Article
Plant Sciences
J. E. Holmes, H. Sanghera, Zamir K. Punja
Summary: Crown gall, caused by Agrobacterium tumefaciens, was found for the first time on cannabis plants in licensed production facilities in British Columbia, Canada. The incidence of the disease was extremely low, estimated at 0.01% of total plants.
CANADIAN JOURNAL OF PLANT PATHOLOGY
(2023)
Article
Agriculture, Multidisciplinary
Alexander A. Tkachenko, Maria S. Gancheva, Varvara E. Tvorogova, Lavrenty G. Danilov, Alexander V. Predeus, Irina E. Dodueva, Ludmila L. Lutova
Summary: Using RNA-seq, gene expression changes during early stages of crown gall development in radish were analyzed, revealing different pathways upregulated or downregulated in spontaneous tumorous radish compared to non-tumorous lines.
ANNALS OF APPLIED BIOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Matthias Knoedler, Katharina Frank, Lucy Kerpen, Johannes Felix Buyel
Summary: Antibody-drug conjugates (ADCs) have higher therapeutic indices compared to plain monoclonal antibodies (mAbs), but are more complex and costly to synthesize. Recombinant immunotoxins (RITs) consisting of antibody derivatives, linkers, and proteinaceous toxins can be produced more easily in plants. In this study, effective RITs targeting CD64 for the treatment of myelomonocytic leukemia were produced in Nicotiana benthamiana and N. tabacum plants and tobacco BY-2 cell-based plant cell packs (PCPs). The accumulation of RITs was optimized to a high yield using a design of experiments approach and an optimized affinity chromatography method. One RIT candidate targeting CD64 was characterized for storage stability and cytotoxicity. Bottlenecks in the plant-based expression platform were identified and critical process parameters for plant-made RITs were assessed.
Article
Plant Sciences
Jie Bai, Tuo Yao, Xiaojun Lan, Yanshan Yang, Zhanjun Wang, Xinyou Wang
Summary: The Chinese dwarf cherry crown gall disease was identified in a nursery base in Ningxia Hui Autonomous Region, China, with a pathogen identified as A. tumefaciens/ biovar 1. The pathogenicity assay showed crown gall symptoms on tomato and sunflower stems after inoculation.
JOURNAL OF PLANT PATHOLOGY
(2022)
Article
Agronomy
Rong Xiao, Xiao-Peng Mu, Jian-Cheng Zhang, Shuai Zhang, Chun-Fen Zhang, Shu Deng, Jun-Jie Du, Peng-Fei Wang
Summary: This study aimed to isolate and identify the pathogens causing crown gall disease in Prunus humilis, as well as study their physiological, phylogenetic, opine type, and biological control characteristics. The major pathogenic strains were identified as Rhizobium rhizogenes and Agrobacterium tumefaciens. Some strains possessed genes related to the traditional opines. However, the current biological control strains showed limited efficacy against the pathogenic strains derived from P. humilis.
EUROPEAN JOURNAL OF PLANT PATHOLOGY
(2023)
Article
Agriculture, Multidisciplinary
Serah Lim, Se Chul Chun, Jin-Won Kim
Summary: This study evaluated the resistance of 58 Korean cultivars and six foreign cultivars to crown gall disease. It found that 24 cultivars, including 22 Korean cultivars and 2 foreign cultivars, showed resistance to the disease. The study also discovered a strong correlation between the gall formation rate and the initial gall formation period.
PLANT PATHOLOGY JOURNAL
(2023)
Article
Multidisciplinary Sciences
Haifa Ben Gharsa, Meriam Bouri, Amira Mougou Hamdane, Christina Schuster, Andreas Leclerque, Ali Rhouma
Summary: The study identifies a potential biological agent, B. velezensis MBY2, that can effectively suppress pathogenic Agrobacterium in plants, significantly reducing pathogen populations in maize and tomato plants. Through experiments, the strain demonstrates antagonistic effects in vitro and in vivo, effectively reducing gall development induced by the pathogen.
Article
Plant Sciences
Nan Xu, Qiyuan Yang, Xiaojing Yang, Mingqi Wang, Minliang Guo
Summary: This study utilized a genome-scale metabolic model to investigate the metabolic characteristics of Agrobacterium tumefaciens and found differences in metabolic activity in different ecological niches. By integrating transcriptome data under tumor conditions, significant changes in metabolic pathways were identified.
MOLECULAR PLANT PATHOLOGY
(2021)
Article
Agronomy
Sameh H. Youseif, Fayrouz H. Abd El-Megeed, Abu Zeid A. Abu Zeid, Rehab A. M. Abd-Elrahman, Akram H. Mohamed, Mohamed A. Khalifa, Saleh A. Saleh
Summary: The effectiveness of Rhizobium inoculation significantly improved nodulation, yield, and nitrogen content of faba beans, especially in salt-affected soils.
ARCHIVES OF AGRONOMY AND SOIL SCIENCE
(2021)
Article
Microbiology
Si-Chong Wang, Ai-Ping Chen, Shu-Jen Chou, Chih-Horng Kuo, Erh-Min Lai
Summary: The T6SS is used by proteobacteria to secrete effector proteins and influence bacterial competition or pathogenesis. In this study, the researchers demonstrated that the T6SS affects disease occurrence and the composition of the microbial community in crown galls.
Article
Biology
Akira Kawaguchi, Teruo Sone, Sunao Ochi, Yosuke Matsushita, Yoshiteru Noutoshi, Mizuho Nita
Summary: This study revealed that the population of genetic group A in Hokkaido, Japan, was significantly related to a range of prefectures, indicating that the epidemic of group A strains originated mainly through soil infection. Additionally, group E strains could have been transported by infected nursery stocks as an infection source.
Article
Plant Sciences
Martina Rovere, Chiara Pucciariello, Claude Castella, Antoine Berger, Marco Forgia, Tran A. A. Guyet, Marc Bosseno, Marie Pacoud, Renaud Brouquisse, Pierdomenico Perata, Alexandre Boscari
Summary: This study revealed that MtERF74 and MtERF75 are important for nitrogen-fixing nodules in legumes, as they regulate the expression of hypoxia-responsive genes and maintain nitrogen fixation activity in nodules.
PLANT CELL AND ENVIRONMENT
(2023)
Article
Agriculture, Multidisciplinary
I. Adem Bozkurt, Soner Soylu, Merve Kara, Senem Filiz Doksoz, Bulent Altan, Hakan Carpar
Summary: The occurrence of Agrobacterium tumefaciens on citrus trees in Turkey was reported for the first time.
JOURNAL OF PLANT DISEASES AND PROTECTION
(2023)
Article
Agriculture, Multidisciplinary
Ting Ting Chen, Fei Ran, Jin Qiao Shi, Fei Xu Mo, Xian Hui Yin, Zhi Bo Zhao, Zhu Zhu Zhang, Wen Zhi Li, Li Nan He, You Hua Long
Summary: In this study, a strain of Bacillus velezensis, designated as JK-F2, was isolated and identified as an antagonistic bacterium against Agrobacterium Fabacearum. The strain exhibited good biocontrol effects both in vitro and in pot tests, significantly reducing the incidence and growth rate of crown gall in kiwifruit plants.
JOURNAL OF PLANT DISEASES AND PROTECTION
(2022)
Article
Plant Sciences
Pao-Yuan Hsiao, Cyong-Yu Zeng, Ming-Che Shih
Summary: The study found that three BdERFVIIs genes are involved in a feedback regulatory loop to mediate downstream responses in the submergence stress response. The same regulatory loop was also found in rice, involving the orthologue of BdERF961. These findings offer new perspectives for the development of submergence-tolerant crops.
Review
Plant Sciences
Francesco Licausi, Beatrice Giuntoli
Summary: Synthetic biology has great potential in the study of plant hypoxia physiology, allowing for the inference of plant oxygen sensing mechanisms and the development of genetic tools for regulating plant responses and metabolism in low oxygen environments.
Review
Plant Sciences
Daan A. Weits, Joost T. van Dongen, Francesco Licausi
Summary: Studies have shown that oxygen plays a crucial role in plant development, with chronic hypoxia being distinct from stress-associated acute hypoxia. The Cys/Arg branch of the N-degron pathway plays a key role in integrating oxygen levels and influencing plant growth and development.
Article
Plant Sciences
Maria Cristina Valeri, Giacomo Novi, Daan A. Weits, Anna Mensuali, Pierdomenico Perata, Elena Loreti
Summary: Infection by Botrytis cinerea induces local hypoxia in leaves, leading to stabilization of ERF-VII proteins. This hypoxic environment may impact the stability of other N-degron-regulated proteins and the metabolism of elicitors.
Editorial Material
Plant Sciences
Francesco Licausi, Beatrice Giuntoli, Pierdomenico Perata
TRENDS IN PLANT SCIENCE
(2020)
Article
Plant Sciences
Khac Nhu Nghi, Andrea Tagliani, Lorenzo Mariotti, Daan A. Weits, Pierdomenico Perata, Chiara Pucciariello
Summary: The availability of auxin in rice coleoptile under submergence correlates with its final length, with the auxin influx carrier AUX1 playing a key role in influencing this trait. The coleoptile tip is involved in determining the final length of rice varieties with long coleoptiles. Experimental results suggest that auxin biosynthesis and transport are underlying factors in the differential elongation between short and long coleoptile-harbouring varieties of japonica rice.
Article
Plant Sciences
Liem T. Bui, Vinay Shukla, Federico M. Giorgi, Alice Trivellini, Pierdomenico Perata, Francesco Licausi, Beatrice Giuntoli
Article
Multidisciplinary Sciences
Mark D. White, Laura Dalle Carbonare, Mikel Lavilla Puerta, Sergio Lacopino, Martin Edwards, Kate Dunne, Elisabete Pires, Colin Levy, Michael A. McDonough, Francesco Licausi, Emily Flashman
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2020)
Review
Multidisciplinary Sciences
Emma U. Hammarlund, Emily Flashman, Sofie Mohlin, Francesco Licausi
Article
Chemistry, Analytical
Gabriele Panicucci, Sergio Iacopino, Elisa De Meo, Pierdomenico Perata, Daan A. Weits
Article
Plant Sciences
Luca Piccinini, Sergio Iacopino, Stefano Cazzaniga, Matteo Ballottari, Beatrice Giuntoli, Francesco Licausi
Summary: Inspired by the light-driven conformational transitions of orange carotenoid proteins, researchers developed a molecular device that can switch its state in response to blue-green light. This study provides a new approach for applying light-responsive systems in plants and successfully controlled transcriptional responses in chloroplasts using a photoswitch.
Article
Plant Sciences
Daan A. Weits, Lina Zhou, Beatrice Giuntoli, Laura Dalle Carbonare, Sergio Iacopino, Luca Piccinini, Lara Lombardi, Vinay Shukla, Liem T. Bui, Giacomo Novi, Joost T. van Dongen, Francesco Licausi
Summary: N-terminal cysteine oxidases (NCOs) oxidise the N-terminal cysteine of specific proteins, initiating proteolytic N-degron pathway. Both types of PCOs regulate the anaerobic response in angiosperms.
PLANT CELL AND ENVIRONMENT
(2023)
Article
Soil Science
Kim Thu Nguyen, Kilian E. C. Smith, Richard Ottermanns, Christiaan Wijntjes, Joost T. van Dongen, Andreas Schaeffer
Summary: The use of pesticides in agriculture is common to prevent crop infestation and increase productivity. However, the combination of different protection products can affect the degradation and metabolism of chemicals in soil, posing challenges for risk assessment on human and environmental exposure.
Editorial Material
Biochemistry & Molecular Biology
Daai Zhang, Francesco Licausi
Article
Multidisciplinary Sciences
Chrysoula K. Pantazopoulou, Sara Buti, Chi Tam Nguyen, Lisa Oskam, Daan A. Weits, Edward E. Farmer, Kaisa Kajala, Ronald Pierik
Summary: Plants detect their neighbors through touch, which triggers a calcium response and drives leaf movement. This touch-induced hyponasty mechanism is adaptive and involves an increase in cytosolic calcium concentration in leaf tip trichomes.
NATURE COMMUNICATIONS
(2023)