Efficient Targeted Mutagenesis in Apple and First Time Edition of Pear Using the CRISPR-Cas9 System

Optimized paired-sgRNA/Cas9 cloning and expression cassette triggers high-efficiency multiplex genome editing in kiwifruit

(2018) Zupeng Wang et al.   PLANT BIOTECHNOLOGY JOURNAL

Concerns regarding ‘off-target’ activity of genome editing endonucleases

(2018) Ulhas Sopanrao Kadam et al.   PLANT PHYSIOLOGY AND BIOCHEMISTRY

CRISPR/Cas9 editing of carotenoid genes in tomato

(2018) Caterina D’Ambrosio et al.   TRANSGENIC RESEARCH

Transient Expression of CRISPR/Cas9 Machinery Targeting TcNPR3 Enhances Defense Response in Theobroma cacao

(2018) Andrew S. Fister et al.   Frontiers in Plant Science

A method for the production and expedient screening of CRISPR/Cas9-mediated non-transgenic mutant plants

(2018) Longzheng Chen et al.   Horticulture Research

High-quality de novo assembly of the apple genome and methylome dynamics of early fruit development

(2017) Nicolas Daccord et al.   NATURE GENETICS

CRISPR/Cas9-mediated efficient targeted mutagenesis in grape in the first generation

(2017) Xianhang Wang et al.   PLANT BIOTECHNOLOGY JOURNAL

Engineering canker-resistant plants through CRISPR/Cas9-targeted editing of the susceptibility gene CsLOB1 promoter in citrus

(2017) Aihong Peng et al.   PLANT BIOTECHNOLOGY JOURNAL

CRISPR/Cas9-mediated targeted mutagenesis in grape

(2017) Ikuko Nakajima et al.   PLoS One

CRISPR/Cas9 Platforms for Genome Editing in Plants: Developments and Applications

(2016) Xingliang Ma et al.   Molecular Plant

CRISPR/Cas9-mediated efficient targeted mutagenesis in Chardonnay (Vitis vinifera L.)

(2016) Chong Ren et al.   Scientific Reports

Efficient Genome Editing in Apple Using a CRISPR/Cas9 system

(2016) Chikako Nishitani et al.   Scientific Reports

Modification of the PthA4 effector binding elements in Type I CsLOB1 promoter using Cas9/sgRNA to produce transgenic Duncan grapefruit alleviating XccΔpthA4:dCsLOB1.3 infection

(2015) Hongge Jia et al.   PLANT BIOTECHNOLOGY JOURNAL

Heat mediated silencing of MdTFL1 genes in apple (Malus × domestica)

(2015) Kathleen Weigl et al.   PLANT CELL TISSUE AND ORGAN CULTURE

Efficient CRISPR/Cas9-mediated Targeted Mutagenesis in Populus in the First Generation

(2015) Di Fan et al.   Scientific Reports

Both CRISPR/Cas-based nucleases and nickases can be used efficiently for genome engineering inArabidopsis thaliana

(2014) Friedrich Fauser et al.   PLANT JOURNAL

CRISPR/Cas9-mediated targeted mutagenesis in Nicotiana tabacum

(2014) Junping Gao et al.   PLANT MOLECULAR BIOLOGY

Targeted Genome Editing of Sweet Orange Using Cas9/sgRNA

(2014) Hongge Jia et al.   PLoS One

The Draft Genome Sequence of European Pear (Pyrus communis L. ‘Bartlett’)

(2014) David Chagné et al.   PLoS One

Multiplex and homologous recombination–mediated genome editing in Arabidopsis and Nicotiana benthamiana using guide RNA and Cas9

(2013) Jian-Feng Li et al.   NATURE BIOTECHNOLOGY

Plant genome editing made easy: targeted mutagenesis in model and crop plants using the CRISPR/Cas system

(2013) Khaoula Belhaj et al.   Plant Methods

The genome of the pear (Pyrus bretschneideri Rehd.)

(2012) J. Wu et al.   GENOME RESEARCH

Efficient virus-induced gene silencing in apple, pear and Japanese pear using Apple latent spherical virus vectors

(2011) Shintarou Sasaki et al.   Plant Methods

Development of a transgenic early flowering pear (Pyrus communis L.) genotype by RNAi silencing of PcTFL1-1 and PcTFL1-2

(2011) Aviad Freiman et al.   PLANTA

The genome of the domesticated apple (Malus × domestica Borkh.)

(2010) Riccardo Velasco et al.   NATURE GENETICS

Four TFL1/CEN-Like Genes on Distinct Linkage Groups Show Different Expression Patterns to Regulate Vegetative and Reproductive Development in Apple (Malus×domestica Borkh.)

(2009) Naozumi Mimida et al.   PLANT AND CELL PHYSIOLOGY