Article
Medicine, Research & Experimental
Samantha Wong, Yu Xuan Tan, Abigail Yi Ting Loh, Kiat Yi Tan, Hane Lee, Zainab Aziz, Stanley F. Nelson, Engin Ozkan, Hülya Kayserili, Nathalie Escande-Beillard, Bruno Reversade
Summary: A new lethal syndrome caused by a genetic defect in the proto-oncogene RAF1 is discovered, highlighting the importance of RTK signaling for human development and homeostasis.
EMBO MOLECULAR MEDICINE
(2023)
Article
Environmental Sciences
Yang Xu, Ji Hyun Jang, Myung Chan Gye
Summary: The developmental toxicity of 4-octylphenol (OP) as an estrogenic endocrine disruptor was confirmed in frog embryos, leading to inhibition of head cartilage development and melanocyte generation. OP-induced dysplasia may be attributed to deregulation of neural crest cells in embryos.
ENVIRONMENTAL POLLUTION
(2021)
Article
Cell Biology
Michal Gur, Liat Bendelac-Kapon, Yehuda Shabtai, Graciela Pillemer, Abraham Fainsod
Summary: Retinoic acid (RA) plays a crucial role in regulating multiple developmental decisions during embryogenesis. Excessive RA leads to head malformations, while reduced RA signaling is linked to syndromes exhibiting microcephaly. This study identifies a novel role of RA signaling in the early formation of the head during gastrula stages and demonstrates the importance of RALDH3 in RA production required for head formation.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Multidisciplinary Sciences
Alessandra Dall'Agnese, Jesse M. Platt, Ming M. Zheng, Max Friesen, Giuseppe Dall'Agnese, Alyssa M. Blaise, Jessica B. Spinelli, Jonathan E. Henninger, Erin N. Tevonian, Nancy M. Hannett, Charalampos Lazaris, Hannah K. Drescher, Lea M. Bartsch, Henry R. Kilgore, Rudolf Jaenisch, Linda G. Griffith, Ibrahim I. Cisse, Jacob F. Jeppesen, Tong I. Lee, Richard A. Young
Summary: This study found that insulin receptor (IR) forms dynamic clusters in human hepatocytes and adipocytes. Insulin stimulation promotes further incorporation of IR into these clusters, while in insulin-resistant cells, IR accumulation and dynamic behavior are reduced. Treatment with the commonly used drug metformin, which reduces reactive oxygen species, can rescue IR accumulation and dynamic behavior in insulin-resistant cells. These findings have implications for improved therapeutic approaches.
NATURE COMMUNICATIONS
(2022)
Article
Environmental Sciences
Rong Xu, Yong Huang, Chen Lu, Weiming Lv, Shihua Hong, Shuqin Zeng, Wenyan Xia, Li Guo, Huiqiang Lu, Yijian Chen
Summary: Exposure to Ticlopidine leads to developmental and cardiac toxicity in zebrafish embryos, possibly by increasing oxidative stress. The study highlights the importance of further research on the potential harms of Ticlopidine in humans.
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
(2022)
Article
Biochemistry & Molecular Biology
Gang-Ho Yoon, Kyuhee Kim, Dong-Seok Park, Sun-Cheol Choi
Summary: The negative regulation of RNF152 on the Wnt/beta-catenin pathway during Xenopus early embryogenesis has been discovered. RNF152 can inhibit Wnt signaling by suppressing the polymerization of Dishevelled, which is crucial for Wnt signal transduction, to control neural crest formation in the embryo.
Article
Toxicology
Dou Yang, Juhua Xiao, Mengqi Wan, Jieping Liu, Ling Huang, Xue Li, Li Zhang, Fasheng Liu, Desheng Liang, Yongliang Zheng, Baogang Xie, Xinjun Liao, Guanghua Xiong, Huiqiang Lu, Zigang Cao, Shouhua Zhang
Summary: This study investigated the effects of roxadustat on early embryonic development using zebrafish. The results showed that roxadustat exposure caused abnormal embryonic development and liver metabolic imbalance in zebrafish embryos. Additionally, roxadustat down-regulated Notch signaling, which contributed to hepatotoxicity. This research provides valuable insights for the clinical use of roxadustat.
JOURNAL OF APPLIED TOXICOLOGY
(2023)
Article
Multidisciplinary Sciences
Nydia Tejeda-Munoz, Marco Morselli, Yuki Moriyama, Pooja Sheladiya, Matteo Pellegrini, Edward M. De Robertis
Summary: During canonical Wnt signaling, Wnt induces the endocytosis of focal adhesion proteins and depletion of Integrin beta 1 from the cell surface. This endocytosis leads to changes in the cytoskeleton and is accompanied by the sequestration of Integrin beta 1 within membrane-bounded organelles containing glycogen syntase kinase 3 and focal adhesion-associated proteins.
Article
Biology
Yuichi Murase, Masahiko Yamagishi, Naoyuki Okada, Mika Toya, Junichiro Yajima, Takahiro Hamada, Masamitsu Sato
Summary: Microtubule shortening is crucial for chromosome movement during cell division. In this study, the researchers demonstrated that Dis1, an ortholog of TOG/XMAP215 in fission yeast, plays a role in promoting microtubule shortening. This finding suggests an unconventional function of Dis1 in driving chromosome movement.
COMMUNICATIONS BIOLOGY
(2022)
Article
Developmental Biology
Amanda J. G. Dickinson, Stephen D. Turner, Stacey Wahl, Allyson E. Kennedy, Brent H. Wyatt, Deborah A. Howton
Summary: Environmental teratogens like smoking have been identified as risk factors for developmental disorders, and a new form of smoking called vaping may also pose risks to development. Research has shown that certain chemicals in e-liquids, such as vanillin, may cause craniofacial defects by dysregulating retinoic acid signaling.
DEVELOPMENTAL BIOLOGY
(2022)
Article
Chemistry, Multidisciplinary
Ravindra D. Mule, Akhilesh Kumar, Shashank P. Sancheti, B. Senthilkumar, Himanshu Kumar, Nitin T. Patil
Summary: This study reports the development of a library of benzo[a]quinolizinium-based Au(i) complexes and identifies BQ-AurIPr as a novel redox-active complex with potent anticancer properties. BQ-AurIPr efficiently generates DAMPs and enhances the immunogenicity of cancer cells, making it more effective than FDA-approved drugs known to induce ICD.
Article
Biochemistry & Molecular Biology
Kejie Li, Zhanqi Dong, Feifan Dong, Zhigang Hu, Liang Huang, Jie Wang, Peng Chen, Cheng Lu, Minhui Pan
Summary: Apoptosis is crucial for the normal growth, development, and immunity defense of living organisms. Viral infection triggers apoptosis in infected cells, but viruses have developed countermeasures to inhibit apoptosis in host cells. The study focuses on investigating the mechanism of how IAP2 induces apoptosis as well as the potential for creating resistant material through the knockout of BmNPV iap2.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Biochemistry & Molecular Biology
Qizhi Liu, Stacey Chung, Michael M. Murata, Bingchen Han, Bowen Gao, Maoqi Zhang, Tian-Yu Lee, Evgeny Chirshev, Juli Unternaehrer, Hisashi Tanaka, Armando E. Giuliano, Yukun Cui, Xiaojiang Cui
Summary: Two distinct and diverging cancer cell responses to TOP1 inhibitors were observed in TNBC, with one group being sensitive and the other resistant. The activation patterns of ATR/Chk1 and JNK, as well as the expression of MYC, may serve as predictive markers for cancer cell response to TOP1 inhibitors. The constitutive elevation of MYC expression mediated by JNK activation may represent a novel mechanism governing cancer cell sensitivity to TOP1-targeting therapy.
INTERNATIONAL JOURNAL OF BIOLOGICAL SCIENCES
(2022)
Article
Cell Biology
Alexia Tasca, Martin Helmstaedter, Magdalena Maria Brislinger, Maximilian Haas, Brian Mitchell, Peter Walentek
Summary: The study found that multiciliated cells can undergo apoptosis or trans-differentiate into goblet secretory cells during developmental tissue remodeling, both processes dependent on Notch signaling and modulated by other signaling pathways.
DEVELOPMENTAL CELL
(2021)
Article
Biochemistry & Molecular Biology
Camilla Frattini, Alexy Promonet, Emile Alghoul, Sophie Vidal-Eychenie, Marie Lamarque, Marie-Pierre Blanchard, Serge Urbach, Jihane Basbous, Angelos Constantinou
Summary: The study reveals that TopBP1 self-assembles into functional condensates, which amplify ATR activity and slow down DNA replication. These condensates are reversible in physiological conditions and co-localize with partner proteins.
Article
Biochemical Research Methods
Laura J. Marholz, Joel D. Federspiel, Hyunsuk Suh, Mireia Fernandez Ocana
Summary: This study reports the development of a targeted mass spectrometry-based assay for monitoring various kinases and comparing interspecies variability. Results showed species-specific differences in the kinome of the spleen, especially within certain kinase families. The study also demonstrated the application of these methods in studying species-specific inhibitor profiles.
JOURNAL OF PROTEOME RESEARCH
(2021)
Article
Biochemistry & Molecular Biology
Todd M. Greco, Christopher Secker, Eduardo Silva Ramos, Joel D. Federspiel, Jeh-Ping Liu, Alma M. Perez, Ismael Al-Ramahi, Jeffrey P. Cantle, Jeffrey B. Carroll, Juan Botas, Scott O. Zeitlin, Erich E. Wanker, Ileana M. Cristea
Summary: Huntington disease is a neurodegenerative disorder with multifactorial pathobiology. This study identifies perturbed protein-protein interactions involving the huntingtin protein, suggesting the influence of cellular factors on disease progression. The findings reveal that these interactions are affected by expanded polyglutamine and are associated with impaired synaptic communication and lysosomal acidification.
Article
Multidisciplinary Sciences
Noriyuki Kinoshita, Takamasa S. Yamamoto, Naoko Yasue, Chiyo Takagi, Toshihiko Fujimori, Naoto Ueno
Summary: The study reveals that a tight junction protein ZO-1 forms cytoplasmic condensates in the trophectoderm of mouse embryos during early development. The dynamics of these condensates depend on the physical environment and an interaction between ZO-1 and F-actin.
Article
Cell Biology
Michelle A. Kennedy, Matthew D. Tyl, Cora N. Betsinger, Joel D. Federspiel, Xinlei Sheng, Jesse H. Arbuckle, Thomas M. Kristie, Ileana M. Cristea
Summary: This study developed a mass spectrometry-based method for detecting and quantifying viral proteins in herpesvirus infections. The method was validated and applied in various aspects, providing important insights into viral replication and potential therapeutic agents.
Article
Biology
Manutea C. Serrero, Virginie Girault, Sebastian Weigang, Todd M. Greco, Ana Ramos-Nascimento, Fenja Anderson, Antonio Piras, Ana Hickford Martinez, Jonny Hertzog, Anne Binz, Anja Pohlmann, Ute Prank, Jan Rehwinkel, Rudolf Bauerfeind, Ileana M. Cristea, Andreas Pichlmair, Georg Kochs, Beate Sodeik, Adam P. Geballe
Summary: This study identifies an interferon-inducible protein, MxB, that can sense herpesviral capsids and disassemble them, thereby restricting viral nuclear targeting and progeny capsid assembly, leading to enhanced innate immune responses.
Article
Cell Biology
Hiroshi Koyama, Makoto Suzuki, Naoko Yasue, Hiroshi Sasaki, Naoto Ueno, Toshihiko Fujimori
Summary: Pattern formation and morphogenesis of cell populations are crucial for successful embryogenesis. This theoretical study examined the effects of area elasticity and the coefficient of friction on pattern formation and morphogenesis, revealing that differential cellular stiffness may contribute to tissue elongation within an expanding environment.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Biochemical Research Methods
Michelle A. Kennedy, Todd M. Greco, Bokai Song, Ileana M. Cristea
Summary: Huntington's disease is a progressive neurological disorder caused by polyglutamine expansion of the huntingtin protein. Researchers have developed a web-based platform called HTT-OMNI to visualize and explore potential huntingtin protein interactors and their associated omics measurements.
MOLECULAR & CELLULAR PROTEOMICS
(2022)
Article
Developmental Biology
Yuki Shibata, Miyuki Suzuki, Nao Hirose, Ayuko Takayama, Chiaki Sanbo, Takeshi Inoue, Yoshihiko Umesono, Kiyokazu Agata, Naoto Ueno, Ken-ichi T. Suzuki, Makoto Mochii
Summary: This study establishes a simple targeted transgenesis technique based on CRISPR/Cas9 in Xenopus laevis. By co-injecting Cas9 ribonucleoprotein (RNP) and donor plasmid DNA into Xenopus embryos, approximately 10% of faithful reporter expression can be obtained in F0 crispants, with efficient germline transmission and stable transgene expression observed in the F1 offspring.
DEVELOPMENTAL BIOLOGY
(2022)
Article
Microbiology
Isabelle R. Taylor, Laura A. Murray-Nerger, Todd M. Greco, Dawei Liu, Ileana M. Cristea, Bonnie L. Bassler
Summary: This study reveals specific protein interactions involving PqsE and the role of RhlR in these interactions. These findings contribute to the understanding of the pathogenic mechanisms and quorum sensing process in Pseudomonas aeruginosa.
Article
Multidisciplinary Sciences
Kodihalli C. Ravindra, Vishal S. Vaidya, Zhenyu Wang, Joel D. Federspiel, Richard Virgen-Slane, Robert A. Everley, Jane I. Grove, Camilla Stephens, Mireia F. Ocana, Mercedes Robles-Diaz, M. Isabel Lucena, Raul J. Andrade, Edmond Atallah, Alexander L. Gerbes, Sabine Weber, Helena Cortez-Pinto, Andrew J. Fowell, Hyder Hussaini, Einar S. Bjornsson, Janisha Patel, Guido Stirnimann, Sumita Verma, Ahmed M. Elsharkawy, William J. H. Griffiths, Craig Hyde, James W. Dear, Guruprasad P. Aithal, Shashi K. Ramaiah
Summary: Diagnosis and distinction of drug-induced liver injury (DILI) from other liver diseases pose significant challenges. This study identifies and validates potential biomarkers for diagnosing DILI and distinguishing it from alternative causes of liver injury. These biomarkers show promising performance characteristics in distinguishing DILI patients from healthy volunteers and patients with non-DILI liver injury.
NATURE COMMUNICATIONS
(2023)
Article
Biology
Arian Mansur, Remi Joseph, Euri S. Kim, Pierre M. Jean-Beltran, Namrata D. Udeshi, Cadence Pearce, Hanjie Jiang, Reina Iwase, Miroslav P. Milev, Hashem A. Almousa, Elyshia McNamara, Jeffrey Widrick, Claudio Perez, Gianina Ravenscroft, Michael Sacher, Philip A. Cole, Steven A. Carr, Vandana A. Gupta
Summary: Dysfunction of the ubiquitin-proteasome system (UPS) is associated with various human diseases. This study focuses on understanding the regulation of protein turnover during skeletal muscle development and disease progression. By analyzing the KLHL40-regulated ubiquitin-modified proteome in zebrafish, the researchers identified the role of KLHL40 in ER-Golgi anterograde trafficking and its impact on muscle development. The findings suggest that the muscle proteome is finely regulated by ubiquitylation and uncover new disease mechanisms.
Correction
Endocrinology & Metabolism
Jeremy M. Robbins, Bennet Peterson, Daniela Schranner, Usman A. Tahir, Theresa Rienmuller, Shuliang Deng, Michelle J. Keyes, Daniel H. Katz, Pierre M. Jean Beltran, Jacob L. Barber, Christian Baumgartner, Steven A. Carr, Sujoy Ghosh, Changyu Shen, Lori L. Jennings, Robert Ross, Mark A. Sarzynski, Claude Bouchard, Robert E. Gerszten
Article
Endocrinology & Metabolism
Jeremy M. Robbins, Bennet Peterson, Daniela Schranner, Usman A. Tahir, Theresa Rienmueller, Shuliang Deng, Michelle J. Keyes, Daniel H. Katz, Pierre M. Jean Beltran, Jacob L. Barber, Christian Baumgartner, Steven A. Carr, Sujoy Ghosh, Changyu Shen, Lori L. Jennings, Robert Ross, Mark A. Sarzynski, Claude Bouchard, Robert E. Gerszten
Summary: The study analyzed plasma protein levels and identified proteins associated with individual oxygen consumption and increase in oxygen consumption after exercise training, which improved the prediction of individual oxygen consumption. The findings were validated in separate exercise cohorts and linked some proteins to all-cause mortality in a community-based cohort. The study also reproduced the specificity of key findings using antibody-based assays.