Article
Surgery
Carlos S. Cabalag, Michael Yates, Mariana Benitez Corrales, Paul Yeh, Stephen Q. Wong, Bonnie Z. Zhang, Kenji M. Fujihara, Lynn Chong, Michael W. Hii, Sarah-Jane Dawson, Wayne A. Phillips, Cuong P. Duong, Nicholas J. Clemons
Summary: The study demonstrates the clinical utility of ctDNA in predicting disease status and treatment response in esophageal adenocarcinoma patients. It shows that ctDNA can provide additional prognostication over conventional staging investigations and may be a useful biomarker for surveillance of recurrent disease.
Review
Medicine, General & Internal
Nancy Huang, Katie J. Lee, Mitchell S. Stark
Summary: Melanoma is the leading cause of skin cancer deaths, and liquid biomarkers have advantages in monitoring tumor burden and heterogeneity. The only serological marker currently used, lactate dehydrogenase, is not specific or sensitive enough. This review summarizes the current research on liquid biomarkers, including circulating tumor cells, circulating tumor DNA, microRNA, and extracellular vesicles.
FRONTIERS IN MEDICINE
(2022)
Article
Ophthalmology
Jasmine H. Francis, Christopher A. Barker, A. Rose Brannon, Julia Canestraro, Melissa Robbins, Christina E. Swartzwelder, Sara Levine, Crystal Law, Michael F. Berger, Alexander Shoushtari, David H. Abramson
Summary: This study aims to investigate the presence of plasma circulating tumor DNA (ctDNA) in patients with uveal melanoma during and after primary tumor treatment. The study found that ctDNA was significantly more detectable at 48 to 72 hours after plaque brachytherapy compared with less than 48 hours, and ctDNA can also be detected during enucleation. Relative increases in ctDNA levels may herald the development of clinically apparent metastases.
INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE
(2022)
Article
Multidisciplinary Sciences
Kunwar Somesh Vikramdeo, Shashi Anand, Mohammad Aslam Khan, Moh'd Khushman, Martin J. Heslin, Seema Singh, Ajay Pratap Singh, Santanu Dasgupta
Summary: The study demonstrates that mitochondrial DNA (mtDNA) mutations in circulating extracellular vesicles (EVs) can be used for reliable diagnosis of early pancreatic ductal adenocarcinoma (PDAC).
SCIENTIFIC REPORTS
(2022)
Article
Genetics & Heredity
Bing Pei, Guodong Zhao, Zhixin Geng, Yue Wang, Menglin Wang, Xiaomei Wang, Shangmin Xiong, Minxue Zheng
Summary: Esophageal cancer is a leading cause of cancer-related deaths in China, but there is still a lack of low-cost, efficient, and accurate non-invasive methods for early detection.
FRONTIERS IN GENETICS
(2023)
Article
Chemistry, Analytical
Pablo J. Dopico, Minh-Chau N. Le, Benjamin Burgess, Zhijie Yang, Yu Zhao, Youxiang Wang, Thomas J. George, Z. Hugh Fan
Summary: While patients with resectable pancreatic ductal adenocarcinoma (PDAC) show improved survival, their survival remains low due to occult metastatic disease and treatment resistance. This study tracked circulating biomarkers in resectable PDAC patients throughout treatment and found that the majority of these biomarkers were detected after the start of neoadjuvant therapy but before surgery.
Review
Oncology
Zuyang Yuan, Xinfeng Wang, Xiao Geng, Yin Li, Juwei Mu, Fengwei Tan, Qi Xue, Shugeng Gao, Jie He
Summary: Esophageal cancer is a common global health issue with late diagnosis and lack of effective therapy. Liquid biopsies, specifically cfDNA, show promise in non-invasive cancer diagnosis and patient monitoring. However, research on the use of cfDNA in esophageal cancer is limited.
CANCER COMMUNICATIONS
(2021)
Article
Oncology
Yang Song, Suzhu Zhu, Ning Zhang, Lixin Cheng
Summary: A diagnostic signature consisting of four miRNA pairs was developed for early detection of esophageal cancer. The signature was validated in two independent datasets, demonstrating high performance with accuracy in diagnosing early-stage esophageal cancer.
FRONTIERS IN ONCOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Xinxing Miao, Qianqian Fang, Xiang Xiao, Sidi Liu, Renfei Wu, Jun Yan, Baoqing Nie, Jian Liu
Summary: This study presents a new method integrating cycled enzymatic DNA amplification and Au nanoparticle@silicon-assisted surface-enhanced Raman scattering (SERS) technique for sensitive detection of single-base-mutated ctDNA sequences in diluted blood samples. The approach can be applied in translational medicine for early cancer diagnosis, therapeutic effect monitoring, and prognosis of patients.
FRONTIERS IN MOLECULAR BIOSCIENCES
(2021)
Article
Oncology
Brice Pastor, Thierry Andre, Julie Henriques, Isabelle Trouilloud, Christophe Tournigand, Marine Jary, Thibault Mazard, Christophe Louvet, Simon Azan, Audrey Bauer, Benoit Roch, Cynthia Sanchez, Dewi Vernerey, Alain R. Thierry, Antoine Adenis
Summary: This study demonstrates that pretreatment levels of cfDNA and mutant ctDNA may identify mCRC patients who could benefit from regorafenib treatment.
MOLECULAR ONCOLOGY
(2021)
Article
Multidisciplinary Sciences
Weilin Peng, Guangxu Tu, Zhenyu Zhao, Boxue He, Qidong Cai, Pengfei Zhang, Xiong Peng, Shuai Shi, Xiang Wang
Summary: This study identified a diagnostic model involving methylation of four genes for early diagnosis of EAC by analyzing DNA methylome and gene expression data from EAC tissues. The model was validated in other datasets and may have potential applications in prospective EAC cases.
Article
Oncology
Kajsa E. Affolter, Sabine Hellwig, David A. Nix, Mary P. Bronner, Alun Thomas, Carrie L. Fuertes, Cindy L. Hamil, Ignacio Garrido-Laguna, Courtney L. Scaife, Sean J. Mulvihill, Hunter R. Underhill
Summary: Utilizing a panel of 118 genes and technical replicates of ccfDNA to eliminate NGS-related errors, this study successfully enhanced ctDNA detection from PDAC patients. Pre-operative detection of ctDNA, guided by mutations present in tumor DNA, was associated with shorter survival, suggesting its potential as a non-invasive prognostic biomarker.
Article
Oncology
Chandan Bhambhani, Erin Sandford, Catherine T. Haring, Collin Brummel, Kirsten L. Tuck, Mary Olesnavich, Apurva D. Bhangale, Heather M. Walline, Sarah M. Dermody, Matthew E. Spector, Steven B. Chinn, Keith Casper, Michelle Mierzwa, Paul L. Swiecicki, J. Chad Brenner, Muneesh Tewari
Summary: A high-performance ddPCR assay called CHAMP-16 was developed to enhance the detection of HPV ctDNA in plasma from patients with HPV+ OPSCC. The assay targets nine regions of the HPV16 genome and showed significantly higher HPV16 counts compared to previous assays. The CHAMP-16 assay has the potential to detect recurrences earlier and is cost-effective for population screening and post-treatment surveillance.
Article
Oncology
Tao Liu, Qianqian Yao, Hai Jin
Summary: Esophageal squamous cell carcinoma (ESCC) is a lethal cancer with high recurrence rates and early detection challenges. Circulating tumor DNA (ctDNA) is emerging as a useful biomarker for non-invasive diagnosis and monitoring tumor prognosis. Analysis of plasma cell-free DNA (cfDNA) variations in ESCC patients revealed correlation between postoperative cfDNA detection and shorter overall survival as well as higher relapse rates.
FRONTIERS IN ONCOLOGY
(2021)
Article
Oncology
Yingying Cao, Rui Zhao, Kai Guo, Shuai Ren, Yaping Zhang, Zipeng Lu, Lei Tian, Tao Li, Xiao Chen, Zhongqiu Wang
Summary: This study developed a novel metabolic model using both serum and tissue samples for early detection of stage-I pancreatic ductal adenocarcinoma (PDAC). The model showed high diagnostic accuracy and outperformed the traditional CA19-9 marker.
FRONTIERS IN ONCOLOGY
(2022)
Article
Oncology
Qing Zhou, Simon P. Gampenrieder, Sophie Frantal, Gabriel Rinnerthaler, Christian F. Singer, Daniel Egle, Georg Pfeiler, Rupert Bartsch, Viktor Wette, Angelika Pichler, Edgar Petru, Peter C. Dubsky, Zsuzsanna Bago-Horvath, Christian Fesl, Margaretha Rudas, Anders Stahlberg, Ricarda Graf, Sabrina Weber, Nadia Dandachi, Martin Filipits, Michael Gnant, Marija Balic, Ellen Heitzer
Summary: The detection and persistence of ctDNA in plasma can predict the response to neoadjuvant treatment and identify patients who will not achieve complete pathological response or be classified with residual cancer burden II/III.
CLINICAL CANCER RESEARCH
(2022)
Article
Oncology
Malin Linden, Christoffer Vannas, Tobias Osterlund, Lisa Andersson, Ayman Osman, Mandy Escobar, Henrik Fagman, Anders Stahlberg, Pierre Aman
Summary: FET fusion oncoproteins bind with SWI/SNF complexes and indirectly interact with BRD4, co-localizing on chromatin and interacting with transcription factors and RNA Polymerase II.
MOLECULAR ONCOLOGY
(2022)
Article
Oncology
Soheila Dolatabadi, Emma Jonasson, Lisa Andersson, Manuel Luna Santamaria, Malin Linden, Tobias Osterlund, Pierre Aman, Anders Stahlberg
Summary: Myxoid liposarcoma, a type of sarcoma characterized by FET fusion oncogenes, is associated with JAK-STAT signaling. In this study, the researchers found that the FET fusion oncogene FUS-DDIT3 is closely connected to JAK-STAT signaling. They identified genes regulated by both FUS-DDIT3 and JAK1/2 inhibition, and confirmed the targets using chromatin immunoprecipitation sequencing data sets. The findings provide new insights into the molecular mechanisms underlying tumor progression and potential avenues for targeted therapies.
FRONTIERS IN ONCOLOGY
(2022)
Article
Medicine, Research & Experimental
Simona Salerno, Anders Stahlberg, Andre Holdfeldt, Elinor Bexe Lindskog, Goran Landberg
Summary: Colorectal cancer is a common diagnosis worldwide, with many patients not responding to standard chemotherapy. This study used patient-derived scaffolds to investigate how individual patient-specific microenvironments influence the chemotherapy response in colorectal cancer. The findings suggest that tumor microenvironment can influence drug resistance and potential disease relapse, providing insights for optimized precision medicine in colorectal cancer treatment.
JOURNAL OF TRANSLATIONAL MEDICINE
(2022)
Article
Immunology
Peter Micallef, Milica Vujicic, Yanling Wu, Eduard Peris, Ying Wang, Belen Chanclon, Anders Stahlberg, Susanna L. Cardell, Ingrid Wernstedt Asterholm
Summary: Adipose tissue undergoes remodeling during weight gain and tumor growth. C1qtnf3 is highly expressed in tumor-associated inguinal adipose tissue of obese mice. It chemotactically attracts macrophages and may influence adipose tissue through regulating macrophage polarization.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Oncology
Elena Garre, Anna Gustafsson, Maria Carmen Leiva, Joakim Hakansson, Anders Stahlberg, Aniko Kovacs, Goran Landberg
Summary: In this study, the activity of the cancer microenvironment in breast cancer patients was monitored using patient-derived scaffolds. The data showed that changes in gene expression induced by the scaffolds were linked to clinical and prognostic properties of the original cancer. The results suggest that patient-derived scaffolds can serve as a complementary diagnostic tool for breast cancer.
Article
Biochemistry & Molecular Biology
Christoffer Vannas, Lisa Andersson, Soheila Dolatabadi, Parmida Ranji, Malin Linden, Emma Jonasson, Anders Stahlberg, Henrik Fagman, Pierre Aman
Summary: Research has shown that different HSP90 inhibitors can have significant differences in therapeutic effects and outcomes for myxoid liposarcoma, with some inhibitors even leading to paradoxical effects. Careful consideration should be given to drug selection and treatment strategies.
Article
Medical Laboratory Technology
Tobias Osterlund, Stefan Filges, Gustav Johansson, Anders Stahlberg
Summary: The study developed a new bioinformatics pipeline, UMIErrorCorrect, for analyzing sequencing data containing UMIs, and provided a user interface called UMIAnalyzer. Analysis of data demonstrated that UMIErrorCorrect showed higher sensitivity in variant detection for targeted UMI sequencing data.
CLINICAL CHEMISTRY
(2022)
Article
Oncology
Ayman Osman, Malin Linden, Tobias Osterlund, Christoffer Vannas, Lisa Andersson, Mandy Escobar, Anders Stahlberg, Pierre Aman
Summary: DDIT3 is a transcription factor that plays a key role in cellular stress responses, cell cycle regulation, apoptosis, cell differentiation, and cancer development. It can bind to DNA and regulate target genes by forming heterodimers with other bZIP transcription factors. DDIT3 mainly binds to the promoter region of regulated genes and shows a strong overlap with H3K27 acetylation marks.
EXPERIMENTAL CELL RESEARCH
(2023)
Article
Biology
Serik Sagitov, Anders Stahlberg
Summary: This study utilizes molecular barcoding technology to detect extremely rare variant alleles, such as tumor DNA, within a complex mixture of DNA molecules, in order to avoid sequencing errors. They propose a random model to describe the random outcome of PCR barcoding and discover a remarkable asymptotic pattern under moderate PCR cycle numbers. The results of this model will assist users in drawing more accurate conclusions from the outcomes of different sequencing protocols.
JOURNAL OF THEORETICAL BIOLOGY
(2023)
Article
Cell Biology
Rossella Crescitelli, Stefan Filges, Nasibeh Karimi, Ornella Urzi, Tamara Alonso-Agudo, Anders Stahlberg, Jan Lotvall, Cecilia Lasser, Roger Olofsson Bagge
Summary: This study identified tumor-specific DNA mutations in EVs isolated from melanoma metastatic tissues, showing a higher mutant allele frequency in DNA isolated from tumor-derived EVs compared to total plasma DNA, indicating the potential role of tumor EVs as future biomarkers in melanoma.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Chemistry, Analytical
Amin Forootan, Daniel Andersson, Soheila Dolatabadi, David Svec, Jose Andrade, Anders Stahlberg
Summary: This study used self-organizing maps technology to analyze cells from myxoid liposarcoma and Ewing sarcoma, and found that they have similar gene expression signatures, indicating that these two tumors exhibit similar cellular characteristics.
Review
Biotechnology & Applied Microbiology
Eva Pasquier, Jennifer Rosendahl, Amalie Solberg, Anders Stahlberg, Joakim Hakansson, Gary Chinga-Carrasco
Summary: Breast cancer is a common cancer among women, and the efficiency of available treatments varies. Current 2D models overestimate drug efficiency, leading to higher failure rates in later clinical trials. Therefore, new model systems are needed for drug screening. 3D printed hydrogels with active components provide interesting candidates for next generation cancer cell models.
BIOENGINEERING-BASEL
(2023)
Article
Multidisciplinary Sciences
Elin Schoultz, Shawn Liang, Therese Carlsson, Stefan Filges, Anders Stahlberg, Henrik Fagman, Clotilde Wiel, Volkan Sayin, Mikael Nilsson
Summary: It is found that tumor development and progression differ significantly in lung and thyroid targeted by Braf mutation. Even with similar levels of Braf mutation in both tissues, non-induced mutants only developed multiple lung adenocarcinomas, while thyroid tumors were rare and constrained. This indicates that BRAF(V600E)-induced tumorigenesis follows organ-specific traits that might be modified differently by a shared lineage factor.
Article
Oncology
Vasu R. Sah, Henrik Jespersen, Joakim Karlsson, Lisa M. Nilsson, Mattias Bergqvist, Iva Johansson, Ana Carneiro, Hildur Helgadottir, Max Levin, Gustav Ullenhag, Anders Stahlberg, Roger Olofsson Bagge, Jonas A. Nilsson, Lars Ny
Summary: The 2-year follow-up of the PEMDAC trial showed that patients with high levels of CCL21 in blood had better treatment response and survival. The expression of CCL21 in tumors and the presence of TLS-like regions were also associated with longer survival.
CANCER RESEARCH COMMUNICATIONS
(2023)
Article
Cardiac & Cardiovascular Systems
Soh Hosoba, Toshiaki Ito, Makoto Mori, Riku Kato, Koh Kajiyama, Shogo Maeda, Yuji Nakai, Yoshihiro Morishita
Summary: This study describes the approach and perioperative outcomes of totally endoscopic isolated and concomitant surgical aortic valve replacement (AVR) using various valve types. The results demonstrate that endoscopic AVR can safely address concomitant valve diseases.
ANNALS OF THORACIC SURGERY
(2024)