Review
Chemistry, Analytical
Kaiser Parnamets, Tamas Pardy, Ants Koel, Toomas Rang, Ott Scheler, Yannick Le Moullec, Fariha Afrin
Summary: High-throughput microflow cytometry, particularly droplet microflow cytometry (DMFC), enables cell analysis by isolating each cell within a droplet as a microreactor. However, challenges arise when applying commonly used fluorometry and fluorescent microscopy techniques to this novel methodology.
Review
Materials Science, Multidisciplinary
Mariadel Mar Gonzalez del Campo, Andreu Vaquer, Roberto de la Rica
Summary: In recent years, paper has become a widely used material for developing various types of sensors, and a polymer-based toolbox has emerged to enhance the functionalities of these devices. This article compiles examples from recent literature on microfluidic systems and polymer-based transducers implemented in paper-based analytical platforms. The use of printing methods to pattern polymers allows for mass production of these advanced paper-based devices without requiring specialized facilities.
ADVANCED MATERIALS TECHNOLOGIES
(2022)
Article
Materials Science, Multidisciplinary
Zhiqiang Zhu, Tianao Chen, Yuanqing Zhu, Fangsheng Huang, Kai Mu, Ting Si, Ronald X. Xu
Summary: Programmable pulsed aerodynamic printing (PPAP) is a method for patterning soft materials with extensive compatibility and multi-scale and multi-interface properties. PPAP utilizes programmable pulsed airflow to shear pendant droplets at the nozzle orifice, covering a broad range of parameters. Demonstrations of PPAP include cell-loaded annular particles, liquid metal circuits, laser stimuli-responsive microcapsules, calcium alginate elastomers, and 3D multi-interface clusters.
Article
Chemistry, Multidisciplinary
Shaobo Jin, Guoyong Ye, Na Cao, Xuling Liu, Liguo Dai, Pengpeng Wang, Tong Wang, Xueyong Wei
Summary: This study demonstrates a method of surface acoustic wave-controlled fusion of microdroplets, which shows good arrangement effect of different sizes of microdroplets without any modification.
Review
Chemistry, Analytical
Joao Flavio da Silveira Petruci, Diandra Nunes Barreto, Mariana A. Dias, Erika P. Felix, Arnaldo A. Cardoso
Summary: Ground-level ozone is increasing worldwide due to anthropogenic emissions and the use of ozone generators. This poses risks to public health, indoor air quality, and materials. Monitoring and control policies are essential, but determining ozone gas remains a challenge. Advances in measurement techniques have been made, but further exploration is needed for device construction and components active towards ozone.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2022)
Review
Chemistry, Analytical
Meritxell Rovira, Cesar Fernandez-Sanchez, Cecilia Jimenez-Jorquera
Summary: The development of diagnostic tools for measuring a wide spectrum of target analytes has grown significantly in the last decades. Wearable devices that can provide real-time analysis of biofluids, particularly sweat, have gained attention for various applications. While current devices mainly offer discrete qualitative results, there is a high demand for quantitative biomarker analysis, leading to research on fluidic elements and sensor architectures.
Article
Chemistry, Analytical
Junyi Chen, Shaoqi Huang, Yan Long, Kan Wang, Yangtai Guan, Lianping Hou, Bo Dai, Songlin Zhuang, Dawei Zhang
Summary: Droplet-based microfluidics has great potential in various applications, and this paper introduces a modular microfluidic system using 3D printing technology for droplet generation. The system allows flexible control of droplet size and generation rate by adjusting flow rates. It is a promising candidate for chemical and biological applications.
Review
Chemistry, Analytical
Can Huang, Yuqian Jiang, Yuwen Li, Han Zhang
Summary: Since its invention, droplet microfluidic technologies have been widely used for high-throughput chemical and biological functional screening applications, and have undergone significant improvement and study. Each droplet serves as an individual bioreactor, enabling the screening of millions of droplets individually based on specific requirements. This paper reviews the current methods for droplet detection and active sorting in microfluidic systems, providing an analysis of the strengths and weaknesses of each technology, and concludes with future development prospects.
Article
Chemistry, Analytical
Beiyu Hu, Shun Ye, Dongwei Chen, Bingliang Xie, Ran Hu, Yuxin Qiao, Yanghuan Yu, Haiyan Yu, Xu Zheng, Ying Lan, Wenbin Du
Summary: Conventional picoinjectors face difficulties in eliminating cross-contamination between droplets, while stepinjection effectively increases the pressure difference by elevating the diffusional buffer zone into the main channel, successfully avoiding cross-contamination and enabling widespread use in contamination-sensitive enzymatic assays.
ANALYTICAL CHEMISTRY
(2021)
Article
Chemistry, Physical
Linshan Wu, Zhiguang Guo, Weimin Liu
Summary: This article discusses the development of microfluidic technology and the importance of droplet control. The balance between droplet driving force and fluid resistance is crucial for achieving selective droplet transport. The article also explores the effects of external fields on droplet transport and the applications and challenges of microfluidics in various fields.
ADVANCES IN COLLOID AND INTERFACE SCIENCE
(2022)
Review
Biophysics
Lexiang Zhang, Rokshana Parvin, Mingshuo Chen, Dingmeng Hu, Qihui Fan, Fangfu Ye
Summary: Droplet microfluidic technology has revolutionized biomolecular analytical research by allowing visualization, barcoding, and analysis of single cells and single molecules in picolitre droplets. This review focuses on diverse screening applications of droplet microfluidic technology, including genomic data analysis, drug susceptibility testing, cancer subtype identification, virus-host interactions, and large-scale combinational screening.
BIOSENSORS & BIOELECTRONICS
(2023)
Review
Chemistry, Multidisciplinary
Muhammad Adeel, Kanwal Asif, Md. Mahbubur Rahman, Salvatore Daniele, Vincenzo Canzonieri, Flavio Rizzolio
Summary: Advancements in nanomaterials have led to the development of new generations of glucose sensors, with metal-organic frameworks (MOFs) providing a basis for constructing glucose sensing devices. This review comprehensively summarizes the characteristics of MOFs and their application in glucose detection devices, highlighting working principles and current challenges in the field.
ADVANCED FUNCTIONAL MATERIALS
(2021)
Article
Chemistry, Analytical
Kailiang Zhang, Fangyuan Xing, Jiuqing Liu, Zhijie Xie
Summary: This paper presents a method for effective on-chip droplet generation, splitting, and switching using controllable hydrodynamics. A two-dimensional simulation model based on the phase-field method is established to analyze the hydrodynamic droplet manipulation behaviors. The numerical results show that the droplets can be generated with controlled sizes and can realize on-demand splitting and switching.
ANALYTICA CHIMICA ACTA
(2022)
Article
Chemistry, Multidisciplinary
Yunxing Lu, Zhaoduo Tong, Zhenhua Wu, Xiaoyu Jian, Lin Zhou, Shihui Qiu, Chuanjie Shen, Hao Yin, Hongju Mao
Summary: Exosomes, as a unique biomarker, have potential applications in cancer diagnostics and prognosis. This study presents an integrated microfluidic system for on-chip isolation of exosomes and RNA analysis of lung cancer using droplet digital PCR. The experiments demonstrate the system's high linearity and sensitivity, making it a promising tool for early cancer diagnosis and prognosis.
CHINESE CHEMICAL LETTERS
(2022)
Article
Chemistry, Multidisciplinary
Lidia A. Perez-Marquez, Marcelle D. Perretti, Raul Garcia-Rodriguez, Fernando Lahoz, Romen Carrillo
Summary: 3-Nitrotyrosine (NT) is an important biomarker of renal damage, but lacks an appropriate sensing method for clinical diagnosis. This study presents a fluorescent supramolecular sensor that selectively detects NT in human blood serum, providing a sensitive detection limit within physiological concentrations observed in chronic kidney disease.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2022)
Article
Biochemistry & Molecular Biology
Arti B. Dumbrepatil, Soumi Ghosh, Kelcie A. Zegalia, Paige A. Malec, J. Damon Hoff, Robert T. Kennedy, E. Neil G. Marsh
JOURNAL OF BIOLOGICAL CHEMISTRY
(2019)
Article
Chemistry, Multidisciplinary
Meng Sun, Zhengda Li, Qiong Yang
JOURNAL OF CHEMICAL EDUCATION
(2019)
Article
Nanoscience & Nanotechnology
Qi Zhang, Xuewei Wang, Vanessa Decker, Mark E. Meyerhoff
ACS APPLIED MATERIALS & INTERFACES
(2020)
Article
Chemistry, Analytical
Qi Zhang, Griffin P. Murray, Joseph E. Hill, Stephen L. Harvey, Alvaro Rojas-Pena, Jonathan Choi, Yang Zhou, Robert H. Bartlett, Mark E. Meyerhoff
ANALYTICAL CHEMISTRY
(2020)
Article
Biochemical Research Methods
Meng Sun, Gembu Maryu, Shiyuan Wang, Qiong Yang, Ryan C. Bailey
Summary: This unique microfluidic technique utilizes a membrane filter and plug-in tubes to remove oil and pack water-in-oil droplets for controlled incubation of droplet-based assays. The system allows for extended and controllable incubation of droplets in microchannels, and can be easily integrated into most droplet-generation devices without altering their original designs. Additionally, the technique is evaluated and confirmed to be efficient for time-dependent enzyme assays with a fluorometric readout, and can be generalized to control inter-droplet distance for studying droplet communication and pattern formation.
Review
Chemistry, Analytical
Qi Zhang, Mark E. Meyerhoff
Summary: This review introduces the physiological functions of NO to improve the biocompatibility of implantable electrochemical sensors, as well as provides a detailed summary of various types of NO releasing sensors and their performance in benchtop and/or in vivo testing. Discussions on prospects of future developments in NO releasing sensor technology for clinical use are also presented.
Article
Polymer Science
Yang Zhou, Peixuan Wu, Jianfeng Wu, Joshua C. Doverspike, Qi Zhang, Jinyu Shao, Chuanwu Xi, Yuanyuan Liu, Mark E. Meyerhoff
Summary: This study reported the preparation of NO-releasing poly(n-butyl methacrylate) films by incorporating S-nitroso-N-acetylpenicillamine, and investigated the NO-releasing profiles of such films under physiological conditions. The results showed that the PBMA films doped with SNAP exhibited good NO-releasing performance with antimicrobial and anti-biofilm properties against bacteria. This controllable NO release using PBMA films could be beneficial for therapeutic or biomedical device applications.
Article
Cell Biology
Megan M. Nelson, J. Damon Hoff, Mya L. Zeese, Gabriel Corfas
Summary: PARP1 plays a novel role in brain development by regulating Cajal-Retzius (CR) cells, neuronal density, and cell adhesion.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Chemistry, Multidisciplinary
Brock Brady, Wuwei Li, Nashwan Farooque, Christopher Ehrhardt, Mark E. Meyerho, Xuewei Wang
Summary: This article introduces a urinary catheter-based sensor for detecting the most common E. coli bacteria in urinary tract infections associated with the use of urinary catheters. By incorporating a nitrosating agent and indole into a silicone catheter, a red dimeric product called indoxyl red is generated, allowing for highly sensitive and specific color-based indole sensing. The method can be applied in real catheters to detect indole and E. coli in urine.
Article
Chemistry, Multidisciplinary
Alex Wolf, Mohsen Tabasi, Mark Zacharek, Glenn Martin, Marc B. Hershenson, Mark E. Meyerhoff, Umadevi Sajjan
Summary: Patients with chronic rhinosinusitis often have biofilms that are resistant to antibiotics. This study found that S-nitrosoglutathione (GSNO) combined with gentamicin can effectively disperse and reduce the density of biofilms in patients. GSNO treatment also improved the mucociliary clearance by increasing the ciliary beat frequency (CBF).
Article
Chemistry, Analytical
Shannon P. Wetzler-Quevedo, Mark E. Meyerhoff, Ryan C. Bailey
Summary: The influence of microfluidic parameters on droptode efficiency was examined, with channel geometry and droplet size found to significantly impact droptode performance. Increasing the size of the sample droplet can enhance sensitivity in the linear region of the droptode.
Article
Chemistry, Analytical
Xuewei Wang, Yang Zhou, Vanessa Decker, Mark Meyerhoff, Meng Sun, Yu Cui
ANALYTICAL METHODS
(2020)
Article
Nanoscience & Nanotechnology
Mark M. Jeakle, Terry C. Major, Mark E. Meyerhoff, Robert H. Bartlett
ACS APPLIED BIO MATERIALS
(2020)
