Article
Genetics & Heredity
Di Liu, Myron F. Goodman, Phuong Pham, Kefei Yu, Chih-Lin Hsieh, Michael R. Lieber
Summary: Activation-induced deaminase (AID) deaminates cytosine within single-stranded DNA. This study found that AID deamination on duplex DNA substrates is reduced when RNase A is added during transcription. The researchers also discovered that AID acts with similar efficiency on RNA-DNA fusion substrates and DNA-only substrates. Based on these findings, they proposed a model in which the mRNA tail plays a critical role in AID loading and collision with DNA strands.
Article
Biochemistry & Molecular Biology
Yanyan Wang, Senxin Zhang, Xinrui Yang, Joyce K. Hwang, Chuanzong Zhan, Chaoyang Lian, Chong Wang, Tuantuan Gui, Binbin Wang, Xia Xie, Pengfei Dai, Lu Zhang, Ying Tian, Huizhi Zhang, Chong Han, Yanni Cai, Qian Hao, Xiaofei Ye, Xiaojing Liu, Jiaquan Liu, Zhiwei Cao, Shaohui Huang, Jie Song, Qiang Pan-Hammarstrom, Yaofeng Zhao, Frederick W. Alt, Xiaoqi Zheng, Lin-Tai Da, Leng-Siew Yeap, Fei-Long Meng
Summary: Complementarity-determining regions (CDRs) in antibody sequences preferentially undergo somatic hypermutation (SHM) due to the flexibility of the surrounding DNA sequence and its interaction with AID protein. This finding has implications for designing animal models for antibody discovery and understanding mutagenesis in lymphoma.
Article
Biotechnology & Applied Microbiology
Tatiana Mitiouchkina, Alexander S. Mishin, Louisa Gonzalez Somermeyer, Nadezhda M. Markina, Tatiana Chepurnyh, Elena B. Guglya, Tatiana A. Karataeva, Kseniia A. Palkina, Ekaterina S. Shakhova, Liliia Fakhranurova, Sofia Chekova, Aleksandra S. Tsarkova, Yaroslav Golubev, Vadim V. Negrebetsky, Sergey A. Dolgushin, Pavel Shalaev, Dmitry Shlykov, Olesya A. Melnik, Victoria O. Shipunova, Sergey M. Deyev, Andrey Bubyrev, Alexander S. Pushin, Vladimir V. Choob, Sergey Dolgov, Fyodor A. Kondrashov, Ilia Yampolsky, Karen S. Sarkisyan
NATURE BIOTECHNOLOGY
(2020)
Article
Plant Sciences
Alexey Pigolev, Dmitry Miroshnichenko, Sergey Dolgov, Tatyana Savchenko
Summary: A well-developed root system is crucial for crop productivity, especially under challenging conditions. Wheat, being a member of Poaceae, has multiple seminal roots whose growth is influenced by environmental factors. Research shows that jasmonates play a role in regulating the development of the sixth seminal root in wheat plants, with effects dependent on hormone concentration and exposure time. The stimulation of sixth seminal root formation peaks at a certain concentration and exposure time, with transgenic lines treated with methyl jasmonate exhibiting significantly higher rates of developed sixth seminal roots compared to non-transgenic plants.
Article
Agriculture, Multidisciplinary
Aleksey Firsov, Lyubov Shaloiko, Oleg Kozlov, Alexander Vainstein, Sergey Dolgov
Summary: This study demonstrates that tomatoes expressing thaumatin II can maintain stability and unique sweetness and taste qualities under processing conditions such as salting and pickling. Salted tomatoes were sweeter than pickled tomatoes, with pickled tomatoes rated as having a better overall taste.
JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
(2021)
Article
Plant Sciences
Tatiana Sidorova, Dmitry Miroshnichenko, Ilya Kirov, Alexander Pushin, Sergey Dolgov
Summary: The study examined the transmission of molecular RNAi silencing signals in transgrafted plum trees and their impact on PPV resistance in genetically modified (GM) and non-transgenic (NT) counterparts. Results showed that while transgenic tissues displayed resistance to PPV, the mobile siRNA signal was not efficiently transferred to the non-transgenic partner, indicating a transitivity failure of the RNAi signal. Further research is needed to understand the complexities of transgrafting in woody fruit trees.
FRONTIERS IN PLANT SCIENCE
(2021)
Article
Biotechnology & Applied Microbiology
Pavel Khvatkov, Alexsey Firsov, Anastasiya Shvedova, Oleg Kozlov, Mariya Chernobrovkina, Alexander Pushin, Lyubov Shaloiko, Sergey Dolgov
Summary: The study successfully transformed 8550 explants with Agrobacterium, resulting in 41 hygromycin-resistant Wolffia lines with a transformation efficiency of 0.48%. 40 of these lines expressed the hirudin-1 gene, with the maximum accumulation of recombinant hirudin being 0.02% of total soluble protein. The results will be used for developing an expression system based on Wolffia plants for producing hirudin and other recombinant pharmaceutical proteins.
Article
Agronomy
Pavel Khvatkov, Vasiliy Taranov, Alexander Pushin, Galina Maletich, Vladislav Fedorov, Inna Chaban, Alexsey Babakov, Sergey Dolgov, Mariya Chernobrovkina
Summary: This study improved the cold and frost resistance of winter rapeseed plants by transferring a new gene Escsdp3 and the traditional cspa gene through genetic engineering. As a result, multiple cold-resistant lines of winter rapeseed were produced, demonstrating enhanced frost resistance in the transgenic plants.
Article
Chemistry, Analytical
Aleksey Firsov, Alexander Pushin, Svetlana Motyleva, Svetlana Pigoleva, Lyubov Shaloiko, Alexander Vainstein, Sergey Dolgov
Summary: Artemisinin-based drugs are the most effective against multidrug-resistant Plasmodium spp., but artemisinin yield depends on agricultural factors and plant genetic variability. Transplanting the artemisinin biosynthetic pathway into chrysanthemums has shown potential for increasing artemisinin content.
Article
Biotechnology & Applied Microbiology
A. P. Firsov, T. Yu. Mitiushkina, A. S. Pushin, A. Tzareva, A. M. Vainstein, S. V. Dolgov
Summary: The study successfully transferred artemisinin biosynthesis genes into transgenic tobacco plants, thereby reconstructing the entire artemisinin biosynthesis pathway in a heterologous host. This will provide important insights for further research on expression systems for the production of artemisinin and other nonprotein substances in heterologous host plants.
APPLIED BIOCHEMISTRY AND MICROBIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Lilia Mourenets, Alexander Pushin, Vadim Timerbaev, Tatyana Khmelnitskaya, Eduard Gribkov, Nikita Andreev, Sergey Dolgov
Summary: Silencing of the eIF(iso)4G and eIF(iso)4E genes through RNA interference can enhance the resistance of sour cherry rootstock 146-2 to sharka disease.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Plant Sciences
Dmitry Miroshnichenko, Vadim Timerbaev, Anna Klementyeva, Alexander Pushin, Tatiana Sidorova, Dmitry Litvinov, Lubov Nazarova, Olga Shulga, Mikhail Divashuk, Gennady Karlov, Elena Salina, Sergey Dolgov
Summary: The VRN-1 gene plays a crucial role in regulating the flowering time of wheat. In this study, the researchers used CRISPR/Cas9 to modify the promoter sequence of the VRN-A1 gene and successfully manipulated the heading time of wheat without affecting its yield characteristics.
FRONTIERS IN PLANT SCIENCE
(2022)