Article
Multidisciplinary Sciences
Oliver F. Harder, Sarah V. Barrass, Marcel Drabbels, Ulrich J. Lorenz
Summary: Microsecond time-resolved cryo-EM allows for observation of fast protein dynamics, as demonstrated by the contraction of the CCMV capsid induced by a pH jump.
NATURE COMMUNICATIONS
(2023)
Review
Biophysics
Doeke R. Hekstra
Summary: Proteins play crucial roles in guiding the flow of information, energy, and matter in life by accelerating transport and chemical reactions, modulating these reactions, and forming dynamic supramolecular assemblies. Conformational change is fundamental to functional transitions, and time-resolved X-ray diffraction methods have been successful in characterizing these transitions. This review focuses on emerging techniques that investigate the dynamic basis of function in proteins without native light-dependent transitions, and discusses potential extensions and future possibilities. Additionally, the review examines the challenges posed by the weaker and more distributed signals in these data, pushing the limits of analytical methods. Overall, these new methods are establishing a powerful paradigm for studying the physics of protein function.
ANNUAL REVIEW OF BIOPHYSICS
(2023)
Review
Multidisciplinary Sciences
Gisela Branden, Richard Neutze
Summary: Conformational changes within biological macromolecules regulate chemical reactions in living cells. Time-resolved crystallography can reveal these changes in detail, while serial crystallography approaches are now being routinely used for studying macromolecules. Recent progress includes visualizing ultrafast and slower time-scale structural changes in proteins like bacteriorhodopsin and photosystem II.
Article
Chemistry, Multidisciplinary
Sylvain Aumonier, Sylvain Engilberge, Nicolas Caramello, David von Stetten, Guillaume Gotthard, Gordon A. Leonard, Christoph Mueller-Dieckmann, Antoine Royant
Summary: The development of serial crystallography has revitalized room-temperature macromolecular crystallography. This study describes the room-temperature structural study of a reaction intermediate using a synchrotron. The authors were able to monitor the relaxation process of the intermediate and discovered unexpected protein rearrangements.
Article
Chemistry, Multidisciplinary
Adam Czajka, Steven P. Armes
Summary: The study investigated the aqueous emulsion polymerization of TFEMA using persulfate initiation and monitored the particle size evolution using SAXS and DLS. Well-defined spherical latexes were formed under different conditions, with significant differences observed between surfactant-free and SDS formulations. Comparable polymerization kinetics were observed for in situ SAXS experiments and laboratory-scale syntheses.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2021)
Article
Multidisciplinary Sciences
Ramsay Macdonald, Brendan J. Mahoney, Jess Soule, Andrew K. Goring, Jordan Ford, Joseph A. Loo, Duilio Cascio, Robert T. Clubb
Summary: Streptococcus pyogenes uses the Shr receptor to capture hemoglobin and acquire its iron-laden heme molecules. Through a cap and release mechanism facilitated by the HID1 and HID2 domains, Shr preferentially recognizes heme-loaded forms of hemoglobin and transfers the heme to NEAT domains for iron acquisition. These receptor dynamics maximize heme scavenging efficiency by selectively targeting heme-loaded forms of hemoglobin.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Article
Chemistry, Multidisciplinary
Jonathan A. Clinger, David W. Moreau, Matthew J. McLeod, Todd Holyoak, Robert E. Thorne
Summary: Millisecond mix-and-quench crystallography (MMQX) has been developed to achieve millisecond time-resolved data using fewer crystals and routine remote synchrotron data collection. This approach lowers the barrier to time-resolved crystallography and enables a broad expansion in structural studies of protein dynamics.
Article
Multidisciplinary Sciences
M. Wilamowski, D. A. Sherrell, Y. Kim, A. Lavens, R. W. Henning, K. Lazarski, A. Shigemoto, M. Endres, N. Maltseva, G. Babnigg, S. C. Burdette, V. Srajer, A. Joachimiak
Summary: Serial x-ray crystallography can reveal binding events and chemical conversions during enzymatic reactions. This study used time-resolved serial synchrotron crystallography to analyze the structure, binding, and cleavage of moxalactam antibiotic in L1 metallo-beta-lactamase from Stenotrophomonas maltophilia.
NATURE COMMUNICATIONS
(2022)
Article
Physics, Multidisciplinary
Can B. Uzundal, Sasawat Jamnuch, Emma Berger, Clarisse Woodahl, Paul Manset, Yasuyuki Hirata, Toshihide Sumi, Angelique Amado, Hisazumi Akai, Yuya Kubota, Shigeki Owada, Kensuke Tono, Makina Yabashi, John W. Freeland, Craig P. Schwartz, Walter S. Drisdell, Iwao Matsuda, Tod A. Pascal, Alfred Zong, Michael Zuerch
Summary: Second harmonic generation (SHG) spectroscopy is commonly used to investigate surface chemistry, interfacial chemistry, and symmetry properties in solids, but the complexity of novel materials makes interpretation of experiments difficult. Polarization-resolved extreme ultraviolet (XUV-SHG) spectroscopy demonstrates element-specific angular anisotropy investigations for the first time, paving the way for future angle and time-resolved XUV-SHG studies with elemental specificity in condensed matter systems.
PHYSICAL REVIEW LETTERS
(2021)
Article
Chemistry, Multidisciplinary
Hongjie Li, Yoshiki Nakajima, Takashi Nomura, Michihiro Sugahara, Shinichiro Yonekura, Siu Kit Chan, Takanori Nakane, Takahiro Yamane, Yasufumi Umena, Mamoru Suzuki, Tetsuya Masuda, Taiki Motomura, Hisashi Naitow, Yoshinori Matsuura, Tetsunari Kimura, Kensuke Tono, Shigeki Owada, Yasumasa Joti, Rie Tanaka, Eriko Nango, Fusamichi Akita, Minoru Kubo, So Iwata, Jian-Ren Shen, Michihiro Suga
Summary: The study successfully captured the structural dynamics of light-sensitive proteins during the S-1 to S-2 transition using pump-probe time-resolved serial femtosecond crystallography (TR-SFX), while also avoiding the influence of light contamination, revealing key structural changes important for the water-splitting reaction.
Article
Engineering, Electrical & Electronic
Sara Pettinato, Marco Girolami, Riccardo Olivieri, Antonella Stravato, Cristina Caruso, Stefano Salvatori
Summary: This study presents a diamond dosimeter coupled to compact front-end electronics for accurate dose measurements and real-time beam monitoring. The system demonstrates good stability and accuracy, and shows potential for source diagnostics in terms of beam intensity and emission timing.
IEEE SENSORS JOURNAL
(2022)
Article
Multidisciplinary Sciences
Takashi Nomura, Tetsunari Kimura, Yusuke Kanematsu, Daichi Yamada, Keitaro Yamashita, Kunio Hirata, Go Ueno, Hironori Murakami, Tamao Hisano, Raika Yamagiwa, Hanae Takeda, Chai Gopalasingam, Ryota Kousaka, Sachiko Yanagisawa, Osami Shoji, Takashi Kumasaka, Masaki Yamamoto, Yu Takano, Hiroshi Sugimoto, Takehiko Tosha, Minoru Kubo, Yoshitsugu Shiro
Summary: The study investigated the mechanism of NO reductase catalyzing the generation of N2O. Time-resolved techniques and photolabile-caged NO were used to characterize the structure and formation of the intermediate I in NO reductase. Quantum mechanics/molecular mechanics calculations and experimental results revealed the electronic structure characteristics of the intermediate I.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Chemistry, Physical
Key Young Oang, Sungjun Park, Jiwon Moon, Eunji Park, Hyun Kyung Lee, Tokushi Sato, Shunsuke Nozawa, Shin-ichi Adachi, Joonghan Kim, Jeongho Kim, Jeong-Hun Sohn, Hyotcherl Ihee
Summary: Time-resolved X-ray liquidography (TRXL) is a powerful technique for studying the structural dynamics of small molecules and macromolecules in liquid solutions. However, it has limited sensitivity for small molecules containing light atoms only, making the signal from solvent molecules dominate. This study presents an alternative approach using solvent temperature change to detect the dynamics of photoinduced reactions, allowing the identification of intermediates and reaction pathways in small molecules without heavy atoms.
JOURNAL OF PHYSICAL CHEMISTRY LETTERS
(2023)
Article
Physics, Applied
H. B. Radousky, M. R. Armstrong, N. Goldman
Summary: The availability of pulsed X rays on short timescales has opened up new avenues of research in the physics and chemistry of shocked materials, while theoretical advancements have increased the predictive capability of models. This paper discusses recent experimental results and developments in theory and modeling to address complex situations, as well as the potential impact of new platforms and increased beam time on the future direction of the field.
JOURNAL OF APPLIED PHYSICS
(2021)
Review
Cell Biology
Pieter-Jan Vermeire, Giel Stalmans, Anastasia Lilina, Jan Fiala, Petr Novak, Harald Herrmann, Sergei Strelkov
Summary: IFs play a crucial role in normal cell physiology and are linked to numerous diseases, highlighting the importance of understanding their molecular structure. While progress has been made in studying the IF structure, many mysteries and challenges remain. In addition to X-ray crystallography, chemical cross-linking and cryoelectron microscopy are expected to lead to major advancements in the field in the near future.