期刊
BIOCHEMICAL ENGINEERING JOURNAL
卷 145, 期 -, 页码 127-136出版社
ELSEVIER
DOI: 10.1016/j.bej.2019.02.020
关键词
Heterotrophic growth; Non-axenic culture; Microalgae; Bacteria contamination; Wastewaters; Tetradesmus obliquus
资金
- Bio-P S.r.l. (Rome, Italy)
A fed-batch strategy is proposed to produce microalgae biomass under non-axenic heterotrophic conditions. The strategy induces the alternation of N-deplete (Glucose-replete) and N-replete (Glucose-deplete) cultivation phases by the periodic and uncoupled supply of glucose and NO3- to the culture. Cultivation of the microalga T. obliquus with this strategy reduced the ratio of the bacteria to microalgae cell concentration from 1.6, attained by conventional photoautotrophic cultivation, to 0.03. During the N-deplete phase, microalgae duplication stopped and biomass concentration increased 1.9 times, while during the N-replete phase, microalgae duplicated halving their average size and losing about 25% of their weight. The process proved to be effective under several consecutive cycles. Biomass productivity until 6.1 g/Ld and biomass concentration until 26 g/L were achieved. The results demonstrate that the proposed strategy can effectively prevent bacterial contamination, paving the way to the large scale production of microalgae biomass under non-axenic heterotrophic conditions.
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