期刊
MEDICAL SCIENCE MONITOR
卷 25, 期 -, 页码 1671-1678出版社
INT SCIENTIFIC INFORMATION, INC
DOI: 10.12659/MSM.915242
关键词
Diabetic Nephropathies; Inflammation; Medicine, Chinese Traditional
资金
- National Natural Science Foundation of China [81873609, 81600523, 81700617]
Background: Diabetic nephropathy (DN), which is one of the primary causes of end-stage renal disease (ESRD), is increasingly diagnosed in patients due to the continuous increase in the prevalence of diabetic mellitus (DM). Astragali Radix, a traditional Chinese herb, is widely administrated to ameliorate the symptoms of diabetes and diabetic nephropathy, but its mechanism is still not yet fully defined. Calycosin (C16H12O5) is the major active component of Astragali Radix. In this study, we analyzed the role of calycosin in diabetic nephropathy and explored its underlying mechanism. Material/Methods: Cell activation, inflammatory cytokines expression and secretion, and protein levels were analyzed in cultured mouse tubular epithelial cells (mTEC). db/db mice were intraperitoneally injected with 10 mg/(kg.d) calycosin or control saline for 4 weeks, followed by analysis of structure injury, inflammation, and NE-kappa B signaling activity. Results: Our results indicated that TNF-alpha and IL-1 beta were significantly induced by advanced glycation end-products (AGEs), but calycosin remarkably reduced the expression of TNF-alpha and IL-1 beta in the cultured mouse tubular epithelial cells (mTEC). Calycosin effectively alleviated kidney injury in diabetic kidneys of db/db mice during the progression of diabetic renal injury, indicated by the reduction of histological injury and immunohistochemical of inflammatory cytokines. Mechanistically, we identified calycosin inhibited diabetes-induced inflammation in kidneys by suppressing the phosphorylation of IKB alpha and NE-kappa B p65 in vitro and in vivo. Conclusions: Calycosin significantly ameliorated diabetes-induced renal inflammation in diabetic renal injury by inhibition of the NE-kappa B-dependent signaling pathway in vivo and in vitro.
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