Article
Biochemistry & Molecular Biology
Hiba Abu Hariri, Ilana Braunstein, Talal Salti, Fabian Glaser, Tal Gefen, Naama Geva-Zatorsky, Tamar Ziv, Moran Benhar
Summary: This study characterized the dynamic cysteine proteome of human macrophages and identified the role of thiol redox control in regulating inflammatory responses. The findings provide insights into the adaptability of macrophages to changing inflammatory and redox environments.
ANTIOXIDANTS & REDOX SIGNALING
(2023)
Review
Biochemistry & Molecular Biology
Roshan Kumar, Ruma Banerjee
Summary: The article discusses two main regulatory mechanisms of H2S on redox homeostasis: one is the impact on cellular effects through protein persulfidation, and the other is the influence on major redox nodes through remodeling the redox metabolome. These regulatory mechanisms help protect the thiol proteome from oxidative damage.
CRITICAL REVIEWS IN BIOCHEMISTRY AND MOLECULAR BIOLOGY
(2021)
Review
Biochemistry & Molecular Biology
Xiaolu Li, Tong Zhang, Nicholas J. J. Day, Song Feng, Matthew J. J. Gaffrey, Wei-Jun Qian
Summary: Protein S-glutathionylation (SSG) is a reversible post-translational modification that plays a critical role in redox signaling and regulation. This review summarizes current biochemical and analytical approaches for characterizing SSG and highlights recent examples of the functional and structural consequences of SSG modifications. The article also discusses future directions for research and the potential of computational methods in accelerating functional discovery.
Article
Multidisciplinary Sciences
Claudia Montllor-Albalate, Hyojung Kim, Anna E. Thompson, Alex P. Jonke, Matthew P. Torres, Amit R. Reddi
Summary: Cu/Zn superoxide dismutase (Sod1) acts as an important antioxidant enzyme, promoting NADPH production and balancing glycolytic and oxPPP flux by controlling GAPDH activity to adapt to life in air. Sod1-derived H2O2 is crucial for antioxidant defense and serves as a master regulator of metabolism and the thiol redoxome, impacting numerous metabolic enzymes.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Plant Sciences
Liangqian Yu, Sidra Iqbal, Yuting Zhang, Guofang Zhang, Usman Ali, Shaoping Lu, Xuan Yao, Liang Guo
Summary: The study found that a large number of proteins in Brassica napus undergo sulphenylation during salt stress, with many proteins localizing to chloroplasts and cytoplasm and enzyme activity affected. Differential sulphenylated proteins indicate that metabolic processes such as photosynthesis and glycolysis play important roles in the response.
PLANT CELL AND ENVIRONMENT
(2021)
Article
Biochemistry & Molecular Biology
Marisa L. Henry, Deborah Velez-Irizarry, Joe D. Pagan, Lorraine Sordillo, Jeff Gandy, Stephanie J. Valberg
Summary: The study found that oral coenzyme Q10 and N-acetyl-cysteine supplementation significantly increased muscle glutathione concentrations in horses after intense exercise, while also positively impacting mitochondrial proteins.
Article
Chemistry, Multidisciplinary
Wenjie Tao, Curtis E. Moore, Shiyu Zhang
Summary: A redox-neutral S-nitrosation of thiol has been achieved at a dicopper(I,I) center, providing new insights into the mechanism of S-nitrosation. The reaction yielded thiol and di-S-nitrosothiol complexes, indicating that S-nitrosation proceeds through similar intermediate species.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2021)
Article
Microbiology
Xinrong Zhang, Zhenhua Zhang, Xiao-Lin Chen
Summary: Redox modification plays significant roles in plant pathogenic fungi, impacting various biological and cellular functions, such as fungal growth and pathogenicity. The global thiol proteome survey in Magnaporthe oryzae provides valuable insights for future studies on redox modification in fungi.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Vitor S. Almeida, Lara L. Miller, Joao P. G. Delia, Augusto Magalhaes, Icaro P. Caruso, Anwar Iqbal, Fabio C. L. Almeida
Summary: In this study, the mechanism of S-(trans)nitrosation of thioredoxin (hTrx) by nitric oxide (NO) was investigated using NMR spectroscopy and mass spectrometry. The reactivity of each of the five cysteines of hTrx was measured, revealing that C73 is the most reactive and responsible for S-transnitrosation to other cellular targets. Internal transfers of NO were observed leading to S-nitrosation of C62, which serves as a storage site for NO. Additionally, C69-SNO only forms under nitrosative stress and leads to nuclear translocation of hTrx.
Article
Biochemistry & Molecular Biology
Fernando Martin Baidanoff, Laura Lucia Trebucq, Santiago Andres Plano, Phillip Eaton, Diego Andres Golombek, Juan Jose Chiesa
Summary: This study investigated the oxidation of cysteine thiols in the circadian protein period 2 (PER2) in HEK-293T cells. The results showed that cysteine oxidation can alter the stability and form of PER2. These findings reveal the importance of cysteine oxidation in the redox regulation of the molecular circadian clock.
Article
Plant Sciences
Amanda L. Smythers, Nikita Bhatnagar, Chien Ha, Parinita Majumdar, Evan W. McConnell, Boominathan Mohanasundaram, Leslie M. Hicks, Sona Pandey
Summary: This study investigates the role of reversible protein oxidation in plant stress response and identifies a complex network of reversible oxidations associated with ABA and G-protein signaling. The findings indicate that functional G-proteins are required to maintain intracellular redox homeostasis and fully execute plant stress responses.
Review
Biochemical Research Methods
Xiaolu Li, Austin Gluth, Tong Zhang, Wei-Jun Qian
Summary: Redox post-translational modifications greatly impact protein structure and function, allowing for the regulation of biological processes. Redox proteomics approaches use mass spectrometry to study these modifications and have contributed to our understanding of redox signaling and regulation. Various strategies, including blocking free thiols and using selective reduction and enrichment techniques, are employed to analyze redox PTMs. This review discusses the principles and recent advances in redox proteomics and highlights its applications in generating novel biological insights.
Article
Plant Sciences
Magdalena Fuessl, Ann-Christine Koenig, Jurgen Eirich, Markus Hartl, Laura Kleinknecht, Alexandra-Viola Bohne, Anne Harzen, Katharina Kramer, Dario Leister, Joerg Nickelsen, Iris Finkemeier
Summary: This study identified dynamic changes in protein lysine acetylation in Chlamydomonas under different growth conditions, particularly in enzymes related to photosynthesis, fatty acid metabolism, and the glyoxylate cycle depending on acetate and light. The newly discovered lysine acetylation sites may be valuable for genetic engineering of metabolic pathways in Chlamydomonas.
Article
Microbiology
Yun Lu, Hongtong Chen, Penghe Wang, Jing Pang, Xi Lu, Guoqing Li, Xinxin Hu, Xiukun Wang, Xinyi Yang, Congran Li, Yu Lu, Xuefu You
Summary: The study investigates the modifications of cysteine residues in the proteome of Mycobacterium tuberculosis under oxidative stress, revealing proteins that play a role in maintaining redox homeostasis.
MICROBIOLOGY SPECTRUM
(2023)
Article
Cell Biology
Yangmiao Duan, Jingyi Li, Fengqin Wang, Junmin Wei, Zhongfa Yang, Mingxin Sun, Jia Liu, Mingxin Wen, Wan Huang, Zhinan Chen, Zhimin Lu, Jing-Hua Yang, Guangwei Wei
Summary: This study identifies dysregulated modifications on a range of enzymes related to the glycolysis pathway, including the importance of TPI Ser58 phosphorylation in glycolysis and lung cancer development.
Article
Biochemical Research Methods
Maria-Angeles Castillejo, Sara Fondevilla-Aparicio, Carlos Fuentes-Almagro, Diego Rubiales
JOURNAL OF PROTEOME RESEARCH
(2020)
Article
Biochemistry & Molecular Biology
Jose A. Martina, David Guerrero-Gomez, Eva Gomez-Orte, Jose Antonio Barcena, Juan Cabello, Antonio Miranda-Vizuete, Rosa Puertollano
Summary: Mammalian TFEB and TFE3, as well as their ortholog in Caenorhabditis elegans HLH-30, are regulated by a novel mechanism involving cysteine-mediated redox switch under stress conditions, leading to oligomer formation and increased stability to stress. Cysteine oxidation acts as a molecular switch linking redox balance changes with expression of target genes.
Article
Biochemistry & Molecular Biology
Rafael Carrasco-Reinado, Almudena Escobar-Nino, Carlos Fajardo, Ines M. Morano, Francisco Amil-Ruiz, Gonzalo Martinez-Rodriguez, Carlos Fuentes-Almagro, Victoria Capilla, Lidia Tomas-Cobos, Laura Soriano-Romani, Palmira Guarnizo, Roberto A. Vallejo, Francisco Javier Fernandez-Acero
Summary: Proteomics plays a key role in understanding molecular dynamics of biological processes, while the combination of bioinformatics tools in applied proteomics can identify proteins with potential industrial applications, like UCA01 against cancer, and new biomolecule sources from Nannochloropsis gaditana.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biotechnology & Applied Microbiology
Francisco Amil-Ruiz, Ana Maria Herruzo-Ruiz, Carlos Fuentes-Almagro, Casimiro Baena-Angulo, Jose Manuel Jimenez-Pastor, Julian Blasco, Jose Alhama, Carmen Michan
Summary: This study focused on analyzing the molecular mechanisms of pollutant responses in the bivalve species Scrobicularia plana by creating a robust reference transcriptome and proteome database. Through a comprehensive approach, a high-quality assembly was selected and proteomic analysis revealed a wide coverage of molecular functions and biological processes, confirming the strength of the generated database.
Article
Engineering, Biomedical
E. Giraldo, V. J. Nebot, S. Dordevic, R. Requejo-Aguilar, A. Alastrue-Agudo, O. Zagorodko, A. Arminan, B. Martinez-Rojas, M. J. Vicent, V. Moreno-Manzano
Summary: Research shows that improving the stability of fasudil by conjugating it with polymers in a bioresponsive manner can enhance its therapeutic potential in treating spinal cord injuries. The conjugate PGA-SS-F, designed based on the conditions after SCI, significantly promotes neurite elongation and axon growth, reduces early apoptosis, and enhances cell engraftment in a rat model of SCI. This synergistic therapeutic approach may offer a promising clinical strategy for SCI treatment.
Article
Microbiology
Ana M. Herruzo-Ruiz, Carlos A. Fuentes-Almagro, Jose M. Jimenez-Pastor, Victor M. Perez-Rosa, Julian Blasco, Carmen Michan, Jose Alhama
Summary: This study assessed the utility of soil's metaomics for environmental diagnosis in Donana National Park, finding lower bacterial abundance and diversity in areas historically exposed to contamination surrounding the park. Metaproteomics analysis identified significant alterations in global biological processes in response to environmental changes.
ENVIRONMENTAL MICROBIOLOGY
(2021)
Article
Cell Biology
Ana Soriano-Arroquia, John Gostage, Qin Xia, David Bardell, Rachel McCormick, Eugene McCloskey, Ilaria Bellantuono, Peter Clegg, Brian McDonagh, Katarzyna Goljanek-Whysall
Summary: The decline in satellite cell-dependent skeletal muscle regeneration with aging is associated with alterations in miR-24 expression and its target gene Prdx6. Changes in miR-24 levels impact mitochondrial ROS generation, DNA damage pathways, and the viability and myogenic potential of myogenic progenitors, with more pronounced effects observed in old mice. The downregulation of miR-24 and subsequent upregulation of Prdx6 in response to aging may represent an adaptive mechanism to maintain satellite cell function in muscle.
Article
Cell Biology
Maria Jose Lopez-Grueso, Carmen Alicia Padilla, Jose Antonio Barcena, Raquel Requejo-Aguilar
Summary: The multifunctional protein DJ-1 is involved in the development of Parkinson's disease through disruption of cell cycle checkpoints. It leads to accumulation of p-tau and alpha-synuclein proteins, alteration of PI3K/AKT and MAPK signaling pathways, and deregulation of Cdk5. The findings suggest that aborted cell cycle re-entry could be the trigger for DJ-1-associated Parkinson's disease, and targeting cell cycle re-entry may improve therapeutic strategies.
CELLULAR AND MOLECULAR NEUROBIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Juan Luis Penas-Fuentes, Eva Siles, Eva E. Rufino-Palomares, Amalia Perez-Jimenez, Fernando J. Reyes-Zurita, Jose A. Lupianez, Carlos Fuentes-Almagro, Juan Peragon-Sanchez
Summary: This study characterized the cytotoxic effects of erythrodiol (EO) on human hepatocarcinoma cells. EO decreased cell viability and antioxidant defense systems, impaired important metabolic processes and nuclear transport, but induced AMP biosynthesis and cell cycle G2/M phase transition.
Review
Cell Biology
Raquel Requejo-Aguilar
Summary: Neurodegenerative diseases are caused by the progressive loss of specific neurons. The exact mechanisms of action of these diseases are unknown, but aberrant cell cycle re-entry seems to play a major role. Factors such as oxidative stress, DNA damage response, signaling pathway disturbance, and ubiquitin proteasome malfunction contribute to cell cycle re-entry in post-mitotic neurons, with Cdk5 being a key protein involved.
NEURAL REGENERATION RESEARCH
(2023)
Article
Biochemistry & Molecular Biology
Qin Xia, Jose C. Casas-Martinez, Eduardo Zarzuela, Javier Munoz, Antonio Miranda-Vizuete, Katarzyna Goljanek-Whysall, Brian McDonagh
Summary: Exercise increases Reactive Oxygen Species (ROS) in muscle, promoting changes in gene transcription and mitochondrial biogenesis. Peroxiredoxin 2 (Prdx2) is necessary for the adaptive response to physiological levels of H2O2 and exercise. H2O2 improves mitochondrial capacity and myogenesis in myoblasts, which is suppressed when Prdxs expression is decreased. Swimming exercise in C. elegans enhances mitochondrial content, fitness, survival, and longevity, but these benefits are diminished in prdx-2 mutant worms. Prdx-2 plays a key regulatory role in the redox signalling cascade following endogenous ROS generation.
Article
Biochemistry & Molecular Biology
Daniel J. Lagal, Maria J. Lopez-Grueso, Jose R. Pedrajas, Thomas L. Leto, J. Antonio Barcena, Raquel Requejo-Aguilar, C. Alicia Padilla
Summary: PRDX6, a member of the peroxiredoxin family, exhibits peroxidase, phospholipase A2 (PLA2), and lysophosphatidylcholine (LPC) acyltransferase (LPCAT) activities. Knockout of PRDX6 in SNU475 hepatocarcinoma cells leads to lipid peroxidation, mitochondrial dysfunction, metabolic reprogramming, altered cytoskeleton, and down-regulation of growth-related proteins. Activation of upstream regulators and inhibition of migration and invasiveness suggest the involvement of PRDX6 in tumor development and metastasis.
Article
Multidisciplinary Sciences
Alfonso Olaya-Abril, Jesus Hidalgo-Carrillo, Victor M. Luque-Almagro, Carlos Fuentes-Almagro, Francisco J. Urbano, Conrado Moreno-Vivian, David J. Richardson, Maria Dolores Roldan
Summary: The impact of pH on the proteome of the soil denitrifying bacterium Paracoccus denitrificans was studied in this work. Lower pH resulted in increased nitrous oxide emissions and adversely affected the activity of enzymes involved in denitrification and nitrogen assimilation.
SCIENTIFIC REPORTS
(2021)