Article
Chemistry, Physical
Lucas A. de Freitas, Marylane de Sousa, Laiza B. Ribeiro, Italo W. L. de Franca, Luciana R. B. Goncalves
Summary: This study proposes cross-linked magnetic cross-linked enzyme aggregates (mCLEAs) for the preparation of heterogeneous biocatalysts of beta-galactosidase, and investigates the concentration of cross-linking agents glutaraldehyde and dextran-aldehyde. The use of glutaraldehyde results in increased recovered activity, while the addition of a competitive inhibitor or polymer coating in the preparation process does not enhance the activity. Biocatalysts prepared with dextran-aldehyde exhibit higher recovered activity and thermal stability, making them suitable for industrial use.
Article
Food Science & Technology
Ruiting Zhao, Kang Xu, Mengyuan Yan, Jiahui Peng, Haoran Liu, Siling Huang, Susu Zhang, Zhenshang Xu, Xueping Guo, Ting Wang
Summary: A novel l-arabinose isomerase gene was identified in Lactobacillus rhamnosus, which showed good thermal stability and pH stability. It has great potential for industrial application in sweetened milk and yogurt preparation.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2023)
Article
Food Science & Technology
Zhixin Wang, Jingjie Qi, Julie M. Goddard
Summary: The addition of trehalose, glucose, and sucrose was found to improve the stability of lactase under certain environmental conditions, prolonging its activity retention time. This enhanced stability opens up new possibilities for the application of lactase in bioprocessing.
INTERNATIONAL DAIRY JOURNAL
(2021)
Article
Biochemistry & Molecular Biology
Azra Shafi, Faizan Ahmed, Qayyum Husain
Summary: β-Galactosidase was immobilized on modified nanosilver reduced graphene oxide (Ag@rGO) nanocomposite prepared by in vitro synthesis using same enzyme. The immobilized enzyme showed enhanced activity with an effectiveness factor (eta value) of 0.968, and retained 85% activity after repeated use. The enzyme displayed high activity even in the presence of inhibitory sugars, and effectively hydrolyzed lactose from whey. Additionally, the cytotoxicity and genotoxicity of Ag@rGO nanocomposite was assessed on human blood lymphocytes.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Article
Food Science & Technology
Susu Zhang, Zhenshang Xu, Ming Ma, Guoyan Zhao, Runlei Chang, Hongli Si, Meixue Dai
Summary: A new strain of Lactococcus lactis capable of producing D-tagatose was isolated, and an efficient biotransformation system was established to convert lactose to D-tagatose.
Article
Biotechnology & Applied Microbiology
Camila Regina Hackenhaar, Luiza Strapasson Spolidoro, Eli Emanuel Esparza Flores, Manuela Poletto Klein, Plinho Francisco Hertz
Summary: The study found that the immobilized beta-galactosidase exhibited good stability at high temperatures and successfully synthesized galactooligosaccharides (GOS), potentially providing valuable prebiotic sugar from by-products of milk processing.
BIOCATALYSIS AND AGRICULTURAL BIOTECHNOLOGY
(2021)
Article
Agricultural Engineering
Guoyan Zhang, Hossain M. Zabed, Yingfeng An, Junhua Yun, Jiaqi Huang, Yufei Zhang, Xiaolan Li, Jiangfei Wang, Yuvaraj Ravikumar, Xianghui Qi
Summary: In this study, a lactose-rich dairy byproduct, cheese whey powder, was efficiently converted into three low caloric sweeteners using microbial fermentation technologies. The three rare sugars produced showed potential as commercial products, demonstrating the value of utilizing dairy industry waste.
BIORESOURCE TECHNOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Zhi-Peng Wang, Xin-Yue Zhang, Yan Ma, Jing-Run Ye, Jing Jiang, Hai-Ying Wang, Wei Chen
Summary: This study demonstrates the efficient accumulation of lipid by Aureobasidium namibiae A12 from agro-industrial wastes rich in galactose-based carbohydrates, with key enzymes identified. Moreover, the significance of sucrase in the direct hydrolysis of galactose-based carbohydrates has been revealed for the first time.
BIOTECHNOLOGY FOR BIOFUELS
(2021)
Review
Food Science & Technology
Maria Micaela Ureta, Goncalo Nuno Martins, Onofre Figueira, Pedro Filipe Pires, Paula Cristina Castilho, Andrea Gomez-Zavaglia
Summary: This review explores principal immobilization strategies aimed at achieving optimal industrial processes, focusing specifically on beta-galactosidases and fructosyltransferases. The main methods for immobilizing these enzymes include adsorption, entrapment, covalent coupling, and cross-linking or aggregation. It is important for the support material to be cost-effective, ensure enzyme reusability and easy recovery, and enhance stability.
CRITICAL REVIEWS IN FOOD SCIENCE AND NUTRITION
(2021)
Article
Food Science & Technology
Wei Liu, Zhilin Zhang, Ying Li, Liying Zhu, Ling Jiang
Summary: D-Tagatose, a emerging sweetener, can be synthesized from lactose and whey powder with high yield and purity through enzymatic reactions.
FOOD RESEARCH INTERNATIONAL
(2023)
Article
Chemistry, Multidisciplinary
Duana Ceciliane Hanauer, Alana Gabrieli de Souza, Mariana Aguiar Cargnin, Bruna Carla Gasparin, Derval dos Santos Rosa, Alexandre Tadeu Paulino
Summary: In this study, pectin-based biohydrogels were synthesized with or without eucalyptus sawdust, and used for immobilizing beta-D-galactosidase for lactose hydrolysis reactions. The addition of eucalyptus sawdust increased the thermal and mechanical resistance of the biomaterial, preserving enzyme stability. The immobilized enzyme in pectin-based biohydrogels showed potential for producing lactose-free or low-dosage lactose foods.
JOURNAL OF INDUSTRIAL AND ENGINEERING CHEMISTRY
(2021)
Article
Biochemistry & Molecular Biology
Shunsuke Tomino, Akihide Yoshihara, George W. J. Fleet, Ken Izumori
Summary: In this study, d-aldotetroses were successfully produced using 3 isomerases through chemical synthesis. First, l-erythrulose was converted to d-erythrulose using d-tagatose 3-epimerase. The d, l-erythrulose mixture was then isomerized to d-threose with d-arabinose isomerase, and d-erythrose was produced using l-rhamnose isomerase.
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
(2023)
Article
Chemistry, Applied
Yao Meng, Xiao Zhao, Yunqing Jiang, Qingfeng Ban, Xibo Wang
Summary: The Maillard reaction was used to improve the functionality of proteins by covalent interaction with monosaccharides. This study prepared WPI/DT conjugates using dry heating treatment and investigated the effects of different Maillard reaction conditions on the structure and properties of the conjugates. The Maillard reaction significantly changed the gel properties and thermal stability of the WPI/DT conjugates. The study provides a theoretical basis for utilizing DT as a food ingredient to enhance the functional properties of WPI.
Article
Chemistry, Physical
Shushil Kumar Rai, Varun Kumar, Sudesh Kumar Yadav
Summary: This study successfully synthesized a high value rare sugar d-tagatose using recyclable magnetic catalysts and developed two novel magnetic cross-linked enzyme aggregates for efficient transformation of d-galactose and lactose. The immobilized catalysts showed high catalytic activity and reusability potential, indicating promising catalytic potential for the synthesis of d-tagatose.
CATALYSIS SCIENCE & TECHNOLOGY
(2021)
Article
Chemistry, Physical
Sami A. Al-Harbi, Yaaser Q. Almulaiky
Summary: The immobilization of beta-galactosidase on Cu-trimesic acid-based metal organic frameworks (Cu-TMAs) was studied. The immobilized enzyme showed good retention of activity, storage stability, and improved lactose hydrolysis activity. This study demonstrated the potential of Cu-TMAs as supports for immobilizing beta-galactosidase and suggested practical applications in various industrial processes.
JOURNAL OF MOLECULAR LIQUIDS
(2023)
Article
Chemistry, Physical
Haojie Li, Bingke Yang, Zhen Yao, Xuetao Wang, Kaiming Shen, Mengjie Liu
Summary: This study systematically investigates the influence of metal and nonmetal element doping on the photochemical properties of g-C3N4 for efficient catalytic AB hydrogen production. It provides a design method for high performance bifunctional catalysts of photocatalysis and metal catalysis. The results show that both non-metals (B, P) and metals (Ru, Ni) have efficient regulatory effects on the band structure of g-C3N4, resulting in a reduced band gap and improved hydrogen production. The study offers a theoretical method for the coupling of metal catalysis/photocatalysis ammonia borane to produce hydrogen.
MOLECULAR CATALYSIS
(2024)
Article
Chemistry, Physical
Shrouq Mujahed, Davide Gandolfo, Luigi Vaccaro, Evgueni Kirillov, Dmitri Gelman
Summary: In this study, a high-valent Ru(IV) bifunctional catalyst was successfully applied for the hydrosilylation of various functional groups. The high-valent hydride complexes showed high chemoselectivity and affinity towards reducing polar bonds. The scope, limitations, and plausible mechanism of the reaction were described.
MOLECULAR CATALYSIS
(2024)
Article
Chemistry, Physical
Yongzhen Peng, Kongchen Xia, Qi Wu
Summary: In this study, we report an engineered cyclohexanone monooxygenase that can be used for the asymmetric synthesis of chiral alpha-deuterated carbonyl compounds via enantioselective reductive dehalogenation. The engineered enzyme exhibits good chemoselectivity, stereoselectivity, and d-incorporation, making it a promising method for the synthesis of deuterated drugs.
MOLECULAR CATALYSIS
(2024)
Article
Chemistry, Physical
Qi Yang, Ruixuan Xu, Hongqi Nie, Qilong Yan, Jun Liu, Jiuyu Chen, Yunlan Sun
Summary: The adsorption and decomposition processes of ammonium perchlorate (AP) on pure-Al and Al2O3/Al surfaces were investigated using density functional theory. The results showed that the pure-Al surface is more conducive to the decomposition of AP and the activation of NH3, while the Al2O3/Al surface promotes the disengagement of H and NH3 decomposition.
MOLECULAR CATALYSIS
(2024)
Article
Chemistry, Physical
Yudong Hu, Guochao Xu, Ye Ni
Summary: This study identified a novel phenylalanine dehydrogenase (QtPDH) with high catalytic efficiency and thermal stability, making it a promising biocatalyst for industrial production of bulky aromatic primary amines. QtPDH exhibited a broader substrate specificity and significantly longer half-life compared to BbPDH.
MOLECULAR CATALYSIS
(2024)
Article
Chemistry, Physical
Weiling Zhao, Zhiling Huang, Hui Shen, Xianglong Li, Shaofen Zhao, Bo Xie, Shengjie Xia
Summary: This study investigated the effects of metal doping and crystal plane selection on the CO2 adsorption properties of MgO using density functional theory (DFT) methods. The results showed that the appropriate crystal plane and metal doping can improve the adsorption properties of MgO on CO2. The influence of different crystal planes and metal dopants on CO2 adsorption properties varied significantly. The research provides some references for experimental studies on CO2 adsorption by MgO by combining the dual modification of crystal plane and doped metal.
MOLECULAR CATALYSIS
(2024)
Article
Chemistry, Physical
Jie Zhang, Jinwei Chen, Zongbo Shi, Junyu Zhao, Runsheng Zhuo, Ruilin Wang
Summary: In this study, a double-layered silicalite-1 support with high specific surface area was synthesized, and a magnesium modification strategy was adopted to improve the catalytic activity and stability of the zinc-based catalyst. The modified catalyst showed enhanced propylene selectivity and anti-coking property.
MOLECULAR CATALYSIS
(2024)
Article
Chemistry, Physical
Pei-Sen Gao, Chang-Wang Pan, Cheng Liu, Wen -Tong Chen
Summary: Asymmetric electrocatalysis offers a unique approach to obtain enantioenriched molecules that are difficult to obtain through conventional methods. This study developed a novel bifunctional electrocatalyst, enabling the oxidative kinetic resolution of chiral 1,4-diols and gamma-lactones. The work demonstrates the potential of bifunctional electrocatalysis for asymmetric synthetic methods and its importance in the development of novel electrocatalytic methods.
MOLECULAR CATALYSIS
(2024)
Article
Chemistry, Physical
Qinzhe Lin, Xuanjin Lv, Xianming Zeng, Mengning Zhong, Qiyun Wu, Huilin Ren, Shenpeng Xu, Wentian Chen, Wenting Du, Jun Li
Summary: The catalytic efficiency of engineered IRED M5 was found to be relatively low when tasked with a bulky amine substrate. Rational design led to the mutants M203V and F260A, with F260A exhibiting a substantial improvement in conversion and stereoselectivity. The study revealed the potential molecular mechanisms underlying the effect of F260A and M203V on catalytic performance.
MOLECULAR CATALYSIS
(2024)
Article
Chemistry, Physical
Zhishuai Wang, Fengyun Su, Hailong Cao, Mengzhen Tian, Xiang Li, Haiquan Xie, Xiaoli Jin, Zhengdao Li, Xin Ying Kong
Summary: In this study, the efficiency of photocatalytic CO2 reduction was significantly enhanced by incorporating nickel oxide onto niobium pentoxide. The resulting catalyst showed remarkable methane and carbon monoxide production improvements.
MOLECULAR CATALYSIS
(2024)
Article
Chemistry, Physical
A. Yu. Sidorenko, Yu. M. Kurban, T. V. Khalimonyuk, I. V. Il'ina, N. S. Li-Zhulanov, O. S. Patrusheva, V. V. Goltsova, M. P. Bei, Zh. V. Ihnatovich, J. Warna, K. P. Volcho, N. F. Salakhutdinov, D. Yu. Murzin, V. E. Agabekov
Summary: This study provides a comprehensive investigation into the catalytic condensation of renewable 3-carene with formaldehyde for the one-step preparation of terpenoid trans-4-hydroxymethyl-2-carene. Various acids and alumino-silicates were found to catalyze the reaction, but the selectivity to the desired product was limited. Phosphoric acid showed the highest selectivity, and an excess of formaldehyde or catalyst loading significantly increased the yield of the target product. Water presence also led to increased selectivity. Additionally, a detailed mechanism for the 3-carene condensation with formaldehyde was proposed and confirmed through kinetic modeling.
MOLECULAR CATALYSIS
(2024)
Article
Chemistry, Physical
Meng-Yu Rong, Jing Nie, Shen Li, Jun-An Ma
Summary: We synthesized a new class of chiral quaternary ammonium salts and used them as catalysts in phase-transfer catalytic asymmetric alkylation. By employing these catalysts, we obtained a series of chiral fluorinated aromatic alpha-amino acid derivatives with high yields and enantioselectivities.
MOLECULAR CATALYSIS
(2024)
Article
Chemistry, Physical
Zhaozhou Wei, Guangtao Wei, Huixian Che, Deyuan Xiong, Linye Zhang, Ruihua Xue, Yalin Tang, Xuanli Lu
Summary: This study demonstrates that small-sized graphite crystallite and highly defective carbon-based catalysts can increase the -SO3H density of the catalysts and modulate their surface electronic properties, leading to improved efficiency in the alpha-pinene hydration reaction. The reduction of graphite crystallite size is considered a critical step in enhancing the selectivity of alpha-terpineol.
MOLECULAR CATALYSIS
(2024)
Article
Chemistry, Physical
Kempanna S. Kanakikodi, Nagendra Kulal, K. S. Subramanya, M. S. Puneethkumar, Bhavana B. Kulkarni, Ganapati Shanbhag, Sanjeev P. Maradur
Summary: An effective and highly selective protocol for synthesizing asymmetric organic carbonates using dimethyl carbonate (DMC) as a reactant and solvent has been developed. The performance of CeO2 nanostructures with different morphologies in the carbonate interchange reaction (CIR) of alcohols was investigated, and the CeO2 nano-catalyst with rod morphology exhibited the highest oxygen vacancy and remarkable enhancement in conversion. The CeO2 characterization data revealed that the exposed active sites, defect density, coordination state of surface atoms, and reducibility of the catalytic materials are the contributing factors to its high catalytic activity. CeO2 can be easily recovered and reused for multiple cycles.
MOLECULAR CATALYSIS
(2024)
Article
Chemistry, Physical
Yanzhao Gao, Xianglei Meng, Shiqi Huang, Hui Wu, Liantao Jiang, Yu Zhou, Yuting Song, Yanyan Diao
Summary: Gamma alumina modified with alkaline earth metal shows improved catalytic performance for hydrogenation reactions. Pd catalysts supported by Al2O3 and Mg-modified Al2O3 were synthesized and their structure, composition, and surface acidity were investigated. The results showed that Pd/MgO-Al2O3-2 catalyst exhibited the best catalytic performance due to its metallic state palladium and weak acid sites.
MOLECULAR CATALYSIS
(2024)