Article
Multidisciplinary Sciences
Liam C. Hunt, Bronwen Schadeberg, Jared Stover, Benard Haugen, Vishwajeeth Pagala, Yong-Dong Wang, Jason Puglise, Elisabeth R. Barton, Junmin Peng, Fabio Demontis
Summary: The study found that levels of the ubiquitin ligase UBR4 increase in skeletal muscle with aging, leading to increased proteolytic activity of the proteasome. While muscle-specific loss of UBR4 rescued age-associated myofiber atrophy in mice, it also reduced muscle specific force and accelerated decline in muscle protein quality. This suggests that UBR4 plays a role in regulating myofiber size and muscle protein quality control.
NATURE COMMUNICATIONS
(2021)
Review
Geriatrics & Gerontology
Agnes Martin, Yann S. Gallot, Damien Freyssenet
Summary: Cancer cachexia is a systemic syndrome that leads to skeletal muscle wasting, decreases quality of life and reduces lifespan of cancer patients. This review compares the molecular mechanisms regulating skeletal muscle mass in human cachectic cancer patients and animal models of cancer cachexia. It discusses the regulation of protein turnover, proteolytic capacity, and various regulatory pathways involved in cachexia. The differences in molecular responses between human and animal models are highlighted, and potential therapeutic targets for skeletal muscle wasting in cancer patients are identified.
JOURNAL OF CACHEXIA SARCOPENIA AND MUSCLE
(2023)
Article
Multidisciplinary Sciences
Jiyun Jung, Jangwook Lee, Jeong-Hoon Lim, Yong Chul Kim, Tae Hyun Ban, Woo Yeong Park, Kyeong Min Kim, Kipyo Kim, Sung Woo Lee, Sung Joon Shin, Seung Seok Han, Dong Ki Kim, Yousun Ko, Kyung Won Kim, Hyosang Kim, Jae Yoon Park
Summary: This study investigated the impact of muscle mass on mortality in patients with acute kidney injury requiring continuous renal replacement therapy. It was conducted in eight medical centers between 2006 and 2021, and retrospective data of 2200 patients over 18 years old were collected. The study found that increased skeletal muscle areas/body mass index were associated with decreased mortality risk, and a 26% decreased risk of low attenuation muscle area/body mass index on mortality was identified.
SCIENTIFIC REPORTS
(2023)
Article
Geriatrics & Gerontology
Ruiqin Zhang, Qiang Shen, Yueping Wang, Xue Deng, Jialing Fan, Xiaofan Gu, Meng Fan, Kun Wei, Chun-Ru Cheng, Wei-Dong Zhang, Xiong-wen Zhang, Xuan Liu
Summary: CYA alleviates cancer cachexia muscle atrophy by inhibiting UPS degradation and autophagy. The protective effects of CYA on muscle atrophy may be attributed to its binding with TAOK1 and inhibiting the TAOK1/p38-MAPK/FoxO3 pathway.
JOURNAL OF CACHEXIA SARCOPENIA AND MUSCLE
(2023)
Article
Biochemistry & Molecular Biology
Anjali Bhat, Rafay Abu, Sankarasubramanian Jagadesan, Neetha Nanoth Vellichirammal, Ved Vasishtha Pendyala, Li Yu, Tara L. Rudebush, Chittibabu Guda, Irving H. Zucker, Vikas Kumar, Lie Gao
Summary: Exercise training improves skeletal muscle health through various adaptative pathways. Nrf2, an important antioxidant transcription factor, is crucial for the adaptative responses and beneficial effects of exercise on muscle. The study suggests that Nrf2 plays a critical role in the adaptive effects of skeletal muscle and its response to exercise training.
Article
Biochemistry & Molecular Biology
Javier Botella, Nicholas A. Jamnick, Cesare Granata, Amanda J. Genders, Enrico Perri, Tamim Jabar, Andrew Garnham, Michael Lazarou, David J. Bishop
Summary: Autophagy is an important mechanism for cells to degrade old or dysfunctional proteins and organelles. The effects of exercise on autophagosome content markers differ between rodents and humans. The decrease in LC3B-II protein levels induced by exercise in humans does not reflect a decreased autophagy flux.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Cell Biology
Mattia Scalabrin, Viktor Engman, Amanda Maccannell, Annabel Critchlow, Lee D. Roberts, Nadira Yuldasheva, T. Scott Bowen
Summary: In hind limb ischemia (HLI), early-stage ischemic muscles show overt remodeling with excessive autophagy signaling, which normalizes over time. Early activation of the sestrin-AMPK signaling axis may regulate autophagy to stimulate rapid and overt muscle atrophy in HLI, which is normalized within weeks and accompanied by recovery of muscle mass. A complex interplay between Sestrins likely regulates autophagy signaling during early-to-late muscle remodeling in HLI.
AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY
(2022)
Article
Cell Biology
Yusuke Nishimura, Jitpisute Chunthorng-Orn, Samuel Lord, Ibrahim Musa, Peter Dawson, Lars Holm, Yu-Chiang Lai
Summary: In this study, the researchers showed that Atrogin-1 and MuRF1 protein contents are regulated by different mechanisms, specifically the downstream of Akt, and that Atrogin-1 protein content can be regulated by the rapamycin-sensitive mTOR-S6K1-dependent signaling pathway.
AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Patricia Reboucas, Carine Fillebeen, Amy Botta, Riley Cleverdon, Alexandra P. Steele, Vincent Richard, Rene P. Zahedi, Christoph H. Borchers, Yan Burelle, Thomas J. Hawke, Kostas Pantopoulos, Gary Sweeney
Summary: Using global proteomics, we found significant impairment in mitochondrial oxidative phosphorylation in the skeletal muscle of thalassemia mice, along with a shift in muscle fiber types and increased capillary density. These alterations led to metabolic dysfunction and reduced glucose handling capacity.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Food Science & Technology
Yujie Sun, Qingwei Zheng, Juan Sun, Li Wang, Yan Li
Summary: Autophagy dysfunction is found to be an essential mechanism in the pathogenesis of sarcopenia, and phytonutrients consumption is positively correlated with the maintenance of skeletal muscle mass and strength, involving the regulation of the autophagy pathway. However, the treatment of sarcopenia by targeting autophagy with phytonutrients is sometimes considered controversial due to the opposite effect of autophagy in sarcopenia.
FOOD REVIEWS INTERNATIONAL
(2023)
Article
Cell Biology
Lisa Gambarotto, Samuele Metti, Matteo Corpetti, Martina Baraldo, Patrizia Sabatelli, Silvia Castagnaro, Matilde Cescon, Bert Blaauw, Paolo Bonaldo
Summary: COL6-related myopathies (COL6-RM) are inherited muscle disorders caused by mutations of COL6 genes, characterized by early-onset muscle weakness. Targeting autophagy through dietary spermidine administration has shown beneficial effects in col6a1(-/-) mice and COL6-RM patients. A 100-day-long spermidine regimen rescues muscle strength in col6a1(-/-) mice and improves mitochondria and neuromuscular junction integrity, suggesting its potential application in clinical trials for COL6-RM.
Article
Biochemistry & Molecular Biology
Macsue Jacques, Shanie Landen, Javier Alvarez Romero, Danielle Hiam, Ralf B. Schittenhelm, Iresha Hanchapola, Anup D. Shah, Sarah Voisin, Nir Eynon
Summary: Exercise has significant effects on both the epigenome and proteome in human muscle. A 12-week high-intensity interval training intervention was conducted on 16 healthy moderately trained males, and muscle biopsies were collected at baseline and after 4, 8, and 12 weeks. The study found significant changes in proteins and DNA methylation levels, with some proteins consistently changed across individuals and the discovery of two novel exercise-related proteins. These findings contribute to a better understanding of the impact of exercise on the human body.
Article
Cardiac & Cardiovascular Systems
Shuai Wang, Lin Wang, Shaoke Gu, Yixiao Han, Linfeng Li, Zhuangzhuang Jia, Ning Gao, Yu Liu, Shanshan Lin, Yazhu Hou, Xianliang Wang, Jingyuan Mao
Summary: The study investigated the effect of Chinese medicine, optimized new Shengmai powder, on exercise tolerance in rats with heart failure by regulating the ubiquitin-proteasome signaling pathway. The results showed that the optimized new Shengmai powder could improve cardiac function and exercise tolerance by inhibiting the overactivation of the UPS pathway and exerting anti-apoptotic effects on myocardial and skeletal muscle cells.
FRONTIERS IN CARDIOVASCULAR MEDICINE
(2023)
Article
Biochemistry & Molecular Biology
Manuela Moriggi, Sara Belloli, Pietro Barbacini, Valentina Murtaj, Enrica Torretta, Linda Chaabane, Tamara Canu, Silvia Penati, Maria Luisa Malosio, Antonio Esposito, Cecilia Gelfi, Rosa Maria Moresco, Daniele Capitanio
Summary: The study revealed gender-specific responses to obesity in mice, with males showing various metabolic alterations while females exhibited activation of compensatory mechanisms. Bioinformatics analysis identified upstream molecules regulating pathways differently in males and females.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Food Science & Technology
Wenqiong Chai, Jing Xu, Honglei Qu, Qiugang Ma, Mingxia Zhu, Mengmeng Li, Yandong Zhan, Tianqi Wang, Jingrong Gao, Huanfen Yao, Zeyu Li, Changfa Wang
Summary: This study used quantitative proteomics to investigate differentially abundant proteins in donkey muscles. Several proteins closely related to muscle growth and meat quality were identified, as well as important pathways associated with meat quality.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2022)
Review
Biochemistry & Molecular Biology
Dulce Peris-Moreno, Laura Cussonneau, Lydie Combaret, Cecile Polge, Daniel Taillandier
Summary: Skeletal muscle loss is a harmful side effect of chronic diseases, increasing mortality and morbidity. The ubiquitin proteasome system plays a key role in regulating muscle contraction and metabolism through targeting key proteins for degradation or activity modulation via E3 ubiquitin ligases.
Article
Biochemistry & Molecular Biology
Marine Gueugneau, Cecile Coudy-Gandilhon, Christophe Chambon, Julien Verney, Daniel Taillandier, Lydie Combaret, Cecile Polge, Stephane Walrand, Frederic Roche, Jean-Claude Barthelemy, Leonard Feasson, Daniel Bechet
Summary: The study found that healthy aging in old men is associated with upregulation of apoptosis and immune function, as well as downregulation of glycolysis and protein catabolism, while metabolic syndrome is mainly characterized by upregulation of proteolysis and downregulation of oxidative phosphorylation. This indicates potential muscle biomarkers for healthy aging and metabolic syndrome in old men.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Geriatrics & Gerontology
Giorgio Varesco, Cecile Coudy-Gandilhon, Thomas Lapole, Alice Decourt, Marine Gueugneau, Jean-Claude Barthelemy, Frederic Roche, Daniel Bechet, Leonard Feasson, Vianney Rozand
Summary: The study found that physical activity level was positively associated with the capacity of very old women to perform a fatiguing test, while the physical activity of very old men was not correlated with muscle performance. Increasing steps per day count could provide a sufficient stimulus for adaptations in less active very old women.
JOURNALS OF GERONTOLOGY SERIES A-BIOLOGICAL SCIENCES AND MEDICAL SCIENCES
(2022)
Article
Cell Biology
Laura Cussonneau, Christian Boyer, Charlotte Brun, Christiane Deval, Emmanuelle Loizon, Emmanuelle Meugnier, Elise Gueret, Emeric Dubois, Daniel Taillandier, Cecile Polge, Daniel Bechet, Guillemette Gauquelin-Koch, Alina L. Evans, Jon M. Arnemo, Jon E. Swenson, Stephane Blanc, Chantal Simon, Etienne Lefai, Fabrice Bertile, Lydie Combaret
Summary: Muscle atrophy is a serious condition with detrimental consequences, and there is currently no proven treatment. Research has shown that hibernating brown bears have a natural resistance to muscle atrophy, with an opposite expression pattern of TGF-β/BMP pathways in muscle compared to unloaded mice. Therefore, concurrent activation of the BMP pathway may be a new approach to prevent muscle atrophy.
Article
Cell Biology
Dulce Peris-Moreno, Melodie Malige, Agnes Claustre, Andrea Armani, Cecile Coudy-Gandilhon, Christiane Deval, Daniel Bechet, Pierre Fafournoux, Marco Sandri, Lydie Combaret, Daniel Taillandier, Cecile Polge
Summary: The study identifies that UBE2L3 plays a major role in the degradation of alpha-actin and MHC, as well as in regulating skeletal muscle atrophy. The interactions between MuRF1 and its substrates are found to vary, with different substrates affecting the affinity between MuRF1 and UBE2L3.
Article
Biochemistry & Molecular Biology
Monica Zocchi, Laura Locatelli, Gian Vincenzo Zuccotti, Andre Mazur, Daniel Bechet, Jeanette A. Maier, Sara Castiglioni
Summary: This study found that the concentration of magnesium and its transporters change during myogenic differentiation. MagT1 and SLC41A1 are involved in regulating intracellular magnesium concentration, while TRPM7 is downregulated and undetectable in myotubes. These findings highlight the importance of magnesium in skeletal muscle health.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Immunology
Philippe Nicolas, Jocelyn Ollier, Daiki Mori, Guillaume Voisinne, Javier Celis-Gutierrez, Claude Gregoire, Jeanne Perroteau, Regine Vivien, Mylene Camus, Odile Burlet-Schiltz, Anne Gonzalez de Peredo, Beatrice Clemenceau, Romain Roncagalli, Henri Vie, Bernard Malissen
Summary: Using traceable gene tagging, we investigated the composition and dynamics of TCR-induced signalosomes in human T cells. We found a high degree of conservation in the proximal TCR-signaling network between human CD4(+) and CD8(+) T cells, as well as between human and mouse T cells. Our study suggests that drugs targeting the proximal TCR signaling network should behave similarly when applied to human and mouse T cells. However, differences likely exist in the distal TCR-signaling pathway, and our fast-track AP-MS approach can be favored to determine the mechanism of action of drugs targeting human T cell activation.
JOURNAL OF EXPERIMENTAL MEDICINE
(2022)
Article
Biochemistry & Molecular Biology
Cecile Coudy-Gandilhon, Marine Gueugneau, Christophe Chambon, Daniel Taillandier, Lydie Combaret, Cecile Polge, Guillaume Y. Millet, Leonard Feasson, Daniel Bechet
Summary: Middle-aged and master endurance athletes exhibit similar long-term muscle adaptation to aerobic exercise, but may have distinct short-term plasticity in response to a single bout of prolonged moderate-intensity exercise. This difference may constitute early signs of muscle aging for master athletes.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Multidisciplinary Sciences
Anne Listrat, Kheng Lim Goh, Celine Jousse, Jeremy Tounayre, Huijuan Wang, Kijoon Lee, Daniel Bechet
Summary: This article reports the expression levels of messenger RNA (mRNA) obtained from 18 skeletal muscle samples using Affymetrix Genechips Exon arrays. The dataset includes a large number of mRNA levels and provides insights into the changes in skeletal muscle at different age groups.
Article
Biochemistry & Molecular Biology
Laura Cussonneau, Cecile Coudy-Gandilhon, Christiane Deval, Ghita Chaouki, Mehdi Djelloul-Mazouz, Yoann Delorme, Julien Hermet, Guillemette Gauquelin-Koch, Cecile Polge, Daniel Taillandier, Julien Averous, Alain Bruhat, Celine Jousse, Isabelle Papet, Fabrice Bertile, Etienne Lefai, Pierre Fafournoux, Anne-Catherine Maurin, Lydie Combaret
Summary: Through studies on mice and hibernating brown bears, it is suggested that the induction of muscle atrophy-related genes by activating transcription factor 4 (ATF4) may not be associated with muscle atrophy. In addition, the pharmacological molecule halofuginone can affect the balance of transforming growth factor-β (TGF-β) and bone morphogenetic protein (BMP), thus protecting muscle mass. Therefore, halofuginone may provide a new avenue for combating muscle atrophy.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Nicolas Geraud, Camille Falcou, Julien Parra, Carine Froment, David Rengel, Odile Burlet-Schiltz, Julien Marcoux, Jerome Nigou, Michel Riviere, Emeline Fabre
Summary: Protein-O-mannosyltransferase is crucial for the virulence of Mycobacterium tuberculosis, and inhibitors of this enzyme could potentially improve treatment of mycobacterial diseases. We developed a target-based phenotypic assay and identified compounds that significantly inhibit the enzyme's activity without affecting bacterial growth or mannoprotein secretion.