Article
Engineering, Biomedical
Zehong Chen, Jiancheng Zhai, Jiahui Ma, Peng Chen, Weishun Lin, Weipeng Zhang, Jiaming Xiong, Chaowei Zhang, Hongbo Wei
Summary: The study investigates the effects of melatonin on the therapeutic effects of small extracellular vesicles (sEVs) derived from mesenchymal stem cells (MSCs) in the treatment of cavernous nerve injury (CNI) erectile dysfunction (ED). The results show that melatonin-pretreated sEVs significantly improve erectile function and reduce phenotypic modulation in CNI ED rats. The therapeutic effects of melatonin-pretreated sEVs are superior to untreated sEVs. The underlying mechanism involves the enrichment of miR-10a-3p in melatonin-pretreated sEVs, which targets the protein kinase inhibitor alpha (PKIA). The study suggests that melatonin-pretreated sEVs could be potential therapeutic vectors or bioactive materials for CNI ED.
ADVANCED HEALTHCARE MATERIALS
(2023)
Article
Medicine, Research & Experimental
Zhaoer Yu, Wei Zhang, Yixiao Wang, Mingming Gao, Min Zhang, Dan Yao, Chengping Qiao, Xianwei Cui, Ruizhe Jia
Summary: HUMSCs-derived extracellular vesicles can ameliorate endothelial dysfunction in preeclampsia by activating the arginine metabolic pathway and may serve as a therapeutic method for treating preeclampsia.
MOLECULAR PHARMACEUTICS
(2023)
Review
Endocrinology & Metabolism
Dao-Sheng Luo, Yan-Qing Li, Zhi-Quan Deng, Gui-Hua Liu
Summary: The review focuses on the current progress in the clinical application of SC treatment for DED, and summarizes the development prospects of SCs in the field of DMED therapy.
WORLD JOURNAL OF DIABETES
(2021)
Article
Agriculture, Multidisciplinary
Tianyuan Song, Miao Lv, Minzhi Zhou, Mingtao Huang, Lin Zheng, Mouming Zhao
Summary: The study explored the effect of EVs produced from VSMCs on HUVECs, revealing differential gene expression associated with endothelial dysfunction. LSW can improve endothelial function by repairing miRNA expression in VSMCs, suggesting potential mechanisms for the actions of Ang II and LSW on vascular endothelial cells.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2021)
Article
Medicine, Research & Experimental
Qihua Wang, Xiangyu Chen, Congzhe Ren, Zhunan Xu, Yang Pan, Shangren Wang, Xiaoqiang Liu
Summary: Diabetes mellitus erectile dysfunction (DMED) is a common chronic disease, and stem cell treatment has the potential to improve this condition. Artificial extracellular matrix (ECM) and low intensity pulsed ultrasound (LIPUS) can enhance the therapeutic effect of stem cells. The combination of these technologies may be a promising direction for DMED treatment.
MEDICAL HYPOTHESES
(2023)
Review
Immunology
Xi Liu, Qian Wei, Lu Lu, Shengnan Cui, Kui Ma, Wenhua Zhang, Fang Ma, Haihong Li, Xiaobing Fu, Cuiping Zhang
Summary: Inflammatory diseases caused by immune system disorders are a major public health concern. Mesenchymal stem cell derived extracellular vesicles (MSC-EVs) have shown great potential in immune modulation and offer a promising solution for the treatment of inflammatory diseases. Despite being in the early stages of research, MSC-EVs have been found to regulate the activities of innate and adaptive immune cells and have been tested in clinical trials for inflammatory diseases.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Andrology
Jingming Zhuang, Peng Gao, Haoran Chen, Zujun Fang, Jie Zheng, Daqian Zhu, Jiangang Hou
Summary: This study demonstrated the effectiveness of topical application of USC-EVs-HA in the treatment of DM2ED rats. The potential mechanism may involve promoting the proliferation of endothelial cells and smooth muscle in the corpus cavernosum, leading to the remodeling of erectile function. Multiple dosing at intervals may enhance the effect.
Article
Cardiac & Cardiovascular Systems
Chao Wang, Cong Liu, Jiaxin Shi, Hairu Li, Shuangquan Jiang, Peng Zhao, Maomao Zhang, Guoqing Du, Shuai Fu, Shouqiang Li, Zhuo Wang, Xiaokun Wang, Fei Gao, Ping Sun, Jiawei Tian
Summary: Nicotine, a major component of tobacco, aggravates atherosclerosis by increasing extracellular vesicles (EVs) from smokers, which exacerbates endothelial inflammation and apoptosis. The levels of miR-155 in smoker-EVs were significantly increased and correlated with carotid plaque formation. Monocytes were identified as an important source of smoker-EVs, and the increased levels of miR-155 in monocytes may be regulated by DNA methylation and the transcription factor HIF1 alpha. Mechanistically, EVs encapsulated miR-155 induced endothelial cell dysfunction by targeting BCL2, MCL1, TIMP3, BCL6, and activating the NF-kappa B pathway.
CARDIOVASCULAR RESEARCH
(2023)
Article
Biochemistry & Molecular Biology
Dayane Mayumi Miyasaki, Alexandra Cristina Senegaglia, Sergio Adriane Bezerra de Moura, Amanda Leitolis, Luiz Guilherme Achcar Capriglione, Leticia Fracaro, Lidiane Maria Boldrini Leite, Paulo Henrique Utumi, Felipe Yukio Ishikawa Fragoso, Fernando Meyer, Alejandro Correa, Paulo Roberto Slud Brofman
Summary: This study evaluated the effects of extracellular vesicles (EVs) from human umbilical cord tissue and human umbilical cord blood on rats with chronic kidney disease. The results demonstrated that EVs have the potential to improve renal function, suggesting their use as a cell-free treatment.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Biochemistry & Molecular Biology
Gianmaria Salvio, Alessandro Ciarloni, Melissa Cutini, Giancarlo Balercia
Summary: Erectile Dysfunction (ED) is linked to cardiovascular conditions, with hyperhomocysteinemia playing a central role in causing endothelial damage and affecting male sexual function.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Biochemistry & Molecular Biology
Sevindzh Kletukhina, Guzel Mutallapova, Angelina Titova, Marina Gomzikova
Summary: Idiopathic pulmonary fibrosis (IPF) is a progressive disease caused by the aberrant regeneration of pulmonary epithelium. Mesenchymal stem cells (MSCs) and extracellular vesicles (EVs) are promising therapies for pulmonary fibrosis.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Pharmacology & Pharmacy
Jean Michell Santoyo, Jose Antonio Noguera, Francisco Aviles, Trinidad Hernandez-Caselles, Catalina de Paco-Matallana, Juan Luis Delgado, Santiago Cuevas, M. Teresa Llinas, Isabel Hernandez
Summary: This study aimed to assess the effects of pravastatin on the generation of circulating extracellular vesicles in women at high risk of term preeclampsia. The results showed that pravastatin can reduce the levels of cell-derived membrane vesicles in the circulation of high-risk preeclampsia patients, which may help reduce the characteristics of endothelial dysfunction, inflammation, and other features of the disease.
FRONTIERS IN PHARMACOLOGY
(2023)
Article
Cell Biology
Ling Wang, Shiyu Liu, Ka Li, Aijin Ma, Chenghu Hu, Changlin Wang, Nan Cao, Yunpeng Zhao, Ruifeng Fu, Wenwen Jia, Peng Xiang, Houqi Liu, Zhongquan Qi, Ningwen Zhu, Lingmin Liang, Lei Wang, Jiani Cao, Peijun Zhai, Jiaxi Zhou, Jun Wei, Tao Na, Jun Wu, Zhiying He, Guangdong Zhou, Weifeng Yu, Jinyan Wu, Wen Zeng, Yong Zhang, Lijun Zhu, Boqiang Fu, Jingzhong Zhang, Shuwei Yang, Chengxiang Dai, Hengmi Cui, Jianzhong Jing, Hexin Yan, Xiaowen He, Yongbo Lu, Cailing Tong, Tongbiao Zhao, Jie Hao, Xialin Liu, Yan Jin, Yue Wang
Summary: This article introduces the general requirements for the production of extracellular vesicles derived from human stem cells in China, aiming to promote standardization in research and production in this field and accelerate international standardization.
CELL PROLIFERATION
(2023)
Article
Chemistry, Medicinal
Sang Hong Bak, Jae Heon Kim, Seung U. Kim, Dong-Seok Lee, Yun Seob Song, Hong J. Lee
Summary: This study aimed to establish immortalized rat penile cavernous endothelial cells (rEC) and investigate how they could repair erectile dysfunction in rats with cavernous nerve injury (CNI). The findings showed that transfected rECs expressed CD31 and vWF consistently and were able to repair erectile dysfunction caused by CNI. Therefore, this cell line may be valuable for studying the mechanisms and drug screening of erectile dysfunction in rats.
Review
Cell & Tissue Engineering
Senthilkumar Alagesan, Jack Brady, Declan Byrnes, Juan Fandino, Claire Masterson, Sean McCarthy, John Laffey, Daniel O'Toole
Summary: Cell therapy, particularly mesenchymal stem/stromal (MSC) therapy, has been explored for various diseases, especially those with inflammation. However, recent studies have shown that MSC is not a cure-all. This review discusses current and future strategies to overcome limitations in MSC efficacy, including inspirations from stem cell niche and previous gene therapy work. It also explores the emerging field of extracellular vesicle therapy and enhancement protocols.
STEM CELL RESEARCH & THERAPY
(2022)