期刊
ANIMAL GENETICS
卷 48, 期 2, 页码 242-244出版社
WILEY
DOI: 10.1111/age.12515
关键词
genetic engineering; genome editing; Ovis aries; phenotype; beta-carotene oxygenase 2
资金
- Tianyuan Tan Sheep Farm [TYRP201501]
- National Natural Science Foundation of China [31372279, 31171377]
- China Agriculture Research System [CARS-40-13]
The recently emerged CRISPR/Cas9 approach represents an efficient and versatile genome editing tool for producing genetically modified animals. B-carotene oxygenase 2 (BCO2) is a key enzyme in the progress of beta-carotene metabolism and is associated with yellow adipose tissue color in sheep. We have recently demonstrated targeted multiplex mutagenesis in sheep and have generated a group of BCO2-disrupted sheep by zygote injection of the CRISPR/Cas9 components. Here, we show that biallelic modification of BCO2 resulted in yellow fat, compared with the fat color in monoallelic individuals and wild types (snowflower white). We subsequently characterized the effects of gene modifications at genetic levels employing sequencing and Western blotting, highlighting the importance of the BCO2 gene for the determination of fat color in sheep. These results indicate that genetic modification via CRISPR/Cas9 holds great potential for validating gene functions as well as for generating desirable phenotypes for economically important traits in livestock.
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