Article
Pharmacology & Pharmacy
Hsiang-Hao Hsu, Aline Yen Ling Wang, Charles Yuen Yung Loh, Ashwin Alke Pai, Huang-Kai Kao
Summary: This study found that diabetic ASC-Exo enhance cutaneous wound healing by stimulating dermal cell proliferation, keratinocyte proliferation, and angiogenesis. The diabetic ASC-Exo also stimulate resident monocytes/macrophages to secrete more TGF-beta 1 and activate the TGF-beta/Smad3 signaling pathway.
Review
Cell Biology
Yang An, Shuyan Lin, Xiaojie Tan, Shiou Zhu, Fangfei Nie, Yonghuan Zhen, Luosha Gu, Chunlei Zhang, Baicheng Wang, Wei Wei, Dong Li, Junhao Wu
Summary: ADSCs-EXOs modulate immune responses and inflammation, promote angiogenesis, accelerate proliferation and re-epithelization of skin cells, and regulate collagen remodelling to inhibit scar hyperplasia in wounds. Compared with ADSCs therapeutics, ADSCs-EXOs have high stability, easy storage, immune system non-rejection, homing effect, and easily controlled dosage. In patients with diabetes mellitus, ADSCs-EXOs can improve fat grafting and promote wound healing.
CELL PROLIFERATION
(2021)
Article
Biochemistry & Molecular Biology
Armin Zakeri, Sanaz Khaseb, Mahshid Akhavan Rahnama, Akram Hajaliaskari, Mina Soufi Zomorrod
Summary: Skin wound healing involves molecular and cellular procedures in four phases: hemostasis, inflammation, proliferation, and tissue remodeling. Mesenchymal stem cells (MSCs) accelerate wound healing through their secretion of exosomes, which mimic the effects of parent MSCs. Exosomes are small vesicles that carry various biomolecules and can modulate the activity of recipient cells. MSC-derived exosomes reduce inflammation, promote proliferation, inhibit apoptosis, and enhance angiogenesis in skin wound healing. Exosomes are emerging as novel mediators for cell-cell communication and potential cell-free therapies.
Article
Anatomy & Morphology
Huldani Huldani, Iuliia A. Kozlitina, Mesfer Alshahrani, Hamid Mahmood Abdullah Daabo, Sami G. Almalki, Khulood H. Oudaha, Ahmed Hussien Alawadi, Ali Alsalamy, S. K. Joshi, Yasser Fakri Mustafa
Summary: This study investigated the therapeutic benefits of adipose stem cell-derived exosomes loaded on hyaluronic acid (HA) for diabetic wound healing in rats. The results showed that treatment with HA and exosomes promoted wound closure, increased the volumes of new epidermis and dermis, enhanced fibroblast density, blood vessel length density, collagen density, and improved tensiometrical parameters of the healed wounds. Additionally, the expression of TGF-beta and VEGF genes was upregulated, while TNF-alpha and IL-1 beta expression and neutrophil density were decreased. The combination of HA and exosomes had a greater effect on diabetic wound healing.
Review
Cell & Tissue Engineering
Chuchao Zhou, Boyu Zhang, Yanqing Yang, Qiong Jiang, Tianyu Li, Jun Gong, Hongbo Tang, Qi Zhang
Summary: Wound healing is a complex process involving hemostasis, inflammation, proliferation, and tissue remodeling. Mesenchymal stem cell-derived exosomes (MSC-exos) have lower immunogenicity and high biological activity, and play a role in regulating the behavior of various skin cells. This study focuses on exploring the specific roles and mechanisms of different MSC-exos in wound healing, as well as their limitations and potential applications. Understanding the biological properties of MSC-exos is crucial for developing cell-free therapies for wound healing and cutaneous regeneration.
STEM CELL RESEARCH & THERAPY
(2023)
Article
Biotechnology & Applied Microbiology
Yiqiang Hu, Ranyang Tao, Lang Chen, Yuan Xiong, Hang Xue, Liangcong Hu, Chenchen Yan, Xudong Xie, Ze Lin, Adriana C. Panayi, Bobin Mi, Guohui Liu
Summary: This study investigated the effect of exosomes derived from pioglitazone-pretreated MSCs on diabetic wound healing. The results showed that pioglitazone-pretreated exosomes enhanced the angiogenic function of HUVECs and accelerated diabetic wound healing. The findings suggest a promising novel cell-free therapy for treating diabetic wound healing.
JOURNAL OF NANOBIOTECHNOLOGY
(2021)
Article
Nanoscience & Nanotechnology
Jing Li, Hong Gao, Yue Xiong, Ling Wang, Haojie Zhang, Fumei He, Jingxin Zhao, Shuna Liu, Liqian Gao, Ying Guo, Wenbin Deng
Summary: This study found that exosomes derived from neural stem cells may enhance wound healing through neurotrophic factors and immunomodulation.
INTERNATIONAL JOURNAL OF NANOMEDICINE
(2022)
Review
Biochemistry & Molecular Biology
Enzo Manchon, Nell Hirt, Jean-David Bouaziz, Nabila Jabrane-Ferrat, Reem Al-Daccak
Summary: Endosome-derived small extracellular vesicles (EVs), also known as exosomes, play a critical role in intercellular communication and have therapeutic potential in regenerative medicine. Stem cell-derived EVs are effective in repair and regeneration, with the human placenta offering a promising allogeneic stem cell source. Advancements in cell-free therapeutics provide new hope and perspectives for safe and effective treatment.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Cell & Tissue Engineering
Yang Zhou, Xing-Liao Zhang, Shou-Tao Lu, Ning-Yan Zhang, Hai-Jun Zhang, Jing Zhang, Jun Zhang
Summary: This study explores the potential clinical application of hADSCs-Exos encapsulated in PF-127 hydrogel for wound healing. The results showed that PF-127/hADSCs-Exos complexes enhanced skin wound healing, promoted re-epithelialization, increased collagen synthesis and expression of skin barrier proteins, and reduced inflammation. Using PF-127/hADSCs-Exos complexes allows for lower doses and frequency of exosome administration while maintaining therapeutic effects.
STEM CELL RESEARCH & THERAPY
(2022)
Review
Immunology
Min Jiang, Xupin Jiang, Hongmei Li, Can Zhang, Ze Zhang, Chao Wu, Junhui Zhang, Jiongyu Hu, Jiaping Zhang
Summary: Diabetic foot is a common complication of diabetes and often leads to amputation. Previous studies have shown that stem cell transplantation can promote the healing of chronic diabetic wounds, but safety concerns have limited its clinical application. Recently, it has been discovered that mesenchymal stem cell-derived extracellular vesicles (EVs) have similar therapeutic effects and offer advantages such as safety and reduced immune response. This paper summarizes the current understanding of mesenchymal stem cell-derived EVs in accelerating the healing of diabetic wounds and their potential clinical applications.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Cell & Tissue Engineering
Shune Xiao, Chunfang Xiao, Yong Miao, Jin Wang, Ruosi Chen, Zhexiang Fan, Zhiqi Hu
Summary: The study demonstrated that hAAM scaffolds incorporated with ADSC-Exos can promote diabetic skin wound healing by regulating inflammation, stimulating vascularization, and promoting the production of extracellular matrix.
STEM CELL RESEARCH & THERAPY
(2021)
Review
Biotechnology & Applied Microbiology
Ming Hao, MengNa Duan, Zhijing Yang, Hengzong Zhou, Shuangji Li, Jingcheng Xiang, Han Wu, Huimin Liu, Lu Chang, Dongxu Wang, Weiwei Liu
Summary: Wound healing in the oral and maxillofacial area has a significant impact on the patient's quality of life and mental health. Traditional treatment methods are limited in their efficacy, but exosomes secreted by stem cells have shown promising results. However, natural stem cell exosomes have limitations, and engineered stem cell exosomes have been developed to overcome these limitations and provide targeted promotion of efficient soft tissue repair. These engineered exosomes have anti-inflammatory and cell proliferation regulatory abilities, which make them effective treatments for oral and maxillofacial wound healing.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Review
Biochemistry & Molecular Biology
Chenghai Li, Hua Zhao, Linna Cheng, Bin Wang
Summary: MSC-based therapeutics are available for a range of diseases, with autologous and allogeneic MSCs having their own advantages and disadvantages in medical practice. The interaction of transplanted MSCs with their microenvironment can be impacted by factors from different donors, potentially leading to uncertainty in safety and efficacy. Donor-controlled clinical practice is suggested to minimize potential harm in MSC-based therapies.
CELL AND BIOSCIENCE
(2021)
Review
Biochemistry & Molecular Biology
Guang Yang, Saquib Waheed, Cong Wang, Mehdihasan Shekh, Zhibin Li, Jun Wu
Summary: Exosomes, as therapeutically relevant cell-secreted extracellular vesicles, have garnered immense interest for their role in intercellular communication and wound healing. Stem cell-derived exosomes exhibit similar biological effects to their source cells but with low immunogenicity and no tumorigenicity. They have shown superior efficacy in promoting wound healing by promoting angiogenesis, cell proliferation, and balancing inflammatory responses. Additionally, engineered exosomes and their potential use in combination with materials provide better therapeutic properties not available in traditional single products.
INTERNATIONAL JOURNAL OF BIOLOGICAL SCIENCES
(2023)
Article
Anatomy & Morphology
Wenbo Nie, Xuemiao Huang, Lijing Zhao, Taiwei Wang, Dan Zhang, Tianxin Xu, Lin Du, Yuxiang Li, Weiyuan Zhang, Fengjun Xiao, Lisheng Wang
Summary: The study found that miR-17-92 is highly expressed in both MSCs and MSC-Exos, and that MSC-Exos can promote cutaneous wound healing. Exosomes overexpressing miR17-92 can accelerate cell proliferation, migration, and angiogenesis, while inhibiting ferroptosis. These findings indicate that miR-17-92 plays a role in the repair ability of MSC-Exos, and that exosomes overexpressing miR17-92 may be a new strategy for treating cutaneous wounds.
Article
Cardiac & Cardiovascular Systems
Yongkun Zhan, Xiaolei Sun, Bin Li, Huanhuan Cai, Chen Xu, Qianqian Liang, Chao Lu, Ruizhe Qian, Sifeng Chen, Lianhua Yin, Wei Sheng, Guoying Huang, Aijun Sun, Junbo Ge, Ning Sun
JOURNAL OF MOLECULAR AND CELLULAR CARDIOLOGY
(2018)
Article
Toxicology
Anfeng Cui, Meng Xiang, Ming Xu, Peng Li, Shun Wang, Yajuan Zou, Ke Qiao, Chengyu Jin, Yijun Li, Meng Li, Alex F. Chen, Sifeng Chen
TOXICOLOGY LETTERS
(2019)
Article
Engineering, Biomedical
Hui Yang, Lai Wei, Chen Liu, Weiyi Zhong, Bin Li, Yuncan Chen, Rui Han, Jiexian Zhuang, Jianxun Qu, Hongyue Tao, Haiyan Chen, Chen Xu, Qianqian Liang, Chao Lu, Ruizhe Qian, Sifeng Chen, Wenshuo Wang, Ning Sun
ACTA BIOMATERIALIA
(2019)
Article
Genetics & Heredity
Ruiting Sun, Yingying Liu, Meng Lu, Qianqian Ding, Pingping Wang, Heng Zhang, Xiaoyu Tian, Peng Lu, Dan Meng, Ning Sun, Meng Xiang, Sifeng Chen
JOURNAL OF MOLECULAR MEDICINE-JMM
(2019)
Article
Surgery
Meng Lu, Rong Xue, Pingping Wang, Xiaokai Wang, Xiaoyu Tian, Yingying Liu, Shun Wang, Anfeng Cui, Jingxin Xie, Lili Le, Meng Zhao, Jing Quan, Ning Li, Dan Meng, Xinhong Wang, Ning Sun, Alex F. Chen, Meng Xiang, Sifeng Chen
AMERICAN JOURNAL OF TRANSPLANTATION
(2020)
Article
Cell Biology
Shun Wang, Xiaoyu Tian, Yijun Li, Rong Xue, Haochen Guan, Meng Lu, Huijun Xu, Zhibin Ye, Sifeng Chen, Meng Xiang
OXIDATIVE MEDICINE AND CELLULAR LONGEVITY
(2020)
Article
Cell & Tissue Engineering
Xiaokai Wang, Meng Lu, Xiaoyu Tian, Yansong Ren, Yijun Li, Meng Xiang, Sifeng Chen
STEM CELL RESEARCH & THERAPY
(2020)
Article
Biochemistry & Molecular Biology
Cong Niu, Siqing Wang, Jieyu Guo, Xiangxiang Wei, Mengping Jia, Zhaoxiong Chen, Wenxuan Gong, Yue Qin, Xinhong Wang, Xiuling Zhi, Meng Lu, Sifeng Chen, Mingxia Gu, Jianyi Zhang, Jing-Dong J. Han, Fei Lan, Dan Meng
Summary: This study reveals that the transcription factor BACH1 interacts with OCT4/SOX2/NANOG and MLL/SET1 complexes to maintain pluripotency in mouse embryonic stem cells by recruiting NANOG and MLL/SET1 to chromatin and preserving the trimethylated state of H3K4 to sustain enhancer-promoter activity.
NUCLEIC ACIDS RESEARCH
(2021)
Letter
Cell Biology
Bin Li, Yongkun Zhan, Qianqian Liang, Chen Xu, Xinyan Zhou, Huanhuan Cai, Yufan Zheng, Yifan Guo, Lei Wang, Wenqing Qiu, Baiping Cui, Chao Lu, Ruizhe Qian, Ping Zhou, Haiyan Chen, Yun Liu, Sifeng Chen, Xiaobo Li, Ning Sun
Article
Medicine, General & Internal
Xiuya Li, Fei Gao, Xiaochen Wang, Qianqian Liang, Aobing Bai, Zhuo Liu, Xinyun Chen, Ermin Li, Sifeng Chen, Chao Lu, Ruizhe Qian, Ning Sun, Ping Liang, Chen Xu
Summary: Knockout of the transcription factor E2A increased the proportion of nodal-like cells in hESC-derived CMs, indicating a potential role of E2A in enhancing the efficiency of generating sinoatrial node-like cardiomyocytes from human pluripotent stem cells in the future.
Article
Cell & Tissue Engineering
Yansong Ren, Jinbo Liu, Huijun Xu, Shun Wang, Shirui Li, Meng Xiang, Sifeng Chen
Summary: Knocking out Itgb1 can enhance the migration of iPSCs, promote wound healing, and improve their therapeutic efficacy.
STEM CELL RESEARCH & THERAPY
(2022)
Article
Immunology
Anfeng Cui, Shirui Li, Yijun Li, Dawei Yang, Jiongwei Huang, Xuemeng Wang, Nana Song, Fuchen Chen, Sifeng Chen, Meng Xiang
Summary: The mortality rate of acute lung injury (ALI) and acute respiratory distress syndrome is high. Induced pluripotent stem cell (iPSC) therapy is a potential treatment for ALI, but its efficacy is limited. The study investigated the effect of iPSCs pretreated with NO donors in a mouse model of ALI. The results showed that L-arginine-pretreated iPSCs selectively trafficked into the injured pulmonary tissue and improved lung function and inflammatory response. NO might mediate the therapeutic benefits of iPSCs.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Cell Biology
Xingyu Li, Heng Zhang, Xuemeng Wang, Meng Lu, Qianqian Ding, Alex F. Chen, Meng Xiang, Sifeng Chen
Summary: Exosomes derived from induced pluripotent stem cells (iPSCs) have shown to enhance angiogenesis in naturally aged mice, improve antioxidant capacity and cell proliferation, and reduce aging gene expression. These findings suggest that iPSC-derived exosomes may rejuvenate the vascular system and have anti-aging effects.
Article
Medicine, Research & Experimental
Meng Xiang, Meng Lu, Jing Quan, Ming Xu, Dan Meng, Anfeng Cui, Ning Li, Yingying Liu, Peng Lu, Xueling Kang, Xiaokai Wang, Ning Sun, Meng Zhao, Qiujuan Liang, Lili Le, Xinhong Wang, Jianyi Zhang, Sifeng Chen