Article
Multidisciplinary Sciences
George Alzeeb, Danielle Arzur, Valerie Trichet, Matthieu Talagas, Laurent Corcos, Catherine Le Jossic-Corcos
Summary: Gastric cancer is the third leading cause of cancer-related mortality worldwide, often diagnosed at advanced stages. Utilizing the cholesterol-lowering drug lovastatin as an adjuvant to chemotherapy has shown significant efficacy in treating gastric cancer. In a 3D model, the combination of lovastatin and docetaxel effectively eliminates growing gastric cancer cells.
SCIENTIFIC REPORTS
(2022)
Review
Pharmacology & Pharmacy
Sigrid A. Langhans
Summary: There has been a rapid increase in 3D cell culture technologies in recent years aimed at closely mimicking in vivo physiology. Each 3D cell culture system has its own strengths and weaknesses when it comes to in vivo tumor growth and the tumor microenvironment, which requires careful consideration when choosing a system for drug discovery based on factors like drug target and tumor origin.
EXPERT OPINION ON DRUG DISCOVERY
(2021)
Article
Chemistry, Analytical
Huijung Kim, Yoojoong Han, Intan Rosalina Suhito, Yoon Choi, Minkyeong Kwon, Hyungbin Son, Hyung-Ryong Kim, Tae-Hyung Kim
Summary: This study utilized a 3D Raman mapping-based analytical method to investigate the 3D cellular differentiation of human dental pulp stem cell spheroids under different culture conditions. It was found that the diffusion of the medium played a crucial role in determining the variations in stem cell spheroid differentiation.
ANALYTICAL CHEMISTRY
(2021)
Article
Chemistry, Multidisciplinary
Shuneng Zhou, Junfeng Yang, Rui Li, Yiyu Chen, Chengpan Li, Chao Chen, Yuan Tao, Shengying Fan, Dong Wu, Li Wen, Bensheng Qiu, Weiping Ding
Summary: In this study, a flexible 3D hanging drop construction platform was developed for easy construction of hanging drops of varying sizes, types, and patterns. This platform enables live imaging and has great potential for personalized medicine, cancer and drug discovery, tissue engineering, and stem cell research.
Article
Biology
Arianna Langella, Sergio Domenico Gadau, Elisa Serra, Daniela Bebbere, Sergio Ledda
Summary: In this study, two 3D culture systems, liquid marbles (LMs) and hanging drops (HD), were used to generate spheroids from C6 rat glioma cells. The spheroids formed more rapidly in the LM system and showed higher levels of tubulin post-translational modifications (PTMs) compared to the HD system. These findings suggest that the LM 3D system enhances the plasticity state of glioma cells and could be used to evaluate new anticancer therapies.
Review
Biochemistry & Molecular Biology
Elisabetta Colombo, Maria Grazia Cattaneo
Summary: 2D cell cultures have been the standard in various applications, but they cannot replicate the complex and dynamic interactions of the tumor microenvironment (TME). Multicellular 3D models are important for studying the spatial dimension, cellular heterogeneity, and molecular networks of the TME.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Chemistry, Multidisciplinary
Sujeong Ahn, Dohyun Kim, Kanghee Cho, Won-Gun Koh
Summary: Three-dimensional cell culture systems, especially spheroids, provide a more accurate representation of the complex human body structure compared to monolayer cell cultures. However, conventional spheroid fabrication methods have limitations for mass production and size uniformity. Microfabrication methods, such as micropatterning, 3D bioprinting, and microfluidics, have been proposed as alternatives to overcome these limitations. These methods allow for the mass production, precise size control, and localization of spheroids, enhancing their effectiveness in various biomedical applications such as regenerative medicine, tumor microenvironment research, drug screening, and organoid fabrication.
KOREAN JOURNAL OF CHEMICAL ENGINEERING
(2023)
Review
Engineering, Biomedical
Yanshen Yang, Yuanbo Jia, Qingzhen Yang, Feng Xu
Summary: Three-dimensional bioprinting is a promising approach to construct functional biomimetic tissues. Bio-inks, which are essential for cell microenvironment construction, are influenced by mechanical properties. With the advancement of functional biomaterials, engineered bio-inks have allowed the creation of cell mechanical microenvironments in vitro.
INTERNATIONAL JOURNAL OF BIOPRINTING
(2023)
Review
Engineering, Biomedical
Pei Zhuang, Yi-Hua Chiang, Maria Serafim Fernanda, Mei He
Summary: Although cancer remains a leading cause of mortality worldwide, efforts in anticancer therapeutics are hindered by the lack of robust prediction models. Multicellular tumor spheroids, a popular three-dimensional culture model for avascular tumors, have limitations in controlling cellular and structural organization. 3D bioprinting, with its spatial control, scalability, and reproducibility, holds promise for generating more faithful tumor models and advancing our understanding of tumor progression.
INTERNATIONAL JOURNAL OF BIOPRINTING
(2021)
Article
Chemistry, Multidisciplinary
Yanjia Yu, Mohan Dai, Wei Xu, Shuangshuang Chen, Qinghua Lu
Summary: A novel upward culture method on a porous silica superamphiphobic biointerface (SABI) is developed to efficiently establish large-volume 3D cell spheroids within a short time and allow long-term culture. This method overcomes size limitation and media refreshing issues in traditional methods.
ADVANCED MATERIALS INTERFACES
(2021)
Article
Engineering, Mechanical
Pudi Wang, Qing Zhang, Xu Fang, Feng Lin, Jianyong Huang
Summary: This passage discusses the mechanical mechanisms of cell volume regulation in a 3D microenvironment, revealing a competitive relationship between intracellular cortices and 3D ECM, as well as the joint regulation of cell growth and volume adaption by factors such as initial cell size and ECM stiffness. It also highlights the critical role of prestresses on cell-ECM interfaces in constraining 3D cell growth, and the geometric size-dependent sensitivity of cell volume variation to external stimuli.
EXTREME MECHANICS LETTERS
(2021)
Article
Nanoscience & Nanotechnology
Nassim Rousset, Ruben Lopez Sandoval, Mario Matteo Modena, Andreas Hierlemann, Patrick M. Misun
Summary: As 3D in vitro tissue models become more prevalent, analyzing the gradients and heterogeneity of nutrient and metabolite concentrations becomes more challenging. In this study, the researchers used electrochemical biosensors to measure glucose concentrations around tissue models, allowing them to study size-dependent metabolism data.
MICROSYSTEMS & NANOENGINEERING
(2022)
Article
Multidisciplinary Sciences
Wei Hou, Zilin Zhang, Jing Zhang, Shihui Xu, Menglan Li, Xiaoran Li, Zaozao Chen, Cailian Wang
Summary: In addition to monolayer-cultured cells, three-dimensional tumor spheroids have been developed as a potentially powerful tool for evaluating anticancer drugs. However, conventional culture methods lack the ability to manipulate tumor spheroids in a homogeneous manner at the three-dimensional level. This paper presents a convenient and effective method for constructing average-sized tumor spheroids. Furthermore, it describes an image-based analysis method using artificial intelligence-based software to scan the entire plate and obtain data on three-dimensional spheroids. Several parameters were studied. By using a standardized method of tumor spheroid construction and a high-throughput imaging and analysis system, the effectiveness and accuracy of drug tests performed on three-dimensional spheroids can be significantly improved.
JOVE-JOURNAL OF VISUALIZED EXPERIMENTS
(2023)
Article
Cell & Tissue Engineering
Takayoshi Oba, Satoshi Okamoto, Yasuharu Ueno, Megumi Matsuo, Tomomi Tadokoro, Shinji Kobayashi, Kazunori Yasumura, Shintaro Kagimoto, Yutaka Inaba, Hideki Taniguchi
Summary: In this study, a novel in vitro scaffold-free method for elastic cartilage reconstruction using human auricular perichondrial chondroprogenitor cells was developed. Inducing chondrogenesis with microscopic spheroids increased the chondrogenic potential. Craniofacial transplantation in immunodeficient mice showed that the reconstructed tissue was morphologically stable.
JOURNAL OF TISSUE ENGINEERING
(2022)
Article
Biotechnology & Applied Microbiology
Yoon Jeong, Ashley Tin, Joseph Irudayaraj
Summary: The study introduces a straightforward and user-friendly method for 3D tumor culture using standard 96-well plates, which can generate large tumor spheroids and has wide applications.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)