期刊
ACS CHEMICAL NEUROSCIENCE
卷 10, 期 8, 页码 3555-3564出版社
AMER CHEMICAL SOC
DOI: 10.1021/acschemneuro.9b00177
关键词
Beta-amyloid; BIM; Bcl-3 homology 3; mitochondria; apoptosis; amyloid/membrane interaction
Extensive neuronal cell death is among the pathological hallmarks of Alzheimer's disease. While neuron death is coincident with formation of plaques comprising the beta-amyloid (A beta) peptide, a direct causative link between A beta (or other Alzheimer's-associated proteins) and cell toxicity is yet to be found. Here we show that BIM-BH3, the primary proapoptotic domain of BIM, a key protein in varied apoptotic cascades of which elevated levels have been found in brain cells of patients afflicted with Alzheimer's disease, interacts with the 42-residue amyloid isoform A beta 42. Remarkably, BIM-BH3 modulated the structure, fibrillation pathway, aggregate morphology, and membrane interactions of A beta 42. In particular, BIM-BH3 inhibited A beta 42 fibril-formation, while it simultaneously enhanced protofibril assembly. Furthermore, we discovered that BIM-BH3/A beta 42 interactions induced cell death in a human neuroblastoma cell model. Overall, our data provide a crucial mechanistic link accounting for neuronal cell death in Alzheimer's disease patients and the participation of both BIM and A beta 42 in the neurotoxicity process.
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