Gao, Mikinori Ueno, Katsuya Hirasaka, Kiyoshi Osatomi
Summary: This study found that infection with the causative bacteria of edwardsiellosis, Edwardsiella tarda NUF251, leads to the production of superoxide radicals, causing oxidative stress. The research on the Jfsod1 gene suggests that the transcription factor NF-IL6 plays a significant role in upregulating the transcriptional activity of the gene in response to oxidative stress induced by NUF251 infection.
Article
Biochemistry & Molecular Biology
Shane M. Carey, Sean P. Kearns, Matthew E. Millington, Gregory S. Buechner, Beda E. Alvarez Jr, Leily Daneshian, Brendan Abiskaroon, Maksymilian Chruszcz, Edward L. D'Antonio
Summary: The importance of F337 in TcGlcK was investigated using the hole mutagenesis approach, revealing its key interactions with aromatic tail groups in inhibitor molecules. Mutants retaining activity and structural similarity were obtained, suggesting the possibility of TcHxK existing as a monomer lacking the F337 residue. Similar D-glucosamine inhibitors may bind less strongly to TcHxK due to the absence of van der Waals contacts from residue side chains.
Article
Biochemistry & Molecular Biology
Catia Barria, Diogo Athayde, Guillem Hernandez, Leonor Fonseca, Jorge Casinhas, Tiago N. Cordeiro, Margarida Archer, Cecilia M. Arraiano, Jose A. Brito, Rute G. Matos
Summary: Ribonucleases play a crucial role in RNA regulation by processing, degrading, and quality controlling cellular transcripts. Campylobacter jejuni, the main cause of human gastroenteritis, relies on the exoribonuclease PNPase (CjPNP) for low-temperature survival, virulence factor synthesis, and swimming, cell adhesion/invasion, and chick colonization abilities. The crystallographic structure of CjPNP, along with SAXS analysis, confirms its trimeric arrangement and provides insight into domain arrangement and flexibility. Mutations in highly conserved residues reveal unexpected RNA degradation activity even under conditions favoring polymerization. These findings have significant implications for the development of strategies to combat C. jejuni infections.
Article
Biochemistry & Molecular Biology
Elvira R. Shaykhutdinova, Maria S. Severyukhina, Inna V. Kholoshenko, Elena A. Gondarenko, Irina V. Shelukhina, Elena V. Kryukova, Alina M. Ismailova, Elena S. Sadovnikova, Igor A. Dyachenko, Arkady N. Murashev, Victor I. Tsetlin, Yuri N. Utkin
Summary: Evidence suggests that activating nicotinic acetylcholine receptors (nAChRs) can reduce cardiac injury caused by ischemia and reperfusion. In this study, the expression of a4, a6, and a132 nAChR subunits in the rat heart's left ventricle was demonstrated. The nAChR agonists cytisine and varenicline, commonly used for nicotine addiction treatment, were found to significantly reduce myocardial ischemia-reperfusion injury, with varenicline showing greater protection. The presence of a4132, a7, and/or a6132-containing receptors suggests that the protective effect of varenicline may be mediated by these subunits. These findings indicate a potential new use for cytisine and varenicline as cardioprotective agents.
Article
Biochemistry & Molecular Biology
Xinhai Xiong, Wenjie Chen, Cheng Chen, Qi Wu, Chaopeng He
Summary: This article summarizes the important roles of Cx43 in disease development from the perspective of subcellular localization and provides new ideas for Cx43 as a therapeutic target and the search for related pathological mechanisms.
Article
Biochemistry & Molecular Biology
Veronica Bastos, Simone Pascoal, Kamila Lopes, Marcia Mortari, Helena Oliveira
Summary: Cancer, especially melanoma, is a major global health problem due to its metastatic capacity, treatment resistance, and high mortality rates. The Chartergellus-CP1 peptide isolated from Chartergellus communis wasp venom showed selective cytotoxicity towards melanoma cell lines, inducing apoptosis, cell cycle impairment, and intracellular ROS increase.
Article
Biochemistry & Molecular Biology
Charlotte Claeyssen, Nathan Bulangalire, Bruno Bastide, Onnik Agbulut, Caroline Cieniewski-Bernard
Summary: Maintenance of striated muscle tissue relies on a dynamic network of protein-protein interactions. Desmin intermediate filaments play a crucial role in heart and skeletal muscle by maintaining cell structure and contributing to various cellular processes. The assembly of desmin intermediate filaments requires aB-crystallin, which is involved in multiple cellular functions.
Article
Biochemistry & Molecular Biology
Dhritiman Dey, Adhiraj Dasgupta, Dipanjan Ghosh, Oindrila Bhattacharjee, Abhrajyoti Ghosh, Ayae Honda, Dhrubajyoti Chattopadhyay
Summary: This study successfully identified host factors that interact with Chandipura Virus (CHPV) and play a critical role in infection, including A2M, LRP1, and GRP78. Knocking out A2M has a severe effect on viral infection, indicating the potential role of these host proteins in viral entry into host cells.
Article
Biochemistry & Molecular Biology
Anna Dotsenko, Igor Sinelnikov, Aleksandra Rozhkova, Ivan Zorov, Arkady Sinitsyn
Summary: Xylanases play important roles in various industrial applications, such as feed additives, pulp and paper bleaching, and the production of bread, food, and drinks. Xylanases from the GH10 family show high thermostability and resistance to protein inhibitors, making them promising candidates for these processes. This study successfully improved the thermostability and enzymatic activity of GH10 xylanase, which could enhance its effectiveness as a feed additive and during pulp and paper bleaching.
Article
Biochemistry & Molecular Biology
M. Jaquet, M. Bengue, K. Lambert, G. Carnac, D. Misse, C. Bisbal
Summary: Changes to our environment have led to the emergence of human pathogens such as chikungunya virus, which is now a major public health concern. In this study, the interconnection between skeletal muscle cells metabolism, their differentiation stage, and the infectivity of chikungunya virus was investigated. The results showed that chikungunya virus infectivity is dependent on cell differentiation and metabolism and that the virus interferes with cellular metabolism.