期刊
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS
卷 667, 期 -, 页码 14-21出版社
ELSEVIER SCIENCE INC
DOI: 10.1016/j.abb.2019.04.003
关键词
Matrix vesicles; Nucleation core; Mineralization; Elastic modulus; Atomic force microscopy
资金
- grant NanOArt from the University of Rome Tor Vergata
- National Institute of Dental and Craniofacial Research (NIDCR) [DE128890]
- National Institute of Biomedical Imaging and Bioengineering (NIBIB) [T32EB009380]
- FAPESP [2016/21236-0, 2014/11941-3]
- CNPq [167497/2017-0]
- Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior - Brasil (CAPES) [001]
- Polish Academy of Sciences
- Government of the Republic of Poland [1/A/1]
- Government of the Republic of France POLONIUM 2018/2019 [1/A/1]
- Polish National Science Centre [2016/23/N/NZ4/03313]
- BBSRC [BBS/E/D/10002071] Funding Source: UKRI
Matrix vesicles (MVs) are a class of extracellular vesicles that initiate mineralization in cartilage, bone, and other vertebrate tissues by accumulating calcium ions (Ca2+) and inorganic phosphate (P-i) within their lumen and forming a nucleation core (NC). After further sequestration of Ca2+ and P-i, the NC transforms into crystalline complexes. Direct evidence of the existence of the NC and its maturation have been provided solely by analyses of dried samples. We isolated MVs from chicken embryo cartilage and used atomic force microscopy peak force quantitative nanomechanical property mapping (AFM-PFQNM) to measure the nanomechanical and morphological properties of individual MVs under both mineralizing (+Ca2+) and non-mineralizing (-Ca2+) fluid conditions. The elastic modulus of MVs significantly increased by 4-fold after incubation in mineralization buffer. From AFM mapping data, we inferred the morphological changes of MVs as mineralization progresses: prior to mineralization, a punctate feature, the NC, is present within MVs and this feature grows and stiffens during mineralization until it occupies most of the MV lumen. Dynamic light scattering showed a significant increase in hydrodynamic diameter and no change in the zeta potential of hydrated MVs after incubation with Ca2+. This validates that crystalline complexes, which are strongly negative relative to MVs, were forming within the lumen of MVs. These data were substantiated by transmission electron microscopy energy dispersive X-ray and Fourier transform infrared spectroscopic analyses of dried MVs, which provide evidence that the complexes increased in size, crystallinity, and Ca/P ratio within MVs during the mineralization process.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据