Article
Chemistry, Multidisciplinary
Cheng Ouyang, Yongguang Li, Thomas W. Rees, Xinxing Liao, Jianhua Jia, Yu Chen, Xiting Zhang, Liangnian Ji, Hui Chao
Summary: The organoplatinum(II) complex Pt1 can assemble into nanoaggregates via pi-pi stacking and complementary hydrogen bonds, exhibiting ratiometric dual emission in cells with intact membranes and disrupted membranes. The unique characteristics of Pt1 enable its use for tracking cell apoptosis, necrosis, and inflammation in zebrafish.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2021)
Article
Chemistry, Applied
Jingshuo Gao, Xu Jia, Zhenbo Guo, Shuhan Yang, Lingyu Wei, Pingzhu Zhang, Chao Wei, Guofeng Chen, Xiaoliu Li
Summary: This study successfully designed and synthesized a novel two-photon ratiometric fluorescent probe (BCD) for imaging endogenous H2S in living cells and zebrafish, showing large emission separation, excellent selectivity, and high sensitivity.
Article
Chemistry, Analytical
Javier Valverde-Pozo, Jose Manuel Paredes, Maria Eugenia Garcia-Rubino, Thomas J. Widmann, Carmen Grinan-Lison, Silvia Lobon-Moles, Juan Antonio Marchal, Jose Maria Alvarez-Pez, Eva Maria Talavera
Summary: A new fluorescent probe with large Stokes shift and high ratiometric fluorescence output was developed for the molecular imaging of highly malignant and metastatic melanomas. The probe allows for two-photon excitation, which overcomes the limitations of traditional one-photon excited probes and enables deeper tissue penetration.
Article
Spectroscopy
Wen Liu, Jinyao Li, Zicheng Wang, Yafei Tian, Guodong Ren, Xiaoyu Hou, Lixia Guo, Lihong Li, Chengwu Zhang, Zhifang Wu, Lili Yan, Sijin Li, Haipeng Diao
Summary: This study developed a ratiometric fluorescent nano platform for the detection of sulfur dioxide. The platform had mitochondria targeting ability and demonstrated advantages in sensitive detection and sensing capabilities in biological milieu.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2022)
Article
Spectroscopy
Yu-Xu Tu, Natarajan Vijay, Han-Xiang Ko, Yuan-Pin Lo, Sivan Velmathi, Shu-Pao Wu
Summary: The new fluorescent probe ANP can rapidly and efficiently detect cysteine, and it can evaluate the distribution of cysteine in living cells and tissues.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2022)
Article
Chemistry, Multidisciplinary
Javier Valverde-Pozo, Jose M. Paredes, Thomas J. Widmann, Carmen Grinan-Lison, Giada Ceccarel, Antimo Gioiello, M. Eugenia Garcia-Rubino, Juan A. . Marchal, Jose M. Alvarez-Pez, Eva M. Talavera
Summary: DPP IV, also known as CD26 lymphocyte T surface antigen, is a transmembrane glycoprotein with a wide range of functions. It is overexpressed in several cancers and can serve as a diagnostic tool. A new near-infrared fluorimetric probe has been designed to detect the activity of DPP IV in living cells and tissues, using zebrafish as a model organism.
Article
Chemistry, Multidisciplinary
Hye Rim Kim, Sourav Sarkar, Kyo Han Ahn
Summary: Fluorescent probes are essential tools for studying biological systems, but fast clearance is a concern in quantitative analysis. This study introduces a novel sensing platform that allows prolonged imaging and reliable quantitative analysis through ratiometric signaling.
CHEMISTRY-AN ASIAN JOURNAL
(2022)
Article
Chemistry, Analytical
Xiao-Chuang Chang, Ling Zhang, Bing-Jie Liu, Yan-Tao Lou, Wei-Fan Xu, Xin-Ru Li, Xue-Feng Han, Wei-Na Wu, Yuan Wang, Zhi-Hong Xu
Summary: A novel pyrene-based fluorescent probe was synthesized for the detection of hypochlorite (ClO-) with aggregation-induced ratiometric emission (AIRE) at 525 nm in aqueous solution. The probe showed a fast and sensitive fluorescent response to ClO- within 20 seconds with a detection limit of 269 nM. The sensing mechanism of the probe was investigated using various techniques, and it was successfully applied for imaging endogenous and exogenous ClO- in living cells and zebrafish.
MICROCHEMICAL JOURNAL
(2023)
Article
Chemistry, Multidisciplinary
Xin Zhang, Chunfei Wang, Gang Feng, Jianxia Jiang, Jiwen Hu, Anna du Rietz, Caroline Brommesson, Xuanjun Zhang, Yuguang Ma, Karin Roberg, Fengling Zhang, Han-Ming Shen, Kajsa Uvdal, Zhangjun Hu
Summary: The authors discussed a tunable membrane-penetrating nanoplatform to address delivery and specificity issues in nano-based bio-imaging, offering huge flexibility to achieve expected properties and functions through its tunable contents.
Article
Chemistry, Analytical
Xiu Wang, Wei Yuan, Ming Xu, Fuyou Li
Summary: The introduction of 2PIA and UCMOFs in biological imaging effectively reduces background interference, improves signal visibility, and greatly enhances spatial resolution and tissue penetration depth.
ANALYTICAL CHEMISTRY
(2021)
Article
Chemistry, Analytical
Jintao Zhang, Qian An, Wei Li, Li Chai, Wei Hu, Yanying Wang, Shengze Su, Yifan He, Chunya Li, Dong Sun
Summary: Neuroinflammation is a major destructive effect in various neurodegenerative diseases, and conventional NO sensors are not able to accurately evaluate inflammation levels. However, a lipid droplet-targeted two photon nitric oxide probe can provide a more accurate assessment of neuroinflammation levels.
SENSORS AND ACTUATORS B-CHEMICAL
(2021)
Article
Chemistry, Analytical
Wei Shu, Jin Yu, Zhuohang Li, Xiaoqian Sun, Yunpeng Wang, Yulong Wu, Jie Qin, Yu Zhang, Haibin Xiao, Xiaoling Zhang
Summary: In this study, a novel mitochondria-targeted fluorescent probe SNRF was developed for monitoring mitochondrial pH changes. SNRF showed excellent molecular charge transfer performance and could accurately track pH variations during rapamycin-induced mitophagy.
SENSORS AND ACTUATORS B-CHEMICAL
(2022)
Article
Biochemistry & Molecular Biology
Dongdong An, Linlin Shi, Tianyu Li, Hong-Yu Zhang, Yahong Chen, Xin-Qi Hao, Mao-Ping Song
Summary: Fluorescent chemosensors are used extensively in biomedical imaging, but face challenges such as small Stokes shifts, interfering signals, background noise, and self-quenching. This study presents a supramolecular cage (C-A) assembled through coordination-driven self-assembly of benzothiadiazole derivatives and Eu(OTf)(3). C-A exhibits high fluorescence with a quantum yield (QY) of 38.57%, good photoluminescence (PL) stability, and a large Stokes shift (153 nm). Additionally, the low cytotoxicity of C-A is confirmed by CCK-8 assay against U87 glioblastoma cells, making it a potential probe for U87 cell targeting bioimaging.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Chemistry, Multidisciplinary
Brandon J. Furey, Benjamin J. Stacy, Tushti Shah, Rodrigo M. Barba-Barba, Ramon Carriles, Alan Bernal, Bernardo S. Mendoza, Brian A. Korgel, Michael C. Downer
Summary: Colloidal nanocrystalline silicon quantum dots (nc-SiQDs) excited by two-photon in the near-infrared (NIR) range have potential applications in deep biological imaging. The two-photon absorption (2PA) cross section of colloidal nc-SiQDs is measured, and it is found to be smaller for smaller diameter nanocrystals. The efficiencies of nc-SiQDs for bioimaging using two-photon excited photoluminescence (2PE-PL) are compared to other quantum dots and molecular fluorophores and found to be comparable or superior at greater depths.
Article
Chemistry, Analytical
Fei-Peng Ying, Hui-Shan Lu, Xiao-Qin Yi, Yu-Qi Xu, Yuan-Yuan Lv
Summary: The innovative ratiometric fluorescent chemosensor SP, with a 3-picolyl thiourea pendant attached to a porphyrin fluorophore, selectively monitors Zn2+ in aqueous buffer and biological samples with low detection limit and high selectivity. The coordination ability between Zn2+ and nitrogen atoms allows SP to capture Zn2+ via an axial ligation.
SENSORS AND ACTUATORS B-CHEMICAL
(2021)
Article
Engineering, Biomedical
Shuai Xu, Wenjing Pan, Lanlan Chen, Sulai Liu, Tian-Bing Ren, Hong-Wen Liu, Yongchao Liu, Shuang-Yan Huan, Lin Yuan, Xiao-Bing Zhang
Summary: Developing a chemiluminescence probe with slow kinetic profile is challenging but crucial for improving analytical sensitivity. A novel strategy was reported in this study to achieve long-lasting in vivo imaging by introducing hydrogen-bond-driven self-assembled dye HPQ to Schaap's dioxetane. The self-assembled chemiluminophore HPQCL-Cl showed prolonged half-life and constant emission, enabling long-lasting imaging and image-guided clinical surgery.
Article
Chemistry, Analytical
Na Wu, Chang Lu, Youjuan Wang, Liuhui Liu, Dingyou Lu, Ying Zhou, Min He, Xiaobing Zhang, Guosheng Song
Summary: The total antioxidant capacity (TAC) is an important indicator of the body's ability to resist oxidative stress injury in diabetes. Currently, there is a lack of a simple and reliable method to detect TAC in diabetes. In this study, a novel chemiluminescent platform based on semiconducting polymer nanoparticles-manganese (SPNs-MnVII) is designed to detect the total antioxidant capacity of urine in diabetic mice.
ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Multidisciplinary
Zhiyang Yuwen, Qin Zeng, Qiaozhen Ye, Yixing Zhao, Jingxuan Zhu, Kang Chen, Hongwen Liu, Ronghua Yang
Summary: Precise quantification of trace components in whole blood via fluorescence is challenging due to strong blood autofluorescence. This study developed an activable fluorescent probe for quantification of trace analyte in whole blood by suppressing blood autofluorescence. The probe selected a redshift BODIPY quencher with high brightness and superior quenching efficiency based on inner filter effect. The detection system demonstrated accurate quantification of endogenous H2S in whole blood and showed potential for expanding to other trace analyte detection.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2023)
Review
Chemistry, Multidisciplinary
Hengxin Shen, Shiyi Liao, Zhe Li, Youjuan Wang, Shuangyan Huan, Xiao-Bing Zhang, Guosheng Song
Summary: Organic afterglow nanoparticles are optical materials that emit light long after excitation. They have been widely used in various fields such as cell tracking, biosensing, cancer diagnosis, and therapy due to their advantages of no need for real-time excitation, low background, deep tissue penetration, and high sensitivity. This review summarizes the recent progress of organic afterglow imaging, focusing on the mechanism and biological application of these materials. The potential challenges and future directions of this field are also discussed.
CHEMISTRY-A EUROPEAN JOURNAL
(2023)
Article
Chemistry, Multidisciplinary
Yongchao Liu, Lili Teng, Xiao-Feng Lou, Xiao-Bing Zhang, Guosheng Song
Summary: This study reports a novel hemicyanine-based molecule scaffold with stimuli-responsive afterglow luminescence, which can store photoenergy for delayed luminescence. Three modular activatable molecular afterglow probes (MAPs) are designed for the quantification and imaging of pH, superoxide anions, and aminopeptidase. MAPs show higher sensitivity in afterglow imaging than in fluorescence imaging due to the simultaneous control of fluorescence initiation and 1O2 generation by the responsive unit. MAPs are also applied for high-contrast afterglow imaging of drug-induced hepatotoxicity, allowing for early diagnosis.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2023)
Review
Chemistry, Analytical
Cong Hu, Shuai Xu, Zhiling Song, Haixia Li, Hongwen Liu
Summary: The nucleus is the control hub and storage center of genetic information in cells, and its morphology can be used to assess cell vitality and health. The biological molecules in the nucleus are crucial for normal cellular processes and disease development. Fluorescence imaging and fluorescent probes provide valuable tools for studying cellular processes, disease diagnosis, and treatment by targeting the nucleus.
Article
Chemistry, Analytical
Yixing Zhao, Lingyun Li, Qiaozhen Ye, Yijun Gong, Ronghua Yang, Hongwen Liu
Summary: A GSH-activatable probe MC-PSE was designed and synthesized for second near-infrared window (NIR-II) fluorescence and ratiometric photoacoustic imaging of GSH in vivo based on the self-assembly and disassembly of J-aggregation. MC-PSE tends to form stable J-aggregates in an aqueous solution. Upon the reaction with GSH, the J-aggregates of MC-PSE disassembled, resulting in a significant increase in emission peak intensity at 940 nm and PA(900)/PA(980) ratio within 15 min in vitro. By utilizing NIR-II FL and PA dual-modal imaging, MC-PSE successfully visualized GSH in tumor-bearing mice and distinguished normal and tumor areas.
ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Multidisciplinary
Yuan Ma, Jinhui Shang, Liuhui Liu, Menghuan Li, Xinyu Xu, Hui Cao, Li Xu, Wei Sun, Guosheng Song, Xiao-Bing Zhang
Summary: Atherosclerotic plaque rupture, caused by the decomposition of fiber caps induced by cysteine cathepsin, is a significant cause of acute cardiovascular events. However, the accurate measurement of cathepsin B (CTB) activity in plaques is challenging due to the limitations of current fluorescent probes. To address this, a lipid-unlocked CTB responsive probe (L-CRP) was developed, which uses lipids and CTB as two keys to unlock photoacoustic signals for measuring CTB activity in lipophilic environments. L-CRP demonstrates reliable imaging of specific CTB activities in foam cells and atherosclerotic plaques, and exhibits deeper tissue penetration ability compared to current CTB probes. In atherosclerotic mice, L-CRP dynamically reports intraplaque CTB levels and enables risk stratification based on plaque vulnerability characteristics. L-CRP also successfully identifies atherosclerotic plaques in excised human artery tissues, showing promise for auxiliary diagnosis in clinical applications.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2023)
Article
Chemistry, Multidisciplinary
Lingling Lei, Fengrui Yang, Xin Meng, Li Xu, Peng Liang, Yuan Ma, Zhe Dong, Youjuan Wang, Xiao-Bing Zhang, Guosheng Song
Summary: This study presents a unique afterglow molecular scaffold based on hemicyanine and rhodamine dyes, which shows promise for low-background optical imaging. Compared to previous designs, this scaffold exhibits responsive OFF-ON afterglow signals and offers advantages such as a larger dynamic range and higher sensitivity. The integration of multiple functionalities simplifies probe design and enhances reliability. Additionally, the researchers have developed various probes for different molecular targets, expanding the applications of this afterglow molecular scaffold.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2023)
Review
Chemistry, Multidisciplinary
Baode Chen, Liuhui Liu, Renye Yue, Zhe Dong, Chang Lu, Cheng Zhang, Guoqiang Guan, Huiyi Liu, Qingpeng Zhang, Guosheng Song
Summary: With the advancement in MRI technology, traditional contrast agents struggle to meet accuracy imaging demands, but novel dual-mode contrast agents with T1-T2 ability provide better imaging applications and improved accuracy. Responsive MRI probes, unlike traditional agents, change signals in response to stimuli, leading to highly selective and excellent contrast effects. This review focuses on responsive and switchable MRI probe types, highlighting their mechanism, application in tumor imaging, and future development trends and challenges.
Review
Nanoscience & Nanotechnology
Qinpeng Zhang, Rui Yin, Guoqiang Guan, Huiyi Liu, Guosheng Song
Summary: This review provides an overview of recent advancements in the development of renal-clearable magnetic nanoparticles (MNPs) for disease imaging and treatment. Factors influencing renal clearance, types of renal-clearable MNPs, synthesis methods, and biomedical applications are discussed.
WILEY INTERDISCIPLINARY REVIEWS-NANOMEDICINE AND NANOBIOTECHNOLOGY
(2023)
Article
Chemistry, Multidisciplinary
Chang Lu, Zhe Li, Na Wu, Dingyou Lu, Xiao-Bing Zhang, Guosheng Song
Summary: Acidity-stimulated platforms have the potential to improve cancer imaging and therapy within the tumor microenvironment. This study developed a novel companion diagnostic system, core-satellite semiconducting polymer nanoparticles @ cobalt hydroxide oxide (SPNs@CoOOH), tailored to tumor microenvironments. The pH-responsive range of SPNs@CoOOH can be regulated to achieve efficient and precise tumor treatment, minimizing non-specific activation in normal tissue and off-targeted toxicity risks. The chemiluminescence from SPNs@CoOOH correlates well with the generation of (1)O(2) under different conditions, showing good relevance with cancer inhibition rates in vivo.
Review
Materials Science, Biomaterials
Zhe Dong, Peng Liang, Youjuan Wang, Guoqiang Guan, Lili Teng, Renye Yue, Chang Lu, Shuangyan Huan, Xia Yin, Guosheng Song
Summary: Nanozymes are nanoscale materials with enzyme-like properties that have been improved to overcome the limitations of natural enzymes and expand the applications of artificial enzymes. The research and exploration of nanozymes have attracted considerable attention in the chemical and biological fields, particularly in biomedicine and tumor therapy. Many nanozymes have been developed with activities similar to natural enzymes, such as peroxidase, catalase, superoxide dismutase, and glucose oxidase. However, the catalytic activity of nanozymes varies significantly between in vitro and in vivo environments, and the complex tumor environment can affect their activity. To address these challenges, researchers have employed various imaging methods to monitor the catalytic activity of nanozymes, including optical imaging, photoacoustic imaging, and magnetic resonance imaging. This review summarizes recent developments in tumor treatment using nanozymes and the current imaging tools for monitoring their catalytic activity.
JOURNAL OF MATERIALS CHEMISTRY B
(2023)
Article
Chemistry, Analytical
Na Wu, Chang Lu, Youjuan Wang, Liuhui Liu, Dingyou Lu, Ying Zhou, Min He, Xiaobing Zhang, Guosheng Song
Summary: A novel chemiluminescent platform based on semiconducting polymer nanoparticles-manganese (SPNs-MnVII) was designed to detect the total antioxidant capacity of urine in diabetic mice. The chemiluminescent tool of SPNs (PFODBT)-MnVII is convenient, efficient, and sensitive, and shows promise for monitoring antioxidant therapy of diabetes.
ANALYTICAL CHEMISTRY
(2023)
Review
Chemistry, Multidisciplinary
Zhiming Deng, Dingyou Lu, Sulai Liu, Xianzheng Tan, Guosheng Song
Summary: Disorder of metal ion levels can significantly impact the ecological environment and human health. Traditional detection methods have limitations in monitoring the physiological distribution of metal ions with high sensitivity. Magnetic resonance imaging (MRI) has been widely used to design and develop MRI nanoprobes for metal ion detection. This review highlights the research progress, limitations, and challenges in the development of MRI-based metal ion detection probes.
MATERIALS CHEMISTRY FRONTIERS
(2023)