期刊
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
卷 58, 期 27, 页码 9068-9072出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/anie.201903134
关键词
antibodies; bioconjugation; cyclic peptides; DNA; nanostructure
资金
- Innovation Fund Denmark
- Novo Nordisk Foundation (CEMBID) [NNF17OC0028070]
- Danish National Research Foundation (CDNA)
- Danish National Research Foundation (CellPat)
The development of methods for conjugation of DNA to proteins is of high relevance for the integration of protein function and DNA structures. Here, we demonstrate that protein-binding peptides can direct a DNA-templated reaction, selectively furnishing DNA-protein conjugates with one DNA label. Quantitative conversion of oligonucleotides is achieved at low stoichiometries and the reaction can be performed in complex biological matrixes, such as cell lysates. Further, we have used a star-like pentameric DNA nanostructure to assemble five DNA-Rituximab conjugates, made by our reported method, into a pseudo-IgM antibody structure that was subsequently characterized by negative-stain transmission electron microscopy (nsTEM) analysis.
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